Team:Austin Texas/Week of June 25 to July 2

From 2012.igem.org

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(→Notebook for Week of June 25 to July 2)
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=== Design of converter plasmid ===
=== Design of converter plasmid ===
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etc.
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*Razan
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**6/26 - Tuesday: First assembly PCR for converter and control plasmid.
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iGEM person
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**6/29 - Friday: Overlap PCR on gel extracted first PCR reactions to get LasR+Terminator, GFP+Terminator, and Las Promoter+CRE.
=== Design of Reporter Plasmid ===
=== Design of Reporter Plasmid ===

Revision as of 20:28, 6 July 2012

Contents

Notebook for week of June 25 to July 2

June 25

Design of converter plasmid

  • Razan
    • 6/26 - Tuesday: First assembly PCR for converter and control plasmid.
    • 6/29 - Friday: Overlap PCR on gel extracted first PCR reactions to get LasR+Terminator, GFP+Terminator, and Las Promoter+CRE.

Design of Reporter Plasmid

Spinach Aptamer

CBB5 Caffeine Inducible Promoters

  • Peter
    • 6/29 - Friday: Performed PCR using Ben's primers on ORF 1 site. Saved over weekend in 4C Fridge
    • 7/2 - Monday: Performed PCR using Ben's primers on BS_I20270 vector.
    • 7/3 - Tuesday: PCR purification on both PCRs. Gibson assembly of purified PCR. Gel Electrophoresis indicated that Gibson assembly was unsuccessful, as insert ORF 1 PCR was unsuccessful while vector PCR was successful. Erik independently performed PCR on the ORF 1. Used this, along with Dpn1 digested vector PCR to create a new Gibson.

Inducible Odor

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