Team:Cambridge/Protocols/biobrick protocols
From 2012.igem.org
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#Pierce foil of required well and pipette 10µL dH<sub>2</sub>O (distilled water). | #Pierce foil of required well and pipette 10µL dH<sub>2</sub>O (distilled water). | ||
#Pipette up and down several times to fully resuspend the DNA sample. Sit for 10 minutes. | #Pipette up and down several times to fully resuspend the DNA sample. Sit for 10 minutes. | ||
- | #The BioBrick is now ready for [[Team:Cambridge/Protocols/TransformationofE.coli| transformation]] | + | #The BioBrick is now ready for [[Team:Cambridge/Protocols/TransformationofE.coli#Practice| transformation]] |
==Linearised Plasmid Backbones== | ==Linearised Plasmid Backbones== |
Revision as of 20:57, 23 August 2012
BioBricks
Distribution Kit page A link to the Registry of Standard Biological Parts
Resuspension of BioBricks from the distribution
see Using the Spring 2012 DNA Distribution for a more detailed protocol!
- Locate your BioBrick of interest in the Distribution: Spring 2012 Distribution, DNA Part Repositories Make sure the plate is properly orientated! The two notched corners of the plate are oriented at the BOTTOM of the plate.
- Pierce foil of required well and pipette 10µL dH2O (distilled water).
- Pipette up and down several times to fully resuspend the DNA sample. Sit for 10 minutes.
- The BioBrick is now ready for transformation
Linearised Plasmid Backbones