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Team:Cornell/Notebook/Nah operon/June24
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====June 27th, Wednesday==== | ====June 27th, Wednesday==== | ||
[[Image:2012_627_RafDylanGelMaking.jpg|thumb|right|Rafael and Dylan making an agarose gel.]] | [[Image:2012_627_RafDylanGelMaking.jpg|thumb|right|Rafael and Dylan making an agarose gel.]] | ||
| - | *Ran digest of gibson-assembled nah operon with [[gel electrophoresis]] | + | *Ran digest of gibson-assembled nah operon with [[Team:Cornell/Notebook/Gel_electrophoresis|gel electrophoresis]] |
====June 28th, Thursday==== | ====June 28th, Thursday==== | ||
Revision as of 21:39, 3 July 2012
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The purpose of this subproject is to make the nah operon biobrick compatible for the use of future iGEM teams. The nah operon codes for a pathway that converts naphthalene into salicylate. In our system, this will allow naphthalene to be detected by the salicylate reporter. There are three standard cut sites internal to the operon which we are removing - PstI, and two NotI sites. This biobrick compatible piece could be useful to future teams in projects that involve bioremediation or detection of naphthalene. Back to nah operon week view
June 24th-30th
June 27th, Wednesday
- Ran digest of gibson-assembled nah operon with gel electrophoresis
====June 28th, Thursday====
