Team:Cambridge/Lab book/Week 6

From 2012.igem.org

(Difference between revisions)
(Monday)
(Monday)
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Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive.
Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive.
-
PCR cycle x35 : *15s Denaturing at 95 C
+
PCR cycle x35: *15s Denaturing at 95 C
-
                 *45s Annealing at 60 C
+
                 45s Annealing at 60 C
-
                 *300s Extension at 72 C
+
                 300s Extension at 72 C
===Tuesday===
===Tuesday===

Revision as of 10:45, 30 July 2012

Week: 3 4 5 6 7

Contents

Monday

Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive.

PCR cycle x35: *15s Denaturing at 95 C

               45s Annealing at 60 C
               300s Extension at 72 C

Tuesday

Wednesday

Thursday

Friday