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Team:Carnegie Mellon/Notebook/4 June 2012
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* Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively. | * Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively. | ||
| - | + | ** Harvested DH5-alpha cells from stock culture and made cells competent. | |
| - | + | ||
| - | + | ||
*** Followed transformation protocol from Dr. Doonan’s lab (see Protocols) | *** Followed transformation protocol from Dr. Doonan’s lab (see Protocols) | ||
Revision as of 20:59, 26 June 2012
6/04
- Transformation of Charlie and dNP138 plasmid, obtained from MBIC doctoral students, Bradley and Kalin respectively.
- Harvested DH5-alpha cells from stock culture and made cells competent.
- Followed transformation protocol from Dr. Doonan’s lab (see Protocols)
- Harvested DH5-alpha cells from stock culture and made cells competent.
