Team:Bielefeld-Germany/Labjournal

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Revision as of 12:31, 27 June 2012 by Isahu (Talk | contribs)

Contents

Week 1 (30.04.-06.05.12)

  • Start of our WET LAB time.
  • Generating new competent E.coli cells.

Week 2 (07.05.- 13.05.12)

  • Doing the first steps to prepare a the Student Acadamy in cooperation with the CeBiTec. Finding suitable BioBricks to develop an coulourful and easy experiment. Trying to isolate the GFP and RFP containing plasmid and to transform an Plasmidmix to produce colored Agar-plates.
  • Cloning of CueO (E. coli), CopA (Xanthomonas campestris) and CotA (Bacillus pumilus) in pSB1C3-backbone.

Week 3 (14.05.- 20.05.12)

  • Successful PCRs of laccase genes from Thermus thermophilus HB27 and Bacillus halodurans C-125 with genomic DNA as template. The primers were designed with T7-Promotor-overhanging ends after prefix and HIS-Tag-overhang before suffix.

Week 4 (21.05.- 27.05.12)

  • Cloning of CotA (Bacillus halodurans), Tth-laccase (Thermus thermophilus) and ccdB in pSB1C3-backbone.

Week 5 (28.05.- 03.06.12)

Week 6 (04.06.- 10.06.12)

Week 7 (11.06.- 17.06.12)

Week 8 (18.06.- 24.06.12)