Team:Cambridge/Protocols/biobrick protocols
From 2012.igem.org
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#The BioBrick is now ready for [[Team:Cambridge/Protocols/TransformationofE.coli#Practice| transformation]] | #The BioBrick is now ready for [[Team:Cambridge/Protocols/TransformationofE.coli#Practice| transformation]] | ||
- | == | + | ==Making Biobricks== |
[http://partsregistry.org/Help:Protocols/Linearized_Plasmid_Backbones Linearised Plasmid Backbone] | [http://partsregistry.org/Help:Protocols/Linearized_Plasmid_Backbones Linearised Plasmid Backbone] | ||
+ | [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication Biobrick assembly] | ||
<center>'''[[Team:Cambridge/Protocols|Back to Protocols]]'''</center> | <center>'''[[Team:Cambridge/Protocols|Back to Protocols]]'''</center> | ||
{{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}} | {{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}} |
Revision as of 13:15, 14 September 2012
BioBricks
Distribution Kit page A link to the Registry of Standard Biological Parts
Resuspension of BioBricks from the distribution
see Using the Spring 2012 DNA Distribution for a more detailed protocol!
- Locate your BioBrick of interest in the Distribution: Spring 2012 Distribution, DNA Part Repositories Make sure the plate is properly orientated! The two notched corners of the plate are oriented at the BOTTOM of the plate.
- Pierce foil of required well and pipette 10µL dH2O (distilled water).
- Pipette up and down several times to fully resuspend the DNA sample. Sit for 10 minutes.
- The BioBrick is now ready for transformation
Making Biobricks
Linearised Plasmid Backbone Biobrick assembly