Team:Amsterdam

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<h2>Cellular Logbook - A methylation-based reporter system</h2>
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| <font color=black size=5><center>Abstract</center></font>
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'''Multi-sensing genetic devices''' offer great future perspectives for biotechnology, environmental monitoring and medical diagnostics. In light of this we have created an innovative '''epigenetic DNA-methylation''' based '''detection system''' in <i>E. coli</i>, named '''Cellular Logbook''', that has the potential of simultaneously reporting on significantly more signals than current fluorescence-based systems (eg. GFP). The Cellular Logbook can be used to '''detect (Sensor module) and store (Writer module)''' the presence of '''any compound''' linked to a transcriptional regulator. This system allows for '''offline monitoring''' by functioning as a '''memory module (Writer module)'''. Assessment of the memory status is performed by digesting with restriction endonucleases followed by gel electrophoresis '''(Reader Module)'''. Furthermore, the Cellular Logbook is able to infer the '''time of signal-onset''' or '''signal-intensity''' using the natural dilution of the registered signal’s due to cell division. In shourt our novel epigenetic memory module system could potentially be utilized as a '''mulit-sensor/time indicating platform''' for many groundbreaking technologies and applications (eg. to measure environmental changes such as toxic compounds).
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| style="width: 100%;"| <font color=black size=5><center>Medal requirements</center></font>
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Meet '''The Cellular Logbook''', a methylation based memory module which uses the naturally occurring phenomenon
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of '''DNA methylation''' to robustly store signals it has sensed in its environment. The
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Amsterdam iGEM 2012 team, consisting of six students, will dedicate the summer to
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                <img src="https://static.igem.org/mediawiki/2012/c/c8/Igem_02F.jpg" />
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the realization of this innovative and ambitious plan. This novel storage mechanism,
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redesignating an evolutionarly designed tested and proven principle for novel purposes,
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            <img src="https://static.igem.org/mediawiki/2012/d/d7/Amsterdam_Slide-1.jpg" />
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could be linked to any of the many biological sensors that are available in the DNA
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            <img src="https://static.igem.org/mediawiki/2012/6/65/Amsterdam_Slide-2.jpg" />
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parts registry. The Cellular Logbook therefore holds great promise as a detect & store–system for
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experimental and industrial purposes.
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[[File:Amsterdam_thumbs_up.gif|20px]] <font color="#8C7853">[https://igem.org/Team.cgi?id=874 <font color="#8C7853">Team registration</font>]<br\>
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            <img src="https://static.igem.org/mediawiki/2012/4/49/Amsterdam_Slide-6.jpg" />
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[[File:Amsterdam_thumbs_up.gif|20px]] Complete judging form <br\>
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[[File:Amsterdam_thumbs_up.gif|20px]] [https://2012.igem.org/Team:Amsterdam <font color="#8C7853">Team Wiki</font>]<br\>
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[[File:Amsterdam_thumbs_down.jpg|20px]] Present a poster and a talk at the iGEM Jamboree <br\>
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[[File:Amsterdam_thumbs_up.gif|20px]] New biobrick <br\></font>
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<font color="#C0C0C0">[[File:Amsterdam_thumbs_up.gif|20px]] Working biobrick <br\>
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[[File:Amsterdam_thumbs_up.gif|20px]] Biobrick characterization <br\> </font>
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<font color="#FFD700">[[File:Amsterdam_thumbs_down.jpg|20px]] Improve an existing biobrick <br\>
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[[File:Amsterdam_thumbs_down.jpg|20px]] Collaboration <br\>
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<h2>Sponsors</h2>
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[[File:Amsterdam_thumbs_up.gif|20px]] Human outreach <br\></font>
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<a href="http://www.aimms.vu.nl/en/index.asp"><img src="https://static.igem.org/mediawiki/2012/1/12/Amsterdam_Logo_AIMMS.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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<a href="http://www.genscript.com/"><img src="https://static.igem.org/mediawiki/2012/6/6e/Amsterdam_Logo_Genscript.jpeg" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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| style="width:50%;"| <font color=black size=5><center>Project</center></font>
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<a href="http://nbv.kncv.nl/"><img src="https://static.igem.org/mediawiki/2012/8/8e/Amsterdam_Logo_nbv.gif" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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| style="width:50%;"| <font color=black size=5><center>Modeling</center></font>
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<a href="http://www.sysbio.nl/"><img src="https://static.igem.org/mediawiki/2012/0/01/Amsterdam_Logo_NISB.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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<a href="http://www.uva-alumni.nl/page.aspx?pid=460"><img src="https://static.igem.org/mediawiki/2012/3/3f/Amsterdam_Logo_universiteitfonds.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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<a href="http://eu.idtdna.com/site"><img src="https://static.igem.org/mediawiki/2012/c/c4/Xxidt.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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<a href="http://www.vu.nl/nl/index.asp"><img src="https://static.igem.org/mediawiki/2012/8/81/Amsterdam2012VuLogoHeader.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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[https://2012.igem.org/Team:Amsterdam/project/description Project description]<br\>
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<a href="http://www.uva.nl/"><img src="https://static.igem.org/mediawiki/2012/e/e4/Amsterdam2012UvaLogoHeader.png" height="35" align="left" style="padding:10px; padding-top:20px;"></a>
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[https://2012.igem.org/Team:Amsterdam/project/applications/ Applications]<br\>
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[https://2012.igem.org/Team:Amsterdam/project/FAQ/ Frequently Asked Questions]<br\>
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[https://2012.igem.org/Team:Amsterdam/project/diary/ Diary]<br\>
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[https://2012.igem.org/Team:Amsterdam/project/protocols/ Protocols]<br\>
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[https://2012.igem.org/Team:Amsterdam/project/flashPOC1 Flash Animation for the Proof of Concept]<br\>
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There are no models online yet<br\>
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| style="width:50%;"| <font color=black size=5><center>Tools</center></font>
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| style="width:50%;"| <font color=black size=5><center>Outreach</center></font>
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Plasmid Design Tool: Under production<br\>
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Wiki Editting Tool: Under production<br\>
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[https://2012.igem.org/Team:Amsterdam/safety/questions Safety: questions]<br\>
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[https://2012.igem.org/Team:Amsterdam/safety/components Safety: components]<br\>
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Latest revision as of 03:56, 27 September 2012

Cellular Logbook - A methylation-based reporter system

Multi-sensing genetic devices offer great future perspectives for biotechnology, environmental monitoring and medical diagnostics. In light of this we have created an innovative epigenetic DNA-methylation based detection system in E. coli, named Cellular Logbook, that has the potential of simultaneously reporting on significantly more signals than current fluorescence-based systems (eg. GFP). The Cellular Logbook can be used to detect (Sensor module) and store (Writer module) the presence of any compound linked to a transcriptional regulator. This system allows for offline monitoring by functioning as a memory module (Writer module). Assessment of the memory status is performed by digesting with restriction endonucleases followed by gel electrophoresis (Reader Module). Furthermore, the Cellular Logbook is able to infer the time of signal-onset or signal-intensity using the natural dilution of the registered signal’s due to cell division. In shourt our novel epigenetic memory module system could potentially be utilized as a mulit-sensor/time indicating platform for many groundbreaking technologies and applications (eg. to measure environmental changes such as toxic compounds).

Sponsors

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