Team:Carnegie Mellon/Notebook/4 June 2012
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* Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively. | * Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively. | ||
- | + | * Harvested DH5-alpha cells from stock culture and made cells competent. | |
- | + | * Followed transformation protocol from Dr. Doonan’s lab (see Protocols) | |
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Latest revision as of 21:01, 26 June 2012
June 04, 2012:
- Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively.
- Harvested DH5-alpha cells from stock culture and made cells competent.
- Followed transformation protocol from Dr. Doonan’s lab (see Protocols)