http://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Modell&feed=atom&action=historyTeam:Bielefeld-Germany/Modell - Revision history2024-03-29T07:33:45ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Modell&diff=299117&oldid=prevSwiebe: /* Extend the model with sewage plant data */2012-11-18T23:29:55Z<p><span class="autocomment">Extend the model with sewage plant data</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_Schlossholte_Daten.jpg|600px|thumb|left|'''Figure 2: '''Date taken from the sewage plant located in Schloss Holte. Temperature minimum: 8.1 °C, maximum: 20,8 °C and average temperature 14.4 °C; pH minimum: 6.6, maximum: 7.2 and average 6.9; flow minimum: 39.2 L s<sup>-1</sup>, maximum 232.8 L s<sup>-1</sup> and average 76.8 L s<sup>-1</sup>;]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_Schlossholte_Daten.jpg|600px|thumb|left|'''Figure 2: '''Date taken from the sewage plant located in Schloss Holte. Temperature minimum: 8.1 °C, maximum: 20,8 °C and average temperature 14.4 °C; pH minimum: 6.6, maximum: 7.2 and average 6.9; flow minimum: 39.2 L s<sup>-1</sup>, maximum 232.8 L s<sup>-1</sup> and average 76.8 L s<sup>-1</sup>;]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The sewage plant in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and flow rate. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to degrade 80 % of the substrates. This will be the <del class="diffchange diffchange-inline">residence </del>time. Combined with the actual flow rate we can determine the reactor size. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A model of the required enzyme amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and flow rate.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The sewage plant in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and flow rate. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to degrade 80 % of the substrates. This will be the <ins class="diffchange diffchange-inline">dwell </ins>time. Combined with the actual flow rate we can determine the reactor size. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A model of the required enzyme amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and flow rate.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_3D.jpg|600px|thumb|left|'''Figure 3: '''The required enzyme amount (z axis) for a sewage plant is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). In the optimal case the needed enzyme amount is 29 g laccase to degrade 80 % of ABTS. In the worst case 350 g laccase will be needed.]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_3D.jpg|600px|thumb|left|'''Figure 3: '''The required enzyme amount (z axis) for a sewage plant is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). In the optimal case the needed enzyme amount is 29 g laccase to degrade 80 % of ABTS. In the worst case 350 g laccase will be needed.]]</div></td></tr>
</table>Swiebehttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Modell&diff=286547&oldid=prevSwiebe: /* Extend the model with sewage plant data */2012-10-26T16:44:45Z<p><span class="autocomment">Extend the model with sewage plant data</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Extend the model with sewage plant data==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Extend the model with sewage plant data==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_Schlossholte_Daten.jpg|600px|thumb|left|Date taken from the sewage plant located in Schloss Holte. Temperature minimum: 8.1 °C, maximum: 20,8 °C and average temperature 14.4 °C; pH minimum: 6.6, maximum: 7.2 and average 6.9; flow minimum: 39.2 L s<sup>-1</sup>, maximum 232.8 L s<sup>-1</sup> and average 76.8 L s<sup>-1</sup>;]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_Schlossholte_Daten.jpg|600px|thumb|left|<ins class="diffchange diffchange-inline">'''Figure 2: '''</ins>Date taken from the sewage plant located in Schloss Holte. Temperature minimum: 8.1 °C, maximum: 20,8 °C and average temperature 14.4 °C; pH minimum: 6.6, maximum: 7.2 and average 6.9; flow minimum: 39.2 L s<sup>-1</sup>, maximum 232.8 L s<sup>-1</sup> and average 76.8 L s<sup>-1</sup>;]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The sewage plant in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and flow rate. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to degrade 80 % of the substrates. This will be the residence time. Combined with the actual flow rate we can determine the reactor size. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A model of the required enzyme amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and flow rate.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The sewage plant in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and flow rate. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to degrade 80 % of the substrates. This will be the residence time. Combined with the actual flow rate we can determine the reactor size. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A model of the required enzyme amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and flow rate.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_3D.jpg|600px|thumb|left|The required enzyme amount (z axis) for a sewage plant is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). In the optimal case the needed enzyme amount is 29 g laccase to degrade 80 % of ABTS. In the worst case 350 g laccase will be needed.]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_3D.jpg|600px|thumb|left|<ins class="diffchange diffchange-inline">'''Figure 3: '''</ins>The required enzyme amount (z axis) for a sewage plant is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). In the optimal case the needed enzyme amount is 29 g laccase to degrade 80 % of ABTS. In the worst case 350 g laccase will be needed.]]</div></td></tr>
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</table>Swiebehttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Modell&diff=286526&oldid=prevSwiebe: /* The reaction */2012-10-26T16:43:32Z<p><span class="autocomment">The reaction</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_model.jpg|center|800px|thumb|The model with different K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values. The span differs from 0.07 seconds to 1500 seconds to degrade all ABTS.]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_model.jpg|center|800px|thumb|<ins class="diffchange diffchange-inline">'''Figure 1: '''</ins>The model with different K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values. The span differs from 0.07 seconds to 1500 seconds to degrade all ABTS.]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We use K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values from 11 to 643500 that result in degradation time points from 0.07s to 1500s. Furthermore we try to integrate different temperatures to our model. In a span from 10°C to 30°C and a K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value of 1, the degradation differs from 40 s to 100s.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We use K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values from 11 to 643500 that result in degradation time points from 0.07s to 1500s. Furthermore we try to integrate different temperatures to our model. In a span from 10°C to 30°C and a K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value of 1, the degradation differs from 40 s to 100s.</div></td></tr>
</table>Swiebehttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Modell&diff=286380&oldid=prevSwiebe: /* Model of a fixed-bed reactor */2012-10-26T16:34:21Z<p><span class="autocomment">Model of a fixed-bed reactor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Model of a fixed-bed reactor==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Model of a fixed-bed reactor==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In our project we plan to construct a fixed<del class="diffchange diffchange-inline">-</del>bed <del class="diffchange diffchange-inline">reactor</del>, where immobilized laccases degrade synthetic estrogen and other harmful substances. As a small selection we plan to characterize our different laccases for three estrogens, three analgesics, four PAHs, one insecticide and three possible redox mediators.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In our project we plan to construct a fixed bed <ins class="diffchange diffchange-inline">flocculation filtration tank</ins>, where immobilized laccases degrade synthetic estrogen and other harmful substances. As a small selection we plan to characterize our different laccases for three estrogens, three analgesics, four PAHs, one insecticide and three possible redox mediators.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>If we could model the degradation of one substrate by one laccase, we could easily replace the specific K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> quotient of other laccases and the amounts of the other substrates.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>If we could model the degradation of one substrate by one laccase, we could easily replace the specific K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> quotient of other laccases and the amounts of the other substrates.</div></td></tr>
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</table>Swiebehttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Modell&diff=273022&oldid=prevIsahu: /* Extend the model with sewage plant data */2012-10-21T21:50:13Z<p><span class="autocomment">Extend the model with sewage plant data</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The sewage plant in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and flow rate. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to degrade 80 % of the substrates. This will be the residence time. Combined with the actual flow rate we can determine the reactor size. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A model of the required enzyme amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and flow rate.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The sewage plant in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and flow rate. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to degrade 80 % of the substrates. This will be the residence time. Combined with the actual flow rate we can determine the reactor size. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A model of the required enzyme amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and flow rate.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_3D.jpg|600px|thumb|left|The required enzyme amount (z axis) for a sewage plant is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). <del class="diffchange diffchange-inline">Optimal we will need 40 Kg </del>laccase. In the worst case <del class="diffchange diffchange-inline">we </del>will <del class="diffchange diffchange-inline">need 140 Kg</del>. ]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_3D.jpg|600px|thumb|left|The required enzyme amount (z axis) for a sewage plant is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). <ins class="diffchange diffchange-inline">In the optimal case the needed enzyme amount is 29 g </ins>laccase <ins class="diffchange diffchange-inline">to degrade 80 % of ABTS</ins>. In the worst case <ins class="diffchange diffchange-inline">350 g laccase </ins>will <ins class="diffchange diffchange-inline">be needed</ins>.]]</div></td></tr>
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</table>Isahuhttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Modell&diff=273021&oldid=prevIsahu: /* Extend the model with sewage plant data */2012-10-21T21:45:42Z<p><span class="autocomment">Extend the model with sewage plant data</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The sewage plant in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and flow rate. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to degrade 80 % of the substrates. This will be the residence time. Combined with the actual flow rate we can determine the reactor size. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A model of the required enzyme amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and flow rate.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The sewage plant in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and flow rate. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to degrade 80 % of the substrates. This will be the residence time. Combined with the actual flow rate we can determine the reactor size. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A model of the required enzyme amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and flow rate.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:<del class="diffchange diffchange-inline">Bielefeld2012_3d</del>.jpg|600px|thumb|left|The required enzyme amount (z axis) for a sewage plant is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). Optimal we will need 40 Kg laccase. In the worst case we will need 140 Kg. ]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:<ins class="diffchange diffchange-inline">Bielefeld2012_3D</ins>.jpg|600px|thumb|left|The required enzyme amount (z axis) for a sewage plant is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). Optimal we will need 40 Kg laccase. In the worst case we will need 140 Kg. ]]</div></td></tr>
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</table>Isahuhttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Modell&diff=271736&oldid=prevIsahu: /* Extend the model with sewage plant data */2012-10-20T13:24:02Z<p><span class="autocomment">Extend the model with sewage plant data</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Extend the model with sewage plant data==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Extend the model with sewage plant data==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_Schlossholte_Daten.jpg|600px|thumb|left|Date taken from the sewage plant located in Schloss Holte. Temperature minimum: 8.1 °C, maximum: 20,8 °C and average temperature 14.4 °C; pH minimum: 6.6, maximum: 7.2 and average 6.9; flow minimum: 39.2 L s<sup>-1</sup>, maximum 232.8 L s<sup>-1</sup> and average 76.8 L s<sup>-1</sup>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_Schlossholte_Daten.jpg|600px|thumb|left|Date taken from the sewage plant located in Schloss Holte. Temperature minimum: 8.1 °C, maximum: 20,8 °C and average temperature 14.4 °C; pH minimum: 6.6, maximum: 7.2 and average 6.9; flow minimum: 39.2 L s<sup>-1</sup>, maximum 232.8 L s<sup>-1</sup> and average 76.8 L s<sup>-1</sup><ins class="diffchange diffchange-inline">;</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The sewage plant in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and flow rate. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to degrade 80 % of the substrates. This will be the residence time. Combined with the actual flow rate we can determine the reactor size. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A <del class="diffchange diffchange-inline">3D </del>model of the required enzyme amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and flow rate.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The sewage plant in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and flow rate. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to degrade 80 % of the substrates. This will be the residence time. Combined with the actual flow rate we can determine the reactor size. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A model of the required enzyme amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and flow rate.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_3d.jpg|600px|thumb|left|The required enzyme amount (z axis) for a sewage plant is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). Optimal we will need 40 Kg laccase. In the worst case we will need 140 Kg. ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_3d.jpg|600px|thumb|left|The required enzyme amount (z axis) for a sewage plant is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). Optimal we will need 40 Kg laccase. In the worst case we will need 140 Kg. ]]</div></td></tr>
</table>Isahuhttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Modell&diff=271734&oldid=prevIsahu: /* The reaction */2012-10-20T13:22:35Z<p><span class="autocomment">The reaction</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This transformation of the Michaelis Menten kinetic can be found in "Stryer biochemie". The resulting formula is suitable for very low substrate concentrations. In this case we can estimate degradation of initial substrate [https://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Project/Background/Concentrations concentrations] below 0.1 µg L<sup>-1</sup>.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This transformation of the Michaelis Menten kinetic can be found in "Stryer biochemie". The resulting formula is suitable for very low substrate concentrations. In this case we can estimate degradation of initial substrate [https://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Project/Background/Concentrations concentrations] below 0.1 µg L<sup>-1</sup>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Because <del class="diffchange diffchange-inline">we can not find </del>any information about the K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> quotient for the degradation of [https://2012.igem.org/Team:Bielefeld-Germany/Project/Background#Estradiol: estradiol] in the enzyme database [http://www.brenda-enzymes.info Brenda] <del class="diffchange diffchange-inline">we decided to use </del>the K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> quotients for the <del class="diffchange diffchange-inline">degradation </del>of [http://www.sigmaaldrich.com/catalog/product/sigma/a1888?lang=de&region=DE ABTS], a redox mediator of the laccase. <del class="diffchange diffchange-inline">We use </del>ABTS in this case only as placeholder, until <del class="diffchange diffchange-inline">we get </del>suitable data for the substrates. In the following picture <del class="diffchange diffchange-inline">we show </del>a few possible reactions of our laccase. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Because <ins class="diffchange diffchange-inline">there isn't </ins>any information about the K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> quotient for the degradation of [https://2012.igem.org/Team:Bielefeld-Germany/Project/Background#Estradiol: estradiol] in the enzyme database [http://www.brenda-enzymes.info Brenda] the K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> quotients <ins class="diffchange diffchange-inline">were used </ins>for <ins class="diffchange diffchange-inline">modeling </ins>the <ins class="diffchange diffchange-inline">oxidation </ins>of [http://www.sigmaaldrich.com/catalog/product/sigma/a1888?lang=de&region=DE ABTS], a redox mediator of the laccase. ABTS <ins class="diffchange diffchange-inline">is used </ins>in this case only as placeholder, until suitable data for the substrates <ins class="diffchange diffchange-inline">are available</ins>. In the following picture a few possible reactions of our laccase <ins class="diffchange diffchange-inline">are shown</ins>. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_model.jpg|center|800px|thumb|<del class="diffchange diffchange-inline">Our Model </del>with different K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values. The span differs from 0.07 seconds to 1500 seconds.]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_model.jpg|center|800px|thumb|<ins class="diffchange diffchange-inline">The model </ins>with different K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values. The span differs from 0.07 seconds to 1500 seconds <ins class="diffchange diffchange-inline">to degrade all ABTS</ins>.]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We use K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values from 11 to 643500 that result in degradation time points from 0.07s to 1500s. Furthermore we try to integrate different temperatures to our model. In a span from 10°C to 30°C and a K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value of 1, the degradation differs from 40 s to 100s.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We use K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values from 11 to 643500 that result in degradation time points from 0.07s to 1500s. Furthermore we try to integrate different temperatures to our model. In a span from 10°C to 30°C and a K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value of 1, the degradation differs from 40 s to 100s.</div></td></tr>
</table>Isahuhttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Modell&diff=271729&oldid=prevIsahu: /* Extend the model with sewage plant data */2012-10-20T13:11:58Z<p><span class="autocomment">Extend the model with sewage plant data</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 13:11, 20 October 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The sewage plant in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and flow rate. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to degrade 80 % of the substrates. This will be the residence time. Combined with the actual flow rate we can determine the reactor size. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A 3D model of the required enzyme amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and flow rate.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The sewage plant in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and flow rate. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to degrade 80 % of the substrates. This will be the residence time. Combined with the actual flow rate we can determine the reactor size. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A 3D model of the required enzyme amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and flow rate.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_3d.jpg|600px|thumb|left|The required enzyme amount (z axis) for a sewage plant is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). <del class="diffchange diffchange-inline">Opimal </del>we will need 40 Kg laccase. In the worst case we will need 140 Kg ]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_3d.jpg|600px|thumb|left|The required enzyme amount (z axis) for a sewage plant is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). <ins class="diffchange diffchange-inline">Optimal </ins>we will need 40 Kg laccase. In the worst case we will need 140 Kg<ins class="diffchange diffchange-inline">. </ins>]]</div></td></tr>
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</table>Isahuhttp://2012.igem.org/wiki/index.php?title=Team:Bielefeld-Germany/Modell&diff=271727&oldid=prevIsahu: /* Extend the model with sewageplant data */2012-10-20T13:10:35Z<p><span class="autocomment">Extend the model with sewageplant data</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 13:10, 20 October 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We use K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values from 11 to 643500 that result in degradation time points from 0.07s to 1500s. Furthermore we try to integrate different temperatures to our model. In a span from 10°C to 30°C and a K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value of 1, the degradation differs from 40 s to 100s.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We use K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values from 11 to 643500 that result in degradation time points from 0.07s to 1500s. Furthermore we try to integrate different temperatures to our model. In a span from 10°C to 30°C and a K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value of 1, the degradation differs from 40 s to 100s.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==Extend the model with <del class="diffchange diffchange-inline">sewageplant </del>data==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==Extend the model with <ins class="diffchange diffchange-inline">sewage plant </ins>data==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_Schlossholte_Daten.jpg|600px|thumb|left|Date taken from the sewage plant located in Schloss Holte<del class="diffchange diffchange-inline">.</del>. Temperature minimum: 8.1 °C, maximum: 20,8 °C and average temperature 14.4 °C; pH minimum: 6.6, maximum: 7.2 and average 6.9; flow minimum: 39.2 L s<sup>-1</sup>, maximum 232.8 L s<sup>-1</sup> and <del class="diffchange diffchange-inline">avarage </del>76.8 L s<sup>-1</sup>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_Schlossholte_Daten.jpg|600px|thumb|left|Date taken from the sewage plant located in Schloss Holte. Temperature minimum: 8.1 °C, maximum: 20,8 °C and average temperature 14.4 °C; pH minimum: 6.6, maximum: 7.2 and average 6.9; flow minimum: 39.2 L s<sup>-1</sup>, maximum 232.8 L s<sup>-1</sup> and <ins class="diffchange diffchange-inline">average </ins>76.8 L s<sup>-1</sup>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The <del class="diffchange diffchange-inline">sewageplant </del>in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and <del class="diffchange diffchange-inline">flowrate</del>. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to <del class="diffchange diffchange-inline">degrdate </del>80 % of the substrates. This will be the residence time. Combined with the actual <del class="diffchange diffchange-inline">flowrate </del>we can determine the <del class="diffchange diffchange-inline">reactorsize</del>. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A 3D model of the required <del class="diffchange diffchange-inline">enzme </del>amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and <del class="diffchange diffchange-inline">flowrate</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The <ins class="diffchange diffchange-inline">sewage plant </ins>in Schloss Holte provided information about the discharge water, particularly water temperature, pH value and <ins class="diffchange diffchange-inline">flow rate</ins>. The temperature and pH value have a direct influence on the enzymatic activity. Dependent on the enzymatic activity we want to calculate the time our fixed-bed reactor will need to <ins class="diffchange diffchange-inline">degrade </ins>80 % of the substrates. This will be the residence time. Combined with the actual <ins class="diffchange diffchange-inline">flow rate </ins>we can determine the <ins class="diffchange diffchange-inline">reactor size</ins>. To estimate the feasibility we want to know how much enzyme has to be produced for a sewage plant. A 3D model of the required <ins class="diffchange diffchange-inline">enzyme </ins>amount dependent of K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> values and <ins class="diffchange diffchange-inline">flow rate</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_3d.jpg|600px|thumb|left|The required enzyme amount (z axis) for a <del class="diffchange diffchange-inline">sewageplant </del>is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). Opimal we will need 40 Kg laccase. In the worst case we will need 140 Kg ]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:Bielefeld2012_3d.jpg|600px|thumb|left|The required enzyme amount (z axis) for a <ins class="diffchange diffchange-inline">sewage plant </ins>is dependent on K<sub>cat</sub> K<sub>M</sub><sup>-1</sup> value (x axis) influenced by temperature and pH value and the flow rate (y axis). Opimal we will need 40 Kg laccase. In the worst case we will need 140 Kg ]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Isahu