Team:Carnegie Mellon

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Carnegie Mellon iGEM 2012


Welcome to Carnegie Mellon University 2012 iGEM Team Wiki!

Image:Cmu2.jpeg

 

Contents

Introduction: Motivation


Abstract/Introduction

Motivation question

Humanistic implications go here

Primary Objective: A Useful BioBrick for Synthetic Biologists

Fluorescence Mircroscopy
Fluorescence Microscopy

We believe the development of this unprecedented BioBrick will help synthetic biologists in a variety of applications, for a variety of purposes such as the following:

  1. Quantifying translational efficiency in vivo
  2. Troubleshooting in expression strains
  3. mRNA and protein localization
  4. in vivo transcription rate analysis
  5. Determining promoter strength in vivo
  6. in vivo mRNA and protein half-lives
  7. Introducing a protein reporter that has virtually no maturation rate and is limited only by the very quick absorption rate of the fluorogen into the cell
  8. Introducing a functioning mRNA reporter
  9. Providing a method to better characterize current and future BioBricks

Our proposed BioBrick is novel, and potentially very useful in practice.

Secondary Objective: Humanistic Practice

FAQ/Terminology in engineering Escherichia coli to monitor these variables via fluorescence. Find out more about Carnegie Mellon: (CMU Home Page).

Further Considerations

In the pursuit of our project, as well as the biological aspects, we:

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