Team:British Columbia/Notebook

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==June 5th==
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Grew cell cultures of EPI 300, BL21, and DH5α, with plasmid PIJ 790 in them, harvested, and stored at -80 freezer.  
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<div id="sponsormap"><img align="left" src="https://static.igem.org/mediawiki/2012/a/a0/Ubcigemnotebookmenu.jpg" usemap="#sponsormap" alt="UBC iGEM 2012 notebook"> </div>
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-Ruichen
 
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==June 6th==
 
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Learned how to electrophorese cells with biobricks (1-7B, 2-17F, 2-14N, 2-9B, 3-13M, 3-14E, 4-3I, 5-1A, 5-12O), and plated them on plates to grow colonies.
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</div><div id=note><p align=center><font face=arial narrow size=5><b>Welcome to our Notebook!</b></font></p><font face=arial narrow>
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Our Notebook is sorted by sub-project. Please use the menu to navigate through our Notebook entries and follow our progress over the year.</br> </br>
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Tested KAN plates with control.
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<b>UBC iGEM Life</b> chronicles the conferences, socials and life outside-of-the-lab of our team.</br></br>
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<b>Biobrick Construction</b> describes how our team build our parts and improved existing parts from the Parts Registry.</br></br>
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-Ruichen
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<b>Bio-Desulfurization</b> recounts the steps our team took to distribute and assess the efficiency of the DSZ bio-desulfurization pathway in a microbial consortia.</br></br>
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<b>Consortia Dynamics</b> details our team's efforts in measuring population ratios within microbial consortia based on fluorescence readings.</br></br>
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==June 7th==
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<b>Consortia Tunability</b> elucidates our team's progress in creating easily tunable microbial consortia based on interdependent auxotrophies.
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Retreated the plates previously spread, KAN plates worked and most of them grew into colonies or covered plates completely. (advised to spread the plates with 50µl recovered cells instead 100µl in the future for better colony identification).  
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Made more antibiotics of Amp (10µg/ml) and Chlor (34µg/ml), and more agar plates of (Amp and Chlor) (half bagful respectively).
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Electrophoresed 1-1N, 2-2K, 1-5P, 2-21O using DH5α, and plated them.  
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-Ruichen
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In the afternoon, we (Ruichen, Mehul, Grace) made many more K12 competent cells, which we put into storage. (The protocol is now on the wiki.) There was also talk from Jacob and John about buying $0.50 worth of gasoline/diesel from the nearest gas station to test solvent resistance of some of our cells. I left before that happened, though. (Would have been fun to see the attendant's reaction!)
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==June 8th==
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Tried to make more EPI300 cells, though no obvious growth even after hours for the second time, and decided to leave it over night to see if there are any changes.
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Electrophoresed K12 with PIJ 790 plasmid, and plated them on Chlor plates with control plates to examine the plates.  
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- Ruichen
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==June 9th==
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The EPI300 did grow!
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- Ruichen
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Bought $1 of 91 octane (no ethanol) gas from the Shell on 10th. It's in a red jerrycan in the flammables cabinet near our bench.
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[[User:JohnHenry|JohnHenry]] 14:31, 9 June 2012 (CDT)
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Latest revision as of 01:42, 4 October 2012

British Columbia - 2012.igem.org
UBC iGEM 2012 notebook

Welcome to our Notebook!

Our Notebook is sorted by sub-project. Please use the menu to navigate through our Notebook entries and follow our progress over the year.

UBC iGEM Life chronicles the conferences, socials and life outside-of-the-lab of our team.

Biobrick Construction describes how our team build our parts and improved existing parts from the Parts Registry.

Bio-Desulfurization recounts the steps our team took to distribute and assess the efficiency of the DSZ bio-desulfurization pathway in a microbial consortia.

Consortia Dynamics details our team's efforts in measuring population ratios within microbial consortia based on fluorescence readings.

Consortia Tunability elucidates our team's progress in creating easily tunable microbial consortia based on interdependent auxotrophies.

Retrieved from "http://2012.igem.org/Team:British_Columbia/Notebook"