http://2012.igem.org/wiki/index.php?title=Special:Contributions/Cyrpaut&feed=atom&limit=50&target=Cyrpaut&year=&month=2012.igem.org - User contributions [en]2024-03-28T19:10:03ZFrom 2012.igem.orgMediaWiki 1.16.0http://2012.igem.org/Team:Evry/TeamTeam:Evry/Team2012-10-27T03:58:31Z<p>Cyrpaut: </p>
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<h2>The members of the team come from different schools and universities:</h2><br />
<ul><br />
<li><a href="http://www.issb.genopole.fr/Education">Evry university, Master mSSB </a>(Synthetic and systemic biology)</li><br />
<li><a href="http://www.uvsq.fr/welcome-to-uvsq-207282.kjsp?RH=ACCUEIL-FR&RF=ACCUEIL-EN">Versailles Saint Quentin university</a>(Biology)</li><br />
<li><a href="http://www.univ-paris1.fr/">Paris 1 Pantheon-Sorbonne University </a>(Philosophy)</li><br />
<li><a href="http://www.ens.fr/?lang=en">Ecole Normale Supérieure</a>(Engineering)</li><br />
<li><a href="http://www.ecp.fr/lang/en/homepage">Ecole Centrale Paris </a>(Engineering)</li><br />
<li><a href="http://www.supbiotech.fr/en/presentation-supbiotech.aspx">Sup'Biotech Paris </a>(Biotechnology)</li><br />
<li><a href="http://www.esiee.fr/en/">ESIEE Management </a>(Biotechnology)</li><br />
<li><a href="http://www.telecom-sudparis.eu/en_accueil.html>Télécom Sud Paris )</a>(Telecommunication Engineering & Managment</li><br />
<li><a href="http://www.epita.fr/masters/">Epita </a>(Computer Science)</li><br />
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<center><h1><a name="envi" style="text-decoration:none; color: white;">Our environment: a key location for synthetic biology in France</a></h1></center><br />
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<h2>Environment</h2><br />
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<a href="http://www.issb.genopole.fr/"><img src="https://static.igem.org/mediawiki/2012/b/bf/Photo_iSSB.jpeg" alt="logo issb" align="left" height="200px"/></a><br />
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<h3>The Institute of Systems and Synthetic Biology (iSSB):</h3><br />
<p><br />
iSSB is located on the Genopole® in Evry, host team in the summer. The iSSB is a laboratory at the University of Evry and CNRS, supported by Genopole®. It is a multidisciplinary environment where collaborate physicists, chemists, computer scientists and biologists. It is also the laboratory who founded and directs the Master 2 Systems Biology Synthetic and MSSB. This master of avant-garde, unique in France, offers courses provided by researchers at the forefront of their field, which guaranteed training in the state of the art techniques used in synthetic biology.<br/><br/><br />
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<h2>Collaborations</h2><br />
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<a href="http://www.lapaillasse.org/"><img src="https://static.igem.org/mediawiki/2012/c/c4/Logo_lapaillasse.png" alt="logo la paillasse" align="left" height="200px"/></a><br />
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<h3>La Paillasse: The Paris community Lab for Biotech</h3><br />
<p><br />
La Paillasse is a physical and web platform for citizen scientists, amateur biologists, researchers and entrepreneurs that fosters open-science, debates and hands-on practice of Biotechnology. La Paillasse is also the first and largest community laboratory for Biotech in France. This year, La Paillasse and some of its members are joining the team of Evry to participate in the design and the realization of one of the coolest iGEM project ever: The french froggies. During the summer, La Paillasse have hosted and organized meet-ups between citizens and the iGEM team for explaining the stakes of our iGEM projects and of Synthetic Biology in general. More in the Human practice section. <a href="http://www.lapaillasse.org">La Paillasse website! </a><br />
</p><br />
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<h1>Lab team</h1><br />
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<table id="team" cellspacing="10" ><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2012/1/19/Photo_Tristan.jpeg" alt="tristan" align="left" height="150px" style="padding:5px;"/><br />
<h3>Tristan Cerisy</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
I did my bachelor at Evry’s University, in Bioinformatics. During that year when Jean-loup Faulon presented iGem and synthetic biology, I found it very interesting. I wanted to participate with iGem from my bachelor. Because no team existed yet close to our university, William and I decided to create this team in November and we looked for interested people, funding and projects. I am very excited to be involved in this project with this wonderful team.<br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/68/Jorge2.png" alt="jorge" align="left" height="150px" style="padding:5px;"/><br />
<h3>Jorgelindo Da Veiga Moreira</h3><br />
<i>Master 1, AIV, Diderot University</i><br/><br/><br />
<p><br />
I’m a bachelor graduated student from a Parisian engineering school. I look forward to a Master degree in biotechnology. I discovered synthetic biology mainly through conferences and I’ve been immediately fascinated by this new biological approach to work on living systems. iGEM is a good opportunity for me to start in this field and to acquire experience for my future engineering carrier.<br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2012/d/d4/Photo_Carolina.jpeg" alt="carolina" align="left" height="150px" style="padding:5px;"/><br />
<h3>Carolina Gallo Lopez</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
Having acquired a background in biology and a first year of master in “Genomics, Cells, Development and Evolution” at the University of Paris Sud 11, I started to be interested in systems biology since my last year of bachelor and decided on getting involved in this approach during both my second year of master and my master’s thesis. I am participating in the iGEM competition as it allows me to combine experimental work with theoretical modelling. I am keen to learn not only different biological techniques and modeling approaches but also to learn from my teammates and other iGEM teams.<br />
</p><br />
</td><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2012/8/8e/Photo_Tiffany.jpeg" alt="tiffany" align="left" height="150px" style="padding:5px;"/><br />
<h3>Tiffany Souterre</h3><br />
<i>5th year, Sup'Biotech Paris</i><br/><br/><br />
<p><br />
After a BTS in Biotechnology, I went to Sup'Biotech, an engineering school in Biotechnology. For 6 years, I have been studying DNA manipulation, bacteria transformation... but it is only recently that I have heard about Synthetic Biology. It is such a promising and interesting field but requires multidisciplinary skills. I am eager to increase my knowledge to be able to work at the interface of biology and computer science. I discovered iGEM thanks to the 2009 Sup'Biotech team and I have no doubt it will be a great opportunity to learn more, gain experience and hopefully bring a modest contribution to Synthetic Biology.<br />
</p><br />
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<img src="https://static.igem.org/mediawiki/2012/a/a4/Photo_Will.jpeg" alt="william" align="left" height="150px" style="padding:5px;"/><br />
<h3>William Rostain</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
I did my bachelor in Edinburgh university, and came into contact with<br />
synthetic biology when I participated iGem with the 2010 Edinburgh team. My background is mostly molecular microbiology and biotechnology, but during iGEM I came into contact with modelling and how it could serve biology, thanks to the great modellers in our team. I decided to participate in mSSB in order to learn some more about modelling and programming so I could work more closely with computer people later :)<br />
</p><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/63/Photo_Cyrille.jpeg" alt="Cyrille" align="left" height="150px" style="padding:5px;"/><br />
<h3>Cyrille Pauthenier</h3><br />
<i>Student of the Ecole Normale Supérieure and member of mSSB</i><br/><br/><br />
<p><br />
I participated in the 2011 iGEM Paris-Bettencourt team who was a finalist and won the prize for best presentation at the European semi-final, and then sweet sixteen at the MIT. I've studied this year in the mSSB master, I'm now about to start a PhD in metabolic engineering at Jean-Loup Faulon's laboratory at iSSB.<br />
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<img src="https://static.igem.org/mediawiki/2012/5/57/Photo_Karine.jpeg" alt="karine" align="left" height="150px" style="padding:5px;"/><br />
<h3>Karine Chauris</h3><br />
<i>5th year, Sup'Biotech Paris</i><br/><br/><br />
<p><br />
I’m currently in my final year at Sup'Biotech Paris, a school of biotechnology, and my professional aim is to work on bioproduction processes. I’m attracted, since I was young, by all the possibilities of DNA manipulation and discovered synthetic biology with the first synthetic bacteria. Why did I join iGEM? It's a unique chance to participate in a challenging and concrete project.<br />
</p><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2012/e/eb/Jo2.png" alt="joachim" align="left" height="150px" style="padding:5px;"/><br />
<h3>Joachim Eeckhout</h3><br />
<i>4th year, Sup'Biotech Paris</i><br/><br/><br />
<p><br />
I'm in my fourth year at Sup'Biotech, an engeenering school in biotechnologies. Like many others, I have been amazed by the controversy surrounding the publication of Craig Venter and his synthetic bacterium "Synthia". Since then, I am very interested to acquire expertise in this field and the iGEM competition is a chance for me to be part of this new science.<br />
</p><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2012/b/b0/Photo_Raphael.jpeg" alt="raphael" align="left" height="150px" style="padding:5px;"/><br />
<h3>Raphael Ferreira</h3><br />
<i>1st year, AIV</i><br/><br/><br />
<p><br />
I've currently finished my license (Bachelor) in biotechnology and I'm about to join the AIV (Interdisciplinary approaches to life science) Masters. I discovered synthetic biology with the Craig Venter's paper (released in may 2010). After that, my school gave me a 5 month research & information processing project on synthetic biology. Through this project I've discovered the iGEM competition and the opportunities surrounding the emergence of this science filed. Enrolling in an iGEM team will give me a sight of how synthetic biology experimentations are, and also, to work with people who are interested in this science too.<br />
</p><br />
</td><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/61/Photo_PierreYves.jpeg" alt="pierre-yves" align="left" height="150px" style="padding:5px;"/><br />
<h3>Pierre Yves Nogue</h3><br />
<i>2nd year Biology degree, Versaille Saint-Quentin University</i><br/><br/><br />
<p><br />
Biologist student in Paris university, I've discovered the synthetic biology when I've joined "La Paillasse", a biohackspace in Paris. In the same time, I've learned about the existence of the Igem competition, and decided to join the Evry team after some meetings. Indeed, iGEM looks for me as a very good opportunity to work on really interesting subjects in an encouraging work atmosphere, and I've found all this advantages in the Evry team!<br />
</p><br />
</td><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2012/7/70/Photo_Hafez.jpeg" alt="hafez" align="left" height="150px" style="padding:5px;"/><br />
<h3>Hafez El-Sayyed</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
I did my bachelor's degree and my first Master year In Beirut Arab University, Lebanon, In Biochemistry and Molecular Biology. I came across the term synthetic Biology on the mSSB Website and then started doing some research about the Synthetic Biology topic. Now I want to be part of this wave of unorthodox and ingenious attempts to help create better things around us. I COULDN'T BE Prouder <br />
to be part of IGEM EVRY 2012.<br/><br />
</p><br />
</td><br />
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<h1>Modeling team</h1><br />
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<table id="team" cellspacing="10"><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2012/b/b6/Photo_Artemi.jpeg" alt="artémis" align="left" height="150px" style="padding:5px;"/><br />
<h3>Artémis Llamosi</h3><br />
<i>3rd year, Ecole Centrale Paris</i><br/><br/><br />
<p><br />
After completing a Master in engineering at École Centrale Paris and a research master in applied mathematics for biology at Paris 6 University, I am to start a PhD thesis on real-time control of biological systems on microfluidics chips. For many years interested in the relation between maths and biology, I discovered synthetic biology in 2011 through its connection to systems biology and immediately got "infected". My expertise being mostly on theoretical aspects, I enrolled in iGEM to get closer to the wetlab and apply my scholar knowledge to real life problems.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/b/b9/Photo_Pierre.jpeg" alt="pierre" align="left" height="150px" style="padding:5px;"/><br />
<h3>Pierre Parutto</h3><br />
<i>5th year, Epita</i><br/><br/><br />
<p><br />
I am curently in 5th year at the engineering school Epita and<br />
specialized in scientific computations. I am interested in biology<br />
since high school and more especially in the links between biological<br />
systems and my speciality: computer science. After all a cell can be<br />
seen as a kind of computer, as seen in the name "genetic code". I learned about the IGEM comptetition when I discovered the synthetic biology field in an article in Nature a few years ago. Since then I<br />
wanted to participate to the comptetition but never had the<br />
opportunity. I bring to the team my programming and engineering skills and hope to<br />
learn a lot from biologist in the lab.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/2/22/Photo_Iryna.jpeg" alt="iryna" align="left" height="150px" style="padding:5px;"/><br />
<h3>Iryna Nikolayeva</h3><br />
<i>2nd year, Telecom Sud Paris</i><br/><br/><br />
<p><br />
As specialization of my last year in the engineering school Telecom SudParis, I will be doing the mSSB (master in systemic and synthetic biology). I've got useful computer science and maths skills for modeling and making the wiki. I imagine that mixing technologies and life science can lead to exciting inventions and discoveries. The iGEM seemed to me a nice opportunity to get to know the synthetic biology background and interact with students that are interested in it!<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/66/M%26m.jpg" alt="mohamed" align="left" height="150px" style="padding:5px;"/><br />
<h3>Mohamed Machat</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
I am a civil engineer from Tunisia. My penchant for genetics has started since my first biology lectures in college. However, the first opportunity that I got to go into this field showed up in 2011, when I was admissible to join the ISSB master program. So I left my engineer job and have moved to France. The adventure of iGem looks to me a good step towards my career ambition: invest my mathematics and mechanics background into oncology!!<br />
</p><br />
</td><br />
</tr><br />
</table><br />
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<h1>Human practice</h1><br />
<br />
<table><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/a/a5/Photo_Clement.jpeg" alt="clément" align="left" height="150px" style="padding:5px;"/><br />
<h3>Clément Marquet</h3><br />
<i>Master 2, Philosophy, Paris I Pantheon-Sorbonne University</i><br/><br/><br />
<p><br />
I'm completing a master of philosophy of science at Paris 1 Pantheon Sorbonne University. I discovered synthetic biology about two years ago in a popular scientific review and was struck by the ambition of the field and the diversity of disciplines involved in it. I was seduced by the special place that laboratories such as Synberc give to social sciences and started thinking about some epistemological questions that could be raised by Feynman’s word on knowing and making or by the convergence of biology and technology. I saw in the iGEM contest an opportunity to discover scientific work from the inside and to experiment how philosophical reflections could get practical and debated inside a scientific group.<br />
</p><br />
</td><br />
</tr><br />
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<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/1/1e/Chassis.jpg" alt="chassis" align="left" width="180px" style="padding:5px;"/><br />
<h3>Chassis</h3><br />
<i>Function: Mascott</i><br/><br/><br />
<p><br />
Hey I just met you! I am chassis, Team Evry's famous mascott! I kind of enjoy synthetic biology since I heard I could serve science and represent <i> Xenopus tropicalis </i> tough condition! In my lost moments I really enjoy French wine, maybe we'll share a bottle or two! See you in Boston! <a href="https://www.facebook.com/chassis.igemevry"> Feel free to contact me on facebook! </a><br />
<br />
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</td><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/63/Frogandwine.jpg" alt="chassisandwine" align="left" width="150px"/><br />
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<h1>Instructors</h1><br />
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<table id="team" cellspacing="10"><br />
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<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/68/Photo_Alfonso.jpeg" alt="alfonso" align="left" height="150px"/><br />
<h3>Dr. Alfonso Jaramillo</h3><br />
<i>Group leader of the Synth-Bio team at iSSB</i><br/><br />
<a href="mailto:alfonso.jaramillo@gmail.com">Contact</a><br/><br/><br />
<p><br />
After a PhD in theoretical physics, he was converted to synthetic biology for over 10 years. He gained international recognition in this field. He is now the team director of the Bio-Synth iSSB, working on the design, synthesis and characterization of biological regulatory artificial pathways. He is also one of the main teachers of the mSSB (synthetic and systematic biology master). Alfonso Jaramillo will be our main supervisor. He led Valencia iGEM team in 2006, and participated in the supervision of Valencia's and Paris' teams.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/5/55/Photo_Thomas.jpeg" alt="thomas" align="left" height="150px"/><br />
<h3>Thomas Landrain</h3><br />
<i>PhD candidate in Jaramillo's Group at iSSB. Co-founder and President of La Paillasse</i><br/><br />
<a href="mailto:thomas.landrain@gmail.com">Contact</a><br/><br/><br />
<p><br />
Ex-student of the Ecole Normale Superieure de Paris, he is now a PhD student at iSSB in Alfonso Jaramillo's Group where he is developing new technologies, using the properties of RNA molecules, for analyzing and controlling cell fate in bacteria. He is also the co-founder and president of the first community lab for biotechnology in France "La Paillasse", affiliated with the DIYbio world movement. In 2007, he was one of the founder and participants of the first french iGEM team that became finalist of the competition and received the first prize for foundational research.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/d/d4/Photo_Andrew.jpeg" alt="andrew" align="left" height="150px"/><br />
<h3>Dr. Andrew Tolonen</h3><br />
<i>Group leader at Genoscope</i><br/><br />
<a href="mailto:atolonen@gmail.com">Contact</a><br/><br/><br />
<p><br />
After a PhD in genetics and genomics of cyanobacteria at MIT and a post-doc in the team of George Church at Harvard, one of the largest synthetic biology laboratories in the world, he is now a researcher at Genoscope in Evry. His work focuses on the manufacture of biofuels by cyanobacteria. Andrew Tolonen attends the our team in the selection and construction of the project. He participated in the supervision of Harvard iGEM teams during his post-PhD.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/d/d8/Photo_Nicolas.jpg" alt="nicolas" align="left" height="150px"/><br />
<h3>Dr. Nicolas Pollet</h3><br />
<i>Group leader of the Metamorphosis team at iSSB</i><br/><br />
<a href="mailto:nicolas.pollet@issb.genopole.fr">Contact</a><br/><br/><br />
<p><br />
After a PhD in developmental physiology at INSERM, he was a Marie Curie post-doctoral fellow at the German Cancer Research Institute in Heidelberg. He is now a CNRS senior scientist, head of the Metamorphosys group at iSSB working in genomics and systems biology using the Xenopus frog model. Nicolas Pollet will participate to IGEM as supervisor for the first time this year.<br />
</p><br />
</td><br />
</tr><br />
</table><br />
<br />
<h1>Advisors</h1><br />
<br />
<table id="team" cellspacing="10"><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/6b/Photo_Anna.jpeg" alt="ania" align="left" height="150px"/><br />
<h3>Anna Młynarczyk</h3><br />
<i>Doctoral student in proteomics at the university of Evry</i><br/><br/><br />
<p><br />
She obtained a Master in Biotechnology at the Westpomeranian University of Technology in Szczecin in Poland. During her university studies she has conducted several training courses abroad (UEVE, TEPAK Cyprus) and in Poland which made her want to do research. Anna also performs the tutoring, which allows her to develop teaching skills.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/f/fa/Aurore.png" alt="aurore" align="left" height="150px"/><br />
<h3>Dr. Aurore THELIE</h3><br />
<i>PostDoc at Dr. Pollet's lab</i><br/><br/><br />
<p><br />
After a molecular biology training and a PhD in Reproductive Biology at INRA, she was a post-doctoral fellow at the Institute of Molecular Biology and Medicine (IBMM) in Belgium where she discovered the Xenopus model and all opportunity of study it offers. In 2012 she joined the Metamorphosys group at iSSB to study motoneurons and construct Xenopus transgenic lines using synthetic biology tools.<br />
</p><br />
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</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/TeamTeam:Evry/Team2012-10-27T03:56:05Z<p>Cyrpaut: </p>
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<center><br />
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<img src="https://static.igem.org/mediawiki/2012/f/f8/Schools.gif" alt="Gif_Evry" width="380" height ="380" style="float: right;"/><br />
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<br/><br />
<h2>The members of the team come from different schools and universities:</h2><br />
<ul><br />
<li><a href="http://www.issb.genopole.fr/Education">Evry university, Master mSSB </a>(Synthetic and systemic biology)</li><br />
<li><a href="http://www.uvsq.fr/welcome-to-uvsq-207282.kjsp?RH=ACCUEIL-FR&RF=ACCUEIL-EN">Versailles Saint Quentin university</a>(Biology)</li><br />
<li><a href="http://www.univ-paris1.fr/">Paris 1 Pantheon-Sorbonne University </a>(Philosophy)</li><br />
<li><a href="http://www.ens.fr/?lang=en">Ecole Normale Supérieure</a>(Engineering)</li><br />
<li><a href="http://www.ecp.fr/lang/en/homepage">Ecole Centrale Paris </a>(Engineering)</li><br />
<li><a href="http://www.supbiotech.fr/en/presentation-supbiotech.aspx">Sup'Biotech Paris </a>(Biotechnology)</li><br />
<li><a href="http://www.esiee.fr/en/">ESIEE Management </a>(Biotechnology)</li><br />
<li><a href="http://www.telecom-sudparis.eu/en_accueil.html>Télécom Sud Paris )</a>(Telecommunication Engineering & Managment</li><br />
<li><a href="http://www.epita.fr/masters/">Epita </a>(Computer Science)</li><br />
</ul><br />
<br />
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<center><h1><a name="envi" style="text-decoration:none; color: white;">Our environment: a key location for synthetic biology in France</a></h1></center><br />
<br />
<h2>Environment</h2><br />
<br />
<br />
<a href="http://www.issb.genopole.fr/"><img src="https://static.igem.org/mediawiki/2012/b/bf/Photo_iSSB.jpeg" alt="logo issb" align="left" height="200px"/></a><br />
<br />
<h3>The Institute of Systems and Synthetic Biology (iSSB):</h3><br />
<p><br />
iSSB is located on the Genopole® in Evry, host team in the summer. The iSSB is a laboratory at the University of Evry and CNRS, supported by Genopole®. It is a multidisciplinary environment where collaborate physicists, chemists, computer scientists and biologists. It is also the laboratory who founded and directs the Master 2 Systems Biology Synthetic and MSSB. This master of avant-garde, unique in France, offers courses provided by researchers at the forefront of their field, which guaranteed training in the state of the art techniques used in synthetic biology.<br/><br/><br />
</p><br />
<br><br><br />
<br />
<h2>Collaborations</h2><br />
<br />
<a href="http://www.lapaillasse.org/"><img src="https://static.igem.org/mediawiki/2012/c/c4/Logo_lapaillasse.png" alt="logo la paillasse" align="left" height="200px"/></a><br />
<br />
<h3>La Paillasse: The Paris community Lab for Biotech</h3><br />
<p><br />
La Paillasse is a physical and web platform for citizen scientists, amateur biologists, researchers and entrepreneurs that fosters open-science, debates and hands-on practice of Biotechnology. La Paillasse is also the first and largest community laboratory for Biotech in France. This year, La Paillasse and some of its members are joining the team of Evry to participate in the design and the realization of one of the coolest iGEM project ever: The french froggies. During the summer, La Paillasse have hosted and organized meet-ups between citizens and the iGEM team for explaining the stakes of our iGEM projects and of Synthetic Biology in general. More in the Human practice section. <a href="http://www.lapaillasse.org">La Paillasse website! </a><br />
</p><br />
<br><br><br><br><br />
<br />
<h1>Lab team</h1><br />
<br />
<table id="team" cellspacing="10" ><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/1/19/Photo_Tristan.jpeg" alt="tristan" align="left" height="150px"/><br />
<h3>Tristan Cerisy</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
I did my bachelor at Evry’s University, in Bioinformatics. During that year when Jean-loup Faulon presented iGem and synthetic biology, I found it very interesting. I wanted to participate with iGem from my bachelor. Because no team existed yet close to our university, William and I decided to create this team in November and we looked for interested people, funding and projects. I am very excited to be involved in this project with this wonderful team.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/68/Jorge2.png" alt="jorge" align="left" height="150px"/><br />
<h3>Jorgelindo Da Veiga Moreira</h3><br />
<i>Master 1, AIV, Diderot University</i><br/><br/><br />
<p><br />
I’m a bachelor graduated student from a Parisian engineering school. I look forward to a Master degree in biotechnology. I discovered synthetic biology mainly through conferences and I’ve been immediately fascinated by this new biological approach to work on living systems. iGEM is a good opportunity for me to start in this field and to acquire experience for my future engineering carrier.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/d/d4/Photo_Carolina.jpeg" alt="carolina" align="left" height="150px"/><br />
<h3>Carolina Gallo Lopez</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
Having acquired a background in biology and a first year of master in “Genomics, Cells, Development and Evolution” at the University of Paris Sud 11, I started to be interested in systems biology since my last year of bachelor and decided on getting involved in this approach during both my second year of master and my master’s thesis. I am participating in the iGEM competition as it allows me to combine experimental work with theoretical modelling. I am keen to learn not only different biological techniques and modeling approaches but also to learn from my teammates and other iGEM teams.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/8/8e/Photo_Tiffany.jpeg" alt="tiffany" align="left" height="150px"/><br />
<h3>Tiffany Souterre</h3><br />
<i>5th year, Sup'Biotech Paris</i><br/><br/><br />
<p><br />
After a BTS in Biotechnology, I went to Sup'Biotech, an engineering school in Biotechnology. For 6 years, I have been studying DNA manipulation, bacteria transformation... but it is only recently that I have heard about Synthetic Biology. It is such a promising and interesting field but requires multidisciplinary skills. I am eager to increase my knowledge to be able to work at the interface of biology and computer science. I discovered iGEM thanks to the 2009 Sup'Biotech team and I have no doubt it will be a great opportunity to learn more, gain experience and hopefully bring a modest contribution to Synthetic Biology.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/a/a4/Photo_Will.jpeg" alt="william" align="left" height="150px"/><br />
<h3>William Rostain</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
I did my bachelor in Edinburgh university, and came into contact with<br />
synthetic biology when I participated iGem with the 2010 Edinburgh team. My background is mostly molecular microbiology and biotechnology, but during iGEM I came into contact with modelling and how it could serve biology, thanks to the great modellers in our team. I decided to participate in mSSB in order to learn some more about modelling and programming so I could work more closely with computer people later :)<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/63/Photo_Cyrille.jpeg" alt="Cyrille" align="left" height="150px"/><br />
<h3>Cyrille Pauthenier</h3><br />
<i>Student of the Ecole Normale Supérieure and member of mSSB</i><br/><br/><br />
<p><br />
I participated in the 2011 iGEM Paris-Bettencourt team who was a finalist and won the prize for best presentation at the European semi-final, and then sweet sixteen at the MIT. I've studied this year in the mSSB master, I'm now about to start a PhD in metabolic engineering at Jean-Loup Faulon's laboratory at iSSB.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/5/57/Photo_Karine.jpeg" alt="karine" align="left" height="150px"/><br />
<h3>Karine Chauris</h3><br />
<i>5th year, Sup'Biotech Paris</i><br/><br/><br />
<p><br />
I’m currently in my final year at Sup'Biotech Paris, a school of biotechnology, and my professional aim is to work on bioproduction processes. I’m attracted, since I was young, by all the possibilities of DNA manipulation and discovered synthetic biology with the first synthetic bacteria. Why did I join iGEM? It's a unique chance to participate in a challenging and concrete project.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/e/eb/Jo2.png" alt="joachim" align="left" height="150px"/><br />
<h3>Joachim Eeckhout</h3><br />
<i>4th year, Sup'Biotech Paris</i><br/><br/><br />
<p><br />
I'm in my fourth year at Sup'Biotech, an engeenering school in biotechnologies. Like many others, I have been amazed by the controversy surrounding the publication of Craig Venter and his synthetic bacterium "Synthia". Since then, I am very interested to acquire expertise in this field and the iGEM competition is a chance for me to be part of this new science.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/b/b0/Photo_Raphael.jpeg" alt="raphael" align="left" height="150px"/><br />
<h3>Raphael Ferreira</h3><br />
<i>1st year, AIV</i><br/><br/><br />
<p><br />
I've currently finished my license (Bachelor) in biotechnology and I'm about to join the AIV (Interdisciplinary approaches to life science) Masters. I discovered synthetic biology with the Craig Venter's paper (released in may 2010). After that, my school gave me a 5 month research & information processing project on synthetic biology. Through this project I've discovered the iGEM competition and the opportunities surrounding the emergence of this science filed. Enrolling in an iGEM team will give me a sight of how synthetic biology experimentations are, and also, to work with people who are interested in this science too.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/61/Photo_PierreYves.jpeg" alt="pierre-yves" align="left" height="150px"/><br />
<h3>Pierre Yves Nogue</h3><br />
<i>2nd year Biology degree, Versaille Saint-Quentin University</i><br/><br/><br />
<p><br />
Biologist student in Paris university, I've discovered the synthetic biology when I've joined "La Paillasse", a biohackspace in Paris. In the same time, I've learned about the existence of the Igem competition, and decided to join the Evry team after some meetings. Indeed, iGEM looks for me as a very good opportunity to work on really interesting subjects in an encouraging work atmosphere, and I've found all this advantages in the Evry team!<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/7/70/Photo_Hafez.jpeg" alt="hafez" align="left" height="150px"/><br />
<h3>Hafez El-Sayyed</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
I did my bachelor's degree and my first Master year In Beirut Arab University, Lebanon, In Biochemistry and Molecular Biology. I came across the term synthetic Biology on the mSSB Website and then started doing some research about the Synthetic Biology topic. Now I want to be part of this wave of unorthodox and ingenious attempts to help create better things around us. I COULDN'T BE Prouder <br />
to be part of IGEM EVRY 2012.<br/><br />
</p><br />
</td><br />
</tr><br />
<br />
</table><br />
<br />
<h1>Modeling team</h1><br />
<br />
<table id="team" cellspacing="10"><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/b/b6/Photo_Artemi.jpeg" alt="artémis" align="left" height="150px"/><br />
<h3>Artémis Llamosi</h3><br />
<i>3rd year, Ecole Centrale Paris</i><br/><br/><br />
<p><br />
After completing a Master in engineering at École Centrale Paris and a research master in applied mathematics for biology at Paris 6 University, I am to start a PhD thesis on real-time control of biological systems on microfluidics chips. For many years interested in the relation between maths and biology, I discovered synthetic biology in 2011 through its connection to systems biology and immediately got "infected". My expertise being mostly on theoretical aspects, I enrolled in iGEM to get closer to the wetlab and apply my scholar knowledge to real life problems.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/b/b9/Photo_Pierre.jpeg" alt="pierre" align="left" height="150px"/><br />
<h3>Pierre Parutto</h3><br />
<i>5th year, Epita</i><br/><br/><br />
<p><br />
I am curently in 5th year at the engineering school Epita and<br />
specialized in scientific computations. I am interested in biology<br />
since high school and more especially in the links between biological<br />
systems and my speciality: computer science. After all a cell can be<br />
seen as a kind of computer, as seen in the name "genetic code". I learned about the IGEM comptetition when I discovered the synthetic biology field in an article in Nature a few years ago. Since then I<br />
wanted to participate to the comptetition but never had the<br />
opportunity. I bring to the team my programming and engineering skills and hope to<br />
learn a lot from biologist in the lab.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/2/22/Photo_Iryna.jpeg" alt="iryna" align="left" height="150px"/><br />
<h3>Iryna Nikolayeva</h3><br />
<i>2nd year, Telecom Sud Paris</i><br/><br/><br />
<p><br />
As specialization of my last year in the engineering school Telecom SudParis, I will be doing the mSSB (master in systemic and synthetic biology). I've got useful computer science and maths skills for modeling and making the wiki. I imagine that mixing technologies and life science can lead to exciting inventions and discoveries. The iGEM seemed to me a nice opportunity to get to know the synthetic biology background and interact with students that are interested in it!<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/66/M%26m.jpg" alt="mohamed" align="left" height="150px"/><br />
<h3>Mohamed Machat</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
I am a civil engineer from Tunisia. My penchant for genetics has started since my first biology lectures in college. However, the first opportunity that I got to go into this field showed up in 2011, when I was admissible to join the ISSB master program. So I left my engineer job and have moved to France. The adventure of iGem looks to me a good step towards my career ambition: invest my mathematics and mechanics background into oncology!!<br />
</p><br />
</td><br />
</tr><br />
</table><br />
<br />
<h1>Human practice</h1><br />
<br />
<table><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/a/a5/Photo_Clement.jpeg" alt="clément" align="left" height="150px"/><br />
<h3>Clément Marquet</h3><br />
<i>Master 2, Philosophy, Paris I Pantheon-Sorbonne University</i><br/><br/><br />
<p><br />
I'm completing a master of philosophy of science at Paris 1 Pantheon Sorbonne University. I discovered synthetic biology about two years ago in a popular scientific review and was struck by the ambition of the field and the diversity of disciplines involved in it. I was seduced by the special place that laboratories such as Synberc give to social sciences and started thinking about some epistemological questions that could be raised by Feynman’s word on knowing and making or by the convergence of biology and technology. I saw in the iGEM contest an opportunity to discover scientific work from the inside and to experiment how philosophical reflections could get practical and debated inside a scientific group.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/1/1e/Chassis.jpg" alt="chassis" align="left" width="180px" style="padding:5px;"/><br />
<h3>Chassis</h3><br />
<i>Function: Mascott</i><br/><br/><br />
<p><br />
Hey I just met you! I am chassis, Team Evry's famous mascott! I kind of enjoy synthetic biology since I heard I could serve science and represent <i> Xenopus tropicalis </i> tough condition! In my lost moments I really enjoy French wine, maybe we'll share a bottle or two! See you in Boston! <a href="https://www.facebook.com/chassis.igemevry"> Feel free to contact me on facebook! </a><br />
<br />
</p><br />
</td><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/63/Frogandwine.jpg" alt="chassisandwine" align="left" width="150px"/><br />
</td><br />
</tr><br />
</table><br />
<br />
<h1>Instructors</h1><br />
<br />
<table id="team" cellspacing="10"><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/68/Photo_Alfonso.jpeg" alt="alfonso" align="left" height="150px"/><br />
<h3>Dr. Alfonso Jaramillo</h3><br />
<i>Group leader of the Synth-Bio team at iSSB</i><br/><br />
<a href="mailto:alfonso.jaramillo@gmail.com">Contact</a><br/><br/><br />
<p><br />
After a PhD in theoretical physics, he was converted to synthetic biology for over 10 years. He gained international recognition in this field. He is now the team director of the Bio-Synth iSSB, working on the design, synthesis and characterization of biological regulatory artificial pathways. He is also one of the main teachers of the mSSB (synthetic and systematic biology master). Alfonso Jaramillo will be our main supervisor. He led Valencia iGEM team in 2006, and participated in the supervision of Valencia's and Paris' teams.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/5/55/Photo_Thomas.jpeg" alt="thomas" align="left" height="150px"/><br />
<h3>Thomas Landrain</h3><br />
<i>PhD candidate in Jaramillo's Group at iSSB. Co-founder and President of La Paillasse</i><br/><br />
<a href="mailto:thomas.landrain@gmail.com">Contact</a><br/><br/><br />
<p><br />
Ex-student of the Ecole Normale Superieure de Paris, he is now a PhD student at iSSB in Alfonso Jaramillo's Group where he is developing new technologies, using the properties of RNA molecules, for analyzing and controlling cell fate in bacteria. He is also the co-founder and president of the first community lab for biotechnology in France "La Paillasse", affiliated with the DIYbio world movement. In 2007, he was one of the founder and participants of the first french iGEM team that became finalist of the competition and received the first prize for foundational research.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/d/d4/Photo_Andrew.jpeg" alt="andrew" align="left" height="150px"/><br />
<h3>Dr. Andrew Tolonen</h3><br />
<i>Group leader at Genoscope</i><br/><br />
<a href="mailto:atolonen@gmail.com">Contact</a><br/><br/><br />
<p><br />
After a PhD in genetics and genomics of cyanobacteria at MIT and a post-doc in the team of George Church at Harvard, one of the largest synthetic biology laboratories in the world, he is now a researcher at Genoscope in Evry. His work focuses on the manufacture of biofuels by cyanobacteria. Andrew Tolonen attends the our team in the selection and construction of the project. He participated in the supervision of Harvard iGEM teams during his post-PhD.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/d/d8/Photo_Nicolas.jpg" alt="nicolas" align="left" height="150px"/><br />
<h3>Dr. Nicolas Pollet</h3><br />
<i>Group leader of the Metamorphosis team at iSSB</i><br/><br />
<a href="mailto:nicolas.pollet@issb.genopole.fr">Contact</a><br/><br/><br />
<p><br />
After a PhD in developmental physiology at INSERM, he was a Marie Curie post-doctoral fellow at the German Cancer Research Institute in Heidelberg. He is now a CNRS senior scientist, head of the Metamorphosys group at iSSB working in genomics and systems biology using the Xenopus frog model. Nicolas Pollet will participate to IGEM as supervisor for the first time this year.<br />
</p><br />
</td><br />
</tr><br />
</table><br />
<br />
<h1>Advisors</h1><br />
<br />
<table id="team" cellspacing="10"><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/6b/Photo_Anna.jpeg" alt="ania" align="left" height="150px"/><br />
<h3>Anna Młynarczyk</h3><br />
<i>Doctoral student in proteomics at the university of Evry</i><br/><br/><br />
<p><br />
She obtained a Master in Biotechnology at the Westpomeranian University of Technology in Szczecin in Poland. During her university studies she has conducted several training courses abroad (UEVE, TEPAK Cyprus) and in Poland which made her want to do research. Anna also performs the tutoring, which allows her to develop teaching skills.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/f/fa/Aurore.png" alt="aurore" align="left" height="150px"/><br />
<h3>Dr. Aurore THELIE</h3><br />
<i>PostDoc at Dr. Pollet's lab</i><br/><br/><br />
<p><br />
After a molecular biology training and a PhD in Reproductive Biology at INRA, she was a post-doctoral fellow at the Institute of Molecular Biology and Medicine (IBMM) in Belgium where she discovered the Xenopus model and all opportunity of study it offers. In 2012 she joined the Metamorphosys group at iSSB to study motoneurons and construct Xenopus transgenic lines using synthetic biology tools.<br />
</p><br />
</td><br />
</tr><br />
</table><br />
<br />
<script type="text/javascript">writeFooter()</script><br />
</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/TeamTeam:Evry/Team2012-10-27T03:54:45Z<p>Cyrpaut: </p>
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<img src="https://static.igem.org/mediawiki/2012/f/f8/Schools.gif" alt="Gif_Evry" width="380" height ="380" style="float: right;"/><br />
<br />
<br/><br />
<h2>The members of the team come from different schools and universities:</h2><br />
<ul><br />
<li><a href="http://www.issb.genopole.fr/Education">Evry university, Master mSSB </a>(Synthetic and systemic biology)</li><br />
<li><a href="http://www.uvsq.fr/welcome-to-uvsq-207282.kjsp?RH=ACCUEIL-FR&RF=ACCUEIL-EN">Versailles Saint Quentin university</a>(Biology)</li><br />
<li><a href="http://www.univ-paris1.fr/">Paris 1 Pantheon-Sorbonne University </a>(Philosophy)</li><br />
<li><a href="http://www.ens.fr/?lang=en">Ecole Normale Supérieure</a>(Engineering)</li><br />
<li><a href="http://www.ecp.fr/lang/en/homepage">Ecole Centrale Paris </a>(Engineering)</li><br />
<li><a href="http://www.supbiotech.fr/en/presentation-supbiotech.aspx">Sup'Biotech Paris </a>(Biotechnology)</li><br />
<li><a href="http://www.esiee.fr/en/">ESIEE Management </a>(Biotechnology)</li><br />
<li><a href="http://www.telecom-sudparis.eu/en_accueil.html>Télécom Sud Paris )</a>(Telecommunication Engineering & Managment</li><br />
<li><a href="http://www.epita.fr/masters/">Epita </a>(Computer Science)</li><br />
</ul><br />
<br />
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<center><h1><a name="envi" style="text-decoration:none; color: white;">Our environment: a key location for synthetic biology in France</a></h1></center><br />
<br />
<h2>Environment</h2><br />
<br />
<br />
<a href="http://www.issb.genopole.fr/"><img src="https://static.igem.org/mediawiki/2012/b/bf/Photo_iSSB.jpeg" alt="logo issb" align="left" height="200px"/></a><br />
<br />
<h3>The Institute of Systems and Synthetic Biology (iSSB):</h3><br />
<p><br />
iSSB is located on the Genopole® in Evry, host team in the summer. The iSSB is a laboratory at the University of Evry and CNRS, supported by Genopole®. It is a multidisciplinary environment where collaborate physicists, chemists, computer scientists and biologists. It is also the laboratory who founded and directs the Master 2 Systems Biology Synthetic and MSSB. This master of avant-garde, unique in France, offers courses provided by researchers at the forefront of their field, which guaranteed training in the state of the art techniques used in synthetic biology.<br/><br/><br />
</p><br />
<br><br><br />
<br />
<h2>Collaborations</h2><br />
<br />
<a href="http://www.lapaillasse.org/"><img src="https://static.igem.org/mediawiki/2012/c/c4/Logo_lapaillasse.png" alt="logo la paillasse" align="left" height="200px"/></a><br />
<br />
<h3>La Paillasse: The Paris community Lab for Biotech</h3><br />
<p><br />
La Paillasse is a physical and web platform for citizen scientists, amateur biologists, researchers and entrepreneurs that fosters open-science, debates and hands-on practice of Biotechnology. La Paillasse is also the first and largest community laboratory for Biotech in France. This year, La Paillasse and some of its members are joining the team of Evry to participate in the design and the realization of one of the coolest iGEM project ever: The french froggies. During the summer, La Paillasse have hosted and organized meet-ups between citizens and the iGEM team for explaining the stakes of our iGEM projects and of Synthetic Biology in general. More in the Human practice section. <a href="http://www.lapaillasse.org">La Paillasse website! </a><br />
</p><br />
<br><br><br><br><br />
<br />
<h1>Lab team</h1><br />
<br />
<table id="team" cellspacing="10" ><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/1/19/Photo_Tristan.jpeg" alt="tristan" align="left" height="150px"/><br />
<h3>Tristan Cerisy</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
I did my bachelor at Evry’s University, in Bioinformatics. During that year when Jean-loup Faulon presented iGem and synthetic biology, I found it very interesting. I wanted to participate with iGem from my bachelor. Because no team existed yet close to our university, William and I decided to create this team in November and we looked for interested people, funding and projects. I am very excited to be involved in this project with this wonderful team.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/68/Jorge2.png" alt="jorge" align="left" height="150px"/><br />
<h3>Jorgelindo Da Veiga Moreira</h3><br />
<i>Master 1, AIV, Diderot University</i><br/><br/><br />
<p><br />
I’m a bachelor graduated student from a Parisian engineering school. I look forward to a Master degree in biotechnology. I discovered synthetic biology mainly through conferences and I’ve been immediately fascinated by this new biological approach to work on living systems. iGEM is a good opportunity for me to start in this field and to acquire experience for my future engineering carrier.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/d/d4/Photo_Carolina.jpeg" alt="carolina" align="left" height="150px"/><br />
<h3>Carolina Gallo Lopez</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
Having acquired a background in biology and a first year of master in “Genomics, Cells, Development and Evolution” at the University of Paris Sud 11, I started to be interested in systems biology since my last year of bachelor and decided on getting involved in this approach during both my second year of master and my master’s thesis. I am participating in the iGEM competition as it allows me to combine experimental work with theoretical modelling. I am keen to learn not only different biological techniques and modeling approaches but also to learn from my teammates and other iGEM teams.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/8/8e/Photo_Tiffany.jpeg" alt="tiffany" align="left" height="150px"/><br />
<h3>Tiffany Souterre</h3><br />
<i>5th year, Sup'Biotech Paris</i><br/><br/><br />
<p><br />
After a BTS in Biotechnology, I went to Sup'Biotech, an engineering school in Biotechnology. For 6 years, I have been studying DNA manipulation, bacteria transformation... but it is only recently that I have heard about Synthetic Biology. It is such a promising and interesting field but requires multidisciplinary skills. I am eager to increase my knowledge to be able to work at the interface of biology and computer science. I discovered iGEM thanks to the 2009 Sup'Biotech team and I have no doubt it will be a great opportunity to learn more, gain experience and hopefully bring a modest contribution to Synthetic Biology.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/a/a4/Photo_Will.jpeg" alt="william" align="left" height="150px"/><br />
<h3>William Rostain</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
I did my bachelor in Edinburgh university, and came into contact with<br />
synthetic biology when I participated iGem with the 2010 Edinburgh team. My background is mostly molecular microbiology and biotechnology, but during iGEM I came into contact with modelling and how it could serve biology, thanks to the great modellers in our team. I decided to participate in mSSB in order to learn some more about modelling and programming so I could work more closely with computer people later :)<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/63/Photo_Cyrille.jpeg" alt="Cyrille" align="left" height="150px"/><br />
<h3>Cyrille Pauthenier</h3><br />
<i>Student of the Ecole Normale Supérieure and member of mSSB</i><br/><br/><br />
<p><br />
I participated in the 2011 iGEM Paris-Bettencourt team who was a finalist and won the prize for best presentation at the European semi-final, and then sweet sixteen at the MIT. I've studied this year in the mSSB master, I'm now about to start a PhD in metabolic engineering at Jean-Loup Faulon's laboratory at iSSB.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/5/57/Photo_Karine.jpeg" alt="karine" align="left" height="150px"/><br />
<h3>Karine Chauris</h3><br />
<i>5th year, Sup'Biotech Paris</i><br/><br/><br />
<p><br />
I’m currently in my final year at Sup'Biotech Paris, a school of biotechnology, and my professional aim is to work on bioproduction processes. I’m attracted, since I was young, by all the possibilities of DNA manipulation and discovered synthetic biology with the first synthetic bacteria. Why did I join iGEM? It's a unique chance to participate in a challenging and concrete project.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/e/eb/Jo2.png" alt="joachim" align="left" height="150px"/><br />
<h3>Joachim Eeckhout</h3><br />
<i>4th year, Sup'Biotech Paris</i><br/><br/><br />
<p><br />
I'm in my fourth year at Sup'Biotech, an engeenering school in biotechnologies. Like many others, I have been amazed by the controversy surrounding the publication of Craig Venter and his synthetic bacterium "Synthia". Since then, I am very interested to acquire expertise in this field and the iGEM competition is a chance for me to be part of this new science.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/b/b0/Photo_Raphael.jpeg" alt="raphael" align="left" height="150px"/><br />
<h3>Raphael Ferreira</h3><br />
<i>1st year, AIV</i><br/><br/><br />
<p><br />
I've currently finished my license (Bachelor) in biotechnology and I'm about to join the AIV (Interdisciplinary approaches to life science) Masters. I discovered synthetic biology with the Craig Venter's paper (released in may 2010). After that, my school gave me a 5 month research & information processing project on synthetic biology. Through this project I've discovered the iGEM competition and the opportunities surrounding the emergence of this science filed. Enrolling in an iGEM team will give me a sight of how synthetic biology experimentations are, and also, to work with people who are interested in this science too.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/61/Photo_PierreYves.jpeg" alt="pierre-yves" align="left" height="150px"/><br />
<h3>Pierre Yves Nogue</h3><br />
<i>2nd year Biology degree, Versaille Saint-Quentin University</i><br/><br/><br />
<p><br />
Biologist student in Paris university, I've discovered the synthetic biology when I've joined "La Paillasse", a biohackspace in Paris. In the same time, I've learned about the existence of the Igem competition, and decided to join the Evry team after some meetings. Indeed, iGEM looks for me as a very good opportunity to work on really interesting subjects in an encouraging work atmosphere, and I've found all this advantages in the Evry team!<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/7/70/Photo_Hafez.jpeg" alt="hafez" align="left" height="150px"/><br />
<h3>Hafez El-Sayyed</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
I did my bachelor's degree and my first Master year In Beirut Arab University, Lebanon, In Biochemistry and Molecular Biology. I came across the term synthetic Biology on the mSSB Website and then started doing some research about the Synthetic Biology topic. Now I want to be part of this wave of unorthodox and ingenious attempts to help create better things around us. I COULDN'T BE Prouder <br />
to be part of IGEM EVRY 2012.<br/><br />
</p><br />
</td><br />
</tr><br />
<br />
</table><br />
<br />
<h1>Modeling team</h1><br />
<br />
<table id="team" cellspacing="10"><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/b/b6/Photo_Artemi.jpeg" alt="artémis" align="left" height="150px"/><br />
<h3>Artémis Llamosi</h3><br />
<i>3rd year, Ecole Centrale Paris</i><br/><br/><br />
<p><br />
After completing a Master in engineering at École Centrale Paris and a research master in applied mathematics for biology at Paris 6 University, I am to start a PhD thesis on real-time control of biological systems on microfluidics chips. For many years interested in the relation between maths and biology, I discovered synthetic biology in 2011 through its connection to systems biology and immediately got "infected". My expertise being mostly on theoretical aspects, I enrolled in iGEM to get closer to the wetlab and apply my scholar knowledge to real life problems.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/b/b9/Photo_Pierre.jpeg" alt="pierre" align="left" height="150px"/><br />
<h3>Pierre Parutto</h3><br />
<i>5th year, Epita</i><br/><br/><br />
<p><br />
I am curently in 5th year at the engineering school Epita and<br />
specialized in scientific computations. I am interested in biology<br />
since high school and more especially in the links between biological<br />
systems and my speciality: computer science. After all a cell can be<br />
seen as a kind of computer, as seen in the name "genetic code". I learned about the IGEM comptetition when I discovered the synthetic biology field in an article in Nature a few years ago. Since then I<br />
wanted to participate to the comptetition but never had the<br />
opportunity. I bring to the team my programming and engineering skills and hope to<br />
learn a lot from biologist in the lab.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/2/22/Photo_Iryna.jpeg" alt="iryna" align="left" height="150px"/><br />
<h3>Iryna Nikolayeva</h3><br />
<i>2nd year, Telecom Sud Paris</i><br/><br/><br />
<p><br />
As specialization of my last year in the engineering school Telecom SudParis, I will be doing the mSSB (master in systemic and synthetic biology). I've got useful computer science and maths skills for modeling and making the wiki. I imagine that mixing technologies and life science can lead to exciting inventions and discoveries. The iGEM seemed to me a nice opportunity to get to know the synthetic biology background and interact with students that are interested in it!<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/66/M%26m.jpg" alt="mohamed" align="left" height="150px"/><br />
<h3>Mohamed Machat</h3><br />
<i>Master 2, mSSB, Evry university</i><br/><br/><br />
<p><br />
I am a civil engineer from Tunisia. My penchant for genetics has started since my first biology lectures in college. However, the first opportunity that I got to go into this field showed up in 2011, when I was admissible to join the ISSB master program. So I left my engineer job and have moved to France. The adventure of iGem looks to me a good step towards my career ambition: invest my mathematics and mechanics background into oncology!!<br />
</p><br />
</td><br />
</tr><br />
</table><br />
<br />
<h1>Human practice</h1><br />
<br />
<table><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/a/a5/Photo_Clement.jpeg" alt="clément" align="left" height="150px"/><br />
<h3>Clément Marquet</h3><br />
<i>Master 2, Philosophy, Paris I Pantheon-Sorbonne University</i><br/><br/><br />
<p><br />
I'm completing a master of philosophy of science at Paris 1 Pantheon Sorbonne University. I discovered synthetic biology about two years ago in a popular scientific review and was struck by the ambition of the field and the diversity of disciplines involved in it. I was seduced by the special place that laboratories such as Synberc give to social sciences and started thinking about some epistemological questions that could be raised by Feynman’s word on knowing and making or by the convergence of biology and technology. I saw in the iGEM contest an opportunity to discover scientific work from the inside and to experiment how philosophical reflections could get practical and debated inside a scientific group.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/1/1e/Chassis.jpg" alt="chassis" align="left" width="180px" style="padding:5px;"/><br />
<h3>Chassis</h3><br />
<i>Function: Mascott</i><br/><br/><br />
<p><br />
Hey I just met you! I am chassis, Team Evry's famous mascott! I kind of enjoy synthetic biology since I heard I could serve science and represent <i> Xenopus tropicalis </i> tough condition! In my lost moments I really enjoy French wine, maybe we'll share a bottle or two! See you in Boston! <a href="https://www.facebook.com/chassis.igemevry"> Feel free to contact me on facebook! </a><br />
<br />
<br/><br />
<br/><br />
<br/><br />
<br/><br />
<br/><br />
</p><br />
</td><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/63/Frogandwine.jpg" alt="chassisandwine" align="left" height="250px" width="150px"/><br />
</td><br />
</tr><br />
</table><br />
<br />
<h1>Instructors</h1><br />
<br />
<table id="team" cellspacing="10"><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/68/Photo_Alfonso.jpeg" alt="alfonso" align="left" height="150px"/><br />
<h3>Dr. Alfonso Jaramillo</h3><br />
<i>Group leader of the Synth-Bio team at iSSB</i><br/><br />
<a href="mailto:alfonso.jaramillo@gmail.com">Contact</a><br/><br/><br />
<p><br />
After a PhD in theoretical physics, he was converted to synthetic biology for over 10 years. He gained international recognition in this field. He is now the team director of the Bio-Synth iSSB, working on the design, synthesis and characterization of biological regulatory artificial pathways. He is also one of the main teachers of the mSSB (synthetic and systematic biology master). Alfonso Jaramillo will be our main supervisor. He led Valencia iGEM team in 2006, and participated in the supervision of Valencia's and Paris' teams.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/5/55/Photo_Thomas.jpeg" alt="thomas" align="left" height="150px"/><br />
<h3>Thomas Landrain</h3><br />
<i>PhD candidate in Jaramillo's Group at iSSB. Co-founder and President of La Paillasse</i><br/><br />
<a href="mailto:thomas.landrain@gmail.com">Contact</a><br/><br/><br />
<p><br />
Ex-student of the Ecole Normale Superieure de Paris, he is now a PhD student at iSSB in Alfonso Jaramillo's Group where he is developing new technologies, using the properties of RNA molecules, for analyzing and controlling cell fate in bacteria. He is also the co-founder and president of the first community lab for biotechnology in France "La Paillasse", affiliated with the DIYbio world movement. In 2007, he was one of the founder and participants of the first french iGEM team that became finalist of the competition and received the first prize for foundational research.<br />
</p><br />
</td><br />
</tr><br />
<br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/d/d4/Photo_Andrew.jpeg" alt="andrew" align="left" height="150px"/><br />
<h3>Dr. Andrew Tolonen</h3><br />
<i>Group leader at Genoscope</i><br/><br />
<a href="mailto:atolonen@gmail.com">Contact</a><br/><br/><br />
<p><br />
After a PhD in genetics and genomics of cyanobacteria at MIT and a post-doc in the team of George Church at Harvard, one of the largest synthetic biology laboratories in the world, he is now a researcher at Genoscope in Evry. His work focuses on the manufacture of biofuels by cyanobacteria. Andrew Tolonen attends the our team in the selection and construction of the project. He participated in the supervision of Harvard iGEM teams during his post-PhD.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/d/d8/Photo_Nicolas.jpg" alt="nicolas" align="left" height="150px"/><br />
<h3>Dr. Nicolas Pollet</h3><br />
<i>Group leader of the Metamorphosis team at iSSB</i><br/><br />
<a href="mailto:nicolas.pollet@issb.genopole.fr">Contact</a><br/><br/><br />
<p><br />
After a PhD in developmental physiology at INSERM, he was a Marie Curie post-doctoral fellow at the German Cancer Research Institute in Heidelberg. He is now a CNRS senior scientist, head of the Metamorphosys group at iSSB working in genomics and systems biology using the Xenopus frog model. Nicolas Pollet will participate to IGEM as supervisor for the first time this year.<br />
</p><br />
</td><br />
</tr><br />
</table><br />
<br />
<h1>Advisors</h1><br />
<br />
<table id="team" cellspacing="10"><br />
<tr><br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/6/6b/Photo_Anna.jpeg" alt="ania" align="left" height="150px"/><br />
<h3>Anna Młynarczyk</h3><br />
<i>Doctoral student in proteomics at the university of Evry</i><br/><br/><br />
<p><br />
She obtained a Master in Biotechnology at the Westpomeranian University of Technology in Szczecin in Poland. During her university studies she has conducted several training courses abroad (UEVE, TEPAK Cyprus) and in Poland which made her want to do research. Anna also performs the tutoring, which allows her to develop teaching skills.<br />
</p><br />
</td><br />
<br />
<td><br />
<img src="https://static.igem.org/mediawiki/2012/f/fa/Aurore.png" alt="aurore" align="left" height="150px"/><br />
<h3>Dr. Aurore THELIE</h3><br />
<i>PostDoc at Dr. Pollet's lab</i><br/><br/><br />
<p><br />
After a molecular biology training and a PhD in Reproductive Biology at INRA, she was a post-doctoral fellow at the Institute of Molecular Biology and Medicine (IBMM) in Belgium where she discovered the Xenopus model and all opportunity of study it offers. In 2012 she joined the Metamorphosys group at iSSB to study motoneurons and construct Xenopus transgenic lines using synthetic biology tools.<br />
</p><br />
</td><br />
</tr><br />
</table><br />
<br />
<script type="text/javascript">writeFooter()</script><br />
</html></div>Cyrpauthttp://2012.igem.org/Team:EvryTeam:Evry2012-10-27T03:47:44Z<p>Cyrpaut: </p>
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<h1 align="center">Welcome to the French Froggies Home Page!</h1><br />
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<br />
<p>This year, the Evry iGEM team is bringing synthetic biology to multicellular organisms, opening the era of <em>synthetic physiology</em>. We created the <a href="https://2012.igem.org/Team:Evry/FrenchFrog"><em>first parts for Xenopus</em></a> and an <a href="https://2012.igem.org/Team:Evry/AIDSystem"><em>synthetic orthogonal hormonal</em></a> system to link them together. We also created new tools for <a href="https://2012.igem.org/Team:Evry/Modeling"><em>modeling the tadpole</em></a> physiology, <a href="https://2012.igem.org/Team:Evry/GB"><em>assemble multiple parts</em></a> in a single shot, wrote a <a href="https://2012.igem.org/Team:Evry/FrogForDummies"><em>guidebook</em></a> to help future teams with <i>Xenopus</i> and studied the <a href="https://2012.igem.org/Team:Evry/HumanPractice/Introduction"><em>consequences of the arrival</em></a> of vertebrates in iGEM.</p><br />
<br />
<p>You can have a look at our <a href="https://2012.igem.org/Team:Evry/Achievements"><em>achievements page</em></a> or visit the universe of multicellular synthetic biology by clicking on the galaxy below !</p><br />
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<img src="https://static.igem.org/mediawiki/2012/5/51/Sponsors_banner.png" width=900px align="center"><br />
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</html></div>Cyrpauthttp://2012.igem.org/File:Sponsors_banner.pngFile:Sponsors banner.png2012-10-27T03:47:02Z<p>Cyrpaut: uploaded a new version of &quot;File:Sponsors banner.png&quot;</p>
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<div></div>Cyrpauthttp://2012.igem.org/File:Sponsors_banner.pngFile:Sponsors banner.png2012-10-27T03:45:34Z<p>Cyrpaut: uploaded a new version of &quot;File:Sponsors banner.png&quot;</p>
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<div></div>Cyrpauthttp://2012.igem.org/Team:EvryTeam:Evry2012-10-27T03:38:51Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
<head><br />
<title>Welcome ot iGEM Evry's Wiki!</title><br />
<link media="screen" rel="stylesheet" href="https://2012.igem.org/Team:Evry/colorbox.css?action=raw&ctype=text/css" /><br />
<br />
<script src="http://code.jquery.com/jquery-latest.min.js" type="text/javascript"></script><br />
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<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/jquery.colorbox-min.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
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<!--<br />
<script type="text/javascript"><br />
$(function()<br />
{<br />
$(window).bind('load', <br />
function(e) <br />
{<br />
$.colorbox({opacity:0.3, href:"https://2012.igem.org/Team:Evry/testpopup2?action=raw&ctype=text/html"}); <br />
});<br />
});<br />
</script><br />
--><br />
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</head><br />
<br />
<br><br />
<br />
<h1 align="center">Welcome to the French Froggies Home Page!</h1><br />
<br />
<br />
<p>This year, the Evry iGEM team is bringing synthetic biology to multicellular organisms, opening the era of <em>synthetic physiology</em>. We created the <a href="https://2012.igem.org/Team:Evry/FrenchFrog"><em>first parts for Xenopus</em></a> and an <a href="https://2012.igem.org/Team:Evry/AIDSystem"><em>synthetic orthogonal hormonal</em></a> system to link them together. We also created new tools for <a href="https://2012.igem.org/Team:Evry/Modeling"><em>modeling the tadpole</em></a> physiology, <a href="https://2012.igem.org/Team:Evry/GB"><em>assemble multiple parts</em></a> in a single shot, wrote a <a href="https://2012.igem.org/Team:Evry/FrogForDummies"><em>guidebook</em></a> to help future teams with <i>Xenopus</i> and studied the <a href="https://2012.igem.org/Team:Evry/HumanPractice/Introduction"><em>consequences of the arrival</em></a> of vertebrates in iGEM.</p><br />
<br />
<p>You can have a look at our <a href="https://2012.igem.org/Team:Evry/Achievements"><em>achievements page</em></a> or visit the universe of multicellular synthetic biology by clicking on the galaxy below !</p><br />
</div><br />
<div style="position:absolute;top:370px;right:-8px;"><br />
<canvas id="sitemap" width="1000" height="600"></canvas><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript" src="https://2012.igem.org/Team:Evry/arbor.js?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-tween.js"></script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-graphics.js"></script><br />
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document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/main-img.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
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<div style="position:absolute; top: 968px; background: #ffffff;width: 1000px;height: 90px;left: -20px;color: black;-moz-border-radius-bottomright: 15px;-webkit-border-bottom-left-radius: 15px;-moz-border-radius-bottomleft: 15px;-webkit-border-bottom-right-radius: 15px; padding-top:10px"><br />
<img src="https://static.igem.org/mediawiki/2012/5/51/Sponsors_banner.png" width=900px align="center"><br />
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</html></div>Cyrpauthttp://2012.igem.org/Team:EvryTeam:Evry2012-10-27T03:38:19Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
<head><br />
<title>Welcome ot iGEM Evry's Wiki!</title><br />
<link media="screen" rel="stylesheet" href="https://2012.igem.org/Team:Evry/colorbox.css?action=raw&ctype=text/css" /><br />
<br />
<script src="http://code.jquery.com/jquery-latest.min.js" type="text/javascript"></script><br />
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<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/jquery.colorbox-min.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
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<!--<br />
<script type="text/javascript"><br />
$(function()<br />
{<br />
$(window).bind('load', <br />
function(e) <br />
{<br />
$.colorbox({opacity:0.3, href:"https://2012.igem.org/Team:Evry/testpopup2?action=raw&ctype=text/html"}); <br />
});<br />
});<br />
</script><br />
--><br />
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</head><br />
<br />
<br><br />
<br />
<h1 align="center">Welcome to the French Froggies Home Page!</h1><br />
<br />
<br />
<p>This year, the Evry iGEM team is bringing synthetic biology to multicellular organisms, opening the era of <em>synthetic physiology</em>. We created the <a href="https://2012.igem.org/Team:Evry/FrenchFrog"><em>first parts for Xenopus</em></a> and an <a href="https://2012.igem.org/Team:Evry/AIDSystem"><em>synthetic orthogonal hormonal</em></a> system to link them together. We also created new tools for <a href="https://2012.igem.org/Team:Evry/Modeling"><em>modeling the tadpole</em></a> physiology, <a href="https://2012.igem.org/Team:Evry/GB"><em>assemble multiple parts</em></a> in a single shot, wrote a <a href="https://2012.igem.org/Team:Evry/FrogForDummies"><em>guidebook</em></a> to help future teams with <i>Xenopus</i> and studied the <a href="https://2012.igem.org/Team:Evry/HumanPractice/Introduction"><em>consequences of the arrival</em></a> of vertebrates in iGEM.</p><br />
<br />
<p>You can have a look at our <a href="https://2012.igem.org/Team:Evry/Achievements">achievements page</a> or visit the universe of multicellular synthetic biology by clicking on the galaxy below !</p><br />
</div><br />
<div style="position:absolute;top:370px;right:-8px;"><br />
<canvas id="sitemap" width="1000" height="600"></canvas><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript" src="https://2012.igem.org/Team:Evry/arbor.js?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-tween.js"></script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-graphics.js"></script><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/main-img.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
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</div><br />
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<div style="position:absolute; top: 968px; background: #ffffff;width: 1000px;height: 90px;left: -20px;color: black;-moz-border-radius-bottomright: 15px;-webkit-border-bottom-left-radius: 15px;-moz-border-radius-bottomleft: 15px;-webkit-border-bottom-right-radius: 15px; padding-top:10px"><br />
<img src="https://static.igem.org/mediawiki/2012/5/51/Sponsors_banner.png" width=900px align="center"><br />
<br />
<br />
</html></div>Cyrpauthttp://2012.igem.org/Team:EvryTeam:Evry2012-10-27T03:37:28Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
<head><br />
<title>Welcome ot iGEM Evry's Wiki!</title><br />
<link media="screen" rel="stylesheet" href="https://2012.igem.org/Team:Evry/colorbox.css?action=raw&ctype=text/css" /><br />
<br />
<script src="http://code.jquery.com/jquery-latest.min.js" type="text/javascript"></script><br />
<br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/jquery.colorbox-min.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
<br />
<!--<br />
<script type="text/javascript"><br />
$(function()<br />
{<br />
$(window).bind('load', <br />
function(e) <br />
{<br />
$.colorbox({opacity:0.3, href:"https://2012.igem.org/Team:Evry/testpopup2?action=raw&ctype=text/html"}); <br />
});<br />
});<br />
</script><br />
--><br />
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</head><br />
<br />
<br><br />
<br />
<h1 align="center">Welcome to the French Froggies Home Page!</h1><br />
<br />
<br />
<p>This year, the Evry iGEM team is bringing synthetic biology to multicellular organisms, opening the era of <em>synthetic physiology</em>. We created the <a href="https://2012.igem.org/Team:Evry/FrenchFrog"><em>first parts for Xenopus</em></em> and an <a href="https://2012.igem.org/Team:Evry/AIDSystem"><em>synthetic orthogonal hormonal</em></a> system to link them together. We also created new tools for <a href="https://2012.igem.org/Team:Evry/Modeling"><em>modeling the tadpole</em></a> physiology, <a href="https://2012.igem.org/Team:Evry/GB"><em>assemble multiple parts</em></a> in a single shot, wrote a <a href="https://2012.igem.org/Team:Evry/FrogForDummies"><em>guidebook</em></a> to help future teams with <i>Xenopus</i> and studied the <a href="https://2012.igem.org/Team:Evry/HumanPractice/Introduction"><em>consequences of the arrival</em></a> of vertebrates in iGEM.</p><br />
<br />
<p>You can have a look at our <a href="https://2012.igem.org/Team:Evry/Achievements">achievements page</a> or visit the universe of multicellular synthetic biology by clicking on the galaxy below !</p><br />
</div><br />
<div style="position:absolute;top:370px;right:-8px;"><br />
<canvas id="sitemap" width="1000" height="600"></canvas><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript" src="https://2012.igem.org/Team:Evry/arbor.js?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-tween.js"></script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-graphics.js"></script><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/main-img.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
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</div><br />
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<div style="position:absolute; top: 968px; background: #ffffff;width: 1000px;height: 90px;left: -20px;color: black;-moz-border-radius-bottomright: 15px;-webkit-border-bottom-left-radius: 15px;-moz-border-radius-bottomleft: 15px;-webkit-border-bottom-right-radius: 15px; padding-top:10px"><br />
<img src="https://static.igem.org/mediawiki/2012/5/51/Sponsors_banner.png" width=900px align="center"><br />
<br />
<br />
</html></div>Cyrpauthttp://2012.igem.org/Team:EvryTeam:Evry2012-10-27T03:33:31Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
<head><br />
<title>Welcome ot iGEM Evry's Wiki!</title><br />
<link media="screen" rel="stylesheet" href="https://2012.igem.org/Team:Evry/colorbox.css?action=raw&ctype=text/css" /><br />
<br />
<script src="http://code.jquery.com/jquery-latest.min.js" type="text/javascript"></script><br />
<br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/jquery.colorbox-min.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
<br />
<!--<br />
<script type="text/javascript"><br />
$(function()<br />
{<br />
$(window).bind('load', <br />
function(e) <br />
{<br />
$.colorbox({opacity:0.3, href:"https://2012.igem.org/Team:Evry/testpopup2?action=raw&ctype=text/html"}); <br />
});<br />
});<br />
</script><br />
--><br />
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</head><br />
<br />
<br><br />
<br />
<h1 align="center">Welcome to the French Froggies Home Page!</h1><br />
<br />
<br />
<p>This year, the Evry iGEM team is bringing synthetic biology to multicellular organisms, opening the era of <em>synthetic physiology</em>. We created the <em>first parts for Xenopus</em>, and an <em>synthetic orthogonal hormonal</em> system to link them together. We also created new tools for <a href="https://2012.igem.org/Team:Evry/Modeling"><em>modeling the tadpole</em></a> physiology, <em>assemble multiple parts</em> in a single shot, wrote a <em>guidebook</em> to help future teams with <i>Xenopus</i> and studied the <em>consequences of the arrival</em> of vertebrates in iGEM.</p><br />
<br />
<p>You can have a look at our <a href="https://2012.igem.org/Team:Evry/Achievements">achievements page</a> or visit the universe of multicellular synthetic biology by clicking on the galaxy below !</p><br />
</div><br />
<div style="position:absolute;top:370px;right:-8px;"><br />
<canvas id="sitemap" width="1000" height="600"></canvas><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript" src="https://2012.igem.org/Team:Evry/arbor.js?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-tween.js"></script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-graphics.js"></script><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/main-img.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
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</div><br />
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<div style="position:absolute; top: 968px; background: #ffffff;width: 1000px;height: 90px;left: -20px;color: black;-moz-border-radius-bottomright: 15px;-webkit-border-bottom-left-radius: 15px;-moz-border-radius-bottomleft: 15px;-webkit-border-bottom-right-radius: 15px; padding-top:10px"><br />
<img src="https://static.igem.org/mediawiki/2012/5/51/Sponsors_banner.png" width=900px align="center"><br />
<br />
<br />
</html></div>Cyrpauthttp://2012.igem.org/Team:EvryTeam:Evry2012-10-27T03:31:38Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
<head><br />
<title>Welcome ot iGEM Evry's Wiki!</title><br />
<link media="screen" rel="stylesheet" href="https://2012.igem.org/Team:Evry/colorbox.css?action=raw&ctype=text/css" /><br />
<br />
<script src="http://code.jquery.com/jquery-latest.min.js" type="text/javascript"></script><br />
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<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/jquery.colorbox-min.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
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<!--<br />
<script type="text/javascript"><br />
$(function()<br />
{<br />
$(window).bind('load', <br />
function(e) <br />
{<br />
$.colorbox({opacity:0.3, href:"https://2012.igem.org/Team:Evry/testpopup2?action=raw&ctype=text/html"}); <br />
});<br />
});<br />
</script><br />
--><br />
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</head><br />
<br />
<br><br />
<br />
<h1 align="center">Welcome to the French Froggies Home Page!</h1><br />
<br />
<br />
<p>This year, the Evry iGEM team is bringing synthetic biology to multicellular organisms, opening the era of <em>synthetic physiology</em>. We created the <em>first parts for Xenopus</em>, and an <em>synthetic orthogonal hormonal</em> system to link them together. We also created new tools for <em>modeling the tadpole</em> physiology, <em>assemble multiple parts</em> in a single shot, wrote a <em>guidebook</em> to help future teams with <i>Xenopus</i> and studied the <em>consequences of the arrival</em> of vertebrates in iGEM.</p><br />
<br />
<p>You can have a look at our <a href="https://2012.igem.org/Team:Evry/Achievements">achievements page</a> or visit the universe of multicellular synthetic biology by clicking on the galaxy below !</p><br />
</div><br />
<div style="position:absolute;top:370px;right:-8px;"><br />
<canvas id="sitemap" width="1000" height="600"></canvas><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript" src="https://2012.igem.org/Team:Evry/arbor.js?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-tween.js"></script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-graphics.js"></script><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/main-img.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
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</div><br />
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<div style="position:absolute; top: 968px; background: #ffffff;width: 1000px;height: 90px;left: -20px;color: black;-moz-border-radius-bottomright: 15px;-webkit-border-bottom-left-radius: 15px;-moz-border-radius-bottomleft: 15px;-webkit-border-bottom-right-radius: 15px; padding-top:10px"><br />
<img src="https://static.igem.org/mediawiki/2012/5/51/Sponsors_banner.png" width=900px align="center"><br />
<br />
<br />
</html></div>Cyrpauthttp://2012.igem.org/Team:EvryTeam:Evry2012-10-27T03:30:50Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
<head><br />
<title>Welcome ot iGEM Evry's Wiki!</title><br />
<link media="screen" rel="stylesheet" href="https://2012.igem.org/Team:Evry/colorbox.css?action=raw&ctype=text/css" /><br />
<br />
<script src="http://code.jquery.com/jquery-latest.min.js" type="text/javascript"></script><br />
<br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/jquery.colorbox-min.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
<br />
<!--<br />
<script type="text/javascript"><br />
$(function()<br />
{<br />
$(window).bind('load', <br />
function(e) <br />
{<br />
$.colorbox({opacity:0.3, href:"https://2012.igem.org/Team:Evry/testpopup2?action=raw&ctype=text/html"}); <br />
});<br />
});<br />
</script><br />
--><br />
<br />
</head><br />
<br />
<br><br />
<br />
<h1 align="center">Welcome to the French Froggies Home Page!</h1><br />
<br />
<br />
<p>This year, the Evry iGEM team is bringing synthetic biology to multicellular organisms, opening the era of <em>synthetic physiology</em>. We created the <em>first parts for Xenopus</em>, and an <em>synthetic orthogonal hormonal</em> system to link them together. We also created new tools for <em>modeling the tadpole</em> physiology, <em>assemble multiple parts</em> in a single shot, wrote a <em>guidebook</em> to help future teams and studied the <em>consequences of the arrival</em> of vertebrates in iGEM.</p><br />
<br />
<p>You can have a look at our <a href="https://2012.igem.org/Team:Evry/Achievements">achievements page</a> or visit the universe of multicellular synthetic biology by clicking on the galaxy below !</p><br />
</div><br />
<div style="position:absolute;top:370px;right:-8px;"><br />
<canvas id="sitemap" width="1000" height="600"></canvas><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript" src="https://2012.igem.org/Team:Evry/arbor.js?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-tween.js"></script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-graphics.js"></script><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/main-img.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
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</div><br />
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<div style="position:absolute; top: 968px; background: #ffffff;width: 1000px;height: 90px;left: -20px;color: black;-moz-border-radius-bottomright: 15px;-webkit-border-bottom-left-radius: 15px;-moz-border-radius-bottomleft: 15px;-webkit-border-bottom-right-radius: 15px; padding-top:10px"><br />
<img src="https://static.igem.org/mediawiki/2012/5/51/Sponsors_banner.png" width=900px align="center"><br />
<br />
<br />
</html></div>Cyrpauthttp://2012.igem.org/Team:EvryTeam:Evry2012-10-27T03:30:13Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
<head><br />
<title>Welcome ot iGEM Evry's Wiki!</title><br />
<link media="screen" rel="stylesheet" href="https://2012.igem.org/Team:Evry/colorbox.css?action=raw&ctype=text/css" /><br />
<br />
<script src="http://code.jquery.com/jquery-latest.min.js" type="text/javascript"></script><br />
<br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/jquery.colorbox-min.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
<br />
<!--<br />
<script type="text/javascript"><br />
$(function()<br />
{<br />
$(window).bind('load', <br />
function(e) <br />
{<br />
$.colorbox({opacity:0.3, href:"https://2012.igem.org/Team:Evry/testpopup2?action=raw&ctype=text/html"}); <br />
});<br />
});<br />
</script><br />
--><br />
<br />
</head><br />
<br />
<br><br />
<br />
<h1 align="center">Welcome to the French Froggies Home Page!</h1><br />
<br />
<br />
<p>This year, the Evry iGEM team is bringing synthetic biology to multicellular organisms, opening the era of <em>synthetic physiology</em>. We created the <em>first parts for Xenopus</em>, and an <em>synthetic orthogonal hormonal</em> system to link them together. We also created new tools for <em>modeling the tadpole</em> physiology, <em>assemble multiple parts</em> in a single shot, wrote a <em>guidebook</em> to help future teams working with iGEM and studied the <em>consequences of the arrival</em> of vertebrates in iGEM.</p><br />
<br />
<p>You can have a look at our <a href="https://2012.igem.org/Team:Evry/Achievements">achievements page</a> or visit the universe of multicellular synthetic biology by clicking on the galaxy below !</p><br />
</div><br />
<div style="position:absolute;top:370px;right:-8px;"><br />
<canvas id="sitemap" width="1000" height="600"></canvas><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript" src="https://2012.igem.org/Team:Evry/arbor.js?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-tween.js"></script><br />
<script type="text/javascript" src="http://arborjs.org/js/lib/arbor-graphics.js"></script><br />
<script type="text/javascript"><br />
document.write('<script type="text/javascript"' + 'src="https://2012.igem.org/Team:Evry/main-img.js' + '?action=raw&ctype=text/javascript">'+'</script'+'>');<br />
</script><br />
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</div><br />
<br />
<div style="position:absolute; top: 968px; background: #ffffff;width: 1000px;height: 90px;left: -20px;color: black;-moz-border-radius-bottomright: 15px;-webkit-border-bottom-left-radius: 15px;-moz-border-radius-bottomleft: 15px;-webkit-border-bottom-right-radius: 15px; padding-top:10px"><br />
<img src="https://static.igem.org/mediawiki/2012/5/51/Sponsors_banner.png" width=900px align="center"><br />
<br />
<br />
</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/FrogForDummiesTeam:Evry/FrogForDummies2012-10-27T03:24:09Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
<h1>Xenopus for dummies: an iGEMer guidebook</h1><br />
<br />
<table><br />
<tr><br />
<td><br />
<br />
<p>Hi iGEMer! Are you brainstorming on a new project? Do you plan to work collaborate with Xenopus? You are at the good place, this book is dedicated for you.</p><br />
<br />
<p>We are the 2012 Evry iGEM team, and this year, we have made the first iGEM project on Xenopus and created the first biobricks to work with. In this small book, we wanted to share with you our experience with this animal, try to give you guidelines for designing your own project and give you references to the resources you can use.</p><br />
<br />
<br /><br />
<br /><br />
<br />
<p><b>You can download it in the PDF format!</b></p><br />
<br />
<br /><br />
<br /><br />
<br />
<div id="contourmenu" class="moredetails" style="margin-left:100px;width:420px;"><br />
<br />
<table style="background:transparent;"><br />
<tr><td><img style="padding-top:10px;padding-left:10px;" src="https://static.igem.org/mediawiki/2012/6/6b/Pdf.png" width=80px></td><br />
<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://static.igem.org/mediawiki/2012/6/60/Xenopusfordummies_-_Final_-_Evry.pdf"><center>Download Xenopus For Dummies!</center></a><br />
<br />
</u></h3></td><br />
</tr></table><br />
<br /><br />
</font><br />
</div><br />
<br />
</td><br />
<br />
<td>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</td><br />
<br />
<td><img align="center" src="https://static.igem.org/mediawiki/2012/8/86/FrogForDummies_cover.png" alt="XenopusForDumies" width="300px" /></td><br />
<br />
</tr></table><br />
<br />
<script type="text/javascript">writeFooter()</script><br />
<br />
</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/SponsorsTeam:Evry/Sponsors2012-10-27T02:48:19Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
<center><br />
<h1>Many thanks to our generous sponsors !</h1><br />
<table><br />
<tr><br />
<td> <a href="http://www.genopole.fr/"><img src="https://static.igem.org/mediawiki/2012/5/58/Logo_genopole.jpg" style="width:100px"></a><br />
<td> <a href="http://www.univ-evry.fr/fr/l_universite/composantes/ufr_sciences_fondamentales_appliquees/departement_de_biologie.html"><img src="https://static.igem.org/mediawiki/2012/5/5a/Logo_univ_bio.png" style="width:150px"></a><br />
<td> <a href="http://www.sanofi.com/"><img src="https://static.igem.org/mediawiki/2012/5/5f/Sanofi_logo.png" style="width:150px"></a><br />
<td> <a href="http://www.issb.genopole.fr/"><img src="https://static.igem.org/mediawiki/2012/6/62/Issb-logo-petit.png" style="width:200px"></a><br />
<tr><br />
<td> <a href="http://www.supbiotech.fr/"><img src="https://static.igem.org/mediawiki/2012/a/aa/LogosupBiotech.jpg" style="width:100px"></a><br />
<td> <a href="http://ambafrance-us.org"><img src="https://static.igem.org/mediawiki/2012/3/3c/Logo_ambassade.png" style="width:250px"></a><br />
<td> <a href="http://www.geneious.com"><img src="https://static.igem.org/mediawiki/2012/5/5e/Logo_geneious.gif" style="width:200px"></a><br />
<td> <a href="http://www.neb.com/nebecomm/default.asp"><img src="https://static.igem.org/mediawiki/2012/1/17/Logo_neb.jpg" style="width:200px"></a><br />
<tr> <br />
<td> <a href="http://www.lambe.univ-evry.fr/"><img src="https://static.igem.org/mediawiki/2012/thumb/f/f4/LAMPE_labo_ania.png/800px-LAMPE_labo_ania.png" style="width:200px"></a><br />
<td> <a href="www.thermofisher.com/"><img src="https://static.igem.org/mediawiki/2012/thumb/6/6c/ThermoFisherSC.png/800px-ThermoFisherSC.png" style="width:200px"></a><br />
<td> <a href="http://www.corning.com"><img src="https://static.igem.org/mediawiki/2012/9/9f/CORNING_%28Orange%29.jpg" style="width:200px"></a><br />
<td> <a href="http://www.idt.com/"><img src="https://static.igem.org/mediawiki/2012/e/e4/Logo_idt.jpg" style="width:200px"></a><br />
<tr><br />
<td><br />
<td><a href="http://www.mathworks.com/"><img src="https://static.igem.org/mediawiki/2012/3/3f/MathWorks_logo.png" style="width:200px"></a><br />
<td><a href="http://www.multichannelsystems.com"><img src="http://www.kuoyang.com.tw/Download/Sale/mcs_logo.jpg" style="width:200px"></a><br />
</table><br />
<br />
<script type="text/javascript">writeFooter()</script><br />
<br />
</body><br />
</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/main-img.jsTeam:Evry/main-img.js2012-10-27T02:44:42Z<p>Cyrpaut: </p>
<hr />
<div>(function($){<br />
<br />
var Renderer = function(elt){<br />
var dom = $(elt)<br />
var canvas = dom.get(0)<br />
var ctx = canvas.getContext("2d");<br />
var gfx = arbor.Graphics(canvas)<br />
var sys = null<br />
<br />
var _vignette = null<br />
var selected = null,<br />
nearest = null,<br />
_mouseP = null;<br />
<br />
<br />
var that = {<br />
init:function(pSystem){<br />
sys = pSystem<br />
sys.screen({size:{width:dom.width(), height:dom.height()},padding:[36,60,36,60]})<br />
<br />
$(window).resize(that.resize)<br />
that.resize()<br />
that._initMouseHandling()<br />
<br />
if (document.referrer.match(/GoldenBricksxxxxxxxx|Plasmids|AIDSystem/)){<br />
// if we got here by hitting the back button in one of the Benchwork, <br />
// start with the Benchwork section pre-selected<br />
that.switchSection('Benchwork')<br />
}<br />
},<br />
resize:function(){<br />
// canvas.width = .5* $(window).width()<br />
// canvas.height = .5* $(window).height()<br />
sys.screen({size:{width:canvas.width, height:canvas.height}})<br />
_vignette = null<br />
that.redraw()<br />
},<br />
redraw:function(){<br />
gfx.clear()<br />
sys.eachEdge(function(edge, p1, p2){<br />
if (edge.source.data.alpha * edge.target.data.alpha == 0) return<br />
gfx.line(p1, p2, {stroke:"#b2b19d", width:3, alpha:edge.target.data.alpha})<br />
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sys.eachNode(function(node, pt){<br />
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if (node.data.shape=='img'){<br />
var img_elem = new Image();<br />
img_elem.src = node.data.url;<br />
ctx.drawImage(img_elem, pt.x-w/2, pt.y-w/2, w, w); // Redimensionnement de l'image prévue comme tu le souhaitais !<br />
gfx.oval(pt.x-w/2, pt.y-w/2, w, w, {fill:node.data.color, alpha:0})<br />
}<br />
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else if (node.data.shape=='dot'){<br />
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gfx.text(node.name, pt.x, pt.y+7, {color:"white", align:"center", font:"Arial", weight:"bold", size:12}) <br />
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gfx.rect(pt.x-w/2, pt.y-8, w, 20, 4, {fill:node.data.color, alpha:node.data.alpha})<br />
gfx.text(node.name, pt.x, pt.y+9, {color:"white", align:"center", font:"Arial", size:12})<br />
gfx.text(node.name, pt.x, pt.y+9, {color:"white", align:"center", font:"Arial", size:12})<br />
}<br />
})<br />
that._drawVignette()<br />
},<br />
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_drawVignette:function(){<br />
var w = canvas.width<br />
var h = canvas.height<br />
var r = 20<br />
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if (!_vignette){<br />
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top.addColorStop(0, "#e0e0e0")<br />
top.addColorStop(.7, "rgba(255,255,255,0)")<br />
<br />
var bot = ctx.createLinearGradient(0,h-r,0,h)<br />
bot.addColorStop(0, "rgba(255,255,255,0)")<br />
bot.addColorStop(1, "white")<br />
<br />
_vignette = {bot:bot}<br />
}<br />
<br />
// top<br />
ctx.fillStyle = _vignette.top<br />
ctx.fillRect(0,0, w,r)<br />
<br />
// bot<br />
ctx.fillStyle = _vignette.bot<br />
ctx.fillRect(0,h-r, w,r)<br />
},<br />
<br />
switchMode:function(e){<br />
if (e.mode=='hidden'){<br />
dom.stop(true).fadeTo(e.dt,0, function(){<br />
if (sys) sys.stop()<br />
$(this).hide()<br />
})<br />
}else if (e.mode=='visible'){<br />
dom.stop(true).css('opacity',0).show().fadeTo(e.dt,1,function(){<br />
that.resize()<br />
})<br />
if (sys) sys.start()<br />
}<br />
},<br />
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switchSection:function(newSection){<br />
var parent = sys.getEdgesFrom(newSection)[0].source<br />
var children = $.map(sys.getEdgesFrom(newSection), function(edge){<br />
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<br />
sys.eachNode(function(node){<br />
if (node.data.shape=='dot') return // skip all but leafnodes<br />
if (node.data.shape=='img') return // skip all but leafnodes<br />
var nowVisible = ($.inArray(node, children)>=0)<br />
var newAlpha = (nowVisible) ? 1 : 0<br />
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sys.tweenNode(node, dt, {alpha:newAlpha})<br />
<br />
if (newAlpha==1){<br />
node.p.x = parent.p.x + .05*Math.random() - .025<br />
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node.tempMass = .001<br />
}<br />
})<br />
},<br />
<br />
<br />
_initMouseHandling:function(){<br />
// no-nonsense drag and drop (thanks springy.js)<br />
selected = null;<br />
nearest = null;<br />
var dragged = null;<br />
var oldmass = 1<br />
<br />
var _section = null<br />
<br />
var handler = {<br />
moved:function(e){<br />
var pos = $(canvas).offset();<br />
_mouseP = arbor.Point(e.pageX-pos.left, e.pageY-pos.top)<br />
nearest = sys.nearest(_mouseP);<br />
<br />
if (!nearest.node) return false<br />
<br />
// if (nearest.node.data.shape!='dot'){<br />
selected = (nearest.distance < 50) ? nearest : null<br />
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dom.addClass('linkable')<br />
window.status = selected.node.data.link//.replace(/^\//,"http://"+window.location.host+"/")//.replace(/^#/,'')<br />
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dom.removeClass('linkable')<br />
window.status = ''<br />
}<br />
// }else <br />
if ($.inArray(nearest.node.name, ['The French Froggies Project!','Xenopus as a chassis','Hormonal Communicati','Modelingxxxxxxxxxxxx','GoldenBricksxxxxxxxx', 'Human Practicexxxxxx', 'The Teamxxxxxxxxxxxx']) >=0 ){<br />
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_section = nearest.node.name<br />
that.switchSection(_section)<br />
}<br />
dom.removeClass('linkable')<br />
window.status = ''<br />
}<br />
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$(canvas).unbind('mousemove', handler.moved);<br />
$(canvas).bind('mousemove', handler.dragged)<br />
$(window).bind('mouseup', handler.dropped)<br />
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var old_nearest = nearest && nearest.node._id<br />
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if (dragged===null || dragged.node===undefined) return<br />
if (dragged.node !== null) dragged.node.fixed = false<br />
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// selected = null<br />
$(canvas).unbind('mousemove', handler.dragged)<br />
$(window).unbind('mouseup', handler.dropped)<br />
$(canvas).bind('mousemove', handler.moved);<br />
_mouseP = null<br />
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}<br />
<br />
<br />
}<br />
<br />
$(canvas).mousedown(handler.clicked);<br />
$(canvas).mousemove(handler.moved);<br />
<br />
}<br />
}<br />
<br />
return that<br />
}<br />
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<br />
var Nav = function(elt){<br />
var dom = $(elt)<br />
<br />
var _path = null<br />
<br />
var that = {<br />
init:function(){<br />
$(window).bind('popstate',that.navigate)<br />
dom.find('> a').click(that.back)<br />
$('.more').one('click',that.more)<br />
<br />
$('#Model dl:not(.datastructure) dt').click(that.reveal)<br />
that.update()<br />
return that<br />
},<br />
more:function(e){<br />
$(this).removeAttr('href').addClass('less').html('&nbsp;').siblings().fadeIn()<br />
$(this).next('h2').find('a').one('click', that.less)<br />
<br />
return false<br />
},<br />
less:function(e){<br />
var more = $(this).closest('h2').prev('a')<br />
$(this).closest('h2').prev('a')<br />
.nextAll().fadeOut(function(){<br />
$(more).text('creation & use').removeClass('less').attr('href','#')<br />
})<br />
$(this).closest('h2').prev('a').one('click',that.more)<br />
<br />
return false<br />
},<br />
reveal:function(e){<br />
$(this).next('dd').fadeToggle('fast')<br />
return false<br />
},<br />
back:function(){<br />
_path = "/"<br />
if (window.history && window.history.pushState){<br />
window.history.pushState({path:_path}, "", _path);<br />
}<br />
that.update()<br />
return false<br />
},<br />
navigate:function(e){<br />
var oldpath = _path<br />
if (e.type=='navigate'){<br />
_path = e.path<br />
if (window.history && window.history.pushState){<br />
window.history.pushState({path:_path}, "", _path);<br />
}else{<br />
that.update()<br />
}<br />
}else if (e.type=='popstate'){<br />
var state = e.originalEvent.state || {}<br />
_path = state.path || window.location.pathname.replace(/^\//,'')<br />
}<br />
if (_path != oldpath) that.update()<br />
},<br />
update:function(){<br />
var dt = 'slow'<br />
if (_path===null){<br />
// this is the original page load. don't animate anything just jump<br />
// to the proper state<br />
_path = window.location.pathname.replace(/^\//,'')<br />
dt = 0<br />
dom.find('p').css('opacity',0).show().fadeTo('slow',1)<br />
}<br />
<br />
switch (_path){<br />
case '':<br />
case '/':<br />
// dom.find('p').text('a graph visualization library using web workers and jQuery')<br />
dom.find('> a').removeClass('active').attr('href','#')<br />
<br />
$('#Model').fadeTo('fast',0, function(){<br />
$(this).hide()<br />
$(that).trigger({type:'mode', mode:'visible', dt:dt})<br />
})<br />
document.title = "The French Froggies Project!"<br />
break<br />
<br />
}<br />
<br />
}<br />
}<br />
return that<br />
}<br />
<br />
$(document).ready(function(){<br />
var CLR = {<br />
branch:"#b2b19d",<br />
level3benchwork:"#FFBF3E",<br />
level3model:"#4B86C7"<br />
}<br />
/*Thibault BEGIN*/<br />
var theUI = {<br />
<br />
// ['The French Froggies Project!','Xenopus as a chassis','Hormonal Communicati','Modelingxxxxxxxxxxxx','GoldenBricksxxxxxxxx', 'Human Practicexxxxxx', 'The Teamxxxxxxxxxxxx']<br />
<br />
nodes:{"The French Froggies Project!":{color:"#51C215", shape:"img", alpha:1, link:'https://2012.igem.org/Team:Evry/Project', url:'https://static.igem.org/mediawiki/2012/c/cf/French.png'}, //Attribut url pour l'image ajouté /*Thibault*/<br />
<br />
"Xenopus as a chassis":{color:"#63a358", shape:"img", alpha:1, link:'https://2012.igem.org/Team:Evry/FrenchFrog', url:'https://static.igem.org/mediawiki/2012/b/bd/Xenope.png'}, //Attribut url pour l'image ajouté /*Thibault*/<br />
"Xenopus plasmids":{color:"#63a358", alpha:0, link:'https://2012.igem.org/Team:Evry/FrenchFrog#plasmid'},<br />
"Injection in Xenopus":{color:"#63a358", alpha:0, link:'https://2012.igem.org/Team:Evry/InjectionTuto'},<br />
"Development stages":{color:"#63a358", alpha:0, link:'https://2012.igem.org/Team:Evry/Stages'},<br />
"Characterization":{color:"#63a358", alpha:0, link:'https://2012.igem.org/Team:Evry/Tadpole_injection1'},<br />
"Collaboration with Slovenia":{color:"#63a358", alpha:0, link:'https://2012.igem.org/Team:Evry/TeamSlovenia_collaboration'},<br />
<br />
"Hormonal Communicati":{color:"#852c2b", shape:"img", alpha:1, link:'https://2012.igem.org/Team:Evry/AIDSystem', url:'https://static.igem.org/mediawiki/2012/e/e2/Hormone.png'}, //Attribut url pour l'image ajouté /*Thibault*/<br />
"Auxin emitter":{color:"#852c2b", alpha:0, link:'https://2012.igem.org/Team:Evry/AIDSystem#auxin'},<br />
"Auxin receiver":{color:"#852c2b", alpha:0, link:'https://2012.igem.org/Team:Evry/AIDSystem#AID'},<br />
"Auxin toxicity":{color:"#852c2b", alpha:0, link:'https://2012.igem.org/Team:Evry/AuxinTOX'},<br />
"Auxin uptake":{color:"#852c2b", alpha:0, link:'https://2012.igem.org/Team:Evry/auxin_uptake'},<br />
"Degron system caracterization":{color:"#852c2b", alpha:0, link:'https://2012.igem.org/Team:Evry/TirISystemCaracterization'},<br />
<br />
"Modelingxxxxxxxxxxxx":{color:"#1A5291", shape:"img", alpha:1, link:'https://2012.igem.org/Team:Evry/Modeling', url:'https://static.igem.org/mediawiki/2012/5/5b/ModelingDHIUHEIUHD.png'}, //Attribut url pour l'image ajouté /*Thibault*/<br />
"General Model":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/ODE_model'},<br />
"ODEs derivations":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/auxin_pde'},<br />
"Diffusion":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/Auxin_diffusion'},<br />
"Auxin production":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/auxin_production'},<br />
"Auxin reception":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/auxin_detection'},<br />
"Plasmid diffusion":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/plasmid_splitting'},<br />
"Model integration":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/model_integration'},<br />
"Parameters measurements":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/Experimentals_Parameters'},<br />
<br />
"GoldenBricksxxxxxxxx":{color:"#1A5291", shape:"img", alpha:1, link:'https://2012.igem.org/Team:Evry/GB', url:'https://static.igem.org/mediawiki/2012/a/a4/GoldeN.png'}, //Attribut url pour l'image ajouté /*Thibault*/<br />
<br />
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})(this.jQuery)</div>Cyrpauthttp://2012.igem.org/Team:Evry/TirISystemCaracterizationTeam:Evry/TirISystemCaracterization2012-10-27T02:29:35Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
</br><br />
<center><h1><b>Characterization of the degron system !</b></h1></center><br />
</br><br />
<br />
<p>In between the two jamborees, we finished the construction of our TirI degron system and had the time to test it once in Xenupus. Unfortunately the results were negatives. They are presented in this page.</p><br />
<br />
<h2>Preparation of the RNAs</h2><br />
<br />
<p>Starting from the gene, cloned into pSC1C3 for TirI or pSC2+ for GFP-AID, we amplified them with a sp6 promoter on the front of the gene and PCR purified. The mRNAs were then prepared from the PCR product using the Sp6 phage RNA polymerase. The RNAs were then precipitated and poly-adenilated with a RNA poly-adelylase. The mRNA were then treated with a mix of capping proteins before being injected.</p><br />
<br />
<p>The RNAs of TirI and GPD-AID were then pooled in equimolar ratio before being injected for the GFP-AID TirI.</p><br />
<br />
<h2>GFP-AID - Pep2A - TirI in pCS2+</h2><br />
<br />
<p>The two genes GFP-AID and TirI were fused with a Pep2A peptide, to be transcribed as a single protein. The Pep2A sequence comes from the Thosea<br />
asigna virus 2A, creating a peptide bridge in between two proteins. When the ribosome transcribe this sequence protein, one of the amino-acids bound formation is not catalyzed, giving birth to two distinct protein. This is a common method to design polysistronic mRNA in eukaryotes. It is also especially relevant system to use here, because it gives a one to one protein ratio, that we wanted to achieve to make sure the GFP will be properly degraded in the presence of auxin.</p><br />
<br />
<h2>Protocol</h2><br />
<br />
<p>The embryos coming from the in-vitro fertilization were micro-injected from 1 cell to 10 cell stage, and left at 22°C overnight. On the morning, we sorted them and prepared different groups. After the first picture, we placed the tadpoles in a solution containing 5 mM of auxin IAA. Four hours later, we observed the tadpole again and tried to observe a decrease of the fluorescence signal. Unfortunately we didn't observe any noticeable change, and most of our tadpoles died during the 4 hours.</p><br />
<br />
<h2>Results</h2><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012/9/95/TheDegronExperiment.png" width="600px"/><br />
</center><br />
<br />
<h2>In order to go further</h2><br />
<br />
<p>Further controls that needs to be done. The first one is to check the correct expression of TirI doing immuno-fluorescence on the myc tag that is fused to the TirI. We should also try with a longer incubation time, but since all our tadpoles died during the process, this was not doable for this experiment. We are going to re-do this experiment after the jamborees with this additional controls for publication if it works.</p><br />
<br />
<br />
<script type="text/javascript">writeFooter()</script> <br />
</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/TirISystemCaracterizationTeam:Evry/TirISystemCaracterization2012-10-27T02:28:29Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
</br><br />
<center><h1><b>Characterization of the degron system !</b></h1></center><br />
</br><br />
<br />
<p>In between the two jamborees, we finished the construction of our TirI degron system and had the time to test it once in Xenupus. Unfortunately the results were negatives. They are presented in this page.</p><br />
<br />
<h2>Preparation of the RNAs</h2><br />
<br />
<p>Starting from the gene, cloned into pSC1C3 for TirI or pSC2+ for GFP-AID, we amplified them with a sp6 promoter on the front of the gene and PCR purified. The mRNAs were then prepared from the PCR product using the Sp6 phage RNA polymerase. The RNAs were then precipitated and poly-adenilated with a RNA poly-adelylase. The mRNA were then treated with a mix of capping proteins before being injected.</p><br />
<br />
<p>The RNAs of TirI and GPD-AID were then pooled in equimolar ratio before being injected for the GFP-AID TirI.</p><br />
<br />
<h2>GFP-AID - Pep2A - TirI in pCS2+</h2><br />
<br />
<p>The two genes GFP-AID and TirI were fused with a Pep2A peptide, to be transcribed as a single protein. The Pep2A sequence comes from the Thosea<br />
asigna virus 2A, creating a peptide bridge in between two proteins. When the ribosome transcribe this sequence protein, one of the amino-acids bound formation is not catalyzed, giving birth to two distinct protein. This is a common method to design polysistronic mRNA in eukaryotes. It is also especially relevant system to use here, because it gives a one to one protein ratio, that we wanted to achieve to make sure the GFP will be properly degraded in the presence of auxin.</p><br />
<br />
<h2>Protocol</h2><br />
<br />
<p>The embryos coming from the in-vitro fertilization were micro-injected from 1 cell to 10 cell stage, and left at 22°C overnight. On the morning, we sorted them and prepared different groups. After the first picture, we placed the tadpoles in a solution containing 5 mM of auxin IAA. Four hours later, we observed the tadpole again and tried to observe a decrease of the fluorescence signal. Unfortunately we didn't observe any noticeable change, and most of our tadpoles died during the 4 hours.</p><br />
<br />
<h2>Results</h2><br />
<br />
<center><br />
<img src="https://static.igem.org/mediawiki/2012/9/95/TheDegronExperiment.png" /><br />
</center><br />
<br />
<h2>In order to go further</h2><br />
<br />
<p>Further controls that needs to be done. The first one is to check the correct expression of TirI doing immuno-fluorescence on the myc tag that is fused to the TirI. We should also try with a longer incubation time, but since all our tadpoles died during the process, this was not doable for this experiment. We are going to re-do this experiment after the jamborees with this additional controls for publication if it works.</p><br />
<br />
<br />
<script type="text/javascript">writeFooter()</script> <br />
</html></div>Cyrpauthttp://2012.igem.org/File:TheDegronExperiment.pngFile:TheDegronExperiment.png2012-10-27T02:28:00Z<p>Cyrpaut: </p>
<hr />
<div></div>Cyrpauthttp://2012.igem.org/Team:Evry/TirISystemCaracterizationTeam:Evry/TirISystemCaracterization2012-10-27T01:32:37Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
</br><br />
<center><h1><b>Characterization of the degron system !</b></h1></center><br />
</br><br />
<br />
<p>In between the two jamborees, we finished the construction of our TirI degron system and had the time to test it once in Xenupus. Unfortunately the results were negatives. They are presented in this page.</p><br />
<br />
<h2>Preparation of the RNAs</h2><br />
<br />
<p>Starting from the gene, cloned into pSC1C3 for TirI or pSC2+ for GFP-AID, we amplified them with a sp6 promoter on the front of the gene and PCR purified. The mRNAs were then prepared from the PCR product using the Sp6 phage RNA polymerase. The RNAs were then precipitated and poly-adenilated with a RNA poly-adelylase. The mRNA were then treated with a mix of capping proteins before being injected.</p><br />
<br />
<p>The RNAs of TirI and GPD-AID were then pooled in equimolar ratio before being injected for the GFP-AID TirI.</p><br />
<br />
<h2>GFP-AID - Pep2A - TirI in pCS2+</h2><br />
<br />
<p>The two genes GFP-AID and TirI were fused with a Pep2A peptide, to be transcribed as a single protein. The Pep2A sequence comes from the Thosea<br />
asigna virus 2A, creating a peptide bridge in between two proteins. When the ribosome transcribe this sequence protein, one of the amino-acids bound formation is not catalyzed, giving birth to two distinct protein. This is a common method to design polysistronic mRNA in eukaryotes. It is also especially relevant system to use here, because it gives a one to one protein ratio, that we wanted to achieve to make sure the GFP will be properly degraded in the presence of auxin.</p><br />
<br />
<h2>Protocol</h2><br />
<br />
<p>The embryos coming from the in-vitro fertilization were micro-injected from 1 cell to 10 cell stage, and left at 22°C overnight. On the morning, we sorted them and prepared different groups. After the first picture, we placed the tadpoles in a solution containing 5 mM of auxin IAA. Four hours later, we observed the tadpole again and tried to observe a decrease of the fluorescence signal. Unfortunately we didn't observe any noticeable change, and most of our tadpoles died during the 4 hours.</p><br />
<br />
<h2>Results</h2><br />
<br />
<center><br />
<img src="" /><br />
</center><br />
<br />
<h2>In order to go further</h2><br />
<br />
<p>Further controls that needs to be done. The first one is to check the correct expression of TirI doing immuno-fluorescence on the myc tag that is fused to the TirI. We should also try with a longer incubation time, but since all our tadpoles died during the process, this was not doable for this experiment. We are going to re-do this experiment after the jamborees with this additional controls for publication if it works.</p><br />
<br />
<br />
<script type="text/javascript">writeFooter()</script> <br />
</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/TirISystemCaracterizationTeam:Evry/TirISystemCaracterization2012-10-27T01:19:59Z<p>Cyrpaut: Created page with "{{:Team:Evry/template_v1}} <html> </br> <center><h1><b>Characterization of the degron system !</b></h1></center> </br> <p>In between the two jamborees, we finished the construct..."</p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<html><br />
</br><br />
<center><h1><b>Characterization of the degron system !</b></h1></center><br />
</br><br />
<br />
<p>In between the two jamborees, we finished the construction of our TirI degron system and had the time to test it once in Xenupus. Unfortunately the results were negatives. They are presented in this page.</p><br />
<br />
<h2>Preparation of the RNAs</h2><br />
<br />
<p>Starting from the gene, cloned into pSC1C3 for TirI or pSC2+ for GFP-AID, we amplified them with a sp6 promoter on the front of the gene and PCR purified. The mRNAs were then prepared from the PCR product using the Sp6 phage RNA polymerase. The RNAs were then precipitated and poly-adenilated with a RNA poly-adelylase. The mRNA were then treated with a mix of capping proteins before being injected.</p><br />
<br />
<p>The RNAs of TirI and GPD-AID were then pooled in equimolar ratio before being injected for the GFP-AID TirI.</p><br />
<br />
<h2>GFP-AID - Pep2A - TirI in pCS2+</h2><br />
<br />
<p>The two genes GFP-AID and TirI were fused with a Pep2A peptide, to be transcribed as a single protein. The Pep2A sequence comes from the Thosea<br />
asigna virus 2A, creating a peptide bridge in between two proteins. When the ribosome transcribe this sequence protein, one of the amino-acids bound formation is not catalyzed, giving birth to two distinct protein. This is a common method to design polysistronic mRNA in eukaryotes. It is also especially relevant system to use here, because it gives a one to one protein ratio, that we wanted to achieve to make sure the GFP will be properly degraded in the presence of auxin.</p><br />
<br />
<h2>Protocol</h2><br />
<br />
<p>The <br />
<br />
<br />
<script type="text/javascript">writeFooter()</script> <br />
</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/template.jsTeam:Evry/template.js2012-10-27T00:38:51Z<p>Cyrpaut: </p>
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</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/template.jsTeam:Evry/template.js2012-10-27T00:33:37Z<p>Cyrpaut: </p>
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</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/GBTeam:Evry/GB2012-10-27T00:29:22Z<p>Cyrpaut: </p>
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<h1 align=center><b>The GoldenBricks:</b> A fast cloning technique <br> for the PartsRegistry</h1><br />
<br />
<center><img src="https://static.igem.org/mediawiki/2012/9/9e/S%C3%A9lection_163.png" width=250px></center><br />
<br />
<h1>Introduction</h1><br />
<br />
<h2>Context</h2><br />
<br />
<br />
<p>Cloning is probably the most tedious and less rewarding task when engineering living entities through synthetic biology. With the development of the restriction enzymes and efficient DNA ligases, assembling small and large DNA pieces has become a common practice in biology, but as everyone is using their own sets of enzyme and assembly method, making large DNA constructs is still a challenge today. The first indisputable advance in the domain came with the invention of the first standardized DNA cloning method: the BioBricks [1]. The standard BioBrick format (BBF RFC 10) make possible to clone together all kind of genetic parts (called "bricks") using only four different enzymes: EcoRI, XbaI, SpeI and PstI. But the real power of BioBrick standardization lays in the possibility to build database made of these DNA pieces that anyone can be assembled through a standard method. The PartsRegistry today contains almost over 3,000 basic characterized biological parts [2] and is a sources of inspiration for thousands of biologists around the world.<p><br />
<br />
<p>If biobricks have opened new perspectives for engineering organisms and have proven their efficiency over a decade, their use is limited by the fact that the fragments can only be assembled one by one. On the top of this, the process is very difficult to automatize, because of the number of DNA purification step required. IGEMers today spend most of their time assembling genetic parts together, leaving little time for characterizing them and testing their systems.</p><br />
<br />
<p>Several cloning techniques capable of assembling multiple fragments at the time have been invented since the rise of the Biobrick format. The most popular one is probably the one known as the Gibson method [3] that can be used to assemble up to four fragments at the time on a regular basis. The Golden Gate technique [4] (>20 fragments at the time) and its new evolution, the MoClo [5] (47 fragments in two times reported) are leading the way of another kind of cloning technique based on type II restriction enzymes. More efficient than the Gibson cloning, the Golden Gate also have the huge avantage that it can assemble more parts at the time, no matter their size. Moreover, Golden Gate do not imply any DNA modifications, which noticeably reduce the probability of having a mutation at the ligation scar.</p><br />
<br />
<p>If these new techniques dramatically speed-up the assembly of DNA pieces, to our knowledge, no reported work has been carried on standardizing these methodologies. So far, biologists using Golden Gate engineer new overhangs and create a new library each time they are making a different construct.</p><br />
<br />
<br />
<h2>From Golden Gate and BioBricks to GoldenBricks</h2><br />
<br />
<p>The BioBricks are a collection of parts that can be assembled one by one in a generic way. Golden Gate is a technique that enable the assembly of several tens of parts simultaneously. Taking inpiration from both technique, the 2012 Evry iGEM Team invented and developped a new methodology called GoldenBricks, merging the power of the two techniques while keeping the compatibility toward the old RFC 10 BioBrick format and preserving the possiblilty to make standard GoldenGate assembly simultaneously with GoldenBrick assembly, using the same vectors.<p><br />
<br />
<p>GoldenBricks technique is a one-shot cassette construction using DNA parts coming either from a plasmid distribution or from PCR product, engaging 5, 7 and possibly more different parts. Moreover, GoldenBricks works for both eukaryotes and procaryotes DNA construction with the very same protocol. If a non classical assembly is required (as for testing the strength of a terminator) a new "split construction" method based on standard plasmids make possible to assemble parts in a non classical way.</p><br />
<br />
<h2>Perspectives</h2><br />
<br />
<p>Such technique offers great perspectives for the future of iGEM and of the PartsRegistry in general. First, it would make possible the fast and simple assembly of complete cassettes using less expensive equipment, less toxic chemical and less sequencing runs than the BioBrick assembly. Second, it would make fast and efficient cloning accessible for both researchers as well as for less experimented users, such as high school iGEMers or biohackers thanks to the simplicity of its protocol. Third, the creation of large expression cassettes using this method is cheaper and faster than synthesizing the construct at the present day, which would guaranty the interest for a DNA database compared to <i>de novo</i> synthesis for the years to come. And last, this method is a lot more easy to automatize unlike the RFC 10 biobrick format.</p><br />
<br />
<h1>Theory</h1><br />
<br />
<a name="requirement" ></a><h2>Requirements for the development of the new standard</h2><br />
<br />
<p>In the continuation of what we wrote previously and in order to stay in the progression of the methodology we have tried in this work to stay as close as possible to the biobrick format, to keep GoldenBrick parts compatible with the RFC 10 standard assembly, while opening new perspectives to increase the speed and the easiness of cloning. These constraints imposed several requirements for this new standard.</p><br />
<br />
<p>First, the new assembly method should:</p><br />
<ol><br />
<li>Keep the compatibility with the current BBF RFC 10 standard</li><br />
<li>Be compatible with a database approach</li><br />
<li>Minimize the number of different prefixes and suffixes</li><br />
<li>Use the standard registry plasmids and negative cloning control</li><br />
</ol><br />
<br />
<p>To improve the cloning speed, we would also like to:</p><br />
<ol start=5><br />
<li>Make one step Golden Gate cassette cloning possible</li><br />
<li>Allow to check the construct with a single enzyme digestion</li><br />
<li>Improve the compatibility with Gibson by removing the NotI site</li><br />
<li>Provide a solution for non standard assemblies</li><br />
</ol><br />
<br />
<p>As we will demonstrate, the GoldenBrick format we have established meet all these requirements. To understand on what basis we have created it, we are now going to explain in this section the principles that led the design of this format before we analyze the sequences of the GoldenBrick prefixes and suffixes and finally discuss what can be done with this technique.<br />
<br />
<h2>A brief introduction on type II restriction enzymes</h2><br />
<br />
<p>Golden Gate cloning, (also known as type II restriction enzyme cloning) rely on a specific familly of enzyme (known as the type IIS enzymes) very different from the ones we are used to. The characteristic of type IIS enzymes from the cloning point of view is that these enzymes cut the DNA outside their recognition locus, no matter the sequence of the place where they cut. A very small subtype of them can be used for cloning purpose, since they generate overhangs on one side of the recognition site only. The ones used for Golden Gate and MoClo cloning are BsaI and BbsI.</p><br />
<br />
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:202px;"><a href="https://static.igem.org/mediawiki/2012/0/0b/S%C3%A9lection_164.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2012/0/0b/S%C3%A9lection_164.png" width="200" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="https://static.igem.org/mediawiki/2012/0/0b/S%C3%A9lection_164.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 2: The recognition pattern of BsaI and cutting site.</div></div></div></div><br />
<br />
<br /><br />
<br />
<p>The fact that these enzymes cut outside its recognition site gives them two significant advantages:</p><br />
<ul><br />
<li>You can generate as many different overhang you want with a single enzyme within the same tube</li><br />
<li>You can engineer your fragment so that it cannot be cutted again after it has ligated.</li><br />
</ul><br />
<br />
<p>Thanks to this last point, it is possible to cut and ligate the DNA in the mean time within the same tube (BsaI works in the T4 ligase buffer), and do not require any DNA purification. For this reason, the ligation is achieved with a better yield and with less mutation than with traditional type II enzymes cloning methods.</p><br />
<br />
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:502px;"><a href="https://static.igem.org/mediawiki/2012/9/90/S%C3%A9lection_165.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2012/9/90/S%C3%A9lection_165.png" width="500" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="https://static.igem.org/mediawiki/2012/9/90/S%C3%A9lection_165.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Figure 3: Principle of the Golden Gate cloning method.</div></div></div></div><br />
<br />
<p>Since the small BsaI fragment cut can re-ligate with the mother strand, the assembly is a lot more efficient if the tube is alternatively placed at 37°C, when the BsaI cuts and then placed at 16°C for a ligation time, using a thermocycler. This technique drive the equilibrium towards the product formation.</p><br />
<br />
<h2>Analysis of a classical synthetic biology construction</h2><br />
<br />
<p>A traditional construct in synthetic biology is made of the repetition of the following elements:</p> <br />
<br />
<br />
<br />
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:402px;"><a href="https://static.igem.org/mediawiki/2012/6/6e/Constructs.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2012/6/6e/Constructs.png" width="400" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="https://static.igem.org/mediawiki/2012/6/6e/Constructs.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 4: Schematic of the different elements assembly in a standard synthetic biology device.</div></div></div></div><br />
<br />
<p>At the moment, GoldenBrick only allows the assembly of a single cassette at the time. In order to create the full system, the assembly of the different cassettes together have to be conducted afterwards with the biobrick assembly, Gibson assembly - that is very efficient to assemble long fragments - or ligation independant cloning [6]. Focusing closer on a single cassette, we can notice that we have four different kinds of junctions:</p><br />
<br />
<br /><br />
<br />
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:502px;"><a href="https://static.igem.org/mediawiki/2012/2/26/Constructs_anotated.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2012/2/26/Constructs_anotated.png" width="500" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="https://static.igem.org/mediawiki/2012/2/26/Constructs_anotated.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 5: In a single cassette, we can notice four different kinds of junctions. If we want the repetition of the {RBS-CDS} unit to be possible, the prefix of the RBS have to be compatible with the suffix of the gene.</div></div></div></div><br />
<br />
<p>As we can notice on this picture, there is four different kinds of junctions in an expression cassette. In Golden Gate, the junctions are DNA overhangs that can be freely engineered thanks to the capacity of Type IIS enzymes to cut outside their recognition site, no matter what sequence is there. Therefore, we should engineer four different overhangs. The first overhang is dedicated to the ligation of the plasmid suffix with the promoter prefix (OH1). Similarly, the 5th one is dedicated to ligate the terminator suffix with the backbone prefix (OH5). In a bacterial device, the scar between the RBS and the gene (OH3) has to be precisely controlled because the distance between the two elements is critical for correct protein expression. This scar will remain the same than in the biobrick assembly, but it will be generated by a type IIS enzyme as for the other overhangs. Finally, the overhang that link the RBS prefix to the promoter suffix (OH2) and the protein suffix to the terminator prefix (OH4) have to be compatible to make the repetition of several {RBS-Protein} units in a single cassette (OH2=OH4) possible. We will see later how we can achieve control of this repetition.</p><br />
<br />
<p>To conclude, we have five different kinds of parts, which implies to create five different prefixes and five different suffixes with four different overhangs, which will help to assemble the five different DNA parts in the correct order.</p><br />
<br />
<p>In addition, we would like to generate a scar that can be digested by another enzyme, in order to control whether all the elements we intended to insert are present in the cassette or not, before sequencing it. As we will see later this element is critical for the selection of the clone that have inserted all the genes we want and avoid sequencing incorrect constructions. In order to generate such a scar, the overhangs 1, 2, 4 and 5 has been engineered in the middle of a BbsI site. When two pieces ligates, they recreate a BbsI restriction site, and the construct can be checked with a simple BbsI digestion.</p><br />
<br />
<p>One last condition to fulfill to get a RFC 10 compatible brick after GoldenBrick cloning is to leave no illegal restriction site inside the brick after the assembly of the cassette. However, we should keep the as similar as possible the BioBrick extensions around each elements leaving the four usual enzyme restriction sites, so that the brick can be still assembled using RFC 10 BioBrick method. The only drawback is that if several GoldenBrick parts are assembled with RFC 10 standard, they cannot be assembled with other GoldenBrick parts afterwards, because the BioBrick assembly will leave illegal BsaI site inside the casette.</p><br />
<br />
<h2>The proposed new set of Golden Bricks extension</h2><br />
<br />
<p>Having all these design principles in mind and meeting the requirements we have proposed, we designed the new GoldenBrick prefix and suffix with the following sequences:</p><br />
<br />
<br />
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:635px;"><a href="/File:GoldenBricks.png" class="image"><img alt="" src="/wiki/images/e/ed/GoldenBricks.png" width="633" height="286" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="/File:GoldenBricks.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Figure 6: Sequence and design of the different prefixes and suffixes used in the GoldenGate cloning method. The presence of the EcoRI, XbaI, SpeI and PstI keep the compatibility with Biobrick cloning while the BsaI site enable single shot GoldenGate assembly. The BbsI sites serve as a single digestion control.</div></div></div></div><br />
<br />
<p>This design fulfills all the requirements enumerated before and ensure that the different elements will be assembled in the correct order. We also generated a scar that can be digested afterwards with the BbsI enzyme, so that the correct insertion of the different elements can be checked afterwards by doing a single digestion. This also enable the polymerization of the {RBS-CDS} units, as we will discuss in the next paragraph.</p><br />
<br />
<h2>Control over the polymerization</h2><br />
<br />
<p>The only difference with a standard GoldenBrick protocol is the capacity for the RBS-protein cassette to polymerize. This polymerization capacity makes the power of the technique because it enables to make polycistronic mRNAs no matter what gene is inserted. However, a control mechanism is required in order to create polycistrons or when we want a single gene to be inserted in the cassette.</p><br />
<br />
<p>In order to achieve such a control, several parameters in the protocol can be ajusted to move the equilibrium from monocistrons to polycistrons. The first and the most obvious one is the stochiometry of the different elements. If the promoters and terminators are in large excess compared to the RBSs and the gene, the balance will be in favour of monocistrons. On the contrary, if the amount or RBSs and coding sequence in dominant, the cassette will tend to contain two or more genes inserted inside.</p><br />
<br />
<p>On the other hand, the number of cycle and the ligation time will also influence the polymerization. Polycistrons are not likely to appear when the number of cycle is small and the ligation time short, but they will become more and more important as we increase these parameters.</p><br />
<br />
<p>Most of the work carried on by our team is now focused on optimizing the protocol. We will release soon a set of standard protocols for mono and polycistrons optimized to reduce the heterogeneity of the polymerization length.</p><br />
<br />
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:502px;"><a href="/File:GoldenBrick-polymerization.png" class="image"><img alt="" src="/wiki/images/1/10/GoldenBrick-polymerization.png" width="500" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="/File:GoldenBrick-polymerization.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 8: a. The vector, promoter, and terminator are in excess: the assembly produces mostly monocistrons. b. The protein sequences and RBS are in excess: the assembly produces mostly polycistrons.</div></div></div></div><br />
<br />
<br />
<h2>Assembly procedure</h2><br />
<br />
<div class="thumb tright"><div class="thumbinner" style="width:302px;"><a href="/File:GOldenbrick_global_thumb.png" class="image"><img alt="" src="/wiki/images/thumb/f/f3/GOldenbrick_global_thumb.png/300px-GOldenbrick_global_thumb.png" width="300" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="/File:GOldenbrick_global_thumb.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 9: Summary of the GoldenBrick procedure</div></div></div><br />
<br />
<br />
<p>Assembling parts using the GoldenBrick method is a lot easier than for BioBricks and also five to ten times faster. We are now going to explain the procedure from the theoretical point of view. For the complete protocol, you should refer to the material and methods section.</p><br />
<br />
<p>Because it requires a very small amount of DNA, the plasmid source can be taken directely from a DNA distribution suck as the PartsRegistry distribution, without requiring the need of a DNA preparation before. Once the plasmids are resuspended, the experimentalist mix them together in a mix containing the BsaI enzyme and T4 ligase and put them in the thermocycler for 5 hours. At the end, the product can be directly transformed and plated. A first round of screening on the good polymerization length can be made using colony PCR with the standard VF2 and VR primers. If required, a second step of screening can be made after miniprepping using BbsI. Given the very small rate of mutation, only two clones with the good digestion or colony PCR signature can be sent for sequencing; they will contain the correct construct with a very high probability.</p><br />
<br />
<p>This technique however requires to screen an important number of clones because we don't have a total control over what is assembled inside the cassette, because of the freedom we left for the RBS-Protein entities to polymerize. We will show in the next paragraph how we can gain a relative control over it. To reduce the costs of screening, we encourage the use of colony PCR over mini-preping and digestion when possible.</p><br />
<br />
<br />
<h1>Other advantages of GoldenBricks over any other standard cloning techniques</h1><br />
<br />
<h2>Non standard assembly and "split-construct" vectors</h2><br />
<br />
<p>If you are willing to create a non standard assembly or to control very precisely RBS-protein pairing over a polycistrons, you might like to create the construct in two times and assemble them together in a second step. This is why we have created the "split-construct" vectors. The split-contruct vectors are similar to the goldenbrick vectors except that they contain the OH2-OH5 or the OH1-OH4 overhangs instead of the OH1-OH5 overhangs as the GoldenBrick plasmid.</p><br />
<br />
<p>These vectors enable the assembly of shorter casette containing only a promoter, and a serie of RBS-protein for the "split-construct" 1 vector, or RBS-protein terminator for the "split-construct" 2 vector. This way, if a non standard biobrick assembly has to be carried out in between the two pieces or if a long polycistron has to be created, they can be assembled using the standard EcoRI, XbaI, SpeI, PstI cloning or Gibson assembly, but several cloning step will be saved by using GoldenBricks compared to the standard assembly of the full cassette.</p><br />
<br />
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:802px;"><a href="https://static.igem.org/mediawiki/2012/1/19/Split-constructs.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2012/1/19/Split-constructs.png" width="800" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="https://static.igem.org/mediawiki/2012/1/19/Split-constructs.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 10: Description of the construction of a non classical cassette for creating a device that test the strength of a terminator using split-vector GoldenBricked mixed with classical or Gibson assembly. The GFP and RFP ratios are measured to evaluate the strength of a terminator the classical way. Two cloning steps are avoided using this procedure.</div></div></div></div><br />
<br />
<br />
<h2>GoldenBrick make standard part DNA shuffling possible</h2><br />
<br />
<p>Aside from the dramatic shortening of the cloning time, the decrease of the costs and the easiness of GoldenBricks over Biobricks, one of the most noticeable improvement is undoubtedly the possibility to make DNA shuffling using a standard format. Let us take the example of a complex system such as a toggle-switch, in which you would like to optimize the expression of the protein repression of its two components to place the system in the working area of the phase domain.</p><br />
<br />
<p>Using the Biobrick format, you will probably have to make tens of different constructs with different RBS to find a condition in which the toggle-switch will work. Using GoldenBricks, you can make all of them in a single tube, in a single step. Moreover, you may eventually find a way to screen them directly after the cloning avoiding the need of purifying and sequence all the clones that couldn't work.</p><br />
<br />
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:802px;"><a href="https://static.igem.org/mediawiki/2012/3/31/DNA-shuffeling.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2012/3/31/DNA-shuffeling.png" width="800" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="https://static.igem.org/mediawiki/2012/3/31/DNA-shuffeling.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 11: Description of a DNA shuffling experiment in order to optimize the expression of a single gene. 4 Promoters and RBSs are mixed together in a single tube and give rise to 16 construct in a single tube in a single step. The clone expression the enzyme at the correct level can be screened directly on the transformation plate.</div></div></div></div><br />
<br />
<p>Many other use of DNA shuffling can be found in the literature especially for gene expression optimization and screening for working enzymes. We expect that DNA shuffling over standard parts is going to find many applications in the future of iGEM projects.</p><br />
<br />
<h2>GoldenBrick is still compatible with Biobrick format</h2><br />
<br />
<p>If the situation requires the part to be assembled with the standard RFC 10 format, the new GoldenBrick parts can be assembled the very same way than BioBricks. The only limitation is that BioBrick assembly of GoldenBrick parts leave some scars containing the BsaI restriction site inside, and the produced parts cannot be reassembled with other parts using Golden Gate. No simple way of fixing this issue has been found.</p><br />
<br />
<h2>GoldenBricked plasmids are compatible with standard Golden Gate assembly</h2><br />
<br />
<p>The GoldenBricked plasmids can be used to assemble contructions using classical Golden Gate. This option has been tested in our team and it works fine. See the result section for more results on the use of GoldenBrick vectors with the construction of the IaaH-Pep2A-IaaM fusion protein.</p> <br />
<br />
<h2>GoldenBrick also assemble PCR products and shorten the assembly time</h2><br />
<br />
<p>Someone that want to create a construct not having the time or the need of cloning his gene into a vector first can still assemble them using GoldenBrick. Although this practice wouldn't be encouraged for standards parts, it can become really useful when trying libraries of different genes in order to find a working one. GoldenBricked parts in plasmid and PCR GoldenBricked parts can be mixed together and assembled the very same way. The user will just have to take care to submit his working parts afterwards. We recommend to amplify new parts flanked by its GoldenBricks prefix and suffix, mix them with the GoldenGate assembly mix in one hand, and digest them in EcoRI and PstI on the other hand and ligate them in pSB1C3 for submitting the GoldenBricked part to the registry.<br />
<br />
<h2>Classical GoldenGate can be carried out in the mean time than GoldenBrick assembly to create fusion proteins within the construct</h2><br />
<br />
<p>GoldenGate in general and GoldenBricks in particular are very efficient to assemble fusion proteins. This is because one can design every overhang it wants and therefore fuse protein domains without leaving any scar. New fusion proteins can be created in the meantime they are assembled in the cassette, using an aditional scar designed by the experimentalist. We also recommand to submit the new fusion protein part separately to contribute to the registry.</p><br />
<br />
<h2>Finally, GoldenBricks works for eukaryotes chassis as well as with prokaryotes</h2><br />
<br />
<p>As we have seen all along this article, this design of GoldenBricks have been designed for procaryotes, but it can work very well for eukaryotes. In eukaryotes, promoters can include or not a 5'UTR in their sequence to enhance the protein expression. In the case there is no 5'UTR, we can use the same prefix and suffix than the prokaryote promoters and include the 5'UTR with the prokaryotes prefix and suffix of a prokaryotic RBS.</p><br />
<br />
<p>In order to make polycistrons, we can use an Intermediate Ribosome Entry Site (IRES), cloned between an prokaryote RBS prefix and suffix.</p><br />
<br />
<p>Finally, the polyA and the polyA capping cassette with a polymerase terminator can be flanked by the prokaryotes terminators sites. In the case one don't want to include a polyA capping, he can use the "split construct" 1 or in the case there is not terminator, the one on the plasmid will take care of stopping the RNA polymerase extension.</p><br />
<br />
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:802px;"><a href="https://static.igem.org/mediawiki/2012/0/0a/GoldenBricks-eukaryote.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2012/0/0a/GoldenBricks-eukaryote.png" width="800" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="https://static.igem.org/mediawiki/2012/0/0a/GoldenBricks-eukaryote.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 12: GoldenBricks prefixes and suffixes for eukaryotic constructions.</div></div></div></div><br />
<br />
<h1>Results</h1><br />
<br />
<h2>Construction of the library</h2><br />
<br />
<p>In order to test the GoldenBrick technique efficiently, and for easiness of screening and statistics, we have developed a set of GoldenBricked parts (see Material & Methods) with fluorescent proteins markers. We have created and submitted the following parts:</p><br />
<br />
<ul><br />
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812050">K812050:</a> A GoldenBricked version of pSB1C3 with J04450 as negative cloning control (status=sent)</li><br />
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812051">K812051:</a> A GoldenBricked version of pSB1K3 with J04450 as negative cloning control (status=constructed)</li><br />
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812053">K812053:</a> A GoldenBricked version of the strong RBS B0034 (status=sent)</li><br />
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812054">K812054:</a> A GoldenBricked version of the RFP E1010 (status=sent)</li><br />
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812055">K812055:</a> A GoldenBricked version of the terminator B0015 (status=sent)</li><br />
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812056">K812056:</a> A GoldenBricked version of the pLac R0010 promoter (status=sent)</li><br />
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812057">K812057:</a> A GoldenBricked of an sfGFP protein (status=constructed)</li><br />
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812058">K812058:</a> A GoldenBricked of medium strenght RBS J61107 (status=constructed)</li><br />
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812058">K812059:</a> A GoldenBricked of week RBS J61117 (status=constructed)</li><br />
</ul><br />
<br />
<p>All the parts written here have been successfully constructed and sequenced and the ones marked as "sent" has been sent to the PartsRegistry.</p><br />
<br />
<a name="GB" ></a><h2>Creation of a simple cassette</h2><br />
<br />
<p>We have started experimenting on the GoldenBrick assembly method. For the moment, the results are not optimal so we are not publishing them yet. Better results are comming soon. For the moment, we have about 1% of correct clones.</p><br />
<br />
<a name="GG" /></a><h2>Working with regular GoldenGate using GoldenBricks vectors</h2> <br />
<br />
<p>In order to assemble the fusion proteins we need for our project, we carried out two assemblies of fusion proteins using Golden Gate in our Golden Gated vectors. This paragraph reports the construction of the IaaH-Pep2A-IaaM fusion protein in the GoldenBrick plasmid K812050 using standard Golden Gate protocol. This protocol hadn't been optimized at the moment. However, out of 16 cloned on which we did colony PCR, 10 of them had the good size. We sequenced 4 of them and obtained the sequence expected.</p><br />
<br />
<br />
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:702px;"><a href="https://static.igem.org/mediawiki/2012/thumb/4/40/IaaH-Pep2A-IaaM.png/800px-IaaH-Pep2A-IaaM.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2012/thumb/4/40/IaaH-Pep2A-IaaM.png/800px-IaaH-Pep2A-IaaM.png" width="700" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="https://static.igem.org/mediawiki/2012/thumb/4/40/IaaH-Pep2A-IaaM.png/800px-IaaH-Pep2A-IaaM.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 13: Colony PCR of 15 clones from the transformation plate. The ones marked with a star were sequenced and proved to be correct. The one with an exclamation mark was sequenced and had only one of the CDS inserted.</div></div></div></div><br />
<br />
<p>This experiment demonstrates the possibility of doing GoldenGate in the GoldenBricks plasmids K812050 (and similarly in K812051) in a very efficient way.</p><br />
<br />
<br />
<h1>Material and methods</h1><br />
<br />
<h2>Construction of the library</h2><br />
<br />
<p>The GoldenBricked parts were constructed amplifying the corresponding biobricks using primers flanked by the appropriate GoldenBricks extensions, using Phusion (Thermo Scientific) or Q5 (NEB) DNA polymerase in a 30 cycle reaction. The PCR products were PCR purified and then digested using FastDigest® EcoRI and PstI (Thermo Scientific). The standard plasmid pSB1C3 (or pSB1K3) were digested similarely. Both vectors and inserts were PCR purified and then ligated using T4 DNA ligase (Thermo Scientific) before being transformed with home made competent cells (chemical or electroporation). Four white clones were inoculated and sequenced.<p><br />
<br />
<p>The either red or non red colonies were minipreped and then digested with BsaI for screening. All the clones that had the correct digestion pattern were sequenced and gave the correct sequence.</p><br />
<br />
<p>The RBSs were synthetized in the form of two matching oligos. A mixture of 10 µM of the two oligo was incubated at 98°C for 2 min and then cooled down to 4°C with a ramp of -1°C/s. They were then PCR purified, digested and PCR purified again before beeing ligated in pSB1C3.</p><br />
<br />
<h2>Assembly protocol</h2><br />
<br />
<p>The protocol given here hasn't been optimized yet. We are only starting the development of this new technology. It is given here as a matter of indication. Some optimized protocol will be available soon.</p><br />
<br />
<p>About 75 ng of each plasmid (more accurate ratios will be optimized) were mixed together with 2.5 units of BsaI enzyme (NEB) and 15 units of T4 DNA ligase (Thermo Scientific) in a total volume of 15 µL of standard T4 ligation buffer. The tubes were then placed in the thermocycler with the following steps:</p><br />
<br />
<ol><br />
<li>37°C, 2 min (digestion)</li><br />
<li>16°C, 5 min (ligation)</li><br />
<li>Goto 1, 50 times</li><br />
<li><ul><li>Option 1: if the construct does not contain any BsaI site: 50°C 5 min (reduce the background with a final digestion)</li><br />
<li>Option 2: if the construct contains BsaI site: 16°C 2h (reconstitute the BsaI site)</li></ul></li><br />
<li>80°C, 5 min (heat inactivation)</li><br />
</ol><br />
<br />
<p>5 µL of the product was transformed with 50 µL of home made chemically competent cells and recovered for 1 hour. The cells were concentrated and then plated.</p><br />
<br />
<h1>Perspectives for GoldenBricks and the PartsRegistry</h1><br />
<br />
<p>When the complete protocols will be established, our team will submit a BBF RFC request for the registry. If the RFC get accepted and if the tenant of the PartsRegistry thinks this is a valuable format, we can think of migrating the registry to this new format. This can be done in two complementary ways: the first one is to ask every user to submit their new part in the new GoldeBrick format, and the second is to take advantage of the huge community working on the PartsRegistry and especially the iGEM competition. In order to migrate the already existing parts faster, we can add in the requirements for the gold medal to resubmit already exsiting parts in the new format. With an average of 200 teams in the years to come and asking each of them to resubmit two goldenbricked parts, we can cover the 2000 most useful parts within two or three years, because the teams will GoldenBrick the parts they are interested in for their assembly, so they will probably always re-submit more than two.</p><br />
<br />
<p>We believe that implementing such a method in the partsregistry will speed up the assembly process and give a new shine to this database that severely suffers from the gene synthesis and PCR based methods competition. This will leave more time for researchers and iGEMers for characterizing the parts, and the partregistry will also benefits from an increase of parts characterization, which is very beneficial to the community. Overhall, the idea of standardized parts that tends to loose its strength with the time will gain again in interest in the long run.</p> <br />
<br />
<h1>Conclusion</h1><br />
<br />
<p>This year our team created a new cloning format for the PartsRegistry that dramatically speeds up the cloning time and opens new perspective. This includes simple protein fusion methods and DNA shuffling that were not possible using the current BioBrick format. At the moment, we are working very hard to demonstrate the possibilities of the technique and come up with efficient and reliable protocols. If the process proves its interest, we will submit an RFC to the PartsRegistry and propose the GoldenBrick progressively substitute to the current BioBrick format, which will renew the interest for people on standardized biological parts and standardized assembly over gene synthesis.</p><br />
<br />
<br />
<br />
<div id="citation_box"><br />
<p id="references">References:</p><br />
<ol><br />
<li><i>Knight TF</i>, Idempotent Vector Design for Standard Assembly of BioBricks. 2003, Tech rep, MIT Synthetic Biology Working Group Technical Reports.</li><br />
<li>Source: http://partsregistry.org/cgi/partsdb/Statistics.cgi</li><br />
<li><i>Gibson D</i>, One-step enzymatic assembly of DNA molecules up to several hundred kilobases in size. 2009, Nature Publishing Group: doi:10.1038/nprot.2009.77</li><br />
<li><i>Engler C, Gruetzner R, Kandzia R, Marillonnet S</i>, Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes. 2009, PLoS ONE 4(5): e5553. doi:10.1371/journal.pone.0005553</li><br />
<li><i>Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S</i>, A Modular Cloning System for Standardized Assembly of Multigene Constructs, 2011, PLoS ONE 6(2): e16765. doi:10.1371/journal.pone.0016765</li><br />
<li><i>Charalampos A & J. de Joung P</i>, Ligation-independent cloning of PCR products (LIC-PCR), 1990, Nucleic Acids Research 18 (20): 6069-6074. doi: 10.1093/nar/18.20.6069</li><br />
</ol><br />
</div><br />
<br />
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<p>Hi iGEMer! Are you brainstorming on a new project? Do you plan to work collaborate with Xenopus? You are at the good place, this book is dedicated for you.</p><br />
<br />
<p>We are the 2012 Evry iGEM team, and this year, we have made the first iGEM project on Xenopus and created the first biobricks to work with. In this small book, we wanted to share with you our experience with this animal, try to give you guidelines for designing your own project and give you references to the resources you can use.</p><br />
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<p>Hi iGEMer! Are you brainstorming on a new project? Do you plan to work collaborate with Xenopus? You are at the good place, this book is dedicated for you.</p><br />
<br />
<p>We are the 2012 Evry iGEM team, and this year, we have made the first iGEM project on Xenopus and created the first biobricks to work with. In this small book, we wanted to share with you our experience with this animal, try to give you guidelines for designing your own project and give you references to the resources you can use.</p><br />
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<p>Hi iGEMer! Are you brainstorming on a new project? Do you plan to work collaborate with Xenopus? You are at the good place, this book is dedicated for you.</p><br />
<br />
<p>We are the 2012 Evry iGEM team, and this year, we have made the first iGEM project on Xenopus and created the first biobricks to work with. In this small book, we wanted to share with you our experience with this animal, try to give you guidelines for designing your own project and give you references to the resources you can use.</p><br />
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<p>Hi iGEMer! Are you brainstorming on a new project? Do you plan to work collaborate with Xenopus? You are at the good place, this book is dedicated for you.</p><br />
<br />
<p>We are the 2012 Evry iGEM team, and this year, we have made the first iGEM project on Xenopus and created the first biobricks to work with. In this small book, we wanted to share with you our experience with this animal, try to give you guidelines for designing your own project and give you references to the resources you can use.</p><br />
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<br />
<p>We are the 2012 Evry iGEM team, and this year, we have made the first iGEM project on Xenopus and created the first biobricks to work with. In this small book, we wanted to share with you our experience with this animal, try to give you guidelines for designing your own project and give you references to the resources you can use.</p><br />
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<p>Hi iGEMer! Are you brainstorming on a new project? Do you plan to work collaborate with Xenopus? You are at the good place, this book is dedicated for you.</p><br />
<br />
<p>We are the 2012 Evry iGEM team, and this year, we have made the first iGEM project on Xenopus and created the first biobricks to work with. In this small book, we wanted to share with you our experience with this animal, try to give you guidelines for designing your own project and give you references to the resources you can use.</p><br />
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<p>Hi iGEMer! Are you brainstorming on a new project? Do you plan to work collaborate with Xenopus? You are at the good place, this book is dedicated for you.</p><br />
<br />
<p>We are the 2012 Evry iGEM team, and this year, we have made the first iGEM project on Xenopus and created the first biobricks to work with. In this small book, we wanted to share with you our experience with this animal, try to give you guidelines for designing your own project and give you references to the resources you can use.</p><br />
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<p>Hi iGEMer! Are you brainstorming on a new project? Do you plan to work collaborate with Xenopus? You are at the good place, this book is dedicated for you.</p><br />
<br />
<p>We are the 2012 Evry iGEM team, and this year, we have made the first iGEM project on Xenopus and created the first biobricks to work with. In this small book, we wanted to share with you our experience with this animal, try to give you guidelines for designing your own project and give you references to the resources you can use.</p><br />
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<h1>Xenopus for dummies: an iGEMer guidebook</h1><br />
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<p>Hi iGEMer! Are you brainstorming on a new project? Do you plan to work collaborate with Xenopus? You are at the good place, this book is dedicated for you.</p><br />
<br />
<p>We are the 2012 Evry iGEM team, and this year, we have made the first iGEM project on Xenopus and created the first biobricks to work with. In this small book, we wanted to share with you our experience with this animal, try to give you guidelines for designing your own project and give you references to the resources you can use.</p><br />
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<img src="https://static.igem.org/mediawiki/2012/8/86/FrogForDummies_cover.png" alt="XenopusForDumies" width="400px" /><br />
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<div></div>Cyrpauthttp://2012.igem.org/File:FrogForDummies_cover.pngFile:FrogForDummies cover.png2012-10-26T23:56:03Z<p>Cyrpaut: </p>
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<div></div>Cyrpauthttp://2012.igem.org/Team:Evry/ProjectTeam:Evry/Project2012-10-26T23:51:39Z<p>Cyrpaut: </p>
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For our first participation in iGEM, we have decided to introduce a new organism to the competition: <i>Xenopus tropicalis</i>. Its common name is the Western clawed frog, a diploid cousin of the model organism <i>Xenopus laevis</i>. Aside for the soft spot French have for frogs, we also believe <i>Xenopus</i> could be a great multicellular chassis for synthetic biology. We are therefore bringing this organism to iGEM for the first time, along with the tools we need to bring Synthetic biology to the multicellular era.<br />
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<p><br />
The laboratory part of our work can be divided into two categories: the creation of synthetic biology tools for <i>Xenopus</i> and the creation of a synthetic hormonal system. We created a multi-level model of this inter-tissue communication system, concurrently laying the groundwork for modeling of synthetic genetic systems in multicellular organisms. Finally, using vertebrates in synthetic biology poses deep ethical problems, which come alongside those of animal experimentation. iGEM aims to be cool and fun, but can we or should we keep the same attitude when working with vertebrate embryos ? Should we reduce animals to objects or tools by using words such as chassis when working with these multicellular organisms? Our resident philosopher lead our team’s reflection on these issues, proposing a guide for future synthetic biologists who wish to work with <i>Xenopus</i>. <br />
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<h3 style="text-align: center; padding-top:10px;">The French Froggies Project</h3><br><br />
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<a href="#xenopus" style="text-decoration:none;"><li><i>Xenopus</i>: A new multicellular chassis</li></a><br />
<a href="#hormone" style="text-decoration:none;"><li>Intertissue communication: An orthogonal hormonal system </li></a><br />
<a href="#goldenBrick" style="text-decoration:none;"><li>GoldenBrick: A new biobrick cloning format</li></a><br />
<a href="#modeling" style="text-decoration:none;"><li>Modeling a tadpole: A multi-level approach</li></a><br />
<a href="#human" style="text-decoration:none;"><li>Human pratice: What does it mean to be a chassis?</a><br />
<a href="#dummies" style="text-decoration:none;"><li>Xenopus for dummies: an iGEMer guidebook</a><br><br><br />
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<h2 id="xenopus"><i>Xenopus</i>: A new multicellular chassis</h2><br />
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While iGEM has so far been mostly focused on en engineering unicellular organisms and bacteria in particular, we have decided work on the next step for synthetic biology: Multicellular engineering. Using our new biobrick tools, the road towards synthetic circuits in <i>Xenopus</i> is open, multiplying the number of potential applications in terms of synthetic biology.<br />
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<i>Xenopus</i> is a model organism for developmental biology. Rapid development, easy handling and direct injection of plasmids into the fertilized egg allow testing of constructs in less than two weeks. We provide frog compatible plasmids for using this system in biobrick standard format. We have submitted two complementary systems: The first allows rapid testing of the system, but in a transient way. It also includes tools for debugging of the system. Once a working system is in place, it can be reassembled and integrated on the chromosome for longer-term use.<br />
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In order to be able to bring synthetic biology to multicellular organisms, it is essential that we have a communication device enabling cell-to-cell or tissue-to-tissue communication. We have advanced towards the implementation of the first synthetic hormone, which would allow communication between cells in an orthogonal manner. We have submitted an auxin receiver device to the registry, for use in combination with an auxin production system that we have adapted for eukaryotic chassis. <br />
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Merging the power of the Golden Gate multi-fragment cloning technique with the standardization of the Biobrick format. As a result, our team is developing this year a new parts format: the GoldenBricks. This is a new ultrafast cloning technique designed to assemble entire cassette in one shot. Ultimately, the GoldenBricks method is paving the way for future PartsRegistry formats!<br />
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Modeling a synthetic system at the whole organism scale is a challenge in itself. Using differential equations and agent based simulation, we aimed at modeling our entire synthetic hormonal system, as well as providing a new set of mathematical tool for iGEM in order to model complete organisms.<br />
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<h2 id="human">Human practice: What does it mean to be a chassis?</h2><br />
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Working with vertebrate embryos raised many questions among the team. We decided to track the changes in our attitude to animal experimentation during our project. Before starting our experiments, we answered a list of questions concerning animal experimentation and synthetic biology. Our reflection progressively lead us to realize that considering animals as mere chassis waiting to be engineered was quite a problematic conception that we had to question.<br />
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<h2 id="dummies">Xenopus for dummies: a guidebook for iGEMers</h2><br />
<p><br />
Xenopus is a quite complex organism we were the first team to work with it in iGEM. This is why we wrote a guidebook to help future teams find information and brainstorm for project with this organism, in the dummies format!<br />
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<h2 id="design"> style="text-decoration:none; color: white;"><b>Biotic games:</b> Better understanding of tadpole behavior through gaming</a></h2><br />
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</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/ProjectTeam:Evry/Project2012-10-26T23:51:19Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<br />
<!-- contents --><br />
<br />
<html><br />
<h1>Project overview</h1><br />
<p><br />
For our first participation in iGEM, we have decided to introduce a new organism to the competition: <i>Xenopus tropicalis</i>. Its common name is the Western clawed frog, a diploid cousin of the model organism <i>Xenopus laevis</i>. Aside for the soft spot French have for frogs, we also believe <i>Xenopus</i> could be a great multicellular chassis for synthetic biology. We are therefore bringing this organism to iGEM for the first time, along with the tools we need to bring Synthetic biology to the multicellular era.<br />
</p><br />
<br />
<p><br />
The laboratory part of our work can be divided into two categories: the creation of synthetic biology tools for <i>Xenopus</i> and the creation of a synthetic hormonal system. We created a multi-level model of this inter-tissue communication system, concurrently laying the groundwork for modeling of synthetic genetic systems in multicellular organisms. Finally, using vertebrates in synthetic biology poses deep ethical problems, which come alongside those of animal experimentation. iGEM aims to be cool and fun, but can we or should we keep the same attitude when working with vertebrate embryos ? Should we reduce animals to objects or tools by using words such as chassis when working with these multicellular organisms? Our resident philosopher lead our team’s reflection on these issues, proposing a guide for future synthetic biologists who wish to work with <i>Xenopus</i>. <br />
</p><br />
<br />
<div id="contourmenu" style="position: relative; margin-left: auto; margin-right: auto; width: 700px; text-align: left; background-color: #cee9f4; border-radius: 7px; align: left"><br />
<h3 style="text-align: center; padding-top:10px;">The French Froggies Project</h3><br><br />
<div id="item" style="margin-left:20px;"><br />
<ul><br />
<a href="#xenopus" style="text-decoration:none;"><li><i>Xenopus</i>: A new multicellular chassis</li></a><br />
<a href="#hormone" style="text-decoration:none;"><li>Intertissue communication: An orthogonal hormonal system </li></a><br />
<a href="#goldenBrick" style="text-decoration:none;"><li>GoldenBrick: A new biobrick cloning format</li></a><br />
<a href="#modeling" style="text-decoration:none;"><li>Modeling a tadpole: A multi-level approach</li></a><br />
<a href="#human" style="text-decoration:none;"><li>Human pratice: What does it mean to be a chassis?</a><br><br><br />
<a href="#dummies" style="text-decoration:none;"><li>Xenopus for dummies: an iGEMer guidebook</a><br><br><br />
</ul><br />
</div><br />
</font><br />
</div><br><br><br />
<br />
<!-- Bannière Grenouille --><br />
<center> <img src="http://image.noelshack.com/fichiers/2012/27/1341654981-gregre.gif" width=800 /> </center><br />
<!-- #Bannière Grenouille --><br />
<br />
<br><br><br><br />
<!-- Xenopus Tropicalis --><br />
<h2 id="xenopus"><i>Xenopus</i>: A new multicellular chassis</h2><br />
<br />
<p><br />
While iGEM has so far been mostly focused on en engineering unicellular organisms and bacteria in particular, we have decided work on the next step for synthetic biology: Multicellular engineering. Using our new biobrick tools, the road towards synthetic circuits in <i>Xenopus</i> is open, multiplying the number of potential applications in terms of synthetic biology.<br />
</p><br />
<br />
<p><br />
<i>Xenopus</i> is a model organism for developmental biology. Rapid development, easy handling and direct injection of plasmids into the fertilized egg allow testing of constructs in less than two weeks. We provide frog compatible plasmids for using this system in biobrick standard format. We have submitted two complementary systems: The first allows rapid testing of the system, but in a transient way. It also includes tools for debugging of the system. Once a working system is in place, it can be reassembled and integrated on the chromosome for longer-term use.<br />
</p><br />
<br />
<br><br />
<div id="contourmenu" class="moredetails"><br />
<table style="background:transparent";><br />
<tr><td><img style="padding-top:10px;padding-left:10px;" src="https://static.igem.org/mediawiki/2012/b/bd/Xenope.png" width=80px></td><br />
<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://2012.igem.org/Team:Evry/FrenchFrog"><center>More details here...</center></a><br />
<br />
</u></h3></td><br />
</tr></table><br />
<br /><br />
</font><br />
</div><br />
<br />
<!-- AID System --><br />
<h2 id="hormone">Intertissue communication: An orthogonal hormonal system </h2><br />
<p><br />
In order to be able to bring synthetic biology to multicellular organisms, it is essential that we have a communication device enabling cell-to-cell or tissue-to-tissue communication. We have advanced towards the implementation of the first synthetic hormone, which would allow communication between cells in an orthogonal manner. We have submitted an auxin receiver device to the registry, for use in combination with an auxin production system that we have adapted for eukaryotic chassis. <br />
</p><br />
<br />
<br />
<br><br />
<div id="contourmenu" class="moredetails" style="align:center"><br />
<br />
<table style="background:transparent";><br />
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<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://2012.igem.org/Team:Evry/AIDSystem"><center>More details here...</center></a><br />
<br />
</u></h3></td><br />
</tr></table><br />
<br /><br />
</font><br />
</div><br />
<br />
<br />
<!-- GoldenBrick --><br />
<h2 id="goldenBrick">GoldenBrick: A new biobrick cloning format</h2><br />
<p><br />
Merging the power of the Golden Gate multi-fragment cloning technique with the standardization of the Biobrick format. As a result, our team is developing this year a new parts format: the GoldenBricks. This is a new ultrafast cloning technique designed to assemble entire cassette in one shot. Ultimately, the GoldenBricks method is paving the way for future PartsRegistry formats!<br />
</p><br />
<br><br />
<div id="contourmenu" class="moredetails"><br />
<br />
<table style="background:transparent";><br />
<tr><td><img style="padding-top:10px;padding-left:10px;" src="https://static.igem.org/mediawiki/2012/a/a4/GoldeN.png" width=80px></td><br />
<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://2012.igem.org/Team:Evry/GB"><center>More details here...</center></a><br />
<br />
</u></h3></td><br />
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<br /><br />
</font><br />
</div><br />
<br />
<!-- Modeling --><br />
<h2 id="modeling">Modeling a tadpole: A multi-level approach</h2><br />
<p><br />
Modeling a synthetic system at the whole organism scale is a challenge in itself. Using differential equations and agent based simulation, we aimed at modeling our entire synthetic hormonal system, as well as providing a new set of mathematical tool for iGEM in order to model complete organisms.<br />
</p><br />
<br />
<br><br />
<div id="contourmenu" class="moredetails"><br />
<br />
<table style="background:transparent";><br />
<tr><td><img style="padding-top:10px;padding-left:10px;" src="https://static.igem.org/mediawiki/2012/5/5b/ModelingDHIUHEIUHD.png" width=80px></td><br />
<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://2012.igem.org/Team:Evry/Modeling"><center>More details here...</center></a><br />
<br />
</u></h3></td><br />
</tr></table><br />
<br /><br />
</font><br />
</div><br />
<br />
<br />
<!-- Human Practice --><br />
<h2 id="human">Human practice: What does it mean to be a chassis?</h2><br />
<p><br />
Working with vertebrate embryos raised many questions among the team. We decided to track the changes in our attitude to animal experimentation during our project. Before starting our experiments, we answered a list of questions concerning animal experimentation and synthetic biology. Our reflection progressively lead us to realize that considering animals as mere chassis waiting to be engineered was quite a problematic conception that we had to question.<br />
</p><br />
<br />
<br><br />
<div id="contourmenu" class="moredetails"><br />
<br />
<table style="background:transparent";><br />
<tr><td><img style="padding-top:10px;padding-left:10px;" src="https://static.igem.org/mediawiki/2012/b/ba/HumanPractice.png" width=80px></td><br />
<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://2012.igem.org/Team:Evry/HumanPractice"><center>More details here...</center></a><br />
<br />
</u></h3></td><br />
</tr></table><br />
<br /><br />
</font><br />
</div><br />
<br><br><br />
<br />
<!-- Frog for dummies --><br />
<h2 id="dummies">Xenopus for dummies: a guidebook for iGEMers</h2><br />
<p><br />
Xenopus is a quite complex organism we were the first team to work with it in iGEM. This is why we wrote a guidebook to help future teams find information and brainstorm for project with this organism, in the dummies format!<br />
</p><br />
<br />
<br><br />
<div id="contourmenu" class="moredetails"><br />
<br />
<table style="background:transparent";><br />
<tr><td><img style="padding-top:10px;padding-left:10px;" src="https://static.igem.org/mediawiki/2012/b/b9/FrogForDummies.png" width=80px></td><br />
<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://2012.igem.org/Team:Evry/FrogForDummies"><center>More details here...</center></a><br />
<br />
</u></h3></td><br />
</tr></table><br />
<br /><br />
</font><br />
</div><br />
<br><br><br />
<br />
<br />
<!--<br />
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<br /><br />
<p><a href="https://2012.igem.org/Team:Evry/Modeling">Visit the project page ></a></p><br />
<br /><br />
</font><br />
</div><br />
<br />
<h2 id="design"> style="text-decoration:none; color: white;"><b>Biotic games:</b> Better understanding of tadpole behavior through gaming</a></h2><br />
<p><a href="https://2012.igem.org/Team:Evry/BGame">Visit the project page ></a></p><br />
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</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/ProjectTeam:Evry/Project2012-10-26T23:50:36Z<p>Cyrpaut: </p>
<hr />
<div>{{:Team:Evry/template_v1}}<br />
<br />
<!-- contents --><br />
<br />
<html><br />
<h1>Project overview</h1><br />
<p><br />
For our first participation in iGEM, we have decided to introduce a new organism to the competition: <i>Xenopus tropicalis</i>. Its common name is the Western clawed frog, a diploid cousin of the model organism <i>Xenopus laevis</i>. Aside for the soft spot French have for frogs, we also believe <i>Xenopus</i> could be a great multicellular chassis for synthetic biology. We are therefore bringing this organism to iGEM for the first time, along with the tools we need to bring Synthetic biology to the multicellular era.<br />
</p><br />
<br />
<p><br />
The laboratory part of our work can be divided into two categories: the creation of synthetic biology tools for <i>Xenopus</i> and the creation of a synthetic hormonal system. We created a multi-level model of this inter-tissue communication system, concurrently laying the groundwork for modeling of synthetic genetic systems in multicellular organisms. Finally, using vertebrates in synthetic biology poses deep ethical problems, which come alongside those of animal experimentation. iGEM aims to be cool and fun, but can we or should we keep the same attitude when working with vertebrate embryos ? Should we reduce animals to objects or tools by using words such as chassis when working with these multicellular organisms? Our resident philosopher lead our team’s reflection on these issues, proposing a guide for future synthetic biologists who wish to work with <i>Xenopus</i>. <br />
</p><br />
<br />
<div id="contourmenu" style="position: relative; margin-left: auto; margin-right: auto; width: 700px; text-align: left; background-color: #cee9f4; border-radius: 7px; align: left"><br />
<h3 style="text-align: center; padding-top:10px;">The French Froggies Project</h3><br><br />
<div id="item" style="margin-left:20px;"><br />
<ul><br />
<a href="#xenopus" style="text-decoration:none;"><li><i>Xenopus</i>: A new multicellular chassis</li></a><br />
<a href="#hormone" style="text-decoration:none;"><li>Intertissue communication: An orthogonal hormonal system </li></a><br />
<a href="#goldenBrick" style="text-decoration:none;"><li>GoldenBrick: A new biobrick cloning format</li></a><br />
<a href="#modeling" style="text-decoration:none;"><li>Modeling a tadpole: A multi-level approach</li></a><br />
<a href="#human" style="text-decoration:none;"><li>Human pratice: What does it mean to be a chassis?</a><br><br><br />
</ul><br />
</div><br />
</font><br />
</div><br><br><br />
<br />
<!-- Bannière Grenouille --><br />
<center> <img src="http://image.noelshack.com/fichiers/2012/27/1341654981-gregre.gif" width=800 /> </center><br />
<!-- #Bannière Grenouille --><br />
<br />
<br><br><br><br />
<!-- Xenopus Tropicalis --><br />
<h2 id="xenopus"><i>Xenopus</i>: A new multicellular chassis</h2><br />
<br />
<p><br />
While iGEM has so far been mostly focused on en engineering unicellular organisms and bacteria in particular, we have decided work on the next step for synthetic biology: Multicellular engineering. Using our new biobrick tools, the road towards synthetic circuits in <i>Xenopus</i> is open, multiplying the number of potential applications in terms of synthetic biology.<br />
</p><br />
<br />
<p><br />
<i>Xenopus</i> is a model organism for developmental biology. Rapid development, easy handling and direct injection of plasmids into the fertilized egg allow testing of constructs in less than two weeks. We provide frog compatible plasmids for using this system in biobrick standard format. We have submitted two complementary systems: The first allows rapid testing of the system, but in a transient way. It also includes tools for debugging of the system. Once a working system is in place, it can be reassembled and integrated on the chromosome for longer-term use.<br />
</p><br />
<br />
<br><br />
<div id="contourmenu" class="moredetails"><br />
<table style="background:transparent";><br />
<tr><td><img style="padding-top:10px;padding-left:10px;" src="https://static.igem.org/mediawiki/2012/b/bd/Xenope.png" width=80px></td><br />
<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://2012.igem.org/Team:Evry/FrenchFrog"><center>More details here...</center></a><br />
<br />
</u></h3></td><br />
</tr></table><br />
<br /><br />
</font><br />
</div><br />
<br />
<!-- AID System --><br />
<h2 id="hormone">Intertissue communication: An orthogonal hormonal system </h2><br />
<p><br />
In order to be able to bring synthetic biology to multicellular organisms, it is essential that we have a communication device enabling cell-to-cell or tissue-to-tissue communication. We have advanced towards the implementation of the first synthetic hormone, which would allow communication between cells in an orthogonal manner. We have submitted an auxin receiver device to the registry, for use in combination with an auxin production system that we have adapted for eukaryotic chassis. <br />
</p><br />
<br />
<br />
<br><br />
<div id="contourmenu" class="moredetails" style="align:center"><br />
<br />
<table style="background:transparent";><br />
<tr><td><img style="padding-top:10px;padding-left:10px;" src="https://static.igem.org/mediawiki/2012/e/e2/Hormone.png" width=80px></td><br />
<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://2012.igem.org/Team:Evry/AIDSystem"><center>More details here...</center></a><br />
<br />
</u></h3></td><br />
</tr></table><br />
<br /><br />
</font><br />
</div><br />
<br />
<br />
<!-- GoldenBrick --><br />
<h2 id="goldenBrick">GoldenBrick: A new biobrick cloning format</h2><br />
<p><br />
Merging the power of the Golden Gate multi-fragment cloning technique with the standardization of the Biobrick format. As a result, our team is developing this year a new parts format: the GoldenBricks. This is a new ultrafast cloning technique designed to assemble entire cassette in one shot. Ultimately, the GoldenBricks method is paving the way for future PartsRegistry formats!<br />
</p><br />
<br><br />
<div id="contourmenu" class="moredetails"><br />
<br />
<table style="background:transparent";><br />
<tr><td><img style="padding-top:10px;padding-left:10px;" src="https://static.igem.org/mediawiki/2012/a/a4/GoldeN.png" width=80px></td><br />
<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://2012.igem.org/Team:Evry/GB"><center>More details here...</center></a><br />
<br />
</u></h3></td><br />
</tr></table><br />
<br /><br />
</font><br />
</div><br />
<br />
<!-- Modeling --><br />
<h2 id="modeling">Modeling a tadpole: A multi-level approach</h2><br />
<p><br />
Modeling a synthetic system at the whole organism scale is a challenge in itself. Using differential equations and agent based simulation, we aimed at modeling our entire synthetic hormonal system, as well as providing a new set of mathematical tool for iGEM in order to model complete organisms.<br />
</p><br />
<br />
<br><br />
<div id="contourmenu" class="moredetails"><br />
<br />
<table style="background:transparent";><br />
<tr><td><img style="padding-top:10px;padding-left:10px;" src="https://static.igem.org/mediawiki/2012/5/5b/ModelingDHIUHEIUHD.png" width=80px></td><br />
<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://2012.igem.org/Team:Evry/Modeling"><center>More details here...</center></a><br />
<br />
</u></h3></td><br />
</tr></table><br />
<br /><br />
</font><br />
</div><br />
<br />
<br />
<!-- Human Practice --><br />
<h2 id="human">Human practice: What does it mean to be a chassis?</h2><br />
<p><br />
Working with vertebrate embryos raised many questions among the team. We decided to track the changes in our attitude to animal experimentation during our project. Before starting our experiments, we answered a list of questions concerning animal experimentation and synthetic biology. Our reflection progressively lead us to realize that considering animals as mere chassis waiting to be engineered was quite a problematic conception that we had to question.<br />
</p><br />
<br />
<br><br />
<div id="contourmenu" class="moredetails"><br />
<br />
<table style="background:transparent";><br />
<tr><td><img style="padding-top:10px;padding-left:10px;" src="https://static.igem.org/mediawiki/2012/b/ba/HumanPractice.png" width=80px></td><br />
<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://2012.igem.org/Team:Evry/HumanPractice"><center>More details here...</center></a><br />
<br />
</u></h3></td><br />
</tr></table><br />
<br /><br />
</font><br />
</div><br />
<br><br><br />
<br />
<!-- Frog for dummies --><br />
<h2 id="dummies">Xenopus for dummies: a guidebook for iGEMers</h2><br />
<p><br />
Xenopus is a quite complex organism we were the first team to work with it in iGEM. This is why we wrote a guidebook to help future teams find information and brainstorm for project with this organism, in the dummies format!<br />
</p><br />
<br />
<br><br />
<div id="contourmenu" class="moredetails"><br />
<br />
<table style="background:transparent";><br />
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<td><br />
<h3 style="text-align:center; padding-left:20px;"></u><br />
<br />
<a href="https://2012.igem.org/Team:Evry/FrogForDummies"><center>More details here...</center></a><br />
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<h2 id="design"> style="text-decoration:none; color: white;"><b>Biotic games:</b> Better understanding of tadpole behavior through gaming</a></h2><br />
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</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/template.jsTeam:Evry/template.js2012-10-26T23:49:18Z<p>Cyrpaut: </p>
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</html></div>Cyrpauthttp://2012.igem.org/Team:Evry/main-img.jsTeam:Evry/main-img.js2012-10-26T23:34:00Z<p>Cyrpaut: </p>
<hr />
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if (document.referrer.match(/GoldenBricksxxxxxxxx|Plasmids|AIDSystem/)){<br />
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moved:function(e){<br />
var pos = $(canvas).offset();<br />
_mouseP = arbor.Point(e.pageX-pos.left, e.pageY-pos.top)<br />
nearest = sys.nearest(_mouseP);<br />
<br />
if (!nearest.node) return false<br />
<br />
// if (nearest.node.data.shape!='dot'){<br />
selected = (nearest.distance < 50) ? nearest : null<br />
if (selected){<br />
dom.addClass('linkable')<br />
window.status = selected.node.data.link//.replace(/^\//,"http://"+window.location.host+"/")//.replace(/^#/,'')<br />
}<br />
else{<br />
dom.removeClass('linkable')<br />
window.status = ''<br />
}<br />
// }else <br />
if ($.inArray(nearest.node.name, ['The French Froggies Project!','Xenopus as a chassis','Hormonal Communicati','Modelingxxxxxxxxxxxx','GoldenBricksxxxxxxxx', 'Human Practicexxxxxx', 'The Teamxxxxxxxxxxxx']) >=0 ){<br />
if (nearest.node.name!=_section){<br />
_section = nearest.node.name<br />
that.switchSection(_section)<br />
}<br />
dom.removeClass('linkable')<br />
window.status = ''<br />
}<br />
<br />
return false<br />
},<br />
clicked:function(e){<br />
var pos = $(canvas).offset();<br />
_mouseP = arbor.Point(e.pageX-pos.left, e.pageY-pos.top)<br />
nearest = dragged = sys.nearest(_mouseP);<br />
<br />
if (nearest && selected && nearest.node===selected.node){<br />
var link = selected.node.data.link<br />
if (link.match(/^#/)){<br />
$(that).trigger({type:"navigate", path:link.substr(1)})<br />
}else{<br />
window.location = link<br />
}<br />
return false<br />
}<br />
<br />
<br />
if (dragged && dragged.node !== null) dragged.node.fixed = true<br />
<br />
$(canvas).unbind('mousemove', handler.moved);<br />
$(canvas).bind('mousemove', handler.dragged)<br />
$(window).bind('mouseup', handler.dropped)<br />
<br />
return false<br />
},<br />
dragged:function(e){<br />
var old_nearest = nearest && nearest.node._id<br />
var pos = $(canvas).offset();<br />
var s = arbor.Point(e.pageX-pos.left, e.pageY-pos.top)<br />
<br />
if (!nearest) return<br />
if (dragged !== null && dragged.node !== null){<br />
var p = sys.fromScreen(s)<br />
dragged.node.p = p<br />
}<br />
<br />
return false<br />
},<br />
<br />
dropped:function(e){<br />
if (dragged===null || dragged.node===undefined) return<br />
if (dragged.node !== null) dragged.node.fixed = false<br />
dragged.node.tempMass = 1000<br />
dragged = null;<br />
// selected = null<br />
$(canvas).unbind('mousemove', handler.dragged)<br />
$(window).unbind('mouseup', handler.dropped)<br />
$(canvas).bind('mousemove', handler.moved);<br />
_mouseP = null<br />
return false<br />
}<br />
<br />
<br />
}<br />
<br />
$(canvas).mousedown(handler.clicked);<br />
$(canvas).mousemove(handler.moved);<br />
<br />
}<br />
}<br />
<br />
return that<br />
}<br />
<br />
<br />
var Nav = function(elt){<br />
var dom = $(elt)<br />
<br />
var _path = null<br />
<br />
var that = {<br />
init:function(){<br />
$(window).bind('popstate',that.navigate)<br />
dom.find('> a').click(that.back)<br />
$('.more').one('click',that.more)<br />
<br />
$('#Model dl:not(.datastructure) dt').click(that.reveal)<br />
that.update()<br />
return that<br />
},<br />
more:function(e){<br />
$(this).removeAttr('href').addClass('less').html('&nbsp;').siblings().fadeIn()<br />
$(this).next('h2').find('a').one('click', that.less)<br />
<br />
return false<br />
},<br />
less:function(e){<br />
var more = $(this).closest('h2').prev('a')<br />
$(this).closest('h2').prev('a')<br />
.nextAll().fadeOut(function(){<br />
$(more).text('creation & use').removeClass('less').attr('href','#')<br />
})<br />
$(this).closest('h2').prev('a').one('click',that.more)<br />
<br />
return false<br />
},<br />
reveal:function(e){<br />
$(this).next('dd').fadeToggle('fast')<br />
return false<br />
},<br />
back:function(){<br />
_path = "/"<br />
if (window.history && window.history.pushState){<br />
window.history.pushState({path:_path}, "", _path);<br />
}<br />
that.update()<br />
return false<br />
},<br />
navigate:function(e){<br />
var oldpath = _path<br />
if (e.type=='navigate'){<br />
_path = e.path<br />
if (window.history && window.history.pushState){<br />
window.history.pushState({path:_path}, "", _path);<br />
}else{<br />
that.update()<br />
}<br />
}else if (e.type=='popstate'){<br />
var state = e.originalEvent.state || {}<br />
_path = state.path || window.location.pathname.replace(/^\//,'')<br />
}<br />
if (_path != oldpath) that.update()<br />
},<br />
update:function(){<br />
var dt = 'slow'<br />
if (_path===null){<br />
// this is the original page load. don't animate anything just jump<br />
// to the proper state<br />
_path = window.location.pathname.replace(/^\//,'')<br />
dt = 0<br />
dom.find('p').css('opacity',0).show().fadeTo('slow',1)<br />
}<br />
<br />
switch (_path){<br />
case '':<br />
case '/':<br />
// dom.find('p').text('a graph visualization library using web workers and jQuery')<br />
dom.find('> a').removeClass('active').attr('href','#')<br />
<br />
$('#Model').fadeTo('fast',0, function(){<br />
$(this).hide()<br />
$(that).trigger({type:'mode', mode:'visible', dt:dt})<br />
})<br />
document.title = "The French Froggies Project!"<br />
break<br />
<br />
}<br />
<br />
}<br />
}<br />
return that<br />
}<br />
<br />
$(document).ready(function(){<br />
var CLR = {<br />
branch:"#b2b19d",<br />
level3benchwork:"#FFBF3E",<br />
level3model:"#4B86C7"<br />
}<br />
/*Thibault BEGIN*/<br />
var theUI = {<br />
<br />
// ['The French Froggies Project!','Xenopus as a chassis','Hormonal Communicati','Modelingxxxxxxxxxxxx','GoldenBricksxxxxxxxx', 'Human Practicexxxxxx', 'The Teamxxxxxxxxxxxx']<br />
<br />
nodes:{"The French Froggies Project!":{color:"#51C215", shape:"img", alpha:1, link:'https://2012.igem.org/Team:Evry/Project', url:'https://static.igem.org/mediawiki/2012/c/cf/French.png'}, //Attribut url pour l'image ajouté /*Thibault*/<br />
<br />
"Xenopus as a chassis":{color:"#63a358", shape:"img", alpha:1, link:'https://2012.igem.org/Team:Evry/FrenchFrog', url:'https://static.igem.org/mediawiki/2012/b/bd/Xenope.png'}, //Attribut url pour l'image ajouté /*Thibault*/<br />
"Xenopus plasmids":{color:"#63a358", alpha:0, link:'https://2012.igem.org/Team:Evry/FrenchFrog#plasmid'},<br />
"Injection in Xenopus":{color:"#63a358", alpha:0, link:'https://2012.igem.org/Team:Evry/InjectionTuto'},<br />
"Development stages":{color:"#63a358", alpha:0, link:'https://2012.igem.org/Team:Evry/Stages'},<br />
"Characterization":{color:"#63a358", alpha:0, link:'https://2012.igem.org/Team:Evry/Tadpole_injection1'},<br />
<br />
"Hormonal Communicati":{color:"#852c2b", shape:"img", alpha:1, link:'https://2012.igem.org/Team:Evry/AIDSystem', url:'https://static.igem.org/mediawiki/2012/e/e2/Hormone.png'}, //Attribut url pour l'image ajouté /*Thibault*/<br />
"Auxin emitter":{color:"#852c2b", alpha:0, link:'https://2012.igem.org/Team:Evry/AIDSystem#auxin'},<br />
"Auxin receiver":{color:"#852c2b", alpha:0, link:'https://2012.igem.org/Team:Evry/AIDSystem#AID'},<br />
"Auxin toxicity":{color:"#852c2b", alpha:0, link:'https://2012.igem.org/Team:Evry/AuxinTOX'},<br />
"Auxin uptake":{color:"#852c2b", alpha:0, link:'https://2012.igem.org/Team:Evry/auxin_uptake'},<br />
<br />
"Modelingxxxxxxxxxxxx":{color:"#1A5291", shape:"img", alpha:1, link:'https://2012.igem.org/Team:Evry/Modeling', url:'https://static.igem.org/mediawiki/2012/5/5b/ModelingDHIUHEIUHD.png'}, //Attribut url pour l'image ajouté /*Thibault*/<br />
"General Model":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/ODE_model'},<br />
"ODEs derivations":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/auxin_pde'},<br />
"Diffusion":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/Auxin_diffusion'},<br />
"Auxin production":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/auxin_production'},<br />
"Auxin reception":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/auxin_detection'},<br />
"Plasmid diffusion":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/plasmid_splitting'},<br />
"Model integration":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/model_integration'},<br />
"Parameters measurements":{color:CLR.level3model, alpha:0, link:'https://2012.igem.org/Team:Evry/Experimentals_Parameters'},<br />
<br />
"GoldenBricksxxxxxxxx":{color:"#1A5291", shape:"img", alpha:1, link:'https://2012.igem.org/Team:Evry/GB', url:'https://static.igem.org/mediawiki/2012/a/a4/GoldeN.png'}, //Attribut url pour l'image ajouté /*Thibault*/<br />
<br />
"Human Practicexxxxxx":{color:"#a6953f", shape:"img", alpha:1, link:'https://2012.igem.org/Team:Evry/HumanPractice', url:'https://static.igem.org/mediawiki/2012/b/ba/HumanPractice.png'}, //Attribut url pour l'image ajouté /*Thibault*/<br />
"Hi Xenope!":{color:"#a6953f", alpha:0, link:'https://2012.igem.org/Team:Evry/HumanPractice/Introduction'},<br />
"Be a chassis?":{color:"#a6953f", alpha:0, link:'https://2012.igem.org/Team:Evry/HumanPractice/modelorganism'},<br />
"Free the frogs!":{color:"#a6953f", alpha:0, link:'https://2012.igem.org/Team:Evry/HumanPractice/freedthefrogs'},<br />
"A chassis, really?":{color:"#a6953f", alpha:0, link:'https://2012.igem.org/Team:Evry/HumanPractice/chassis'},<br />
"Work with Xenopus again?":{color:"#a6953f", alpha:0, link:'https://2012.igem.org/Team:Evry/HumanPractice/future'},<br />
"What the laws says...":{color:"#a6953f", alpha:0, link:'https://2012.igem.org/Team:Evry/HumanPractice/others'},<br />
<br />
"The Teamxxxxxxxxxxxx":{color:"#632e8c", shape:"img", alpha:1, link:'https://2012.igem.org/Team:Evry/Team', url:'https://static.igem.org/mediawiki/2012/9/93/TeamEVRY.png'}, //Attribut url pour l'image ajouté /*Thibault*/<br />
"Our Team":{color:"#632e8c", alpha:0, link:'https://2012.igem.org/Team:Evry/Team'},<br />
"Our Sponsors":{color:"#632e8c", alpha:0, link:'https://2012.igem.org/Team:Evry/Sponsors'},<br />
"Attributions":{color:"#632e8c", alpha:0, link:'https://2012.igem.org/Team:Evry/Attributions'},<br />
"Official profile":{color:"#632e8c", alpha:0, link:'https://igem.org/Team.cgi?year=2012'},<br />
"Collaborations":{color:"#632e8c", alpha:0, link:'https://2012.igem.org/Team:Evry/Collaborations'}<br />
},<br />
<br />
edges:{<br />
<br />
<br />
"The French Froggies Project!":{<br />
"Xenopus as a chassis":{"length":0.8,"weight":2},<br />
"Hormonal Communicati":{"length":0.8,"weight":2},<br />
"Modelingxxxxxxxxxxxx":{"length":0.8,"weight":2},<br />
"GoldenBricksxxxxxxxx":{"length":1.5,"weight":2},<br />
"Human Practicexxxxxx":{"length":0.75,"weight":2},<br />
"The Teamxxxxxxxxxxxx":{"length":0.5,"weight":2}<br />
},<br />
<br />
<br />
"Xenopus as a chassis":{<br />
"Xenopus plasmids":{"length":0.75,"weight":1},<br />
"Injection in Xenopus":{"length":0.75,"weight":2},<br />
"Development stages":{"length":0.75,"weight":2},<br />
"Characterization":{"length":0.75,"weight":2}<br />
},<br />
"Hormonal Communicati":{<br />
"Auxin emitter":{"length":1,"weight":2},<br />
"Auxin receiver":{"length":1,"weight":2},<br />
"Auxin toxicity":{"length":1,"weight":2},<br />
"Auxin uptake":{"length":1,"weight":2}<br />
<br />
},<br />
<br />
"Modelingxxxxxxxxxxxx":{<br />
"General Model":{"length":0.5,"weight":2},<br />
"ODEs derivations":{"length":0.5,"weight":2},<br />
"Diffusion":{"length":0.75,"weight":2},<br />
"Auxin production":{"length":0.5,"weight":2},<br />
"Auxin reception":{"length":0.5,"weight":2},<br />
"Plasmid diffusion":{"length":0.5,"weight":2},<br />
"Model integration":{"length":0.5,"weight":2},<br />
"Parameters measurements":{"length":0.5,"weight":2}<br />
},<br />
<br />
"GoldenBricksxxxxxxxx":{},<br />
<br />
<br />
"Human Practicexxxxxx":{<br />
"Hi Xenope!":{"length":0.5,"weight":2},<br />
"Be a chassis?":{"length":0.5,"weight":2},<br />
"Free the frogs!":{"length":0.5,"weight":2},<br />
"A chassis, really?":{"length":0.5,"weight":2},<br />
"Work with Xenopus again?":{"length":0.5,"weight":2},<br />
"What the laws says...":{"length":0.5,"weight":2}<br />
},<br />
<br />
"The Teamxxxxxxxxxxxx":{<br />
"Our Team":{"length":0.5,"weight":2},<br />
"Our Sponsors":{"length":0.5,"weight":2},<br />
"Attributions":{"length":0.5,"weight":2},<br />
"Official profile":{"length":0.5,"weight":2},<br />
"Collaborations":{"length":0.5,"weight":2}<br />
}<br />
}<br />
}<br />
/*Thibault END*/<br />
<br />
var sys = arbor.ParticleSystem()<br />
sys.parameters({stiffness:900, repulsion:50, gravity:true, dt:0.015})<br />
sys.renderer = Renderer("#sitemap")<br />
sys.graft(theUI)<br />
<br />
var nav = Nav("#nav")<br />
$(sys.renderer).bind('navigate', nav.navigate)<br />
$(nav).bind('mode', sys.renderer.switchMode)<br />
nav.init()<br />
})<br />
})(this.jQuery)</div>Cyrpaut