Team:Amsterdam/data/experimental

From 2012.igem.org

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[[File:Amsterdam_exp_fig_10.png|300px|right|thumb|Figure 10]]
[[File:Amsterdam_exp_fig_10.png|300px|right|thumb|Figure 10]]
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[[File:Amsterdam_exp_fig_14.png|300px|right|thumb|Figure 11]]
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[[File:Amsterdam_exp_fig_14.png|450px|right|thumb|Figure 11]]
The “off” state was not achieved in the absence of arabinose. All the experiments have been conducted into the high copy number plasmid pSB1AT3. It is known that leaky expression is relative to the copy number of the plasmid.1 Hence, the inability of the Cellular Logbook to show an “off” state in the absence of arabinose could be attributed to the high copy number plasmid used in these experiments as was discussed with the LacH.
The “off” state was not achieved in the absence of arabinose. All the experiments have been conducted into the high copy number plasmid pSB1AT3. It is known that leaky expression is relative to the copy number of the plasmid.1 Hence, the inability of the Cellular Logbook to show an “off” state in the absence of arabinose could be attributed to the high copy number plasmid used in these experiments as was discussed with the LacH.

Revision as of 03:33, 27 September 2012