Team:Amsterdam

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<h2>Cellular Logbook - A methylation-based reporter system</h2>
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| <font color=black size=5><center>Abstract</center></font>
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'''Multi-sensing genetic devices''' offer great future perspectives for biotechnology, environmental monitoring and medical diagnostics. In light of this we have created an innovative '''epigenetic DNA-methylation''' based '''detection system''' in <i>E. coli</i>, named '''Cellular Logbook''', that has the potential of simultaneously reporting on significantly more signals than current fluorescence-based systems (eg. GFP). The Cellular Logbook can be used to '''detect (Sensor module) and store (Writer module)''' the presence of '''any compound''' linked to a transcriptional regulator. This system allows for '''offline monitoring''' by functioning as a '''memory module (Writer module)'''. Assessment of the memory status is performed by digesting with restriction endonucleases followed by gel electrophoresis '''(Reader Module)'''. Furthermore, the Cellular Logbook is able to infer the '''time of signal-onset''' or '''signal-intensity''' using the natural dilution of the registered signal’s due to cell division. In shourt our novel epigenetic memory module system could potentially be utilized as a '''mulit-sensor/time indicating platform''' for many groundbreaking technologies and applications (eg. to measure environmental changes such as toxic compounds).
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'''Multi-sensing genetic devices''' offer great future perspectives for biotechnology, environmental monitoring and medical diagnostics. In light of this we have created an innovative [[Team:Amsterdam/glossary#Methyltransferase|'''DNA-Methylation''']] based '''reporter system''' in E. coli, named Cellular Logbook, that has the potential of simultaneously reporting on significantly more signals than current [[Team:Amsterdam/glossary#Fluorescent_Protein|fluorescence-based systems]] (eg. GFP). The Cellular Logbook can be used to '''detect and store''' the presence of '''any compound''' linked to a transcriptional regulator. This system allows for '''offline monitoring''' by functioning as a '''memory module'''. Assessment of the memory status is performed by [[Team:Amsterdam/glossary#Digestion|digesting]] with [[Team:Amsterdam/glossary#Restriction_Enzyme|restriction endonucleases]] followed by [[Team:Amsterdam/glossary#Gel_Electrophoresis|gel electrophoresis]]. Furthermore, the Cellular Logbook is able to infer the '''time of signal-onset''' or '''signal-intensity''' using the natural dilution of the registered signal’s due to cell division. In short our exciting new memory module could potentially be utilized as a platform for many groundbreaking technologies.
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[[File:Amsterdam_thumbs_up.gif|20px]] <font color="#8C7853">[https://igem.org/Team.cgi?id=874 <font color="#8C7853">Team registration</font>]<br\>
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[[File:Amsterdam_thumbs_up.gif|20px]] Complete judging form <br\>
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[[File:Amsterdam_thumbs_up.gif|20px]] [https://2012.igem.org/Team:Amsterdam <font color="#8C7853">Team Wiki</font>]<br\>
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            <img src="https://static.igem.org/mediawiki/2012/d/d7/Amsterdam_Slide-1.jpg" />
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[[File:Amsterdam_thumbs_down.jpg|20px]] Present a poster and a talk at the iGEM Jamboree <br\>
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            <img src="https://static.igem.org/mediawiki/2012/6/65/Amsterdam_Slide-2.jpg" />
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[[File:Amsterdam_thumbs_up.gif|20px]] New biobrick <br\></font>
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            <img src="https://static.igem.org/mediawiki/2012/a/a9/Amsterdam_Slide-3.jpg" />
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<font color="#C0C0C0">[[File:Amsterdam_thumbs_up.gif|20px]] Working biobrick <br\>
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[[File:Amsterdam_thumbs_up.gif|20px]] Biobrick characterization <br\> </font>
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            <img src="https://static.igem.org/mediawiki/2012/3/3d/Amsterdam_Slide-5.png" />
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<font color="#FFD700">[[File:Amsterdam_thumbs_down.jpg|20px]] Improve an existing biobrick <br\>
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[[File:Amsterdam_thumbs_down.jpg|20px]] Collaboration <br\>
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[[File:Amsterdam_thumbs_up.gif|20px]] Human outreach <br\></font>
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| style="width:50%;"| <font color=black size=5><center>Project</center></font>
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| style="width:50%;"| <font color=black size=5><center>Modeling</center></font>
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<h2>Sponsors</h2>
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[https://2012.igem.org/Team:Amsterdam/project/description Project description]<br\>
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<a href="http://www.aimms.vu.nl/en/index.asp"><img src="https://static.igem.org/mediawiki/2012/1/12/Amsterdam_Logo_AIMMS.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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[https://2012.igem.org/Team:Amsterdam/project/applications/ Applications]<br\>
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<a href="http://www.genscript.com/"><img src="https://static.igem.org/mediawiki/2012/6/6e/Amsterdam_Logo_Genscript.jpeg" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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[https://2012.igem.org/Team:Amsterdam/project/FAQ/ Frequently Asked Questions]<br\>
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<a href="http://nbv.kncv.nl/"><img src="https://static.igem.org/mediawiki/2012/8/8e/Amsterdam_Logo_nbv.gif" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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[https://2012.igem.org/Team:Amsterdam/project/diary/ Diary]<br\>
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<a href="http://www.sysbio.nl/"><img src="https://static.igem.org/mediawiki/2012/0/01/Amsterdam_Logo_NISB.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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[https://2012.igem.org/Team:Amsterdam/project/protocols/ Protocols]<br\>
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<a href="http://www.uva-alumni.nl/page.aspx?pid=460"><img src="https://static.igem.org/mediawiki/2012/3/3f/Amsterdam_Logo_universiteitfonds.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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[https://2012.igem.org/Team:Amsterdam/project/flashPOC1 Flash Animation for the Proof of Concept]<br\>
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<a href="http://eu.idtdna.com/site"><img src="https://static.igem.org/mediawiki/2012/c/c4/Xxidt.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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<a href="http://www.vu.nl/nl/index.asp"><img src="https://static.igem.org/mediawiki/2012/8/81/Amsterdam2012VuLogoHeader.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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<a href="http://www.uva.nl/"><img src="https://static.igem.org/mediawiki/2012/e/e4/Amsterdam2012UvaLogoHeader.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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There are no models online yet<br\>
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Plasmid Design Tool: Under production<br\>
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Wiki Editting Tool: Under production<br\>
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[https://2012.igem.org/Team:Amsterdam/safety/questions Safety: questions]<br\>
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[https://2012.igem.org/Team:Amsterdam/safety/components Safety: components]<br\>
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Latest revision as of 03:56, 27 September 2012

Cellular Logbook - A methylation-based reporter system

Multi-sensing genetic devices offer great future perspectives for biotechnology, environmental monitoring and medical diagnostics. In light of this we have created an innovative epigenetic DNA-methylation based detection system in E. coli, named Cellular Logbook, that has the potential of simultaneously reporting on significantly more signals than current fluorescence-based systems (eg. GFP). The Cellular Logbook can be used to detect (Sensor module) and store (Writer module) the presence of any compound linked to a transcriptional regulator. This system allows for offline monitoring by functioning as a memory module (Writer module). Assessment of the memory status is performed by digesting with restriction endonucleases followed by gel electrophoresis (Reader Module). Furthermore, the Cellular Logbook is able to infer the time of signal-onset or signal-intensity using the natural dilution of the registered signal’s due to cell division. In shourt our novel epigenetic memory module system could potentially be utilized as a mulit-sensor/time indicating platform for many groundbreaking technologies and applications (eg. to measure environmental changes such as toxic compounds).

Sponsors

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