Team:Carnegie Mellon/Bio-Submitted

From 2012.igem.org

(Difference between revisions)
Line 117: Line 117:
BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA <br>
BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA <br>
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA <br>
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA <br>
-
Mutant II: CAATACGACTCACTATAGGGCAATTGTGAGCGCTCACAATTGCAA <br>
+
Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA <br>
-
Mutant III: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA <br>
+
Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA <br>
-
Mutant IV: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA <br>
+
</p><p class="promoter">
</p><p class="promoter">
<h3 class="pre-experiment">Hypothesis:</h3>
<h3 class="pre-experiment">Hypothesis:</h3>
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Expected promoter strength of different mutants:<br>
Expected promoter strength of different mutants:<br>
Mutant I: <100% <br>
Mutant I: <100% <br>
-
Mutant II: >>100% <br>
+
Mutant II: ~100% <br>
-
Mutant III: ~100% <br>
+
Mutant III: ~50%<br>
-
Mutant IV: ~50%<br>
+
</p><p class="leaky">
</p><p class="leaky">
Expected LacI leaky expression of different mutants: <br>
Expected LacI leaky expression of different mutants: <br>
Mutant I: More than average <br>
Mutant I: More than average <br>
-
Mutant II: Below average <br>
+
Mutant II: Average <br>
Mutant III: Average <br>
Mutant III: Average <br>
-
Mutant IV: Average <br>
 
</p>
</p>
</html>
</html>
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Revision as of 21:56, 19 September 2012

Image:CMU_image6.jpeg




Submitted Parts

BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA
Mutant II: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA
Mutant III: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA

Hypothesis:

Expected promoter strength of different mutants:
Mutant I: <100%
Mutant II: ~100%
Mutant III: ~50%

Expected LacI leaky expression of different mutants:
Mutant I: More than average
Mutant II: Average
Mutant III: Average

Image:TartanFooter.jpeg

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