Team/CINVESTAV-IPN-UNAM MX/Oxigen Response.htm

From 2012.igem.org

(Difference between revisions)
Line 1: Line 1:
-
<html xmlns="http://www.w3.org/1999/xhtml"><head>
+
<html xmlns="http://www.w3.org/1999/xhtml">
-
<title>Home</title>
+
<head>
 +
<meta http-equiv="content-type" content="text/html; charset=utf-8" />
 +
<title>Rho</title>
-
<link rel="stylesheet" href="https://dl.dropbox.com/u/199422/Cinvestav/style.css" />
+
<style type="text/css">
 +
* {
 +
margin: 0;
 +
padding: 0;
 +
}
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+
body {
-
<style type="text/css">td img {display: block;}</style>
+
background: #FFFFFF;
 +
text-align: justify;
 +
font-family: Georgia, "Times New Roman", Times, serif;
 +
font-size: 12px;
 +
color: #000000;
 +
}
 +
 
 +
h1, h2, h3 {
 +
color: #333333;
 +
}
 +
 
 +
h1, h2 {
 +
margin-bottom: 20px;
 +
font-weight: normal;
 +
}
 +
 
 +
h1 {
 +
font-size: 197%;
 +
}
 +
 
 +
h2 {
 +
font-size: 152%;
 +
}
 +
 
 +
h3 {
 +
font-size: 100%;
 +
}
 +
 
 +
p, ul, ol {
 +
margin-bottom: 20px;
 +
line-height: 1.8em;
 +
}
 +
 
 +
ul, ol {
 +
margin-left: 3em;
 +
}
 +
 
 +
blockquote {
 +
padding-left: 3em;
 +
background: url(https://static.igem.org/mediawiki/2012/1/11/Img08.gif) no-repeat;
 +
font-style: italic;
 +
}
 +
 
 +
a:link {
 +
color: #1953A7;
 +
}
 +
 
 +
a:active {
 +
color: #ED5C0C;
 +
}
 +
 
 +
a:visited {
 +
color: #666666;
 +
}
 +
 
 +
a:hover {
 +
text-decoration: none;
 +
color: #067600;
 +
}
 +
 
 +
img {
 +
border: none;
 +
}
 +
 
 +
img.left {
 +
float: left;
 +
margin: 0 20px 10px 0;
 +
}
 +
 
 +
/* Menu */
 +
 
 +
#menu {
 +
width: 770px;
 +
height: 65px;
 +
margin: 0 auto;
 +
border-right: 1px solid #EDEBD5;
 +
}
 +
 
 +
#menu ul {
 +
margin: 0;
 +
padding: 0;
 +
list-style: none;
 +
}
 +
 
 +
#menu li {
 +
display: inline;
 +
font-size: 12px;
 +
color:#4E4E4E;
 +
}
 +
 
 +
#menu a {
 +
display: block;
 +
float: left;
 +
width: 95px;
 +
height: 45px;
 +
padding: 10px 0 0 0;
 +
border-left: 1px solid #EDEBD5;
 +
text-transform: lowercase;
 +
text-decoration: none;
 +
text-align: center;
 +
font-size: 135%;
 +
color: #BABABA;
 +
}
 +
 
 +
#menu a:hover {
 +
background: #F7F7F4;
 +
}
 +
 
 +
#menu .active a {
 +
background: #5dbb10;
 +
color: #FFFFFF;
 +
}
 +
 
 +
/* Header */
 +
 
 +
#header {
 +
width: 770px;
 +
height: 170px;
 +
margin: 0 auto;
 +
}
 +
 
 +
#header h1 {
 +
float: left;
 +
width: 220px;
 +
height: 100px;
 +
margin: 0;
 +
padding: 70px 0 0 0;
 +
background: url(https://static.igem.org/mediawiki/2012/0/0a/Img02.jpg) no-repeat;
 +
text-align: center;
 +
font-size: 36px;
 +
}
 +
 
 +
#header h2 {
 +
float: right;
 +
width: 530px;
 +
height: 86px;
 +
margin: 0;
 +
background: url(https://static.igem.org/mediawiki/2012/4/4a/Img03.jpg) no-repeat;
 +
padding: 84px 20px 0 0;
 +
text-align: right;
 +
}
 +
 
 +
#header a {
 +
text-transform: lowercase;
 +
text-decoration: none;
 +
font-style: italic;
 +
font-weight: normal;
 +
color: #FFFFFF;
 +
}
 +
 
 +
/* Page */
 +
 
 +
#page {
 +
width: 770px;
 +
margin: 0 auto;
 +
padding: 20px 0;
 +
}
 +
#context{
 +
float: left;
 +
width: 570px;
 +
padding-top:5px;
 +
}       
 +
/* menudecontenido */
 +
 
 +
#sidebar {
 +
float: right;
 +
width: 160px;
 +
padding: 15px;
 +
background: #EDEBD5;
 +
}
 +
 
 +
#sidebar h2 {
 +
margin: 0;
 +
padding: 5px 15px;
 +
color: #FFFFFF;
 +
}
 +
 
 +
#sidebar h3 {
 +
font-size: 60%;
 +
color: #FFFFFF;
 +
}
 +
 
 +
#sidebar ul {
 +
margin: 0;
 +
padding: 15px;
 +
list-style: none;
 +
}
 +
 
 +
#sidebar li {
 +
              font-size: 12px;
 +
  text-align:left;
 +
}
 +
 
 +
#sidebar a {
 +
color: #FFFFFF;
 +
}
 +
 
 +
.orangebox {
 +
background: url(https://static.igem.org/mediawiki/2012/b/b0/Img05.gif) repeat-y;
 +
}
 +
 
 +
.orangebox h2 {
 +
background: url(https://static.igem.org/mediawiki/2012/c/c2/Img06.gif) repeat-y;
 +
}
 +
 
 +
/* Footer */
 +
 
 +
#piedepagina {
 +
clear: both;
 +
height: 150px;
 +
padding: 25px 0;
 +
background: #EDEBD5 url(https://static.igem.org/mediawiki/2012/a/a5/Img04.gif) repeat-x;
 +
}
 +
 
 +
#sponsors{
 +
 +
float: left;
 +
width: 85px;
 +
padding-top:5px;
 +
background: #EDEBD5;
 +
 +
      }
 +
#text2
 +
{
 +
color: #333333;
 +
font-size: 152%;
 +
margin-bottom: 20px;
 +
font-weight: normal;
 +
}
 +
#refe
 +
{
 +
color: #000000;
 +
font-size:9;
 +
margin-bottom: 20px;
 +
font-weight: normal;
 +
}
 +
 
 +
.firstHeading {
 +
margin-bottom: .1em;
 +
visibility:hidden;
 +
}
 +
 
 +
</style>
 +
 
 +
<meta name="keywords" content="" />
 +
<meta name="description" content="" />
-
<!--Fireworks CS6 Dreamweaver CS6 target.  Created Tue Sep 25 17:05:18 GMT-0500 (Central Daylight Time (Mexico)) 2012-->
 
</head>
</head>
-
<body bgcolor="#ffffff">
+
<body>
-
<table style="display: inline-table;" border="0" cellpadding="0" cellspacing="0" width="950" bgcolor="">
+
<div id="menu">
-
<!-- fwtable fwsrc="INDEX.fw.png" fwpage="Page 1" fwbase="principal.jpg" fwstyle="Dreamweaver" fwdocid = "927795023" fwnested="0" -->
+
<ul>
-
  <tr>
+
<li><a href="home.htm">Home</a></li>
-
  <td><img src="images/spacer.gif" width="240" height="1" alt="" /></td>
+
<li><a href="#">Rhodofactory</a></li>
-
  <td><img src="images/spacer.gif" width="710" height="1" alt="" /></td>
+
<li><a href="promoterselection.htm">Promoter selection </a></li>
-
  <td><img src="images/spacer.gif" width="1" height="1" alt="" /></td>
+
<li><a href="#">results </a></li>
-
  </tr>
+
<li><a href="#">Perspectives</a></li>
 +
<li class="active"><a href="#">Modeling</a></li>
 +
<li><a href="aboutus.htm">About us</a> </li>
 +
<li><a href="#">outreach</a></li>
 +
</ul>
 +
</div>
 +
<!-- end #menu -->
 +
<div id="header">
 +
<h1><a href="#"></a></h1>
 +
<h2><span></span></h2>
 +
</div>
 +
<!-- end #header -->
 +
<div id="page">
 +
<div id="context">
 +
 +
<h1><em>Oxygen Control System: PrrA/PrrB two component regulation system! </em></h1>
 +
            </br>
 +
<p>Under high oxygen tension, the system remains inactive, when oxygen concentration decreases PrrB (kinase sensor) activates itself
 +
through an autophosphorylation mediated by the electron flux from the electron transport chain through PrrC. Then PrrB transfers the
 +
phosphate group to PrrA response regulator that binds the promoter sequence and recruits the RNA polymerase for promoting GFP
 +
transcription. (See the next video for a visual explanation).</p>
 +
<div align="center"><iframe width="480" height="360" src="http://www.youtube.com/embed/pgp6DzpNyA0" frameborder="0" allowfullscreen></iframe></div><br>
 +
<p id="text2">Our biobricks </p>
 +
<p>The first biobrick consists in the complete oxygen dependent system, PrrA, PrrB and PrrC, each one with a Ribosome Binding Site, under a
 +
Medium strength promoter (J23104), this first biobrick also it has the PrrA dependent promoter and GFP as a reporter gene</p>
 +
    <img src="https://static.igem.org/mediawiki/2012/4/45/Rodo04.jpg" alt="rodo04" width="560" height="116"><br>
 +
<p><br>
 +
  The second biobrick is just the PrrA dependent promoter and GFP as a reporter gene.</p>
 +
<p align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Rodo05.jpg" alt="rodo05" width="450" height="249"></p>
 +
<p id="text2">We were inspired in:</p>
 +
<p>This system is inspired in PrrBCA two component system from Rhodobacter sphaeroides, which is a master regulator involved in expression of
 +
approximately 850 genes, >20% of the genome (Kaplan & Eraso 2005) This system coordinately controls genes involved in the complex switch
 +
between aerobic and anaerobic conditions and the optimum use of reducing power. It also regulates gene expression involved in
 +
photosynthesis, carbon dioxide fixation, nitrogen fixation, hydrogen uptake, aerotaxis, denitrification, electron transport, aerobic and anaerobic
 +
respiration, and heme biosynthesis, and others. Thus emphasizing its global role (Elsen et.al 2004, Kaplan & Eraso 2005, Zeilstra-Ryalls &
 +
Kaplan 2004).</p>
 +
<hr>
 +
<p id="refe">References<br>
 +
1.
 +
Kaplan S, Eraso J, Roh JH. (2005). Interacting regulatory networks in the facultative
 +
photosynthetic bacterium, Rhodobacter sphaeroides 2.4.1. Biochem. Soc. Trans. 33:51–55<br>
-
<tr>
+
2.
-
  <td><img src="https://static.igem.org/mediawiki/2012/6/68/LOGOPRIN.jpg" width="240" height="178" alt="Logo Principal" /></td>
+
Zeilstra-Ryalls JH, Kaplan S. (2004). Oxygen intervention in the regulation of gene xpression: the photosynthetic
-
  <td bgcolor="01A79D"><ul class="nav">
+
bacterial paradigm. Cell. Mol. Life Sci. 61:417–36<br>
-
<li>
+
 
-
<a href="/Team/CINVESTAV-IPN-UNAM_MX/home.htm">Home<span class="flecha">&#9660;</span></a>
+
3.
-
</li>
+
Eraso JM, Kaplan S (2009) Regulation of gene expression by PrrA in Rhodobacter sphaeroides 2.4.1: role of
-
<li>
+
polyamines and DNA topology. J Bacteriol 2009, 191(13):4341-4352.
-
<a href="#">Rhodofactory<span class="flecha">&#9660;</span></a>
+
</p>
-
<ul>
+
-
<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Overview.htm">Overview<span class="flecha">&#9660;</span></a></li>
+
</div>
-
           
+
<div id="sidebar">
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Light Response.htm">Light Response<span class="flecha">&#9660;</span></a></li>
+
  <div id="updates" class="orangebox">
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Oxigen Response.htm">Oxigen Response<span class="flecha">&#9660;</span></a></li>
+
<h2>Rhodofactory</h2>
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Chassis.htm">Chassis<span class="flecha">&#9660;</span></a></li>
+
<ul>
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Perspective.htm">Perspective<span class="flecha">&#9660;</span></a></li>
+
<li><a href="Overview.htm" target="_parent">Overview</a></li>
-
+
<li><a href="Light_Response.htm">Light and Oxygen</a></li>
-
</ul>
+
<li><a href="Oxigen_Response.htm">Oxygen Response</a></li>
-
</li>
+
<li><a href="Chassis.htm">Chassis</a></li>
-
<li>
+
</ul>
-
<a href="">Results<span class="flecha">&#9660;</span></a>
+
-
<ul>
+
-
<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Biobricks.htm">Biobricks<span class="flecha">&#9660;</span></a></li>
+
-
           
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Notebook.htm">Notebook<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Protocol.htm">Protocol<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/palustris response.htm">R. palustris response<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/sphaeroides response.htm">R. sphaeroides response<span class="flecha">&#9660;</span></a></li>
+
-
</ul>
+
-
</li>
+
-
<li>
+
</div>
-
<a href="#">IGEM Contribution<span class="flecha">&#9660;</span></a>
+
</div>    
-
<ul>
+
  <div style="clear: both;">&nbsp;</div>
-
<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Construction.htm">Construction<span class="flecha">&#9660;</span></a></li>
+
</div>
-
           
+
<!-- end #page -->
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Metods.htm">Methods<span class="flecha">&#9660;</span></a></li>
+
<div id="piedepagina">
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Results.htm">Results<span class="flecha">&#9660;</span></a></li>
+
<p align="center"> Rhodofactory 2012 </p>
-
+
<div id="sponsors">
-
</ul>
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/8/8a/Icytdf.png" alt="icytdf" width="70" height="99" /></div>
-
</li>
+
-
<li>
+
-
<a href="/Team/CINVESTAV-IPN-UNAM_MX/Modelling.htm">Modeling<span class="flecha">&#9660;</span></a>
+
-
+
-
</li>   
+
-
   
+
-
    <li>
+
-
<a href="#">Team<span class="flecha">&#9660;</span></a>
+
-
<ul>
+
-
<li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Members.htm">Members<span class="flecha">&#9660;</span></a></li>
+
-
           
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Institute.htm">Institute<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Labs.htm">Labs<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Sponsor.htm">Sponsor<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Gallery.htm">Gallery<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Acknowledgments.htm">Acknowledgments<span class="flecha">&#9660;</span></a></li>
+
-
+
-
</ul>
+
-
</li>
+
-
    <li>
+
-
<a href="#">Outreach<span class="flecha">&#9660;</span></a>
+
-
<ul>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Human Practice.htm">Human Practices<span class="flecha">&#9660;</span></a></li>
+
-
            <li><a href="/Team/CINVESTAV-IPN-UNAM_MX/Safety.htm">Safety<span class="flecha">&#9660;</span></a></li>
+
-
</ul>
+
-
</li>
+
-
   
+
-
   
+
-
</ul></td>
+
-
</td>
+
-
  <td><img src="images/spacer.gif" width="1" height="150" alt="" /></td>
+
-
  </tr>
+
-
  <tr>
+
-
  <td colspan="2" >
+
-
 
+
-
  <div id="main">
+
-
<div id="bodys_r1_c2">
+
</div>
</div>
-
<div id="bodys_r1_c3">
+
<div id="sponsors">
-
   
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/6/67/Osli.png" alt="osli" width="88" height="46" /></div>
-
      <img src="https://static.igem.org/mediawiki/2012/2/28/Oxigen_response.fw.png" width="500" height="92" /> </div>
+
</div>
-
<div class="clearFloat"></div>
+
-
<div id="bodys_r2_c2"></div>
+
<div id="sponsors">
-
<div id="bodys_r2_c3">
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fb/Bio.png" alt="bio" width="73" height="99" /></div>
-
    <div class="Text">
+
</div>
-
   
+
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/d/d6/Fermentas.png" alt="fermentAS" width="82" height="45" /></div>
-
      <h1><strong>Oxygen Control System: PrrA/PrrB  two component system </strong></h1>
+
<div id="sponsors">
-
      <p>This regulatory  system can sense oxygen concentration and senda response, under high oxygen tension, the system remains  inactive, when oxygen concentration decreases PrrB (His sensor kinase) turns active  trough an autophosphorylation with help of PrrC, which transmit the signal from  electron transport chain with help. Then PrrB transmit the phosphate group to  PrrA response regulator that directly binds promoter and recruits RNA  polymerase to start GFP transcription.</p>
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/a/a3/Cinestav.png" alt="cinestav" width="74" height="99" /></div>
-
      <p><strong>How does it work?</strong></p>
+
</div>
-
      <p>This genetic system  consists in two modules, the first one is formed by <strong><em>prra, prrb </em></strong><em>and<strong> prrc</strong></em>coupled to <strong>B0030</strong> RBS  units, <strong>J23104</strong> Strong constitutive  promoter and <strong>B0014</strong> Double terminator  to form the transcription unit. We will evaluate the functioning of J23104,  B0030 and B0014 in a new chassis. The second module consists in <strong>J54103</strong> GFP generator (B0030 RBS + E1010  GFP + B0014 Double terminator) coupled to PpsR repressible promoter.</p>
+
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/9/9b/Genscript.png" alt="genscript" width="83" height="45" /></div>
-
      <p>&nbsp;</p>
+
<div id="sponsors">
-
      <p><img src="https://static.igem.org/mediawiki/2012/9/93/Oxi.JPG" width="636" height="231" /></p>
+
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/1/16/Unam.png" alt="unam" width="78" height="91" /></div>
-
<h2>&nbsp;</h2>
+
</div>
-
<p center><p><strong>Inspired in…</strong></p>
+
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/c/c9/Gto.png" alt="gto" width="84" height="46" /></div>
-
      <p>This  system is inspired in PrrBCA two component system from Rhodobacter sphaeroides,  which is a master regulator that is involved in expression of approximately 850  genes, <strong><em>&gt;</em>20% of the genome</strong> (Kaplan &amp; Eraso 2005) This system coordinately controls genes involved in the complex  switch between aerobic and anaerobic lifestyles and the optimum use of reducing  power. It also regulates expression of genes involved in photosynthesis,  carbon dioxide fixation, nitrogen fixation, hydrogen uptake, aerotaxis,  denitrification, electron transport, aerobic and anaerobic respiration, and  heme biosynthesis, among others, thus emphasizing its global role (Elsen et.al  2004, Kaplan &amp; Eraso 2005, Zeilstra-Ryalls &amp; Kaplan 2004). </p>
+
    <div id="sponsors">
-
      <p>&nbsp;</p>
+
      <div align="center"><img src="https://static.igem.org/mediawiki/2012/f/fa/Qimica.png" alt="quimica" width="72" height="80" /></div>
-
      <ol>
+
-
        <li> Kaplan  S, Eraso J, Roh JH. (2005). <strong>Interacting regulatory  networks in the facultative</strong></li>
+
-
      </ol>
+
-
      <p><strong>photosynthetic  bacterium, <em>Rhodobacter sphaeroides </em>2.4.1.</strong> <em>Biochem. Soc. Trans. </em>33:51–55</p>
+
-
      <ol>
+
-
        <li> Zeilstra-Ryalls JH, Kaplan S. (2004). <strong>Oxygen intervention in the regulation of  gene xpression: the photosynthetic bacterial paradigm</strong>. <em>Cell. Mol. Life  Sci. </em>61:417–36</li>
+
-
        <li>Eraso JM, Kaplan S  (2009) <strong>Regulation of gene expression by  PrrA in Rhodobacter sphaeroides 2.4.1: role of polyamines and DNA topology</strong>. J Bacteriol 2009, 191(13):4341-4352.</li>
+
-
      </ol>
+
-
</p>
+
-
      </p>
+
-
    </div>
+
     </div>
     </div>
-
<div class="clearFloat"></div>
+
<div id="sponsors"><img src="https://static.igem.org/mediawiki/2012/7/73/Valaner.png" alt="valaner" width="84" height="48" /></div>
 +
<div id="sponsors">
 +
  <div align="center"><img src="https://static.igem.org/mediawiki/2012/7/79/Ipn.png" alt="ipn" width="75" height="97" /></div>
 +
</div>
 +
</div>
</div>
-
 
+
<!-- end #footer -->
-
    </td>
+
-
  <td><img src="images/spacer.gif" width="1" height="500" alt="" /></td>
+
-
  </tr>
+
-
</table>
+
</body>
</body>
</html>
</html>

Revision as of 00:57, 24 October 2012

Rho

Oxygen Control System: PrrA/PrrB two component regulation system!


Under high oxygen tension, the system remains inactive, when oxygen concentration decreases PrrB (kinase sensor) activates itself through an autophosphorylation mediated by the electron flux from the electron transport chain through PrrC. Then PrrB transfers the phosphate group to PrrA response regulator that binds the promoter sequence and recruits the RNA polymerase for promoting GFP transcription. (See the next video for a visual explanation).


Our biobricks

The first biobrick consists in the complete oxygen dependent system, PrrA, PrrB and PrrC, each one with a Ribosome Binding Site, under a Medium strength promoter (J23104), this first biobrick also it has the PrrA dependent promoter and GFP as a reporter gene

rodo04


The second biobrick is just the PrrA dependent promoter and GFP as a reporter gene.

rodo05

We were inspired in:

This system is inspired in PrrBCA two component system from Rhodobacter sphaeroides, which is a master regulator involved in expression of approximately 850 genes, >20% of the genome (Kaplan & Eraso 2005) This system coordinately controls genes involved in the complex switch between aerobic and anaerobic conditions and the optimum use of reducing power. It also regulates gene expression involved in photosynthesis, carbon dioxide fixation, nitrogen fixation, hydrogen uptake, aerotaxis, denitrification, electron transport, aerobic and anaerobic respiration, and heme biosynthesis, and others. Thus emphasizing its global role (Elsen et.al 2004, Kaplan & Eraso 2005, Zeilstra-Ryalls & Kaplan 2004).


References
1. Kaplan S, Eraso J, Roh JH. (2005). Interacting regulatory networks in the facultative photosynthetic bacterium, Rhodobacter sphaeroides 2.4.1. Biochem. Soc. Trans. 33:51–55
2. Zeilstra-Ryalls JH, Kaplan S. (2004). Oxygen intervention in the regulation of gene xpression: the photosynthetic bacterial paradigm. Cell. Mol. Life Sci. 61:417–36
3. Eraso JM, Kaplan S (2009) Regulation of gene expression by PrrA in Rhodobacter sphaeroides 2.4.1: role of polyamines and DNA topology. J Bacteriol 2009, 191(13):4341-4352.

 

Rhodofactory 2012

icytdf
osli
bio
fermentAS
cinestav
genscript
unam
gto
quimica
valaner
ipn