Team:Cornell/Notebook/Salicylate reporter

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June

Week

June 29th, Friday

  • Began preparation for Vent PCR(DPW) at 10:56 AM
    • Dylan's magic triple anneal method (55/60/63).
  • Thermal cycler started at 11:00 am.
  • Gel purified PCR product.
  • Miniprepped (STC) overnight cultures of PL0014-PL0020
    • Using C1015 rotor, 6666 x g, the Corning culture tubes only fit in the centrifuge with the lids off
  • Made LB, 3x 60 mL in 100 mL bottles (SS)
  • Made LB Agar, 4 x 250 mL LB Agar in 500 mL bottles (CS)
  • Autoclaved LB, LB Agar, and milliQ (ss)