Team:Cambridge/Lab book/Week 6

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Week: 3 4 5 6 7

Contents

Monday

Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive.

PCR cycle x35 : 15s Denaturing at 95 C

               45s Annealing
               300s Extension

Tuesday

Wednesday

Thursday

Friday