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Team:Cambridge/Lab book/Week 6
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===Monday=== | ===Monday=== | ||
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| + | Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive. | ||
| + | |||
| + | PCR cycle x35 : 15s Denaturing at 95 C | ||
| + | 45s Annealing | ||
| + | 300s Extension | ||
===Tuesday=== | ===Tuesday=== | ||
Revision as of 10:42, 30 July 2012
| Week: | 3 | 4 | 5 | 6 | 7 |
|---|
Contents |
Monday
Not all products were obtained during Friday's PCR. Most of these missing products were large vector backbones. They are being run again, with a much longer extension time of 300s. If that fails, primers will be ordered to split the vectors into manageable chunks, and the PCR reattempted when they arrive.
PCR cycle x35 : 15s Denaturing at 95 C
45s Annealing
300s Extension
Tuesday
Wednesday
Thursday
Friday
