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Team:Cambridge/Lab book/Week 6
From 2012.igem.org
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===Monday=== | ===Monday=== | ||
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| + | '''[[Team:Cambridge/Protocols/PCRProtocol|PCR of vectors]]''' | ||
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Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive. | Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive. | ||
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The remaining stray product had a slightly tricky secondary structure at the 3' end. It will be run at a series of annealing temperatures in a PCR machine capable of a temperature gradient. | The remaining stray product had a slightly tricky secondary structure at the 3' end. It will be run at a series of annealing temperatures in a PCR machine capable of a temperature gradient. | ||
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| + | '''[[Team:Cambridge/Protocols/TransformationofB.subtilis|Transformation of ''Bacillus'' with riboswitch construct]]''' | ||
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| + | ---- | ||
===Tuesday=== | ===Tuesday=== | ||
Revision as of 14:09, 31 July 2012
| Week: | 3 | 4 | 5 | 6 | 7 |
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Contents |
Monday
Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive.
PCR cycle x35:
- 15s Denaturing at 95 C
- 45s Annealing at 60 C
- 300s Extension at 72 C
The remaining stray product had a slightly tricky secondary structure at the 3' end. It will be run at a series of annealing temperatures in a PCR machine capable of a temperature gradient.
Transformation of Bacillus with riboswitch construct
Tuesday
Wednesday
Thursday
Friday
