http://2012.igem.org/wiki/index.php?title=Team:Calgary/Project/HumanPractices/Interviews&feed=atom&action=historyTeam:Calgary/Project/HumanPractices/Interviews - Revision history2024-03-29T02:06:21ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Calgary/Project/HumanPractices/Interviews&diff=299306&oldid=prevPjwu at 20:28, 15 December 20122012-12-15T20:28:44Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h2<del class="diffchange diffchange-inline">> <u</del>>Initial Interviews<del class="diffchange diffchange-inline"></u> </del></h2></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h2>Initial Interviews</h2></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Talking with Suncor's Christine Daly on Biology in the Oil Sands</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Talking with Suncor's Christine Daly on Biology in the Oil Sands</h3></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h2<del class="diffchange diffchange-inline">><u</del>>Follow-Up Interviews<del class="diffchange diffchange-inline"></u></del></h2></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h2>Follow-Up Interviews</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our second iteration of interviews were conducted once we had a more concrete product built. The purpose of these interviews was to see whether we had successfully addressed the concerns of the first iteration interviews. We also wanted to see whether any new issues with the design existed, which would provide us with potential future directions to take FRED and OSCAR. Kelly Roberge, an independent oil consultant, suggested we investigate ways of dealing with the clay and silt particles in tailings pond water that would enter our bioreactor system. This can be a major problem since these mature fine tailings have a thick consistency that could clog the system.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our second iteration of interviews were conducted once we had a more concrete product built. The purpose of these interviews was to see whether we had successfully addressed the concerns of the first iteration interviews. We also wanted to see whether any new issues with the design existed, which would provide us with potential future directions to take FRED and OSCAR. Kelly Roberge, an independent oil consultant, suggested we investigate ways of dealing with the clay and silt particles in tailings pond water that would enter our bioreactor system. This can be a major problem since these mature fine tailings have a thick consistency that could clog the system.</p></div></td></tr>
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</table>Pjwuhttp://2012.igem.org/wiki/index.php?title=Team:Calgary/Project/HumanPractices/Interviews&diff=298055&oldid=prevPjwu at 03:44, 27 October 20122012-10-27T03:44:11Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We were warned that the oil industry is rather traditional, but as we have seen from our <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Collaborations">OSLI dialogue</a>, it appears that more companies are beginning to see the potential benefit of biology in solving various issues with the oil sands.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We were warned that the oil industry is rather traditional, but as we have seen from our <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Collaborations">OSLI dialogue</a>, it appears that more companies are beginning to see the potential benefit of biology in solving various issues with the oil sands.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Some interesting points for us to explore further included examining whether or not the intermediates of our metabolic pathways were more or less toxic than the starting compounds, and expanding our scope to target other compounds such as polyaromatic hydrocarbons, chlorides, composite tailings, polyphenols, and hydrogen sulfide.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Some interesting points for us to explore further included examining whether or not the intermediates of our metabolic pathways were more or less toxic than the starting compounds, and expanding our scope to target other compounds such as polyaromatic hydrocarbons, chlorides, composite tailings, polyphenols, and hydrogen sulfide.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The interview, in full, has been divided into <del class="diffchange diffchange-inline">four </del>parts. Please click the links below to download them.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The interview, in full, has been divided into <ins class="diffchange diffchange-inline">six </ins>parts. Please click the links below to download them.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="https://static.igem.org/mediawiki/2012/a/aa/UCalgary2012_Zvonko_Burkus_Interview-01.mp3">Part 1</a><br/></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="https://static.igem.org/mediawiki/2012/a/aa/UCalgary2012_Zvonko_Burkus_Interview-01.mp3">Part 1</a><br/></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="https://static.igem.org/mediawiki/2012/b/ba/UCalgary2012_Zvonko_Burkus_Interview-02.mp3">Part 2</a><br/></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="https://static.igem.org/mediawiki/2012/b/ba/UCalgary2012_Zvonko_Burkus_Interview-02.mp3">Part 2</a><br/></p></div></td></tr>
</table>Pjwuhttp://2012.igem.org/wiki/index.php?title=Team:Calgary/Project/HumanPractices/Interviews&diff=298028&oldid=prevPjwu at 03:43, 27 October 20122012-10-27T03:43:22Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We were warned that the oil industry is rather traditional, but as we have seen from our <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Collaborations">OSLI dialogue</a>, it appears that more companies are beginning to see the potential benefit of biology in solving various issues with the oil sands.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We were warned that the oil industry is rather traditional, but as we have seen from our <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Collaborations">OSLI dialogue</a>, it appears that more companies are beginning to see the potential benefit of biology in solving various issues with the oil sands.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Some interesting points for us to explore further included examining whether or not the intermediates of our metabolic pathways were more or less toxic than the starting compounds, and expanding our scope to target other compounds such as polyaromatic hydrocarbons, chlorides, composite tailings, polyphenols, and hydrogen sulfide.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Some interesting points for us to explore further included examining whether or not the intermediates of our metabolic pathways were more or less toxic than the starting compounds, and expanding our scope to target other compounds such as polyaromatic hydrocarbons, chlorides, composite tailings, polyphenols, and hydrogen sulfide.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><del class="diffchange diffchange-inline">This </del>interview <del class="diffchange diffchange-inline">was a phone recording</del>. Please download <del class="diffchange diffchange-inline">it here</del>: </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><ins class="diffchange diffchange-inline">The </ins>interview<ins class="diffchange diffchange-inline">, in full, has been divided into four parts</ins>. Please <ins class="diffchange diffchange-inline">click the links below to </ins>download <ins class="diffchange diffchange-inline">them.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p><a href="https</ins>:<ins class="diffchange diffchange-inline">//static.igem.org/mediawiki/2012/a/aa/UCalgary2012_Zvonko_Burkus_Interview-01.mp3">Part 1</a><br/></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p><a href="https://static.igem.org/mediawiki/2012/b/ba/UCalgary2012_Zvonko_Burkus_Interview-02.mp3">Part 2</a><br/></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p><a href="https://static.igem.org/mediawiki/2012/0/0e/UCalgary2012_Zvonko_Burkus_Interview-03.mp3">Part 3</a><br/></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p><a href="https://static.igem.org/mediawiki/2012/b/b8/UCalgary2012_Zvonko_Burkus_Interview-04.mp3">Part 4</a><br/></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p><a href="https://static.igem.org/mediawiki/2012/c/c1/UCalgary2012_Zvonko_Burkus_Interview-05.mp3">Part 5</a><br/></p></ins></div></td></tr>
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</table>Pjwuhttp://2012.igem.org/wiki/index.php?title=Team:Calgary/Project/HumanPractices/Interviews&diff=297561&oldid=prevJeffaddison89 at 03:28, 27 October 20122012-10-27T03:28:49Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Zvonko Burkus from Alberta Environment Discusses the Project in Detail</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Zvonko Burkus from Alberta Environment Discusses the Project in Detail</h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Zvonko is a process water and policy specialist for Alberta Environment. Zvonko was happy to discuss with us at length about this project. Notably, there were concerns about the use of both <a class="green" href="https://2012.igem.org/Team:Calgary/Project/FRED">FRED </a> and <a class="blue" href="https://2012.igem.org/Team:Calgary/Project/OSCAR">OSCAR</a> in active tailings ponds, since naphthenic acids are known surfactants which help with bitumen detachment from the sand particles. FRED was seen as something which could be more useful in a live-monitoring system, since currently there are no such systems for organic compounds, which is a possible direction for us.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Zvonko is a process water and policy specialist for Alberta Environment. Zvonko was happy to discuss with us at length about this project. Notably, there were concerns about the use of both <a class="green" href="https://2012.igem.org/Team:Calgary/Project/FRED">FRED </a> and <a class="blue" href="https://2012.igem.org/Team:Calgary/Project/OSCAR">OSCAR</a> in active tailings ponds, since naphthenic acids are known surfactants which help with bitumen detachment from the sand particles. FRED was seen as something which could be more useful in a live-monitoring system, since currently there are no such systems for organic compounds, which is a possible direction for us.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>We were warned that the oil industry is rather traditional, but as we have seen from our <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/<del class="diffchange diffchange-inline">Collaboration</del>">OSLI dialogue</a>, it appears that more companies are beginning to see the potential benefit of biology in solving various issues with the oil sands.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>We were warned that the oil industry is rather traditional, but as we have seen from our <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/<ins class="diffchange diffchange-inline">Collaborations</ins>">OSLI dialogue</a>, it appears that more companies are beginning to see the potential benefit of biology in solving various issues with the oil sands.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Some interesting points for us to explore further included examining whether or not the intermediates of our metabolic pathways were more or less toxic than the starting compounds, and expanding our scope to target other compounds such as polyaromatic hydrocarbons, chlorides, composite tailings, polyphenols, and hydrogen sulfide.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Some interesting points for us to explore further included examining whether or not the intermediates of our metabolic pathways were more or less toxic than the starting compounds, and expanding our scope to target other compounds such as polyaromatic hydrocarbons, chlorides, composite tailings, polyphenols, and hydrogen sulfide.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This interview was a phone recording. Please download it here: </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This interview was a phone recording. Please download it here: </p></div></td></tr>
</table>Jeffaddison89http://2012.igem.org/wiki/index.php?title=Team:Calgary/Project/HumanPractices/Interviews&diff=297526&oldid=prevJeffaddison89 at 03:27, 27 October 20122012-10-27T03:27:50Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Zvonko Burkus from Alberta Environment Discusses the Project in Detail</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Zvonko Burkus from Alberta Environment Discusses the Project in Detail</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Zvonko is a process water and policy specialist for Alberta Environment. Zvonko was happy to discuss with us at length about this project. Notably, there were concerns about the use of both FRED and OSCAR in active tailings ponds, since naphthenic acids are known surfactants which help with bitumen detachment from the sand particles. FRED was seen as something which could be more useful in a live-monitoring system, since currently there are no such systems for organic compounds, which is a possible direction for us.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Zvonko is a process water and policy specialist for Alberta Environment. Zvonko was happy to discuss with us at length about this project. Notably, there were concerns about the use of both <ins class="diffchange diffchange-inline"><a class="green" href="https://2012.igem.org/Team:Calgary/Project/</ins>FRED<ins class="diffchange diffchange-inline">">FRED </a> </ins>and <ins class="diffchange diffchange-inline"><a class="blue" href="https://2012.igem.org/Team:Calgary/Project/OSCAR"></ins>OSCAR<ins class="diffchange diffchange-inline"></a> </ins>in active tailings ponds, since naphthenic acids are known surfactants which help with bitumen detachment from the sand particles. FRED was seen as something which could be more useful in a live-monitoring system, since currently there are no such systems for organic compounds, which is a possible direction for us.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We were warned that the oil industry is rather traditional, but as we have seen from our <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Collaboration">OSLI dialogue</a>, it appears that more companies are beginning to see the potential benefit of biology in solving various issues with the oil sands.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We were warned that the oil industry is rather traditional, but as we have seen from our <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Collaboration">OSLI dialogue</a>, it appears that more companies are beginning to see the potential benefit of biology in solving various issues with the oil sands.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Some interesting points for us to explore further included examining whether or not the intermediates of our metabolic pathways were more or less toxic than the starting compounds, and expanding our scope to target other compounds such as polyaromatic hydrocarbons, chlorides, composite tailings, polyphenols, and hydrogen sulfide.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Some interesting points for us to explore further included examining whether or not the intermediates of our metabolic pathways were more or less toxic than the starting compounds, and expanding our scope to target other compounds such as polyaromatic hydrocarbons, chlorides, composite tailings, polyphenols, and hydrogen sulfide.</p></div></td></tr>
</table>Jeffaddison89http://2012.igem.org/wiki/index.php?title=Team:Calgary/Project/HumanPractices/Interviews&diff=297465&oldid=prevMyarcell at 03:25, 27 October 20122012-10-27T03:25:53Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>William Sawchuk, a reservoir engineer at Arc resources, agreed to talk with us about the main parts of our project. This interview confirmed that biological methods, and specifically our project, are definite possibilities of remediation in the oil sands if they can prove to be faster and less harmful than current methods. One concern that Mr. Sawchuk brought up was that there needs to be extra safety features put in place to avoid posing danger to the environment. This again, serves to further validate the approach that we took to safety, designing both <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Design"> structural and genetic killswitch devices</a>. In the later part of our project, we have also been trying to work on establishing a <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy">glycine auxotrophic killswitch</a> to add yet another layer of safety which we feel is necessary. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>William Sawchuk, a reservoir engineer at Arc resources, agreed to talk with us about the main parts of our project. This interview confirmed that biological methods, and specifically our project, are definite possibilities of remediation in the oil sands if they can prove to be faster and less harmful than current methods. One concern that Mr. Sawchuk brought up was that there needs to be extra safety features put in place to avoid posing danger to the environment. This again, serves to further validate the approach that we took to safety, designing both <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Design"> structural and genetic killswitch devices</a>. In the later part of our project, we have also been trying to work on establishing a <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy">glycine auxotrophic killswitch</a> to add yet another layer of safety which we feel is necessary. </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Similar to Mr. Roberge, another thing Mr. Sawchuk brought up was scale-up. Specifically, he talked about feasibility and how much a scale-up of the project would cost and if this is less expensive than the current remediation methods. To this end, we have been experimenting with getting our bioreactor to work and have performed an initial validation assay to demonstrate that we can use it in conjunction with our belt skimmer to produce and harvest hydrocarbons, which can be found on our <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy">Synergy</a> page. The next step is to scale-up further! <del class="diffchange diffchange-inline">The </del>exact <del class="diffchange diffchange-inline">cost </del>is a <del class="diffchange diffchange-inline">bit </del>tricky. Since the conversion of toxins in the tailings ponds into useful hydrocarbons is a relatively novel idea, it is somewhat difficult to analyze what the cost of a scale-up would be at this point. This is an extremely important future direction for us however.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Similar to Mr. Roberge, another thing Mr. Sawchuk brought up was scale-up. Specifically, he talked about feasibility and how much a scale-up of the project would cost and if this is less expensive than the current remediation methods. To this end, we have been experimenting with getting our bioreactor to work and have performed an initial validation assay to demonstrate that we can use it in conjunction with our belt skimmer to produce and harvest hydrocarbons, which can be found on our <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy">Synergy</a> page. The next step is to scale-up further! <ins class="diffchange diffchange-inline">Calculating </ins>exact <ins class="diffchange diffchange-inline">costs </ins>is a tricky. Since the conversion of toxins in the tailings ponds into useful hydrocarbons is a relatively novel idea, it is somewhat difficult to analyze what the cost of a scale-up would be at this point. This is an extremely important future direction for us however.</p></div></td></tr>
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</table>Myarcellhttp://2012.igem.org/wiki/index.php?title=Team:Calgary/Project/HumanPractices/Interviews&diff=297329&oldid=prevMyarcell at 03:21, 27 October 20122012-10-27T03:21:17Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>William Sawchuk, of ARC Resources</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>William Sawchuk, of ARC Resources</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>William Sawchuk, a reservoir engineer at Arc resources, agreed to talk with us about the main parts of our project. This interview confirmed that biological methods, and specifically our project, are definite possibilities of remediation in the oil sands if they can prove to be faster and less harmful than current methods. One concern that <del class="diffchange diffchange-inline">William </del>brought up was that there needs to be extra safety <del class="diffchange diffchange-inline">factors </del>put in place to avoid posing danger to the environment. This again, serves to further validate the approach that we took to safety, designing both <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Design"> structural and genetic killswitch devices</a>. In the later part of our project, we have also been trying to work on establishing a <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy">glycine auxotrophic killswitch</a> to add yet another layer of safety which we feel is necessary. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>William Sawchuk, a reservoir engineer at Arc resources, agreed to talk with us about the main parts of our project. This interview confirmed that biological methods, and specifically our project, are definite possibilities of remediation in the oil sands if they can prove to be faster and less harmful than current methods. One concern that <ins class="diffchange diffchange-inline">Mr. Sawchuk </ins>brought up was that there needs to be extra safety <ins class="diffchange diffchange-inline">features </ins>put in place to avoid posing danger to the environment. This again, serves to further validate the approach that we took to safety, designing both <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Design"> structural and genetic killswitch devices</a>. In the later part of our project, we have also been trying to work on establishing a <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy">glycine auxotrophic killswitch</a> to add yet another layer of safety which we feel is necessary. </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Similar to Mr. Roberge, another thing Mr. Sawchuk brought up was scale-up. Specifically, he talked about feasibility and <del class="diffchange diffchange-inline">cost </del>a scale-up of the project would cost and if this is less expensive than the current remediation methods. To this end, <del class="diffchange diffchange-inline">we’ve </del>been experimenting with <del class="diffchange diffchange-inline">starting to get </del>our bioreactor <del class="diffchange diffchange-inline">working </del>and have performed an initial validation assay that we can use it in conjunction with our belt skimmer to produce and harvest hydrocarbons, which can be found on our <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy">Synergy</a> page. The next step is to scale-up further! The exact cost is a bit tricky. Since the conversion of toxins in the tailings ponds into useful hydrocarbons is a relatively novel idea, it is somewhat difficult to analyze what the cost of a scale-up would be at this point. This is an extremely important future direction for us however.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Similar to Mr. Roberge, another thing Mr. Sawchuk brought up was scale-up. Specifically, he talked about feasibility and <ins class="diffchange diffchange-inline">how much </ins>a scale-up of the project would cost and if this is less expensive than the current remediation methods. To this end, <ins class="diffchange diffchange-inline">we have </ins>been experimenting with <ins class="diffchange diffchange-inline">getting </ins>our bioreactor <ins class="diffchange diffchange-inline">to work </ins>and have performed an initial validation assay <ins class="diffchange diffchange-inline">to demonstrate </ins>that we can use it in conjunction with our belt skimmer to produce and harvest hydrocarbons, which can be found on our <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy">Synergy</a> page. The next step is to scale-up further! The exact cost is a bit tricky. Since the conversion of toxins in the tailings ponds into useful hydrocarbons is a relatively novel idea, it is somewhat difficult to analyze what the cost of a scale-up would be at this point. This is an extremely important future direction for us however.</p></div></td></tr>
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</table>Myarcellhttp://2012.igem.org/wiki/index.php?title=Team:Calgary/Project/HumanPractices/Interviews&diff=297203&oldid=prevMyarcell at 03:16, 27 October 20122012-10-27T03:16:58Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Kelly Roberge, of K. Roberge Consulting Ltd. Discussing Bioreactor Improvements</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Kelly Roberge, of K. Roberge Consulting Ltd. Discussing Bioreactor Improvements</h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We spoke to Kelly Roberge of K. Roberge Consulting Ltd. who is an independent consultant for the oil sands focusing on mature fine tailings (MFT). He mentioned that in the past 4 years, there has been an increase in looking at biological techniques in the oil sands for remediation, both in understanding natively present microbial life as well as introducing engineered systems.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We spoke to Kelly Roberge of K. Roberge Consulting Ltd. who is an independent consultant for the oil sands focusing on mature fine tailings (MFT). He mentioned that in the past 4 years, there has been an increase in looking at biological techniques in the oil sands for remediation, both in understanding natively present microbial life as well as introducing engineered systems.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p> The major concerns that he had with our present design were issues with scale-up. These were things such as the amount of toxins that would have to be processed <del class="diffchange diffchange-inline">need </del>to provide constant generation of product, residence time in the bioreactor, as well as the ability for our system to be scaled up to an industrial size. Though we still have much research to do <del class="diffchange diffchange-inline">towards </del>this goal of reaching industrial capacity, we did a model scale-up experiment of OSCAR by growing the PetroBrick containing <i>E. coli</i> in our model bioreactor system. The results of this experiment can be found on our <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy">Synergy</a> page.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p> The major concerns that he had with our present design were issues with scale-up. These were things such as the amount of toxins that would have to be processed to provide constant generation of product, residence time in the bioreactor, as well as the ability for our system to be scaled up to an industrial size. Though we still have much research to do <ins class="diffchange diffchange-inline">toward </ins>this goal of reaching industrial capacity, we did a model scale-up experiment of OSCAR by growing the PetroBrick containing <i>E. coli</i> in our model bioreactor system. The results of this experiment can be found on our <a class="purple" href="https://2012.igem.org/Team:Calgary/Project/Synergy">Synergy</a> page.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>In addition, there was a concern raised with the composition of the tailings themselves, due to the mature fine tailings sludge (MFT). In the future we <del class="diffchange diffchange-inline">will have to </del>look at <del class="diffchange diffchange-inline">the limitations in terms of </del>the capacity of OSCAR to deal with these MFT components. Some suggestions that were made would be to utilize OSCAR in parallel with MFT settling techniques or with runoff water from the tailings drying processes. The sensitivity of our system to <del class="diffchange diffchange-inline">this grime </del>and to bitumen would also have to be evaluated and made compatible with the substrates we will be adding in to the system.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>In addition, there was a concern raised with the composition of the tailings themselves, due to the mature fine tailings sludge (MFT). In the future we <ins class="diffchange diffchange-inline">should </ins>look at <ins class="diffchange diffchange-inline">addressing </ins>the capacity of OSCAR to deal with these MFT components. Some suggestions that were made would be to utilize OSCAR in parallel with MFT settling techniques or with runoff water from the tailings drying processes. The sensitivity of our system to <ins class="diffchange diffchange-inline">these fine tailings </ins>and to bitumen would also have to be evaluated and made compatible with the substrates we will be adding in to the system.</p></div></td></tr>
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</table>Myarcellhttp://2012.igem.org/wiki/index.php?title=Team:Calgary/Project/HumanPractices/Interviews&diff=297094&oldid=prevPjwu at 03:12, 27 October 20122012-10-27T03:12:58Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Gordon Lambert is the VP Sustainable Development at Suncor Energy Inc. We asked him whether or not the oil sands industry would find technology such as this useful. There was a very positive response. The Oil Sands Leadership Initiative is very keen on searching for any solutions to tackle the tailings ponds, which are considered to be one of the biggest issues in the oil sands currently. <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Collaborations">OSLI</a> is collaborating with organizations that run competitions globally for oil sands solutions and other bodies such the Canada's Oil Sands Innovation Alliance (COSIA). Similar to Kelly Roberge's comment, mature fine tailings can be dried and solidified, but in turn it liberates water from the clay and sand. This water cannot be used for any industrial purposes until it is detoxified. Ideally, this water can be detoxified sufficiently to be returned as tailings pond surface water and become reusable in the bitumen extraction process.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Gordon Lambert is the VP Sustainable Development at Suncor Energy Inc. We asked him whether or not the oil sands industry would find technology such as this useful. There was a very positive response. The Oil Sands Leadership Initiative is very keen on searching for any solutions to tackle the tailings ponds, which are considered to be one of the biggest issues in the oil sands currently. <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Collaborations">OSLI</a> is collaborating with organizations that run competitions globally for oil sands solutions and other bodies such the Canada's Oil Sands Innovation Alliance (COSIA). Similar to Kelly Roberge's comment, mature fine tailings can be dried and solidified, but in turn it liberates water from the clay and sand. This water cannot be used for any industrial purposes until it is detoxified. Ideally, this water can be detoxified sufficiently to be returned as tailings pond surface water and become reusable in the bitumen extraction process.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In order to deploy our biosensor and bioreactor system, it was suggested that we look into various regulatory boards within Alberta such as Alberta Environment and the Energy Resources Conservation Board (ERCB) to attempt to obtain permits to begin attempting pilot programs. <a href="https://2012.igem.org/Team:Calgary/Project/Synergy">Scale up</a> of the bioreactor is also a major consideration in order for us to push it off the bench and into the field.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>In order to deploy our biosensor and bioreactor system, it was suggested that we look into various regulatory boards within Alberta such as Alberta Environment and the Energy Resources Conservation Board (ERCB) to attempt to obtain permits to begin attempting pilot programs. <a href="https://2012.igem.org/Team:Calgary/Project/Synergy">Scale up</a> of the bioreactor is also a major consideration in order for us to push it off the bench and into the field.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The full interview can be found below<del class="diffchange diffchange-inline">. If it does not load, <a href="http://www.youtube.com/watch?v=7KbEjQVUsFA">click here</a></del>.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The full interview can be found below.</p></div></td></tr>
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</table>Pjwuhttp://2012.igem.org/wiki/index.php?title=Team:Calgary/Project/HumanPractices/Interviews&diff=296791&oldid=prevPjwu at 03:02, 27 October 20122012-10-27T03:02:32Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div></<del class="diffchange diffchange-inline">div</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><h3>Zvonko Burkus from Alberta Environment Discusses the Project in Detail</h3></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>Zvonko is a process water and policy specialist for Alberta Environment. Zvonko was happy to discuss with us at length about this project. Notably, there were concerns about the use of both FRED and OSCAR in active tailings ponds, since naphthenic acids are known surfactants which help with bitumen detachment from the sand particles. FRED was seen as something which could be more useful in a live-monitoring system, since currently there are no such systems for organic compounds, which is a possible direction for us.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>We were warned that the oil industry is rather traditional, but as we have seen from our <a href="https://2012.igem.org/Team:Calgary/Project/HumanPractices/Collaboration">OSLI dialogue</a>, it appears that more companies are beginning to see the potential benefit of biology in solving various issues with the oil sands.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>Some interesting points for us to explore further included examining whether or not the intermediates of our metabolic pathways were more or less toxic than the starting compounds, and expanding our scope to target other compounds such as polyaromatic hydrocarbons, chlorides, composite tailings, polyphenols, and hydrogen sulfide.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>This interview was a phone recording. Please download it here: </ins></<ins class="diffchange diffchange-inline">p</ins>></div></td></tr>
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