Team:Carnegie Mellon

From 2012.igem.org

(Difference between revisions)
Line 464: Line 464:
<p>In the pursuit of our project, as well as the biological aspects, we:
<p>In the pursuit of our project, as well as the biological aspects, we:
</p>
</p>
-
<ul><li> considered aspects of <a href="https://2012.igem.org/Team:Carnegie_Mellon/Modelling" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Modelling" rel="nofollow">scale-up</a>, including the <a href="https://2012.igem.org/Team:Carnegie_Mellon" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon" rel="nofollow">ethical, legal and social implications</a> of our BioBrick,
+
<ul><li> Considered aspects of <a href="https://2012.igem.org/Team:Carnegie_Mellon/Modelling" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Modelling" rel="nofollow">scale-up</a>, including the <a href="https://2012.igem.org/Team:Carnegie_Mellon" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon" rel="nofollow">ethical, legal and social implications</a> of our BioBrick,
</li><li> Programmed <a href="https://2012.igem.org/Team:Carnegie_Mellon/Software" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Software" rel="nofollow">a new piece of software</a> for modeling our BioBrick to students,
</li><li> Programmed <a href="https://2012.igem.org/Team:Carnegie_Mellon/Software" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Software" rel="nofollow">a new piece of software</a> for modeling our BioBrick to students,
</li><li> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Protocols" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Protocols" rel="nofollow">Developed and tested techniques for measuring translational efficiency and transcriptional strength,
</li><li> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Protocols" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Protocols" rel="nofollow">Developed and tested techniques for measuring translational efficiency and transcriptional strength,

Revision as of 11:42, 11 June 2012

Team:Carnegie_Mellon - 20012.igem.org

Team:Carnegie_Mellon

From 2012.igem.org


Contents

Introduction: Motivation

  • We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure translational efficiency, and transcriptional strength.
  • We believe that we can use Spinach as a biosensor to reflect these metrics in vivo, rather than in vitro, which has previously proven to be very costly and impractical.
  • We will characterize the relationship between genetic expression of Spinach (upstream), translational efficiency, and transcriptional strength.


Abstract/Introduction

Motivation question

Humanistic implications go here

Primary Objective: A Useful BioBrick for Synthetic Biologists

A false-colour SEM of MicroMaize cells.
A false-colour SEM of MicroMaize cells.

We believe the development of this unprecedented BioBrick will help synthetic biologists in a variety of applications, for a variety of purposes such as the following:

  1. example application
  2. example application
  3. example application

Our proposed BioBrick is novel, and potentially very useful in practice.

Secondary Objective: Humanistic Practice

FAQ/Terminology in engineering Escherichia coli to monitor these variables via fluorescence. Find out more about Carnegie Mellon: (CMU Home Page).

Further Considerations

In the pursuit of our project, as well as the biological aspects, we: