Team:Cornell

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== <center>'''Our wiki is currently under construction - come back soon for more project details!'''</center>==
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<a href="https://2012.igem.org/Team:Cornell/project"><img src="https://static.igem.org/mediawiki/2012/1/13/Cornell_Dan_SQ.jpg"></a>
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<a href="https://2012.igem.org/Team:Cornell/project"><h3 style="margin:0px auto 0px;">Project</h3></a>
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Discover our novel approach to environmental biosensing, where synthetic biology intersects with microbial electrochemical systems.
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<a href="https://2012.igem.org/Team:Cornell/project" class="small button radius">More Info</a>
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<a href="https://2012.igem.org/Team:Cornell/notebook"><img src="https://static.igem.org/mediawiki/2012/9/9f/Cornell_notebook_square.jpg"></a>
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See how our wetlab and drylab subteams operated on a day-to-day basis in order to accomplish our goals of constructing a field-deployable biosensor with direct electric output.
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Canadian oil sands are a vast oil reserve that, given rising prices of petroleum, are an attractive alternative to traditional sources of crude oil. However, there are numerous public health and environmental concerns regarding the oil sands extraction process. One environmental concern is the contamination of Canadian watersheds by seepage from tailings ponds. To better monitor this issue, we will engineer a novel biosensing platform with the electroactive bacterial species Shewanella oneidensis MR-1, which has the unique capability to directly transfer electrons to solid-state electrodes. We plan to exploit this feature by genetically manipulating S. oneidensis MR-1 to upregulate its metal- reduction capacity in the presence of analyte to generate direct current output in a whole-cell biosensor. Our goal is to develop a fully autonomous electrochemical biosensor that complements the current oil sands monitoring system by providing real-time data over extended periods of time. Furthermore, our device will circumvent the costs and complications of producing and maintaining photodiode circuits used for data acquisition in bioluminescent reporter systems by instead producing a direct electrical output. While our platform is adaptable to sensing a wide range of analytes, we will initially focus on arsenic-containing compounds and naphthalene, a polycyclic aromatic hydrocarbon (PAH) – known contaminants of oil sands tailings ponds. We believe that our biosensor will be a valuable tool for remote, continuous, and long-term monitoring of pollutants in rivers and key waterways.
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<a href="https://2012.igem.org/Team:Cornell/notebook" class="small button radius corner">More Info</a>
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<a href="https://2012.igem.org/Team:Cornell/team"><img src="https://static.igem.org/mediawiki/2012/5/50/Team_square.jpg"></a>
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Meet our dedicated team of undergraduates who conceived of, planned, and carried out all the biological, mechanical and electrical engineering to complete our device.
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In order to build and test this device, we plan to:  
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: Synthesize novel reporter strains for the production of electrical output in response to arsenic and the PAH naphthalene.  
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: Characterize electrical output of reporter strains in response to the pollutant of interest.  
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: Optimize this response for relevant concentrations of pollutant in water samples.  
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: Construct a functional prototype for an affordable, field deployable device.
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<a href="https://2012.igem.org/Team:Cornell/team" class="small button radius corner">More Info</a>
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<a href="https://2012.igem.org/Team:Cornell/outreach"><img src="https://static.igem.org/mediawiki/igem.org/3/3b/Swati_square.jpg"></a>
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Examine our efforts to reach out to our local community &#8212; involving presentations to other scientists, discussions with fellow students and interaction with educators and young children.
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<a href="https://2012.igem.org/Team:Cornell/outreach" class="small button radius corner">More Info</a>
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Latest revision as of 23:51, 15 November 2012

Project
Discover our novel approach to environmental biosensing, where synthetic biology intersects with microbial electrochemical systems.
More Info

Notebook
See how our wetlab and drylab subteams operated on a day-to-day basis in order to accomplish our goals of constructing a field-deployable biosensor with direct electric output.
More Info

Team
Meet our dedicated team of undergraduates who conceived of, planned, and carried out all the biological, mechanical and electrical engineering to complete our device.
More Info

Outreach
Examine our efforts to reach out to our local community — involving presentations to other scientists, discussions with fellow students and interaction with educators and young children.
More Info

Retrieved from "http://2012.igem.org/Team:Cornell"