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Team:Cambridge/Diary/Week 13
From 2012.igem.org
| Week: | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 |
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Contents |
Monday
- Ratiometrica: grow O/N cultures for rough IPTG induction tomorrow, ordered primers to convert into biobrick
Tuesday
- Ran PCR for making SPOBB biobrick, gel yielded insufficient results. Redesigned and reordered primers
- Ratiometrica: rough IPTG induction with 8 different concentrations of IPTG, after 3 hours of incubation there was unfortunately no obvious results (i.e. no visible difference between different concentrations/induced and uninduced smaples), will leave them growing overnight and observe again tomorrow; sequencing primers arrived so the plasmid is sent off to sequencing, will have results tomorrow morning; more O/N cultures grown for miniprep to put into Bacillus tomorrow.
Wednesday
- Ratiometrica: Sequencing results came back- good news: CFP is inserted correctly after pSPAC, bad news: more uncertainties as to whether the K143053 and YFP are inserted correctly. Since the cells shows clear YFP under the fluorescent microscope, we cannot really tell what is wrong at the moment, further tests/sequencing primers need to be designed.
Thursday
Friday
Saturday
Sunday
