Team:Amsterdam/data/experimental
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<h4>Behavior of the writer-reader module in varying growth conditions</h4> | <h4>Behavior of the writer-reader module in varying growth conditions</h4> | ||
- | [[File: | + | [[File:Amsterdam_exp_fig_4.png|300px|right|thumb|Figure 3]] |
We aimed to characterize the activity of our writer during bacterial growth. We performed a growth curve experiment (figure 3) and collected samples at different time points to test the occurrence of methylation over different times of growth. | We aimed to characterize the activity of our writer during bacterial growth. We performed a growth curve experiment (figure 3) and collected samples at different time points to test the occurrence of methylation over different times of growth. | ||
- | + | [[File:Amsterdam_exp_fig_3.png|300px|right|thumb|Figure 4]] | |
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Figure 4 shows ScaI digestion of plasmids extracted from samples activated or not by 10mM IPTG and collected between 0 and 12h. Both conditions reveal an intermediate restriction profile over. However, the two samples taken after 24 hours show a different result, i.e. almost only uncut plasmid is observed as a result of an significant increase of methylation of our reader. | Figure 4 shows ScaI digestion of plasmids extracted from samples activated or not by 10mM IPTG and collected between 0 and 12h. Both conditions reveal an intermediate restriction profile over. However, the two samples taken after 24 hours show a different result, i.e. almost only uncut plasmid is observed as a result of an significant increase of methylation of our reader. |
Revision as of 03:24, 27 September 2012