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Team:Carnegie Mellon/Bio-Submitted
From 2012.igem.org
Submitted Parts
BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA
Mutant II: CAATACGACTCACTATAGGGCAATTGTGAGCGCTCACAATTGCAA
Mutant III: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA
Mutant IV: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA
Hypothesis:
Expected promoter strength of different mutants:Mutant I: <100%
Mutant II: >>100%
Mutant III: ~100%
Mutant IV: ~50%
Expected LacI leaky expression of different mutants:
Mutant I: More than average
Mutant II: Below average
Mutant III: Average
Mutant IV: Average
