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Team:Cambridge/Protocols/CCMB80 - Revision history
2024-03-29T00:34:55Z
Revision history for this page on the wiki
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http://2012.igem.org/wiki/index.php?title=Team:Cambridge/Protocols/CCMB80&diff=292884&oldid=prev
Emmyft: /* CCMB80 buffer Recipe */
2012-10-27T00:11:28Z
<p><span class="autocomment">CCMB80 buffer Recipe</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* sterile filter and store at 4&deg;C</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* sterile filter and store at 4&deg;C</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* slight dark precipitate appears not to affect its function</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* slight dark precipitate appears not to affect its function</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><center>'''[https://2012.igem.org/Team:Cambridge/Protocols#Recipes <u>Back to Protocols and Recipes</u>]'''</center></ins></div></td></tr>
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Emmyft
http://2012.igem.org/wiki/index.php?title=Team:Cambridge/Protocols/CCMB80&diff=292804&oldid=prev
Emmyft at 00:07, 27 October 2012
2012-10-27T00:07:48Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=CCMB80 buffer Recipe=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=CCMB80 buffer Recipe=</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* slight dark precipitate appears not to affect its function</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* slight dark precipitate appears not to affect its function</div></td></tr>
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Emmyft
http://2012.igem.org/wiki/index.php?title=Team:Cambridge/Protocols/CCMB80&diff=80173&oldid=prev
Pdmallaband: /* CCMB80 buffer */
2012-08-23T18:20:39Z
<p><span class="autocomment">CCMB80 buffer</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 18:20, 23 August 2012</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==</del>=CCMB80 buffer=<del class="diffchange diffchange-inline">==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=CCMB80 buffer <ins class="diffchange diffchange-inline">Recipe</ins>=</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">This buffer is used when making chemically competent cells</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 10 mM KOAc pH 7.0 (10 ml of a 1M stock/L or 0.98g/L)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 10 mM KOAc pH 7.0 (10 ml of a 1M stock/L or 0.98g/L)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 80 mM CaCl<sub>2</sub>.2H<sub>2</sub>O (11.8 g/L)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 80 mM CaCl<sub>2</sub>.2H<sub>2</sub>O (11.8 g/L)</div></td></tr>
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Pdmallaband
http://2012.igem.org/wiki/index.php?title=Team:Cambridge/Protocols/CCMB80&diff=80172&oldid=prev
Pdmallaband: Created page with "{{Template:Team:Cambridge/CAM_2012_TEMPLATE_HEAD_DIARY}} ===CCMB80 buffer=== * 10 mM KOAc pH 7.0 (10 ml of a 1M stock/L or 0.98g/L) * 80 mM CaCl<sub>2</sub>.2H<sub>2</sub>O (11...."
2012-08-23T18:20:06Z
<p>Created page with "{{Template:Team:Cambridge/CAM_2012_TEMPLATE_HEAD_DIARY}} ===CCMB80 buffer=== * 10 mM KOAc pH 7.0 (10 ml of a 1M stock/L or 0.98g/L) * 80 mM CaCl<sub>2</sub>.2H<sub>2</sub>O (11...."</p>
<p><b>New page</b></p><div>{{Template:Team:Cambridge/CAM_2012_TEMPLATE_HEAD_DIARY}}<br />
<br />
===CCMB80 buffer===<br />
* 10 mM KOAc pH 7.0 (10 ml of a 1M stock/L or 0.98g/L)<br />
* 80 mM CaCl<sub>2</sub>.2H<sub>2</sub>O (11.8 g/L)<br />
* 20 mM MnCl<sub>2</sub>.4H<sub>2</sub>O (4.0 g/L)<br />
* 10 mM MgCl<sub>2</sub>.6H<sub>2</sub>O (2.0 g/L or 5ml of 2M stock/L)<br />
* 10% glycerol (100 ml/L)<br />
* Fill up with DI water AFTER regulating pH<br />
<br />
* adjust pH DOWN to 6.4 with 0.1N HCl if necessary<br />
** adjusting pH up will precipitate manganese dioxide from Mn containing solutions.<br />
* sterile filter and store at 4&deg;C<br />
* slight dark precipitate appears not to affect its function<br />
<br />
{{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}}</div>
Pdmallaband