Team:Cambridge/Diary/Week 13
From 2012.igem.org
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Contents |
Monday
- Ratiometrica: grow O/N cultures for rough IPTG induction tomorrow, ordered primers to convert into biobrick
Tuesday
- Ran PCR for making SPOBB biobrick, gel yielded insufficient results. Redesigned and reordered primers
- Ratiometrica: rough IPTG induction with 8 different concentrations of IPTG, after 3 hours of incubation there was unfortunately no obvious results (i.e. no visible difference between different concentrations/induced and uninduced smaples), will leave them growing overnight and observe again tomorrow; sequencing primers arrived so the plasmid is sent off to sequencing, will have results tomorrow morning; more O/N cultures grown for miniprep to put into Bacillus tomorrow.
Wednesday
Thursday
Friday
Saturday
Sunday