Team:Carnegie Mellon/Modelling


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Project Description

The model is composed in Matlab. It incorporates file input/output to retrieve measurement data to approximate two important characteristics of gene expression, PoPS and translational efficiency. The inputs to the model are time dependent and dye-dependent fluorescence measurements of mRNA and protein. Four time dependent measurements are needed: mRNA fluorescence during synthesis, mRNA fluorescence during degradation (no mRNA production), protein fluorescence during synthesis, and protein fluorescence during degradation (no protein production). Other constants are approximated as needed.

The output values, PoPS and translational efficiency, will help characterize promoters that will be used in the experiments. These parameters are notoriously difficult to measure consistently in vivo. In addition to the two main output values, the model also calculates other characteristics of the cell, such as degradation constants for mRNA and protein, and transcriptional efficiency.