Team:Carnegie Mellon

From 2012.igem.org

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<a name="Primary_Objective:_A_Useful_BioBrick_for_Synthetic_Biologists"></a><h2> <span class="mw-headline"> Primary Objective: A Useful BioBrick for Synthetic Biologists </span></h2>
<a name="Primary_Objective:_A_Useful_BioBrick_for_Synthetic_Biologists"></a><h2> <span class="mw-headline"> Primary Objective: A Useful BioBrick for Synthetic Biologists </span></h2>
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<div class="thumb tright"><div class="thumbinner" style="width:152px;"><a href="/Image:Carnegie_Mellon-MicroMaize.jpg" class="image" title="A false-colour SEM of MicroMaize cells."><img alt="A false-colour SEM of MicroMaize cells." src="/wiki/images/thumb/0/08/Carnegie_Mellon-MicroMaize.jpg/150px-Carnegie_Mellon-MicroMaize.jpg" width="150" height="301" border="0" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/Image:Carnegie_Mellon-MicroMaize.jpg" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>A false-colour SEM of MicroMaize cells.</div></div></div>
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<div class="thumb tright"><div class="thumbinner" style="width:152px;"><a href="/Image:Carnegie_Mellon-MicroMaize.jpg" class="image" title="Fluorescence Microscopy"><img alt="Fluorescence Mircroscopy" src="/wiki/images/thumb/0/08/Carnegie_Mellon-MicroMaize.jpg/150px-Carnegie_Mellon-MicroMaize.jpg" width="150" height="301" border="0" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/Image:Carnegie_Mellon-MicroMaize.jpg" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fluorescence Microscopy</div></div></div>
<p>We believe the development of this unprecedented BioBrick will help synthetic biologists in a variety of applications,  <b>for a variety of purposes </b> such as the following:
<p>We believe the development of this unprecedented BioBrick will help synthetic biologists in a variety of applications,  <b>for a variety of purposes </b> such as the following:
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Revision as of 11:46, 11 June 2012

Team:Carnegie_Mellon - 20012.igem.org

Team:Carnegie_Mellon

From 2012.igem.org


Contents

Introduction: Motivation

  • We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure translational efficiency, and transcriptional strength.
  • We believe that we can use Spinach as a biosensor to reflect these metrics in vivo, rather than in vitro, which has previously proven to be very costly and impractical.
  • We will characterize the relationship between genetic expression of Spinach (upstream), translational efficiency, and transcriptional strength.


Abstract/Introduction

Motivation question

Humanistic implications go here

Primary Objective: A Useful BioBrick for Synthetic Biologists

Fluorescence Mircroscopy
Fluorescence Microscopy

We believe the development of this unprecedented BioBrick will help synthetic biologists in a variety of applications, for a variety of purposes such as the following:

  1. example application
  2. example application
  3. example application

Our proposed BioBrick is novel, and potentially very useful in practice.

Secondary Objective: Humanistic Practice

FAQ/Terminology in engineering Escherichia coli to monitor these variables via fluorescence. Find out more about Carnegie Mellon: (CMU Home Page).

Further Considerations

In the pursuit of our project, as well as the biological aspects, we: