Team:Columbia-Cooper-NYC/trial

From 2012.igem.org

(Difference between revisions)
Line 12: Line 12:
<DIV STYLE="position:absolute; top:410px; left:155px; width:840px; height:25px";>
<DIV STYLE="position:absolute; top:410px; left:155px; width:840px; height:25px";>
-
<FONT SIZE="+1" COLOR="000000">* Met with Jason, who showed us how to make A. ferrooxidans liquid media, prepared Copper 1 and 2  
+
<FONT SIZE="+1" line-height="12px" COLOR="000000">* Met with Jason, who showed us how to make A. ferrooxidans liquid media, prepared Copper 1 and 2 samples (with and without bacteria) and placed on shaker. Will be periodically checked until June 25 twice a day for activity.</FONT></DIV>
-
 
+
-
samples (with and without bacteria) and placed on shaker. Will be periodically checked until June 25 twice a day for activity.</FONT></DIV>
+

Revision as of 00:43, 13 August 2012

Notebook Background.jpg

Copper Experiments 1
Friday, June 22nd
Outline of activity today:
* Met with Jason, who showed us how to make A. ferrooxidans liquid media, prepared Copper 1 and 2 samples (with and without bacteria) and placed on shaker. Will be periodically checked until June 25 twice a day for activity.
* Showed us recipe for solid media, and made enough for two samples, 20 mL (one control with no bacteria). Copper samples were submerged in agarose (see Steven’s pictures). Also learned protocols listed below. Starting Sample masses: {| class="wikitable" style="margin: 1em auto 1em auto;" |+ '''Copper 1: (liquid media only)''' ! scope="col" | Sample ! scope="col" | Mass (g) |- | 1 || 0.0092 |- | 2 || 0.0107. |} {| class="wikitable" style="margin: 1em auto 1em auto;" |+ '''Copper 2: (liquid media only)''' ! scope="col" | Sample ! scope="col" | Mass (g) |- | 1 || 0.1318 |- | 2 || 0.1286. |} {| class="wikitable" style="margin: 1em auto 1em auto;" |+
'''Copper 2: (solid media)'''
! scope="col" | Sample ! scope="col" | Mass (g) |- | 1 || 0.1272 |- | 2 || 0.1282. |} Liquid media and flasks were placed in shaker at 11:35 am, 225 rpm, 30C. Solid media and petri dishes were placed in incubator at 12:42 pm, 30C.