Team:Amsterdam/data/background activity

From 2012.igem.org

(Difference between revisions)
(Cell growth rate)
(Leaky expression rate)
Line 123: Line 123:
We immediately see that the $[O_{\text{Free}}]$ depends on $[R_{2}]$ and $O_{\text{Total}}$.
We immediately see that the $[O_{\text{Free}}]$ depends on $[R_{2}]$ and $O_{\text{Total}}$.
-
The dissociation constant of the dimerized LacI-repressor for its operator sequence has been determined to be $10^{-12} M $ and the volume of ''E. coli'' is $0.7\ \mu m^{3}$. Assuming $O_{\text{Total}} = 200$ and $R_{2} = 200$, the time fraction during which $O$ is free i s $2.10815 \cdot 10^{-6}$.
+
The dissociation constant of the dimerized LacI-repressor for its operator sequence has been determined to be $10^{-12} M $ and the volume of ''E. coli'' is $0.7\ \mu m^{3}$. Assuming $O_{\text{Total}} = 200$ and $R_{2} = 200$, the time fraction during which $O$ is free is $2.10815 \cdot 10^{-6}$.
-
More simply, the leaky expression rate can also be retrieved from this [http://oregonstate.edu/instruction/bb492/lectures/Regulation.html website], which states a 1000-fold decrease in expression of the repressor-bound operon compared to the free operon.  
+
More simply, the leaky expression rate can also be retrieved from this [http://oregonstate.edu/instruction/bb492/lectures/Regulation.html website], which states a 1000-fold decrease in expression of the repressor-bound operon compared to the free operon.
==== Fusion protein catalysis rate ====
==== Fusion protein catalysis rate ====

Revision as of 02:50, 27 September 2012