Team:Cambridge/Diary/Week 13

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Contents

Monday

  • Ratiometrica: grow O/N cultures for rough IPTG induction tomorrow, ordered primers to convert into biobrick

Tuesday

  • Ran PCR for making SPOBB biobrick, gel yielded insufficient results. Redesigned and reordered primers
  • Ratiometrica: rough IPTG induction with 8 different concentrations of IPTG, after 3 hours of incubation there was unfortunately no obvious results (i.e. no visible difference between different concentrations/induced and uninduced smaples), will leave them growing overnight and observe again tomorrow; sequencing primers arrived so the plasmid is sent off to sequencing, will have results tomorrow morning; more O/N cultures grown for miniprep to put into Bacillus tomorrow.

Wednesday

Thursday

Friday

Saturday

Sunday