Team:Cambridge/Week 3/Lab book

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Diary Lab book


Contents

Monday

Making bacillus competent


Bacillus salts *10 made up:

  • (NH4)2SO4: 20g
  • KH2PO4 (anhydrous): 60g
  • Anhydrous K2HPO4 189.7g (N.B. Different from the one below!)
  • KH2PO4 60g
  • Tri-sodium citrate 10g
  • MgSO4•7H2O 2g
  • Added distilled water to make 1000mL
  • Autoclaved

Medium A base *10 made up:

  • Yeast extract 10g
  • Casamino acids 2g
  • Added distilled water to make 900mL
  • Autoclaved

bacillus strain 168 streaked out and grown on plate overnight.

Tuesday

Making bacillus competent


Wednesday

Making bacillus competent


Thursday

Test transformation of frozen bacillus stocks


Friday

Transformation of bacillus and e.coli with lux genes from 2010


Testing of the spectral properties of filters