Team:Austin Texas/Week of July 9 to July 15
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== Design of Converter Plasmid == | == Design of Converter Plasmid == | ||
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+ | *Razan | ||
+ | **7/9- Gibson Assembly of Converter and Control plasmids | ||
+ | **7/10- Transformation of gibson failed and gel shows no correct bands | ||
+ | **7/11- Redid Gibson assembly of Converter and COntrol plasmids with longer incubation time | ||
== Design of Reporter Plasmid == | == Design of Reporter Plasmid == |
Revision as of 20:24, 11 July 2012
Contents |
Notebook for week of July 9 to July 15
Design of Converter Plasmid
- Razan
- 7/9- Gibson Assembly of Converter and Control plasmids
- 7/10- Transformation of gibson failed and gel shows no correct bands
- 7/11- Redid Gibson assembly of Converter and COntrol plasmids with longer incubation time
Design of Reporter Plasmid
Spinach Aptamer
CBB5 Caffeine Inducible Promoters
- Peter
- 7/9 - Set up confirmation PCR of cultures. 1 for each culture, as well as 2 controls from CBB5 genomic DNA.
- 7/10 - Gel electrophoresis of confirmation PCR showed 2 successful Gibson product colonies, as well as both successful controls from CBB5 genomic DNA.