Team:Columbia-Cooper-NYC/Ligation
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(Created page with "= Ligation Protocol for 3A Assembly Method = * The following protocol is provided by bioworks: # Add 11µl of denionized water to 200µl PCR tube # Add 2µl of each of the digest...")
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(Created page with "= Ligation Protocol for 3A Assembly Method = * The following protocol is provided by bioworks: # Add 11µl of denionized water to 200µl PCR tube # Add 2µl of each of the digest...")
Newer edit →
Revision as of 02:17, 3 October 2012
Ligation Protocol for 3A Assembly Method
- The following protocol is provided by bioworks:
- Add 11µl of denionized water to 200µl PCR tube
- Add 2µl of each of the digests to the tube
- Add 2µl of 10X T4 DNA Ligase Reaction Buffer to tube
- Add 1µl of the T4 DNA Ligase to the tube
- Mix solution well
- Incubate the reaction mix at room temperature for 10 minutes
- Incubate the reaction mix at 80C for 20 minutes to inactivate enzymes
- Store ligation mix at -20C