Team:Cornell/Notebook/Salicylate reporter

From 2012.igem.org

(Difference between revisions)
(June 29th, Friday)
(June 29th, Friday)
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** Dylan's magic triple anneal method (55/60/63)
** Dylan's magic triple anneal method (55/60/63)
* Gel purified PCR product.
* Gel purified PCR product.
-
* [[Miniprep]]ped overnight cultures of PL0014-PL0020 (STC)
+
* [[Miniprep]]ped overnight cultures of PL14-PL20 (STC)
** Using C1015 rotor, 6666 x g, the Corning culture tubes only fit in the centrifuge with the lids off
** Using C1015 rotor, 6666 x g, the Corning culture tubes only fit in the centrifuge with the lids off
* Made [[LB]], 3x 60 mL in 100 mL bottles (SS)
* Made [[LB]], 3x 60 mL in 100 mL bottles (SS)
* Made [[LB|LB Agar]], 4 x 250 mL LB Agar in 500 mL bottles (CS)
* Made [[LB|LB Agar]], 4 x 250 mL LB Agar in 500 mL bottles (CS)
* [[Autoclave]]d LB, LB Agar, and milliQ (SS)
* [[Autoclave]]d LB, LB Agar, and milliQ (SS)
-
* [[Making LB Plates]] with Kan (cmr)
+
* Made [[LB]] plates with Kan (CMR)

Revision as of 22:02, 29 June 2012

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June

Week

June 29th, Friday

  • Vent PCR at 11:00 (DPW)
    • Dylan's magic triple anneal method (55/60/63)
  • Gel purified PCR product.
  • Miniprepped overnight cultures of PL14-PL20 (STC)
    • Using C1015 rotor, 6666 x g, the Corning culture tubes only fit in the centrifuge with the lids off
  • Made LB, 3x 60 mL in 100 mL bottles (SS)
  • Made LB Agar, 4 x 250 mL LB Agar in 500 mL bottles (CS)
  • Autoclaved LB, LB Agar, and milliQ (SS)
  • Made LB plates with Kan (CMR)