Team:Calgary/Project/Post-Regionals

From 2012.igem.org

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<li><p><b>Further characterized</b> our previously validated <b>magnesium riboswitch kill gene construct</b>.</li></p></ul>
<li><p><b>Further characterized</b> our previously validated <b>magnesium riboswitch kill gene construct</b>.</li></p></ul>
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<p><b>In terms of <FONT COLOR=#159900>FRED</FONT>, we...</b></p>
<p><b>In terms of <FONT COLOR=#159900>FRED</FONT>, we...</b></p>
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Revision as of 09:13, 26 October 2012

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Post-Regional Accomplishments

Our team has had many accomplishments since the regional jamboree!

In our Human Practices project, we...

  • Returned to industry experts to see if we accomplished our goals and what the next steps should be.

  • Characterized the functionality of our previously submitted Prha promoter using GFP fluorescence.

  • Tested an additional complete kill switch device using the Prha promoter with our S7 kill gene.

  • Further characterized our previously validated magnesium riboswitch kill gene construct.


In terms of FRED, we...

  • Characterized an inducible lacz circuit, replacing a faulty existing circuit in the registry containing a frameshift.

  • Characterized a constitutive bglX generator in terms of its electrochemical activity.

  • Characterized and demonstrated how 3 different hydrolase-producing circuits can be used simultaneously in order to create a multiple biosensor.

  • Tested one of our transposon library hits with our electrrchemical reporter, showing that we can use our system to detect toxins electrochemically