http://2012.igem.org/wiki/index.php?title=Special:Contributions/Csvidal&feed=atom&limit=50&target=Csvidal&year=&month=2012.igem.org - User contributions [en]2024-03-28T08:37:54ZFrom 2012.igem.orgMediaWiki 1.16.0http://2012.igem.org/Team:UC_Chile/Cyanolux/Results_shortTeam:UC Chile/Cyanolux/Results short2012-10-27T04:01:59Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Construction</h1><br />
<h2>Plasmids backbones</h2><br />
<br />
<br />
We built two integrative plasmid backbones for Synechocystis PCC. 6803 (see design [https://2012.igem.org/Team:UC_Chile/Cyanolux/Project_short#Wetlab_strategy here]) <br />
<br />
[[File:UC_Chile-IntSplasmidResult.jpg| 560px | right]]<br />
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<br /><br />
<br />
[[File:UC_Chile-IntKplasmidResult.jpg| 400px | left]]<br />
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<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
Plasmids were verified by digestion and then corroborated by sequencing.<br />
<br />
<br />
<h2>Constructs for Synechocystis</h2><br />
<br />
<br />
Using our plasmid backbones as a starting point, we proceeded to build our constructs for <i>Synechocystis</i>:<br />
<br />
<br />
[[File:UC_Chile-IntKfullplasmidResult.jpg| 640px]]<br />
<br /><br />
<br /><br />
Even though we tried many times (>6) to assemble the substrate production (LuxCDEG) plasmid,we were unable to obtain a correct product. We are currently designing another strategy to build the construct...<br />
<br />
<br /><br />
<br /><br />
<br />
<h1>Characterization in Synechocystis</h1><br />
<br />
<br /><br />
<br />
<h2>Transformation</h2><br />
<br />
<br />
We used our plasmid backbones to transform Synechocystis PCC. 6803 to verify that our initial design could indeed integrate into <i>Synechocystis's</i> genome.<br />
<br />
<br />
Transformation with pSB1C3_IntK (BBa_K743015):<br />
<br />
<br />
[[File:Pta_LuxABvf.JPG| 600px| center]]<br />
<br />
<br />
Transformation with pSB1C3_IntS (BBa_K743010):<br />
<br />
<br />
[[File:Trans_IntS.JPG| 600px| center]]<br />
<br />
<br />
After two weeks, colonies became apparent in the transformation plates for both plasmid backbones while in negative controls (transformations with no DNA) there was complete absence of surviving colonies.<br />
<br />
<br />
<h2>Verification of constructs</h2><br />
<br />
<br />
We proceeded to verify the integration of the constructs in <i>Synechocystis</i> by doing multiple PCRs to amplify various parts.<br />
<br />
[[File: UC_Chile-verification.jpg]]<br />
<br />
<br />
<h2>Bioluminescence Assays</h2><br />
<br />
We proceeded to check if our <i>Synechocystis</i> strain with the Luciferase genes did indeed produce light in the prescence of exogenous substrate.<br />
<br />
<h3>Bioluminometer</h3><br />
<br />
We measured bioluminescence by adding directly the substrate to the cells and measuring light-output in a Luminometer. <br />
<br />
<br />
<br /><br />
<html><center><img src="https://static.igem.org/mediawiki/2012/6/6a/Kjasfkjhasdf.jpg" align="right" width="600"></center></html><br />
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<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
<br />
While we could measure bioluminescence of the positive LuxBrick E.coli controls, no apparent bioluminescence was seen in our <i>Synechocystis</i> cells. This led us to think that the problem might be the size of the promoter, which if not long enough, would not be able to recruit necessary transcription factors for expression.<br />
<br />
<br />
<h3>High-sensitive camera</h3><br />
<br />
<br />
During the Latin America Jamboree, we had a chat with a couple of judges and a student from Universidad de los Andes, David Olarte, on how to induce Synechocystis with n-decanal.<br />
<br />
David sent us some work he had done on luminescence assays in cyanobacteria. Following his methods we finally were able to see light emission, confirming presence of catalytically active luciferase. Thanks David!<br />
<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/7/7f/Cam.results.finalisimo.jpg" align="right" width="600"></center></html><br />
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<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
<br />
<br />
<h1>Experimental Highlights</h1><br />
<br />
<br /><br />
<br /><br />
- Constructed two functional integration plasmids for <i>Synechocystis</i> PCC. 6803<br />
<br /><br />
<br /><br />
- Transformed <i>Synechocystis</i> PCC. 6803 with an optimized transformation protocol, available [https://2012.igem.org/Team:UC_Chile/Protocols#Transformation_of_Synechocystis_PCC._6803 here]<br />
<br /><br />
<br /><br />
- Verified integration of constructs<br />
<br /><br />
<br /><br />
- Confirmed expression of genes and activity of luciferase through a bioluminescence assay with exogenous substrate.<br />
<br /><br />
<br /><br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Results_shortTeam:UC Chile/Cyanolux/Results short2012-10-27T04:01:19Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Construction</h1><br />
<h2>Plasmids backbones</h2><br />
<br />
<br />
We built two integrative plasmid backbones for Synechocystis PCC. 6803 (see design [https://2012.igem.org/Team:UC_Chile/Cyanolux/Project_short#Wetlab_strategy here]) <br />
<br />
[[File:UC_Chile-IntSplasmidResult.jpg| 560px | right]]<br />
<br /><br />
<br /><br />
<br />
[[File:UC_Chile-IntKplasmidResult.jpg| 400px | left]]<br />
<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
Plasmids were verified by digestion and then corroborated by sequencing.<br />
<br />
<br />
<h2>Constructs for Synechocystis</h2><br />
<br />
<br />
Using our plasmid backbones as a starting point, we proceeded to build our constructs for <i>Synechocystis</i>:<br />
<br />
<br />
[[File:UC_Chile-IntKfullplasmidResult.jpg| 640px]]<br />
<br /><br />
<br /><br />
Even though we tried many times (>6) to assemble the substrate production (LuxCDEG) plasmid,we were unable to obtain a correct product. We are currently designing another strategy to build the construct...<br />
<br />
<br /><br />
<br /><br />
<br />
<h1>Characterization in Synechocystis</h1><br />
<br />
<br /><br />
<br />
<h2>Transformation</h2><br />
<br />
<br />
We used our plasmid backbones to transform Synechocystis PCC. 6803 to verify that our initial design could indeed integrate into <i>Synechocystis's</i> genome.<br />
<br />
<br />
Transformation with pSB1C3_IntK (BBa_K743015):<br />
<br />
<br />
[[File:Pta_LuxABvf.JPG| 600px| center]]<br />
<br />
<br />
Transformation with pSB1C3_IntS (BBa_K743010):<br />
<br />
<br />
[[File:Trans_IntS.JPG| 600px| center]]<br />
<br />
<br />
After two weeks, colonies became apparent in the transformation plates for both plasmid backbones while in negative controls (transformations with no DNA) there was complete absence of surviving colonies.<br />
<br />
<br />
<h2>Verification of constructs</h2><br />
<br />
<br />
We proceeded to verify the integration of the constructs in <i>Synechocystis</i> by doing multiple PCRs to amplify various parts.<br />
<br />
[[File: UC_Chile-verification.jpg]]<br />
<br />
<br />
<h2>Bioluminescence Assays</h2><br />
<br />
We proceeded to check if our <i>Synechocystis</i> strain with the Luciferase genes did indeed produce light in the prescence of exogenous substrate.<br />
<br />
<h3>Bioluminometer</h3><br />
<br />
We measured bioluminescence by adding directly the substrate to the cells and measuring light-output in a Luminometer. <br />
<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<html><center><img src="https://static.igem.org/mediawiki/2012/6/6a/Kjasfkjhasdf.jpg" align="right" width="600"></center></html><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
<br />
While we could measure bioluminescence of the positive LuxBrick E.coli controls, no apparent bioluminescence was seen in our <i>Synechocystis</i> cells. This led us to think that the problem might be the size of the promoter, which if not long enough, would not be able to recruit necessary transcription factors for expression.<br />
<br />
<br />
<h3>High-sensitive camera</h3><br />
<br />
<br />
During the Latin America Jamboree, we had a chat with a couple of judges and a student from Universidad de los Andes, David Olarte, on how to induce Synechocystis with n-decanal.<br />
<br />
David sent us some work he had done on luminescence assays in cyanobacteria. Following his methods we finally were able to see light emission, confirming presence of catalytically active luciferase. Thanks David!<br />
<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/7/7f/Cam.results.finalisimo.jpg" align="right" width="600"></center></html><br />
<br />
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<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
<br />
<br />
<h1>Experimental Highlights</h1><br />
<br />
<br /><br />
<br /><br />
- Constructed two functional integration plasmids for <i>Synechocystis</i> PCC. 6803<br />
<br /><br />
<br /><br />
- Transformed <i>Synechocystis</i> PCC. 6803 with an optimized transformation protocol, available [https://2012.igem.org/Team:UC_Chile/Protocols#Transformation_of_Synechocystis_PCC._6803 here]<br />
<br /><br />
<br /><br />
- Verified integration of constructs<br />
<br /><br />
<br /><br />
- Confirmed expression of genes and activity of luciferase through a bioluminescence assay with exogenous substrate.<br />
<br /><br />
<br /><br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Results_shortTeam:UC Chile/Cyanolux/Results short2012-10-27T04:00:07Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Construction</h1><br />
<h2>Plasmids backbones</h2><br />
<br />
<br />
We built two integrative plasmid backbones for Synechocystis PCC. 6803 (see design [https://2012.igem.org/Team:UC_Chile/Cyanolux/Project_short#Wetlab_strategy here]) <br />
<br />
[[File:UC_Chile-IntSplasmidResult.jpg| 560px | right]]<br />
<br /><br />
<br /><br />
<br />
[[File:UC_Chile-IntKplasmidResult.jpg| 400px | left]]<br />
<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
Plasmids were verified by digestion and then corroborated by sequencing.<br />
<br />
<br />
<h2>Constructs for Synechocystis</h2><br />
<br />
<br />
Using our plasmid backbones as a starting point, we proceeded to build our constructs for <i>Synechocystis</i>:<br />
<br />
<br />
[[File:UC_Chile-IntKfullplasmidResult.jpg| 640px]]<br />
<br /><br />
<br /><br />
Even though we tried many times (>6) to assemble the substrate production (LuxCDEG) plasmid,we were unable to obtain a correct product. We are currently designing another strategy to build the construct...<br />
<br />
<br /><br />
<br /><br />
<br />
<h1>Characterization in Synechocystis</h1><br />
<br />
<br /><br />
<br />
<h2>Transformation</h2><br />
<br />
<br />
We used our plasmid backbones to transform Synechocystis PCC. 6803 to verify that our initial design could indeed integrate into <i>Synechocystis's</i> genome.<br />
<br />
<br />
Transformation with pSB1C3_IntK (BBa_K743015):<br />
<br />
<br />
[[File:Pta_LuxABvf.JPG| 600px| center]]<br />
<br />
<br />
Transformation with pSB1C3_IntS (BBa_K743010):<br />
<br />
<br />
[[File:Trans_IntS.JPG| 600px| center]]<br />
<br />
<br />
After two weeks, colonies became apparent in the transformation plates for both plasmid backbones while in negative controls (transformations with no DNA) there was complete absence of surviving colonies.<br />
<br />
<br />
<h2>Verification of constructs</h2><br />
<br />
<br />
We proceeded to verify the integration of the constructs in <i>Synechocystis</i> by doing multiple PCRs to amplify various parts.<br />
<br />
[[File: UC_Chile-verification.jpg]]<br />
<br />
<br />
<h2>Bioluminescence Assays</h2><br />
<br />
We proceeded to check if our <i>Synechocystis</i> strain with the Luciferase genes did indeed produce light in the prescence of exogenous substrate.<br />
<br />
<h3>Bioluminometer</h3><br />
<br />
We measured bioluminescence by adding directly the substrate to the cells and measuring light-output in a Luminometer. <br />
<br />
<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/6/6a/Kjasfkjhasdf.jpg" align="right" width="600"></center></html><br />
<br />
While we could measure bioluminescence of the positive LuxBrick E.coli controls, no apparent bioluminescence was seen in our <i>Synechocystis</i> cells. This led us to think that the problem might be the size of the promoter, which if not long enough, would not be able to recruit necessary transcription factors for expression.<br />
<br />
<br />
<h3>High-sensitive camera</h3><br />
<br />
<br />
During the Latin America Jamboree, we had a chat with a couple of judges and a student from Universidad de los Andes, David Olarte, on how to induce Synechocystis with n-decanal.<br />
<br />
David sent us some work he had done on luminescence assays in cyanobacteria. Following his methods we finally were able to see light emission, confirming presence of catalytically active luciferase. Thanks David!<br />
<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/7/7f/Cam.results.finalisimo.jpg" align="right" width="600"></center></html><br />
<br />
<br /><br />
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<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
<br />
<br />
<h1>Experimental Highlights</h1><br />
<br />
<br /><br />
<br /><br />
- Constructed two functional integration plasmids for <i>Synechocystis</i> PCC. 6803<br />
<br /><br />
<br /><br />
- Transformed <i>Synechocystis</i> PCC. 6803 with an optimized transformation protocol, available [https://2012.igem.org/Team:UC_Chile/Protocols#Transformation_of_Synechocystis_PCC._6803 here]<br />
<br /><br />
<br /><br />
- Verified integration of constructs<br />
<br /><br />
<br /><br />
- Confirmed expression of genes and activity of luciferase through a bioluminescence assay with exogenous substrate.<br />
<br /><br />
<br /><br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/FutureTeam:UC Chile/Cyanolux/Future2012-10-27T03:43:59Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Project status</h1><br />
<br />
Currently, we have been able to detect light-emission from our <i>Synechocystis</i> cells, however we still need to analyze circadian expression of the genes. Furthermore, we still have not been able to assemble the substrate production enzymes to the susceptibility plasmid. We hope that with out new strategy we will be successful. Once we are able to integrate the substrate production genes we may be able to assess the desired behavior of our system and analyze its impact on the chassis. <br />
<br />
After having the complete system we will have to see if we can achieve visible lighting with <i>Luxilla</i>. If not, we will try optimizing codon usage for Synechocystis and/or changing promoters.<br />
<br />
<h1>Biological lighting</h1><br />
<br />
Synechocystis PCC6803 has been proposed as the green E. coli. This chassis can be used in industry to achieve more sustainable processes.<br />
<br />
<h1>Context in Synbio</h1><br />
<br />
Since we started developing this project we have realized the importance of biological context, specially when functionality is a desired aspect of the behavioral output of the system. While in one chassis a Biobrick may provide a novel function, in another it may become a practical application for society.<br />
<br />
We have seen first hand the difficulties associated with this aspect of current progress in SynBio. By lacking standarized and proven methodologies and relying on previous uncharacterized work. We urge the Synbio community to address this aspect by encouraging the use and characterization of different chassis. It is of utmost importance that BioBrick designed for the chassis must be characterized in the chassis or they may prove fallible. The same can be said about failed experiences with BioBricks that are not reported.<br />
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<html><br />
<center> <iframe src="http://www.youtube.com/embed/Je5JkoOMITE?rel=0;3&amp;autohide=1&amp;showinfo=0" frameborder="0" width="853" height="480"></iframe> </center><br />
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<a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
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{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/FutureTeam:UC Chile/Cyanolux/Future2012-10-27T03:38:34Z<p>Csvidal: Undo revision 297866 by Csvidal (talk)</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Project status</h1><br />
<br />
Currently, we have been able to detect light-emission from our <i>Synechocystis</i> cells, however we still need to analyze circadian expression of the genes. Furthermore, we still have not been able to assemble the substrate production enzymes to the susceptibility plasmid. We hope that with out new strategy we will be successful. Once we are able to integrate the substrate production genes we may be able to assess the desired behavior of our system and analyze its impact on the chassis. <br />
<br />
After having the complete system we will have to see if we can achieve visible lighting with <i>Luxilla</i>. If not, we will try optimizing codon usage for Synechocystis and/or changing promoters.<br />
<br />
<h1>Biological lighting</h1><br />
<br />
Energy requirements keep on increasing. Resource consumption and dependence on fossil fuels has led modern society to use crops for fuel production. <br />
<br />
Sustainability is needed desperately, and we believe that <i>Luxilla</i> might bring some aid in that aspect. <br />
<br />
We have thought about real practical applications of <i>Luxilla</i> on passive lighting, specially in areas of transit like corridors or sidewalks. <br />
<br />
<h1>Context in Synbio</h1><br />
<br />
Since we started developing this project we have realized the importance of biological context, specially when functionality is a desired aspect of the behavioral output of the system. While in one chassis a Biobrick may provide a novel function, in another it may become a practical application for society.<br />
<br />
We have seen first hand the difficulties associated with this aspect of current progress in SynBio. (JAJAJ acabo de escribir SyneBIO). By lacking standarized and proven methodologies and relying on previous uncharacterized work. We urge the Synbio community to address this aspect by encouraging the use and characterization of different chassis. It is of utmost importance that BioBrick designed for the chassis must be characterized in the chassis or they may prove fallible. The same can be said about failed experiences with BioBricks that are not reported.<br />
<br />
<br />
<br />
<html><br />
<center> <iframe src="http://www.youtube.com/embed/Je5JkoOMITE?rel=0;3&amp;autohide=1&amp;showinfo=0" frameborder="0" width="853" height="480"></iframe> </center><br />
</html><br />
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<a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
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{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/FutureTeam:UC Chile/Cyanolux/Future2012-10-27T03:38:11Z<p>Csvidal: Undo revision 297847 by Csvidal (talk)</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Project status</h1><br />
<br />
Currently, we have been able to detect light-emission from our <i>Synechocystis</i> cells, however we still need to analyze circadian expression of the genes. Furthermore, we still have not been able to assemble the substrate production enzymes to the susceptibility plasmid. We hope that with out new strategy we will be successful. Once we are able to integrate the substrate production genes we may be able to assess the desired behavior of our system and analyze its impact on the chassis. <br />
<br />
After having the complete system we will have to see if we can achieve visible lighting with <i>Luxilla</i>. If not, we will try optimizing codon usage for Synechocystis and/or changing promoters.<br />
<br />
<h1>Biological lighting</h1><br />
<br />
Synechocystis PCC6803 has been proposed as the green E. coli. This chassis can be used in industry to achieve more sustainable processes.<br />
<br />
<br />
<h1>Context in Synbio</h1><br />
<br />
Since we started developing this project we have realized the importance of biological context, specially when functionality is a desired aspect of the behavioral output of the system. While in one chassis a Biobrick may provide a novel function, in another it may become a practical application for society.<br />
<br />
We have seen first hand the difficulties associated with this aspect of current progress in SynBio. By lacking standarized and proven methodologies and relying on previous uncharacterized work. We urge the Synbio community to address this aspect by encouraging the use and characterization of different chassis. It is of utmost importance that BioBrick designed for the chassis must be characterized in the chassis or they may prove fallible. The same can be said about failed experiences with BioBricks that are not reported.<br />
<br />
<br />
<br />
<br />
<br />
<html><br />
<center> <iframe src="http://www.youtube.com/embed/Je5JkoOMITE?rel=0;3&amp;autohide=1&amp;showinfo=0" frameborder="0" width="853" height="480"></iframe> </center><br />
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<a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
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{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/FutureTeam:UC Chile/Cyanolux/Future2012-10-27T03:37:51Z<p>Csvidal: Undo revision 297832 by Csvidal (talk)</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Project status</h1><br />
<br />
Currently, we have been able to detect light-emission from our <i>Synechocystis</i> cells, however we still need to analyze circadian expression of the genes. Furthermore, we still have not been able to assemble the substrate production enzymes to the susceptibility plasmid. We hope that with out new strategy we will be successful. Once we are able to integrate the substrate production genes we may be able to assess the desired behavior of our system and analyze its impact on the chassis. <br />
<br />
After having the complete system we will have to see if we can achieve visible lighting with <i>Luxilla</i>. If not, we will try optimizing codon usage for Synechocystis and/or changing promoters.<br />
<br />
<h1>Biological lighting</h1><br />
<br />
Energy requirements keep on increasing. Resource consumption and dependence on fossil fuels has led modern society to use crops for fuel production. <br />
<br />
Sustainability is needed desperately, and we believe that <i>Luxilla</i> might bring some aid in that aspect. <br />
<br />
We have thought about real practical applications of <i>Luxilla</i> on passive lighting, specially in areas of transit like corridors or sidewalks. <br />
<br />
<h1>Context in Synbio</h1><br />
<br />
Since we started developing this project we have realized the importance of biological context, specially when functionality is a desired aspect of the behavioral output of the system. While in one chassis a Biobrick may provide a novel function, in another it may become a practical application for society.<br />
<br />
We have seen first hand the difficulties associated with this aspect of current progress in SynBio. (JAJAJ acabo de escribir SyneBIO). By lacking standarized and proven methodologies and relying on previous uncharacterized work. We urge the Synbio community to address this aspect by encouraging the use and characterization of different chassis. It is of utmost importance that BioBrick designed for the chassis must be characterized in the chassis or they may prove fallible. The same can be said about failed experiences with BioBricks that are not reported.<br />
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<html><br />
<center> <iframe src="http://www.youtube.com/embed/Je5JkoOMITE?rel=0;3&amp;autohide=1&amp;showinfo=0" frameborder="0" width="853" height="480"></iframe> </center><br />
</html><br />
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<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
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{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/FutureTeam:UC Chile/Cyanolux/Future2012-10-27T03:37:21Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Project status</h1><br />
<br />
Currently, we have been able to detect light-emission from our <i>Synechocystis</i> cells, however we still need to analyze circadian expression of the genes. Furthermore, we still have not been able to assemble the substrate production enzymes to the susceptibility plasmid. We hope that with out new strategy we will be successful. Once we are able to integrate the substrate production genes we may be able to assess the desired behavior of our system and analyze its impact on the chassis. <br />
<br />
After having the complete system we will have to see if we can achieve visible lighting with <i>Luxilla</i>. If not, we will try optimizing codon usage for Synechocystis and/or changing promoters.<br />
<br />
<h1>Biological lighting</h1><br />
<br />
Synechocystis PCC6803 has been proposed as the green E. coli. This chassis can be used in industry to achieve more sustainable processes.<br />
<br />
<br />
<h1>Context in Synbio</h1><br />
<br />
Since we started developing this project we have realized the importance of biological context, specially when functionality is a desired aspect of the behavioral output of the system. While in one chassis a Biobrick may provide a novel function, in another it may become a practical application for society.<br />
<br />
We have seen first hand the difficulties associated with this aspect of current progress in SynBio. By lacking standarized and proven methodologies and relying on previous uncharacterized work. We urge the Synbio community to address this aspect by encouraging the use and characterization of different chassis. It is of utmost importance that BioBrick designed for the chassis must be characterized in the chassis or they may prove fallible. The same can be said about failed experiences with BioBricks that are not reported.<br />
<br />
<br />
<br />
<br />
<br />
<html><br />
<center> <iframe src="http://www.youtube.com/embed/Je5JkoOMITE?rel=0;3&amp;autohide=1&amp;showinfo=0" frameborder="0" width="853" height="480"></iframe> </center><br />
</html><br />
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<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
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{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Results_shortTeam:UC Chile/Cyanolux/Results short2012-10-27T03:22:57Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Construction</h1><br />
<h2>Plasmids backbones</h2><br />
<br />
<br />
We built two integrative plasmid backbones for Synechocystis PCC. 6803 (see design [https://2012.igem.org/Team:UC_Chile/Cyanolux/Project_short#Wetlab_strategy here]) <br />
<br />
[[File:UC_Chile-IntSplasmidResult.jpg| 560px | right]]<br />
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[[File:UC_Chile-IntKplasmidResult.jpg| 400px | left]]<br />
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<br /><br />
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<br /><br />
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<br /><br />
<br /><br />
<br /><br />
Plasmids were verified by digestion and then corroborated by sequencing.<br />
<br />
<br />
<h2>Constructs for Synechocystis</h2><br />
<br />
<br />
Using our plasmid backbones as a starting point, we proceeded to build our constructs for <i>Synechocystis</i>:<br />
<br />
<br />
[[File:UC_Chile-IntKfullplasmidResult.jpg| 640px]]<br />
<br /><br />
<br /><br />
Even though we tried many times (>6) to assemble the substrate production (LuxCDEG) plasmid,we were unable to obtain a correct product. We are currently designing another strategy to build the construct...<br />
<br />
<br /><br />
<br /><br />
<br />
<h1>Characterization in Synechocystis</h1><br />
<br />
<br /><br />
<br />
<h2>Transformation</h2><br />
<br />
<br />
We used our plasmid backbones to transform Synechocystis PCC. 6803 to verify that our initial design could indeed integrate into <i>Synechocystis's</i> genome.<br />
<br />
<br />
Transformation with pSB1C3_IntK (BBa_K743015):<br />
<br />
<br />
[[File:Pta_LuxABvf.JPG| 600px| center]]<br />
<br />
<br />
Transformation with pSB1C3_IntS (BBa_K743010):<br />
<br />
<br />
[[File:Trans_IntS.JPG| 600px| center]]<br />
<br />
<br />
After two weeks, colonies became apparent in the transformation plates for both plasmid backbones while in negative controls (transformations with no DNA) there was complete absence of surviving colonies.<br />
<br />
<br />
<h2>Verification of constructs</h2><br />
<br />
<br />
We proceeded to verify the integration of the constructs in <i>Synechocystis</i> by doing multiple PCRs to amplify various parts.<br />
<br />
[[File: UC_Chile-verification.jpg]]<br />
<br />
<br />
<h2>Bioluminescence Assays</h2><br />
<br />
We proceeded to check if our <i>Synechocystis</i> strain with the Luciferase genes did indeed produce light in the prescence of exogenous substrate.<br />
<br />
<h3>Bioluminometer</h3><br />
<br />
We measured bioluminescence by adding directly the substrate to the cells and measuring light-output in a Luminometer. <br />
<br />
[[File: UC_Chile-decanalgraph.jpg]]<br />
<br />
While we could measure bioluminescence of the positive LuxBrick E.coli controls, no apparent bioluminescence was seen in our <i>Synechocystis</i> cells. This led us to think that the problem might be the size of the promoter, which if not long enough, would not be able to recruit necessary transcription factors for expression.<br />
<br />
<br />
<h3>High-sensitive camera</h3><br />
<br />
<br />
During the Latin America Jamboree, we had a chat with a couple of judges and a student from Universidad de los Andes, David Olarte, on how to induce Synechocystis with n-decanal.<br />
<br />
David sent us some work he had done on luminescence assays in cyanobacteria. Following his methods we finally were able to see light emission, confirming presence of catalytically active luciferase. Thanks David!<br />
<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/7/7f/Cam.results.finalisimo.jpg" align="right" width="600"></center></html><br />
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<br /><br />
<br /><br />
<br />
<br />
<br />
<h1>Experimental Highlights</h1><br />
<br />
<br /><br />
<br /><br />
- Constructed two functional integration plasmids for <i>Synechocystis</i> PCC. 6803<br />
<br /><br />
<br /><br />
- Transformed <i>Synechocystis</i> PCC. 6803 with an optimized transformation protocol, available [https://2012.igem.org/Team:UC_Chile/Protocols#Transformation_of_Synechocystis_PCC._6803 here]<br />
<br /><br />
<br /><br />
- Verified integration of constructs<br />
<br /><br />
<br /><br />
- Confirmed expression of genes and activity of luciferase through a bioluminescence assay with exogenous substrate.<br />
<br /><br />
<br /><br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Project"><img src="https://static.igem.org/mediawiki/2012/8/80/UC_Chile-Continue_button_see_next_projects.jpg" align="right"><br />
</html><br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Results_shortTeam:UC Chile/Cyanolux/Results short2012-10-27T03:22:23Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Construction</h1><br />
<h2>Plasmids backbones</h2><br />
<br />
<br />
We built two integrative plasmid backbones for Synechocystis PCC. 6803 (see design [https://2012.igem.org/Team:UC_Chile/Cyanolux/Project_short#Wetlab_strategy here]) <br />
<br />
[[File:UC_Chile-IntSplasmidResult.jpg| 560px | right]]<br />
<br /><br />
<br /><br />
<br />
[[File:UC_Chile-IntKplasmidResult.jpg| 400px | left]]<br />
<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
Plasmids were verified by digestion and then corroborated by sequencing.<br />
<br />
<br />
<h2>Constructs for Synechocystis</h2><br />
<br />
<br />
Using our plasmid backbones as a starting point, we proceeded to build our constructs for <i>Synechocystis</i>:<br />
<br />
<br />
[[File:UC_Chile-IntKfullplasmidResult.jpg| 640px]]<br />
<br /><br />
<br /><br />
Even though we tried many times (>6) to assemble the substrate production (LuxCDEG) plasmid,we were unable to obtain a correct product. We are currently designing another strategy to build the construct...<br />
<br />
<br /><br />
<br /><br />
<br />
<h1>Characterization in Synechocystis</h1><br />
<br />
<br /><br />
<br />
<h2>Transformation</h2><br />
<br />
<br />
We used our plasmid backbones to transform Synechocystis PCC. 6803 to verify that our initial design could indeed integrate into <i>Synechocystis's</i> genome.<br />
<br />
<br />
Transformation with pSB1C3_IntK (BBa_K743015):<br />
<br />
<br />
[[File:Pta_LuxABvf.JPG| 600px| center]]<br />
<br />
<br />
Transformation with pSB1C3_IntS (BBa_K743010):<br />
<br />
<br />
[[File:Trans_IntS.JPG| 600px| center]]<br />
<br />
<br />
After two weeks, colonies became apparent in the transformation plates for both plasmid backbones while in negative controls (transformations with no DNA) there was complete absence of surviving colonies.<br />
<br />
<br />
<h2>Verification of constructs</h2><br />
<br />
<br />
We proceeded to verify the integration of the constructs in <i>Synechocystis</i> by doing multiple PCRs to amplify various parts.<br />
<br />
[[File: UC_Chile-verification.jpg]]<br />
<br />
<br />
<h2>Bioluminescence Assays</h2><br />
<br />
We proceeded to check if our <i>Synechocystis</i> strain with the Luciferase genes did indeed produce light in the prescence of exogenous substrate.<br />
<br />
<h3>Bioluminometer</h3><br />
<br />
We measured bioluminescence by adding directly the substrate to the cells and measuring light-output in a Luminometer. <br />
<br />
[[File: UC_Chile-decanalgraph.jpg]]<br />
<br />
While we could measure bioluminescence of the positive LuxBrick E.coli controls, no apparent bioluminescence was seen in our <i>Synechocystis</i> cells. This led us to think that the problem might be the size of the promoter, which if not long enough, would not be able to recruit necessary transcription factors for expression.<br />
<br />
<br />
<h3>High-sensitive camera</h3><br />
<br />
<br />
During the Latin America Jamboree, we had a chat with a couple of judges and a student from Universidad de los Andes, David Olarte, on how to induce Synechocystis with n-decanal.<br />
<br />
David sent us some work he had done on luminescence assays in cyanobacteria. Following his methods we finally were able to see light emission, confirming presence of catalytically active luciferase. Thanks David!<br />
<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/7/7f/Cam.results.finalisimo.jpg" align="right" width="600"></center></html><br />
<br />
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<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
<br />
<h1>Experimental Highlights</h1><br />
<br />
<br /><br />
<br /><br />
- Constructed two functional integration plasmids for <i>Synechocystis</i> PCC. 6803<br />
<br /><br />
<br /><br />
- Transformed <i>Synechocystis</i> PCC. 6803 with an optimized transformation protocol, available [https://2012.igem.org/Team:UC_Chile/Protocols#Transformation_of_Synechocystis_PCC._6803 here]<br />
<br /><br />
<br /><br />
- Verified integration of constructs<br />
<br /><br />
<br /><br />
- Confirmed expression of genes and activity of luciferase through a bioluminescence assay with exogenous substrate.<br />
<br /><br />
<br /><br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Project"><img src="https://static.igem.org/mediawiki/2012/8/80/UC_Chile-Continue_button_see_next_projects.jpg" align="right"><br />
</html><br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Results_shortTeam:UC Chile/Cyanolux/Results short2012-10-27T03:19:33Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Construction</h1><br />
<h2>Plasmids backbones</h2><br />
<br />
<br />
We built two integrative plasmid backbones for Synechocystis PCC. 6803 (see design [https://2012.igem.org/Team:UC_Chile/Cyanolux/Project_short#Wetlab_strategy here]) <br />
<br />
[[File:UC_Chile-IntSplasmidResult.jpg| 560px | right]]<br />
<br /><br />
<br /><br />
<br />
[[File:UC_Chile-IntKplasmidResult.jpg| 400px | left]]<br />
<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
Plasmids were verified by digestion and then corroborated by sequencing.<br />
<br />
<br />
<h2>Constructs for Synechocystis</h2><br />
<br />
<br />
Using our plasmid backbones as a starting point, we proceeded to build our constructs for <i>Synechocystis</i>:<br />
<br />
<br />
[[File:UC_Chile-IntKfullplasmidResult.jpg| 640px]]<br />
<br /><br />
<br /><br />
Even though we tried many times (>6) to assemble the substrate production (LuxCDEG) plasmid,we were unable to obtain a correct product. We are currently designing another strategy to build the construct...<br />
<br />
<br /><br />
<br /><br />
<br />
<h1>Characterization in Synechocystis</h1><br />
<br />
<br /><br />
<br />
<h2>Transformation</h2><br />
<br />
<br />
We used our plasmid backbones to transform Synechocystis PCC. 6803 to verify that our initial design could indeed integrate into <i>Synechocystis's</i> genome.<br />
<br />
<br />
Transformation with pSB1C3_IntK (BBa_K743015):<br />
<br />
<br />
[[File:Pta_LuxABvf.JPG| 600px| center]]<br />
<br />
<br />
Transformation with pSB1C3_IntS (BBa_K743010):<br />
<br />
<br />
[[File:Trans_IntS.JPG| 600px| center]]<br />
<br />
<br />
After two weeks, colonies became apparent in the transformation plates for both plasmid backbones while in negative controls (transformations with no DNA) there was complete absence of surviving colonies.<br />
<br />
<br />
<h2>Verification of constructs</h2><br />
<br />
<br />
We proceeded to verify the integration of the constructs in <i>Synechocystis</i> by doing multiple PCRs to amplify various parts.<br />
<br />
[[File: UC_Chile-verification.jpg]]<br />
<br />
<br />
<h2>Bioluminescence Assays</h2><br />
<br />
We proceeded to check if our <i>Synechocystis</i> strain with the Luciferase genes did indeed produce light in the prescence of exogenous substrate.<br />
<br />
<h3>Bioluminometer</h3><br />
<br />
We measured bioluminescence by adding directly the substrate to the cells and measuring light-output in a Luminometer. <br />
<br />
[[File: UC_Chile-decanalgraph.jpg]]<br />
<br />
While we could measure bioluminescence of the positive LuxBrick E.coli controls, no apparent bioluminescence was seen in our <i>Synechocystis</i> cells. This led us to think that the problem might be the size of the promoter, which if not long enough, would not be able to recruit necessary transcription factors for expression.<br />
<br />
<br />
<h3>High-sensitive camera</h3><br />
<br />
<br />
During the Latin America Jamboree, we had a chat with a couple of judges and a student from the Universidad de los Andes, David Olarte, on how to induce Synechocystis with n-decanal.<br />
<br />
David sent us some work he had done on luminescence assays in cyanobacteria. Following his methods we finally were able to see light emittion, confirming prescence of catalytically active luciferase.<br />
<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/7/7f/Cam.results.finalisimo.jpg" align="right" width="600"></center></html><br />
<br />
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<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
<h1>Experimental Highlights</h1><br />
<br />
<br /><br />
<br /><br />
- Constructed two functional integration plasmids for <i>Synechocystis</i> PCC. 6803<br />
<br /><br />
<br /><br />
- Transformed <i>Synechocystis</i> PCC. 6803 with an optimized transformation protocol, available [https://2012.igem.org/Team:UC_Chile/Protocols#Transformation_of_Synechocystis_PCC._6803 here]<br />
<br /><br />
<br /><br />
- Verified integration of constructs<br />
<br /><br />
<br /><br />
- Confirmed expression of genes and activity of luciferase through a bioluminescence assay with exogenous substrate.<br />
<br /><br />
<br /><br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Project"><img src="https://static.igem.org/mediawiki/2012/8/80/UC_Chile-Continue_button_see_next_projects.jpg" align="right"><br />
</html><br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Project_shortTeam:UC Chile/Cyanolux/Project short2012-10-27T03:17:04Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Main Goal:</h1><br />
<br />
Our project consists on achieving bioluminescence controlled under circadian rhythms with long-term functionality. Our aim is to produce a bioluminescent cyanobacteria which lights up during dusk hours and regenerates the substrates during the day.<br />
<br />
<h2>Rationale:</h2><br />
<br />
The importance of Biological context in Synthetic Biology has been largely underestimated. We have addressed this issue by centering our project on enhancing functionality of a previously characterized Biobrick, LuxBrick, by placing it in a context which allows new features.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/3/3f/Rationaleschemechile.jpg" align="middle" width="996"></center></html><br />
<br />
<h3>Bioluminescence</h3><br />
<br />
<!-->Bioluminescence is a enzymatic reaction which yield photons in the process, producing light.<--><br />
<br />
In 2010 the Cambridge iGEM team Biobricked the LuxBrick, a collection of genes from the Lux operon that incorporate both the Luciferase and the substrate production enzymes without regulation, allowing endogenous bioluminescence on E. coli.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Biolumilulilu.jpg" align="left" width="720"></center></html><br />
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<h3>Chassis</h3><br />
<br />
We have chosen to work with a model cyanobacteria, Synechocystis PCC. 6803 because it exhibits autotrophic metabolism and circadian rhythms. <br />
<br />
Coupling the endogenous circadian rhythms of this organism to the expression of the Lux genes will enable high-level functionality, through an automatically switching system that turns on bioluminescence only when needed.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/da/Synefeatures2.jpg" align="left" width="720"></center></html><br />
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<h2>Strategy</h2><br />
The limiting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control light emission over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Dospromotorusi.jpg" align="middle" width="850"></center></html><br />
<br />
<br />
In turn, the production of these enzymes can be specifically set to any desired time of the day by fusing their CDSs to promoters controlled by the circadian rhythm.<br />
<br />
<h3>Mathematical Modelling</h3><br />
<br />
Our model works as a “black box” in which the input takes the form of a specific hour of the day (i.e the hour on which you want your metabolite to reach maximal concentration) and the output is a couple of promoters from Synechocystis genome. <br />
It assumes that the metabolite's production is controlled by enzymes under the control of promoter 1 and it´s degradation by enzymes under promoter 2.<br />
For more details please check [https://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling here]<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/db/Blackbox.2.jpg" align="middle" width="660"></center></html><br />
<br />
<br />
<h3>Wetlab strategy</h3><br />
<br />
Having chosen the right promoters we set out to built our constructs to transform Synechocystis.<br />
As there weren´t straighforward tools to start working with in the registry (i.e characterized plasmids backbones, protocols, etc) we started from scratch.<br />
<br />
We designed two recombination plasmids backbones. One targets a gene essential for our chassis survival in the environment [https://2012.igem.org/Team:UC_Chile/Biosafety#Susceptibility_Construct (see biosafety)] and the other one a neutral site.<br />
<br />
<br />
[[File:UC_Chile-IntKstrategy.jpg | 480px | left]]<br />
<br />
[[File:CopSmutants.jpg | 480px | right]]<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br /><br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
Our strategy was to insert the LuxAB genes (luciferase) under a circadian promoter with an expression peak right after dusk, in the neutral recombination plasmid; and the LuxCDEG genes (substrate production) with the appropriate promoter in the biosafety plasmid.<br />
<br />
See [https://2012.igem.org/Team:UC_Chile/Cyanolux/Results_short#Plasmid_construction plasmids in results section]<br />
<br />
<h2>Implementation</h2><br />
<br />
Synthetic biology inspires in nature making abstractions of its principles and mechanisms. We thought this moto could be applied beyond mollecular genetics...<br />
<br />
With the relevance of context in mind, a biomimetic biolamp structure was designed that resembles the organ in which Vibrio fischeri -the bacteria from which the lux genes were biobricked- lives.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/b/b3/Biomimetic.jpg" align="middle" width="860"></center></html><br />
<br />
<br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp Full description of the biolamp device here]<br />
<br />
<br />
<div style="float:right"><br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Project See more about the whole project]<br />
</div><br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Project_shortTeam:UC Chile/Cyanolux/Project short2012-10-27T03:16:28Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Main Goal:</h1><br />
<br />
Our project consists on achieving bioluminescence controlled under circadian rhythms with long-term functionality. Our aim is to produce a bioluminescent cyanobacteria which lights up during dusk hours and regenerates the substrates during the day.<br />
<br />
<h2>Rationale:</h2><br />
<br />
The importance of Biological context in Synthetic Biology has been largely underestimated. We have addressed this issue by centering our project on enhancing functionality of a previously characterized Biobrick, LuxBrick, by placing it in a context which allows new features.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/3/3f/Rationaleschemechile.jpg" align="middle" width="996"></center></html><br />
<br />
<h3>Bioluminescence</h3><br />
<br />
<!-->Bioluminescence is a enzymatic reaction which yield photons in the process, producing light.<--><br />
<br />
In 2010 the Cambridge iGEM team Biobricked the LuxBrick, a collection of genes from the Lux operon that incorporate both the Luciferase and the substrate production enzymes without regulation, allowing endogenous bioluminescence on E. coli.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Biolumilulilu.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h3>Chassis</h3><br />
<br />
We have chosen to work with a model cyanobacteria, Synechocystis PCC. 6803 because it exhibits autotrophic metabolism and circadian rhythms. <br />
<br />
Coupling the endogenous circadian rhythms of this organism to the expression of the Lux genes will enable high-level functionality, through an automatically switching system that turns on bioluminescence only when needed.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/da/Synefeatures2.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h2>Strategy</h2><br />
The limiting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control light emission over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Dospromotorusi.jpg" align="middle" width="850"></center></html><br />
<br />
<br />
In turn, the production of these enzymes can be specifically set to any desired time of the day by fusing their CDSs to promoters controlled by the circadian rhythm.<br />
<br />
<h3>Mathematical Modelling</h3><br />
<br />
Our model works as a “black box” in which the input takes the form of a specific hour of the day (i.e the hour on which you want your metabolite to reach maximal concentration) and the output is a couple of promoters from Synechocystis genome. <br />
It assumes that the metabolite's production is controlled by enzymes under the control of promoter 1 and it´s degradation by enzymes under promoter 2.<br />
For more details please check [https://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling here]<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/db/Blackbox.2.jpg" align="middle" width="660"></center></html><br />
<br />
<br />
<h3>Wetlab strategy</h3><br />
<br />
Having chosen the right promoters we set out to built our constructs to transform Synechocystis.<br />
As there weren´t straighforward tools to start working with in the registry (i.e characterized plasmids backbones, protocols, etc) we started from scratch.<br />
<br />
We designed two recombination plasmids backbones. One targets a gene essential for our chassis survival in the environment [https://2012.igem.org/Team:UC_Chile/Biosafety#Susceptibility_Construct (see biosafety)] and the other one a neutral site.<br />
<br />
<br />
[[File:UC_Chile-IntKstrategy.jpg | 480px | left]]<br />
<br />
[[File:CopSmutants.jpg | 480px | right]]<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br /><br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
Our strategy was to insert the LuxAB genes (luciferase) under a circadian promoter with an expression peak right after dusk, in the neutral recombination plasmid; and the LuxCDEG genes (substrate production) with the appropriate promoter in the biosafety plasmid.<br />
<br />
See [https://2012.igem.org/Team:UC_Chile/Cyanolux/Results_short#Plasmid_construction plasmids in results section]<br />
<br />
<h2>Implementation</h2><br />
<br />
Synthetic biology inspires in nature making abstractions of its principles and mechanisms. We thought this moto could be applied beyond mollecular genetics...<br />
<br />
With the relevance of context in mind, a biomimetic biolamp structure was designed that resembles the organ in which Vibrio fischeri -the bacteria from which the lux genes were biobricked- lives.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/b/b3/Biomimetic.jpg" align="middle" width="860"></center></html><br />
<br />
<br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp Full description of the biolamp device here]<br />
<br />
<br />
<div style="float:right"><br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Project See more about the project]<br />
</div><br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/BiolampTeam:UC Chile/Cyanolux/Biolamp2012-10-27T03:15:14Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
== Biolamp ==<br />
<br />
We designed a stylish and translucent container to house our modified <i>Synechocystis</i>. Considering the attributes of these bacteria this will be the first self-rechargeable, programmable and eco-friendly biological lamp. It will emit light only at night, and during the day it will recharge thanks to the Photoautotrophic nature of <i>Synechocystis</i>.<br />
<br />
It consists basically in a structure made of acrylic with the form similar to a hollow torus. We chose acrylic because it is transparent and has a minimal fragility.<br />
<br />
Currently we are working with <html><a href="http://www.novaluz.cl/" target="_blank">this workshop</a></html> to evaluate the fabrication possibilities, materials and costs. We estimate that the biolamp will be finished two weeks after the wiki freeze.<br />
<br />
Besides, we are planing to implement two additional characteristics to the biolamp:<br />
<br />
1) To include reflective walls in some areas of the inside of the receptacle. This idea is motivated in the anatomical structure of <i>Euprymna scolopes</i> (see image below).<br />
<br />
2) To fabricate walls of irregular thickness with the geometry indicated in the image below. That way the biolamp will have two chambers joined by a small space. Then the receptacle should be filled to the half of its capacity. <br />
<br />
[[File:UC_Chile-Luxilla-Biolamp.jpg|right|400 px]]<br />
[[File:UC_Chile-Glumiglowi.png|500 px|left]]<br />
[[File:UC_Chile-Biolamp_interior.png|400 px|right]]<br />
<br />
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Biobricks"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Project_shortTeam:UC Chile/Cyanolux/Project short2012-10-27T03:09:22Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Main Goal:</h1><br />
<br />
Our project consists on achieving bioluminescence controlled under circadian rhythms with long-term functionality. Our aim is to produce a bioluminescent cyanobacteria which lights up during dusk hours and regenerates the substrates during the day.<br />
<br />
<h2>Rationale:</h2><br />
<br />
The importance of Biological context in Synthetic Biology has been largely underestimated. We have addressed this issue by centering our project on enhancing functionality of a previously characterized Biobrick, LuxBrick, by placing it in a context which allows new features.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/3/3f/Rationaleschemechile.jpg" align="middle" width="996"></center></html><br />
<br />
<h3>Bioluminescence</h3><br />
<br />
<!-->Bioluminescence is a enzymatic reaction which yield photons in the process, producing light.<--><br />
<br />
In 2010 the Cambridge iGEM team Biobricked the LuxBrick, a collection of genes from the Lux operon that incorporate both the Luciferase and the substrate production enzymes without regulation, allowing endogenous bioluminescence on E. coli.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Biolumilulilu.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h3>Chassis</h3><br />
<br />
We have chosen to work with a model cyanobacteria, Synechocystis PCC. 6803 because it exhibits autotrophic metabolism and circadian rhythms. <br />
<br />
Coupling the endogenous circadian rhythms of this organism to the expression of the Lux genes will enable high-level functionality, through an automatically switching system that turns on bioluminescence only when needed.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/da/Synefeatures2.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h2>Strategy</h2><br />
The limiting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control light emission over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Dospromotorusi.jpg" align="middle" width="850"></center></html><br />
<br />
<br />
In turn, the production of these enzymes can be specifically set to any desired time of the day by fusing their CDSs to promoters controlled by the circadian rhythm.<br />
<br />
<h3>Mathematical Modelling</h3><br />
<br />
Our model works as a “black box” in which the input takes the form of a specific hour of the day (i.e the hour on which you want your metabolite to reach maximal concentration) and the output is a couple of promoters from Synechocystis genome. <br />
It assumes that the metabolite's production is controlled by enzymes under the control of promoter 1 and it´s degradation by enzymes under promoter 2.<br />
For more details please check [https://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling here]<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/db/Blackbox.2.jpg" align="middle" width="660"></center></html><br />
<br />
<br />
<h3>Wetlab strategy</h3><br />
<br />
Having chosen the right promoters we set out to built our constructs to transform Synechocystis.<br />
As there weren´t straighforward tools to start working with in the registry (i.e characterized plasmids backbones, protocols, etc) we started from scratch.<br />
<br />
We designed two recombination plasmids backbones. One targets a gene essential for our chassis survival in the environment [https://2012.igem.org/Team:UC_Chile/Biosafety#Susceptibility_Construct (see biosafety)] and the other one a neutral site.<br />
<br />
<br />
[[File:UC_Chile-IntKstrategy.jpg | 480px | left]]<br />
<br />
[[File:CopSmutants.jpg | 480px | right]]<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br /><br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
Our strategy was to insert the LuxAB genes (luciferase) under a circadian promoter with an expression peak right after dusk, in the neutral recombination plasmid; and the LuxCDEG genes (substrate production) with the appropriate promoter in the biosafety plasmid.<br />
<br />
See [https://2012.igem.org/Team:UC_Chile/Cyanolux/Results_short#Plasmid_construction plasmids in results section]<br />
<br />
<h2>Implementation</h2><br />
<br />
Synthetic biology inspires in nature making abstractions of its principles and mechanisms. We thought this moto could be applied beyond mollecular genetics...<br />
<br />
With the relevance of context in mind, a biomimetic biolamp structure was designed that resembles the organ in which Vibrio fischeri -the bacteria from which the lux genes were biobricked- lives.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/b/b3/Biomimetic.jpg" align="middle" width="860"></center></html><br />
<br />
<br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp Full description of the device here]<br />
<br />
<br />
<div style="float:right"><br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Project See more about the project]<br />
</div><br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Project_shortTeam:UC Chile/Cyanolux/Project short2012-10-27T03:08:51Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Main Goal:</h1><br />
<br />
Our project consists on achieving bioluminescence controlled under circadian rhythms with long-term functionality. Our aim is to produce a bioluminescent cyanobacteria which lights up during dusk hours and regenerates the substrates during the day.<br />
<br />
<h2>Rationale:</h2><br />
<br />
The importance of Biological context in Synthetic Biology has been largely underestimated. We have addressed this issue by centering our project on enhancing functionality of a previously characterized Biobrick, LuxBrick, by placing it in a context which allows new features.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/3/3f/Rationaleschemechile.jpg" align="middle" width="996"></center></html><br />
<br />
<h3>Bioluminescence</h3><br />
<br />
<!-->Bioluminescence is a enzymatic reaction which yield photons in the process, producing light.<--><br />
<br />
In 2010 the Cambridge iGEM team Biobricked the LuxBrick, a collection of genes from the Lux operon that incorporate both the Luciferase and the substrate production enzymes without regulation, allowing endogenous bioluminescence on E. coli.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Biolumilulilu.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h3>Chassis</h3><br />
<br />
We have chosen to work with a model cyanobacteria, Synechocystis PCC. 6803 because it exhibits autotrophic metabolism and circadian rhythms. <br />
<br />
Coupling the endogenous circadian rhythms of this organism to the expression of the Lux genes will enable high-level functionality, through an automatically switching system that turns on bioluminescence only when needed.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/da/Synefeatures2.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h2>Strategy</h2><br />
The limiting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control light emission over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Dospromotorusi.jpg" align="middle" width="850"></center></html><br />
<br />
<br />
In turn, the production of these enzymes can be specifically set to any desired time of the day by fusing their CDSs to promoters controlled by the circadian rhythm.<br />
<br />
<h3>Mathematical Modelling</h3><br />
<br />
Our model works as a “black box” in which the input takes the form of a specific hour of the day (i.e the hour on which you want your metabolite to reach maximal concentration) and the output is a couple of promoters from Synechocystis genome. <br />
It assumes that the metabolite's production is controlled by enzymes under the control of promoter 1 and it´s degradation by enzymes under promoter 2.<br />
For more details please check [https://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling here]<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/db/Blackbox.2.jpg" align="middle" width="660"></center></html><br />
<br />
<br />
<h3>Wetlab strategy</h3><br />
<br />
Having chosen the right promoters we set out to built our constructs to transform Synechocystis.<br />
As there weren´t straighforward tools to start working with in the registry (i.e characterized plasmids backbones, protocols, etc) we started from scratch.<br />
<br />
We designed two recombination plasmids backbones. One targets a gene essential for our chassis survival in the environment [https://2012.igem.org/Team:UC_Chile/Biosafety#Susceptibility_Construct see biosafety] and the other one a neutral site.<br />
<br />
<br />
[[File:UC_Chile-IntKstrategy.jpg | 480px | left]]<br />
<br />
[[File:CopSmutants.jpg | 480px | right]]<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br /><br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
Our strategy was to insert the LuxAB genes (luciferase) under a circadian promoter with an expression peak right after dusk, in the neutral recombination plasmid; and the LuxCDEG genes (substrate production) with the appropriate promoter in the biosafety plasmid.<br />
<br />
See [https://2012.igem.org/Team:UC_Chile/Cyanolux/Results_short#Plasmid_construction plasmids in results section]<br />
<br />
<h2>Implementation</h2><br />
<br />
Synthetic biology inspires in nature making abstractions of its principles and mechanisms. We thought this moto could be applied beyond mollecular genetics...<br />
<br />
With the relevance of context in mind, a biomimetic biolamp structure was designed that resembles the organ in which Vibrio fischeri -the bacteria from which the lux genes were biobricked- lives.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/b/b3/Biomimetic.jpg" align="middle" width="860"></center></html><br />
<br />
<br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp Full description of the device here]<br />
<br />
<br />
<div style="float:right"><br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Project See more about the project]<br />
</div><br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Project_shortTeam:UC Chile/Cyanolux/Project short2012-10-27T03:08:18Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Main Goal:</h1><br />
<br />
Our project consists on achieving bioluminescence controlled under circadian rhythms with long-term functionality. Our aim is to produce a bioluminescent cyanobacteria which lights up during dusk hours and regenerates the substrates during the day.<br />
<br />
<h2>Rationale:</h2><br />
<br />
The importance of Biological context in Synthetic Biology has been largely underestimated. We have addressed this issue by centering our project on enhancing functionality of a previously characterized Biobrick, LuxBrick, by placing it in a context which allows new features.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/3/3f/Rationaleschemechile.jpg" align="middle" width="996"></center></html><br />
<br />
<h3>Bioluminescence</h3><br />
<br />
<!-->Bioluminescence is a enzymatic reaction which yield photons in the process, producing light.<--><br />
<br />
In 2010 the Cambridge iGEM team Biobricked the LuxBrick, a collection of genes from the Lux operon that incorporate both the Luciferase and the substrate production enzymes without regulation, allowing endogenous bioluminescence on E. coli.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Biolumilulilu.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h3>Chassis</h3><br />
<br />
We have chosen to work with a model cyanobacteria, Synechocystis PCC. 6803 because it exhibits autotrophic metabolism and circadian rhythms. <br />
<br />
Coupling the endogenous circadian rhythms of this organism to the expression of the Lux genes will enable high-level functionality, through an automatically switching system that turns on bioluminescence only when needed.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/da/Synefeatures2.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h2>Strategy</h2><br />
The limiting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control light emission over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Dospromotorusi.jpg" align="middle" width="850"></center></html><br />
<br />
<br />
In turn, the production of these enzymes can be specifically set to any desired time of the day by fusing their CDSs to promoters controlled by the circadian rhythm.<br />
<br />
<h3>Mathematical Modelling</h3><br />
<br />
Our model works as a “black box” in which the input takes the form of a specific hour of the day (i.e the hour on which you want your metabolite to reach maximal concentration) and the output is a couple of promoters from Synechocystis genome. <br />
It assumes that the metabolite's production is controlled by enzymes under the control of promoter 1 and it´s degradation by enzymes under promoter 2.<br />
For more details please check [https://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling here]<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/db/Blackbox.2.jpg" align="middle" width="660"></center></html><br />
<br />
<br />
<h3>Wetlab strategy</h3><br />
<br />
Having chosen the right promoters we set out to built our constructs to transform Synechocystis.<br />
As there weren´t straighforward tools to start working with in the registry (i.e characterized plasmids backbones, protocols, etc) we started from scratch.<br />
<br />
We designed two recombination plasmids backbones. One targets a gene essential for our chassis survival in the environment [https://2012.igem.org/Team:UC_Chile/Biosafety#Susceptibility_Construct<br />
see biosafety] and the other one a neutral site.<br />
<br />
<br />
[[File:UC_Chile-IntKstrategy.jpg | 480px | left]]<br />
<br />
[[File:CopSmutants.jpg | 480px | right]]<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br /><br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
Our strategy was to insert the LuxAB genes (luciferase) under a circadian promoter with an expression peak right after dusk, in the neutral recombination plasmid; and the LuxCDEG genes (substrate production) with the appropriate promoter in the biosafety plasmid.<br />
<br />
See [https://2012.igem.org/Team:UC_Chile/Cyanolux/Results_short#Plasmid_construction plasmids in results section]<br />
<br />
<h2>Implementation</h2><br />
<br />
Synthetic biology inspires in nature making abstractions of its principles and mechanisms. We thought this moto could be applied beyond mollecular genetics...<br />
<br />
With the relevance of context in mind, a biomimetic biolamp structure was designed that resembles the organ in which Vibrio fischeri -the bacteria from which the lux genes were biobricked- lives.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/b/b3/Biomimetic.jpg" align="middle" width="860"></center></html><br />
<br />
<br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp Full description of the device here]<br />
<br />
<br />
<div style="float:right"><br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Project See more about the project]<br />
</div><br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Project_shortTeam:UC Chile/Cyanolux/Project short2012-10-27T03:07:15Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Main Goal:</h1><br />
<br />
Our project consists on achieving bioluminescence controlled under circadian rhythms with long-term functionality. Our aim is to produce a bioluminescent cyanobacteria which lights up during dusk hours and regenerates the substrates during the day.<br />
<br />
<h2>Rationale:</h2><br />
<br />
The importance of Biological context in Synthetic Biology has been largely underestimated. We have addressed this issue by centering our project on enhancing functionality of a previously characterized Biobrick, LuxBrick, by placing it in a context which allows new features.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/3/3f/Rationaleschemechile.jpg" align="middle" width="996"></center></html><br />
<br />
<h3>Bioluminescence</h3><br />
<br />
<!-->Bioluminescence is a enzymatic reaction which yield photons in the process, producing light.<--><br />
<br />
In 2010 the Cambridge iGEM team Biobricked the LuxBrick, a collection of genes from the Lux operon that incorporate both the Luciferase and the substrate production enzymes without regulation, allowing endogenous bioluminescence on E. coli.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Biolumilulilu.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h3>Chassis</h3><br />
<br />
We have chosen to work with a model cyanobacteria, Synechocystis PCC. 6803 because it exhibits autotrophic metabolism and circadian rhythms. <br />
<br />
Coupling the endogenous circadian rhythms of this organism to the expression of the Lux genes will enable high-level functionality, through an automatically switching system that turns on bioluminescence only when needed.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/da/Synefeatures2.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h2>Strategy</h2><br />
The limiting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control light emission over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Dospromotorusi.jpg" align="middle" width="850"></center></html><br />
<br />
<br />
In turn, the production of these enzymes can be specifically set to any desired time of the day by fusing their CDSs to promoters controlled by the circadian rhythm.<br />
<br />
<h3>Mathematical Modelling</h3><br />
<br />
Our model works as a “black box” in which the input takes the form of a specific hour of the day (i.e the hour on which you want your metabolite to reach maximal concentration) and the output is a couple of promoters from Synechocystis genome. <br />
It assumes that the metabolite's production is controlled by enzymes under the control of promoter 1 and it´s degradation by enzymes under promoter 2.<br />
For more details please check [https://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling here]<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/db/Blackbox.2.jpg" align="middle" width="660"></center></html><br />
<br />
<br />
<h3>Wetlab strategy</h3><br />
<br />
Having chosen the right promoters we set out to built our constructs to transform Synechocystis.<br />
As there weren´t straighforward tools to start working with in the registry (i.e characterized plasmids backbones, protocols, etc) we started from scratch.<br />
<br />
We designed two recombination plasmids backbones. One targets a gene essential for our chassis survival in the environment ([https://2012.igem.org/Team:UC_Chile/Biosafety#Susceptibility_Construct<br />
see biosafety]) and the other one a neutral site.<br />
<br />
<br />
[[File:UC_Chile-IntKstrategy.jpg | 480px | left]]<br />
<br />
[[File:CopSmutants.jpg | 480px | right]]<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br /><br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
Our strategy was to insert the LuxAB genes (luciferase) under a circadian promoter with an expression peak right after dusk, in the neutral recombination plasmid; and the LuxCDEG genes (substrate production) with the appropriate promoter in the biosafety plasmid.<br />
<br />
See [https://2012.igem.org/Team:UC_Chile/Cyanolux/Results_short#Plasmid_construction plasmids in results section]<br />
<br />
<h2>Implementation</h2><br />
<br />
Synthetic biology inspires in nature making abstractions of its principles and mechanisms. We thought this moto could be applied beyond mollecular genetics...<br />
<br />
With the relevance of context in mind, a biomimetic biolamp structure was designed that resembles the organ in which Vibrio fischeri -the bacteria from which the lux genes were biobricked- lives.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/b/b3/Biomimetic.jpg" align="middle" width="860"></center></html><br />
<br />
<br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp Full description of the device here]<br />
<br />
<br />
<div style="float:right"><br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Project See more about the project]<br />
</div><br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Project_shortTeam:UC Chile/Cyanolux/Project short2012-10-27T03:04:31Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Main Goal:</h1><br />
<br />
Our project consists on achieving bioluminescence controlled under circadian rhythms with long-term functionality. Our aim is to produce a bioluminescent cyanobacteria which lights up during dusk hours and regenerates the substrates during the day.<br />
<br />
<h2>Rationale:</h2><br />
<br />
The importance of Biological context in Synthetic Biology has been largely underestimated. We have addressed this issue by centering our project on enhancing functionality of a previously characterized Biobrick, LuxBrick, by placing it in a context which allows new features.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/3/3f/Rationaleschemechile.jpg" align="middle" width="996"></center></html><br />
<br />
<h3>Bioluminescence</h3><br />
<br />
<!-->Bioluminescence is a enzymatic reaction which yield photons in the process, producing light.<--><br />
<br />
In 2010 the Cambridge iGEM team Biobricked the LuxBrick, a collection of genes from the Lux operon that incorporate both the Luciferase and the substrate production enzymes without regulation, allowing endogenous bioluminescence on E. coli.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Biolumilulilu.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h3>Chassis</h3><br />
<br />
We have chosen to work with a model cyanobacteria, Synechocystis PCC. 6803 because it exhibits autotrophic metabolism and circadian rhythms. <br />
<br />
Coupling the endogenous circadian rhythms of this organism to the expression of the Lux genes will enable high-level functionality, through an automatically switching system that turns on bioluminescence only when needed.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/da/Synefeatures2.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h2>Strategy</h2><br />
The limiting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control light emission over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Dospromotorusi.jpg" align="middle" width="850"></center></html><br />
<br />
<br />
In turn, the production of these enzymes can be specifically set to any desired time of the day by fusing their CDSs to promoters controlled by the circadian rhythm.<br />
<br />
<h3>Mathematical Modelling</h3><br />
<br />
Our model works as a “black box” in which the input takes the form of a specific hour of the day (i.e the hour on which you want your metabolite to reach maximal concentration) and the output is a couple of promoters from Synechocystis genome. <br />
It assumes that the metabolite's production is controlled by enzymes under the control of promoter 1 and it´s degradation by enzymes under promoter 2.<br />
For more details please check [https://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling here]<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/db/Blackbox.2.jpg" align="middle" width="660"></center></html><br />
<br />
<br />
<h3>Wetlab strategy</h3><br />
<br />
Having chosen the right promoters we set out to built our constructs to transform Synechocystis.<br />
As there weren´t straighforward tools to start working with in the registry (i.e characterized plasmids backbones, protocols, etc) we started from scratch.<br />
<br />
We designed two recombination plasmids backbones. One targets a gene essential for our chassis survival in the environment (link:see biosafety) and the other one a neutral site.<br />
<br />
[[File:UC_Chile-IntKstrategy.jpg | 480px | left]]<br />
<br />
[[File:CopSmutants.jpg | 480px | right]]<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br /><br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
Our strategy was to insert the LuxAB genes (luciferase) under a circadian promoter with an expression peak right after dusk, in the neutral recombination plasmid; and the LuxCDEG genes (substrate production) with the appropriate promoter in the biosafety plasmid.<br />
<br />
See [https://2012.igem.org/Team:UC_Chile/Cyanolux/Results_short#Plasmid_construction plasmids in results section]<br />
<br />
<h2>Implementation</h2><br />
<br />
Synthetic biology inspires in nature making abstractions of its principles and mechanisms. We thought this moto could be applied beyond mollecular genetics...<br />
<br />
With the relevance of context in mind, a biomimetic biolamp structure was designed that resembles the organ in which Vibrio fischeri -the bacteria from which the lux genes were biobricked- lives.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/b/b3/Biomimetic.jpg" align="middle" width="860"></center></html><br />
<br />
<br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp Full description of the device here]<br />
<br />
<br />
<div style="float:right"><br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Project See more about the project]<br />
</div><br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyanolux/Project_shortTeam:UC Chile/Cyanolux/Project short2012-10-27T03:01:57Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Main Goal:</h1><br />
<br />
Our project consists on achieving bioluminescence controlled under circadian rhythms with long-term functionality. Our aim is to produce a bioluminescent cyanobacteria which lights up during dusk hours and regenerates the substrates during the day.<br />
<br />
<h2>Rationale:</h2><br />
<br />
The importance of Biological context in Synthetic Biology has been largely underestimated. We have addressed this issue by centering our project on enhancing functionality of a previously characterized Biobrick, LuxBrick, by placing it in a context which allows new features.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/3/3f/Rationaleschemechile.jpg" align="middle" width="996"></center></html><br />
<br />
<h3>Bioluminescence</h3><br />
<br />
<!-->Bioluminescence is a enzymatic reaction which yield photons in the process, producing light.<--><br />
<br />
In 2010 the Cambridge iGEM team Biobricked the LuxBrick, a collection of genes from the Lux operon that incorporate both the Luciferase and the substrate production enzymes without regulation, allowing endogenous bioluminescence on E. coli.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Biolumilulilu.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h3>Chassis</h3><br />
<br />
We have chosen to work with a model cyanobacteria, Synechocystis PCC. 6803 because it exhibits autotrophic metabolism and circadian rhythms. <br />
<br />
Coupling the endogenous circadian rhythms of this organism to the expression of the Lux genes will enable high-level functionality, through an automatically switching system that turns on bioluminescence only when needed.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/da/Synefeatures2.jpg" align="left" width="720"></center></html><br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h2>Strategy</h2><br />
The limiting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control light emission over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Dospromotorusi.jpg" align="middle" width="850"></center></html><br />
<br />
<br />
In turn, the production of these enzymes can be specifically set to any desired time of the day by fusing their CDSs to promoters controlled by the circadian rhythm.<br />
<br />
<h3>Mathematical Modelling</h3><br />
<br />
Our model works as a “black box” in which the input takes the form of a specific hour of the day (i.e the hour on which you want your metabolite to reach maximal concentration) and the output is a couple of promoters from Synechocystis genome. <br />
It assumes that the metabolite's production is controlled by enzymes under the control of promoter 1 and it´s degradation by enzymes under promoter 2.<br />
For more details please check [2012.igem.org/Team:UC_Chile/Cyanolux/Modelling here]<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/d/db/Blackbox.2.jpg" align="middle" width="660"></center></html><br />
<br />
<br />
<h3>Wetlab strategy</h3><br />
<br />
Having chosen the right promoters we set out to built our constructs to transform Synechocystis.<br />
As there weren´t straighforward tools to start working with in the registry (i.e characterized plasmids backbones, protocols, etc) we started from scratch.<br />
<br />
We designed two recombination plasmids backbones. One targets a gene essential for our chassis survival in the environment (link:see biosafety) and the other one a neutral site.<br />
<br />
[[File:UC_Chile-IntKstrategy.jpg | 480px | left]]<br />
<br />
[[File:CopSmutants.jpg | 480px | right]]<br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br /><br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br /><br />
<br />
Our strategy was to insert the LuxAB genes (luciferase) under a circadian promoter with an expression peak right after dusk, in the neutral recombination plasmid; and the LuxCDEG genes (substrate production) with the appropriate promoter in the biosafety plasmid.<br />
<br />
See [https://2012.igem.org/Team:UC_Chile/Cyanolux/Results_short#Plasmid_construction plasmids in results section]<br />
<br />
<h2>Implementation</h2><br />
<br />
Synthetic biology inspires in nature making abstractions of its principles and mechanisms. We thought this moto could be applied beyond mollecular genetics...<br />
<br />
With the relevance of context in mind, a biomimetic biolamp structure was designed that resembles the organ in which Vibrio fischeri -the bacteria from which the lux genes were biobricked- lives.<br />
<html><center><img src="https://static.igem.org/mediawiki/2012/b/b3/Biomimetic.jpg" align="middle" width="860"></center></html><br />
<br />
<br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp Full description of the device here]<br />
<br />
<br />
<div style="float:right"><br />
[https://2012.igem.org/Team:UC_Chile/Cyanolux/Project See more about the project]<br />
</div><br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:50:51Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<font color="white">We would like to start by saying that many people helped throughout this project, many of whom are not mentioned in the Wiki (but we had to at the end of the day for political reasons). Any attempt of acknowledging them (them=other team members) falls short of reality...</font><br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
==Team Members==<br />
<br />
<h2>Beerdragons</h2><br />
<br />
<br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br />
<br />
<h2>Other team members</h2><br />
<br />
<br />
[[File:Seba.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Seba]]<br />
<h1> Sebastián Espinoza </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Emilia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Emi]]<br />
<h1> Emilia Díaz </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Ulises.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Ulises]]<br />
<h1> Ulises Mayol </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bryon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Bryon]]<br />
<h1> Bryon Silva </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Claudia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Claudia]]<br />
<h1> Claudia Stuckrath </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:50:39Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<font color="white">We would like to start by saying that many people helped throughout this project, many of whom are not mentioned in the Wiki (but we had to at the end of the day for political reasons). Any attempt of acknowledging them (them=other team members) falls short of reality...</font><br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
==Team Members==<br />
<br />
<h2>Beerdragons</h2><br />
<br />
<br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br />
<br />
<h2>Other team members</h2><br />
<br />
<br />
[[File:Seba.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Seba]]<br />
<h1> Sebastián Espinoza </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Emilia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Emi]]<br />
<h1> Emilia Díaz </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Ulises.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Ulises]]<br />
<h1> Ulises Mayol </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bryon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Bryon]]<br />
<h1> Bryon Silva </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Claudia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Claudia]]<br />
<h1> Claudia Stuckrath </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:49:32Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<font color="white">We would like to start by saying that many people helped throughout this project, many of whom are not mentioned in the Wiki (but we had to at the end of the day for political reasons). Any attempt of acknowledging them (them=team members) falls short of reality...</font><br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
==Team Members==<br />
<br />
<h2>Beerdragons</h2><br />
<br />
<br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br />
<br />
<h2>Other team members</h2><br />
<br />
<br />
[[File:Seba.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Seba]]<br />
<h1> Sebastián Espinoza </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Emilia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Emi]]<br />
<h1> Emilia Díaz </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Ulises.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Ulises]]<br />
<h1> Ulises Mayol </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bryon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Bryon]]<br />
<h1> Bryon Silva </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Claudia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Claudia]]<br />
<h1> Claudia Stuckrath </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:47:48Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<font color="white">We would like to start by saying that many people helped throughout this project, many of whom are not mentioned in the Wiki. Any attempt of acknowledging them (like other team members) falls short of reality...</font><br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
==Team Members==<br />
<br />
<h2>Beerdragons</h2><br />
<br />
<br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br />
<br />
<h2>Other team members</h2><br />
<br />
<br />
[[File:Seba.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Seba]]<br />
<h1> Sebastián Espinoza </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Emilia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Emi]]<br />
<h1> Emilia Díaz </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Ulises.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Ulises]]<br />
<h1> Ulises Mayol </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bryon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Bryon]]<br />
<h1> Bryon Silva </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Claudia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Claudia]]<br />
<h1> Claudia Stuckrath </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:41:03Z<p>Csvidal: /* Principal Advisor */</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
==Team Members==<br />
<br />
<h2>Beerdragons</h2><br />
<br />
<br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br />
<br />
<h2>Other team members</h2><br />
<br />
<br />
[[File:Seba.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Seba]]<br />
<h1> Sebastián Espinoza </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Emilia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Emi]]<br />
<h1> Emilia Díaz </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Ulises.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Ulises]]<br />
<h1> Ulises Mayol </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bryon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Bryon]]<br />
<h1> Bryon Silva </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Claudia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Claudia]]<br />
<h1> Claudia Stuckrath </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:40:34Z<p>Csvidal: /* Team Members */</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
==Team Members==<br />
<br />
<h2>Beerdragons</h2><br />
<br />
<br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br />
<br />
<h2>Other team members</h2><br />
<br />
<br />
[[File:Seba.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Seba]]<br />
<h1> Sebastián Espinoza </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Emilia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Emi]]<br />
<h1> Emilia Díaz </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Ulises.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Ulises]]<br />
<h1> Ulises Mayol </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bryon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Bryon]]<br />
<h1> Bryon Silva </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Claudia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Claudia]]<br />
<h1> Claudia Stuckrath </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:39:48Z<p>Csvidal: /* Team Members (AKA: Beerdragons) */</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
==Team Members==<br />
<br><br><br><br />
<br />
<h2>Beerdragons</h2><br />
<br />
<br><br><br />
<br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br><br><br />
<br />
<h2>Other team members</h2><br />
<br />
<br><br><br><br />
<br />
<br />
[[File:Seba.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Seba]]<br />
<h1> Sebastián Espinoza </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Emilia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Emi]]<br />
<h1> Emilia Díaz </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Ulises.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Ulises]]<br />
<h1> Ulises Mayol </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bryon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Bryon]]<br />
<h1> Bryon Silva </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Claudia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Claudia]]<br />
<h1> Claudia Stuckrath </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:37:58Z<p>Csvidal: /* Team Members (AKA: Beerdragons) */</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
==Team Members (AKA: Beerdragons)==<br />
<br><br><br><br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Seba.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Seba]]<br />
<h1> Sebastián Espinoza </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Emilia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Emi]]<br />
<h1> Emilia Díaz </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Ulises.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Ulises]]<br />
<h1> Ulises Mayol </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bryon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Bryon]]<br />
<h1> Bryon Silva </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Claudia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Claudia]]<br />
<h1> Claudia Stuckrath </h1><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:31:31Z<p>Csvidal: /* Team Members (AKA: Beerdragons) */</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
==Team Members (AKA: Beerdragons)==<br />
<br><br><br><br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Seba.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Seba]]<br />
<h1> Sebastián Espinoza </h1><br />
Real name: Sebastián Espinoza<br><br />
Occupation: 5th year student, biochemistry<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Emilia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Emi]]<br />
<h1> Emilia Díaz </h1><br />
Real name: Emilia Díaz<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: protein modelling in Bactomithril project<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Ulises.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Ulises]]<br />
<h1> Ulises Mayol </h1><br />
Real name: Ulises Mayol<br><br />
Occupation: 4th year student, pharmaceutical chemistry<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bryon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Bryon]]<br />
<h1> Bryon Silva </h1><br />
Real name: Bryon Silva<br><br />
Occupation: 5th year student, biochemistry<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Claudia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Claudia]]<br />
<h1> Claudia Stuckrath </h1><br />
Real name: Claudia Stuckrath <br><br />
Occupation: M.Sc in structural engineering<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:30:29Z<p>Csvidal: /* Team Members (AKA: Beerdragons) */</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
==Team Members (AKA: Beerdragons)==<br />
<br><br><br><br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Seba.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Seba]]<br />
<h1> Sebastián Espinoza </h1><br />
Real name: Sebastián Espinoza<br><br />
Occupation: 5th year student, biochemistry<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Emilia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Emi]]<br />
<h1> Emilia Díaz </h1><br />
Real name: Emilia Díaz<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: protein modelling in Bactomithril project<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Ulises.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Ulises]]<br />
<h1> Ulises Mayol </h1><br />
Real name: Ulises Mayol<br><br />
Occupation: 4th year student, pharmaceutical chemistry<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bryon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Bryon]]<br />
<h1> Bryon Silva </h1><br />
Real name: Bryon Silva<br><br />
Occupation: 5th year student, biochemistry<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Claudia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Claudia]]<br />
<h1> Claudia Stuckrath </h1><br />
Real name: Claudia Stuckrath <br><br />
Occupation: M.Sc in structural engineering<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:28:44Z<p>Csvidal: /* Team Members (AKA: Beerdragons) */</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
==Team Members (AKA: Beerdragons)==<br />
<br><br><br><br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Seba.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Seba]]<br />
<h1> Sebastián Espinoza </h1><br />
Real name: Sebastián Espinoza<br><br />
Occupation: 5th year student, biochemistry<br><br />
<br><br><br><br><br />
<br />
<br />
[[File:Emilia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Emi]]<br />
<h1> Emilia Díaz </h1><br />
Real name: Emilia Díaz<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: protein modelling in Bactomithril project<br><br />
<br />
<br><br><br><br><br><br />
<br />
[[File:Ulises.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Ulises]]<br />
<h1> Ulises Mayol </h1><br />
Real name: Ulises Mayol<br><br />
Occupation: 4th year student, pharmaceutical chemistry<br><br />
<br />
<br><br><br><br><br><br><br><br />
<br />
[[File:Bryon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Bryon]]<br />
<h1> Bryon Silva </h1><br />
Real name: Bryon Silva<br><br />
Occupation: 5th year student, biochemistry<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Claudia.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Claudia]]<br />
<h1> Claudia Stuckrath </h1><br />
Real name: Claudia Stuckrath <br><br />
Occupation: M.Sc in structural engineering<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:21:37Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
==Team Members (AKA: Beerdragons)==<br />
<br><br><br><br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br><br><br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:12:46Z<p>Csvidal: /* Acknowledgements */</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
We would like to start by saying that many people helped throughout this project, many of whom are not mentioned in the Wiki. Any attempt of acknowledging them falls short of reality... <br />
<br />
==Team Members (AKA: Beerdragons)==<br />
<br><br><br><br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br><br><br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to express our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:12:11Z<p>Csvidal: /* Acknowledgements */</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
We would like to start by saying that many people helped throughout this project, many of whom are not mentioned in the Wiki. Any attempt of acknowledging them falls short of reality... <br />
<br />
==Team Members (AKA: Beerdragons)==<br />
<br><br><br><br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br><br><br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
And finally, we would like to give our most sincere thank you to Clara Espínola (Clarita) for all the moral support, kind words and for putting up with us all this time. THANK YOU!!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Team/MembersTeam:UC Chile/Team/Members2012-10-27T02:06:17Z<p>Csvidal: /* Team Members (AKA: Beerdragons) */</p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
We would like to start by saying that many people helped throughout this project, many of whom are not mentioned in the Wiki. Any attempt of acknowledging them falls short of reality... <br />
<br />
==Team Members (AKA: Beerdragons)==<br />
<br><br><br><br />
[[File:albandw_uc_chile.jpg|left|160px]]<br />
<h1> Al </h1><br />
Real name: Al<br><br />
AKA: Al, The Bloodthirsty<br><br />
Occupation: Team's pet<br><br />
Contributions: Lifts our spirits by telling us everything will be ok<br><br />
Likes: To stare pretending he looks just like a bunch of micropipettes.<br><br />
Freak fact: had a bioluminescent orgy with a group of TOP10 cells. <br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:UC_Chile-Isaac.jpg|left|160px|link=https://static.igem.org/mediawiki/2012/a/aa/UC_Chile-Isaac.jpg]]<br />
<h1> Isaac Núñez </h1><br />
Real name: Isaac Núñez<br><br />
AKA: Aisaac, Prosimous, Sir Isaac N.<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician, brutal mathematical modelling<br><br />
Likes: Death metal, Chinesse noodles, martial arts<br><br />
Freak fact: Has 5 more siblings, can stay awake for 3 straight days, goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Carla.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Carla]]<br />
<h1> Carla Vidal </h1><br />
Real name: Unknown<br><br />
AKA: Charlene, Charlisse, Charlissette, Charlyon, Charlyonisette<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: Made all our scribblings make sense (somehow), content editing, english correction, arduino, presentation speaker<br><br />
Likes: Beer, barbecues, rain, really hot meat (lava hot), wearing evening gowns<br><br />
Freak fact: Missed her scholarship award ceremony on purpose, likes studying at the airport; has no fingerprints<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Simon.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Simon]]<br />
<h1> Simón Álamos </h1><br />
Real name: J. Simón Álamos<br><br />
AKA: THE agricultural scientist, Mr. Simon, igemito alpha, cookie monster.<br><br />
Occupation: 3rd year student, agricultural sciences<br><br />
Contributions: doing lost of digestions and ligations, in charge of the hispter style of the team.<br><br />
Likes: mannerism, chocolate ice cream at Emporio La Rosa, mate.<br><br />
Freak fact: attended art school for two years before switching to agricultural sciences. Gets his hair cut every month. Collects plastic dinosaurs<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Tamara.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Tama]]<br />
<h1> Tamara Matute </h1><br />
Real name: Tamara Matute<br><br />
AKA: Tama<br><br />
Occupation: 4th year student, bioengineering<br><br />
Contributions: Wet lab magician (made things work when they wouldn't for months), structure and organization at professional level, new application ideas for biosafety, did the most horrible maratonic experiments (i.e. 114 dif PCRs, mRFP1 vs sfGFP)<br><br />
Likes: Death metal, environmental protection, Chinese noodles<br><br />
Freak fact: Goes lab-camping<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Max.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Max]]<br />
<h1> Max Felis </h1><br />
Real name: Maximiliano Felis<br><br />
AKA: happy igemito<br><br />
Occupation: 3rd year student, bioengineering<br><br />
Contributions: most of the bactomithril project, videos, images, logo, biolamp design<br><br />
Likes: video editing, image editing, wiki editing, editing.<br><br />
Freak fact: frustrated violinist, attended music school at Viena for one year.<br><br />
<br><br><br><br><br><br />
<br />
<br />
[[File:Bernardo.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Pollak]]<br />
<h1> Bernardo Pollak </h1><br />
Real name: Bernardo Pollak<br><br />
AKA: Pollako, igemon, igemuco<br><br />
Occupation: Supposedly writing thesis. Biochemist.<br><br />
Contributions: recruited all the team. Does multitasking. Running wet lab. <br><br />
Likes: singing aloud, led lights, skating/surfing, horrible fluorescent color hoodies.<br><br />
Freak fact: found a new bioluminiscent bug in the south of Chile.... but suspiciously he could never take a reproducible sample of it. Does this gulash recipe thing no one at the lab has dared to eat.<br><br />
<br><br><br><br><br><br />
<br />
== Principal Advisor ==<br />
<br />
<br />
<br />
[[File:UC_Chile-160px-Rodrigo2.jpg|left|160px]]<br />
<h1> Rodrigo Gutiérrez </h1><br />
Won HHMI early scientist award this year.<br><br />
Published in Science this year.<br><br />
Likes to play the drums.<br><br />
Is always a step ahead.<br><br />
The myth says he did about 2000 clonings during his Ph.D. work.<br><br />
<br />
<br><br><br><br><br><br />
<br />
== Cyanobacterial Advisor ==<br />
<br />
<br />
[[File:UC_Chile-160px-MonicaV.jpg|left|160px]]<br />
<h1>Mónica Vásquez</h1><br />
Loves cyanobacteria<br><br />
Has been seen doing wetlab even though she is a PI :)<br><br />
Knows curious facts about Saxitoxin<br><br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
== International Advisor ==<br />
<br />
[[File:federicibandw_uc_chile.jpg|left|160px]]<br />
<h1>Fernán Federici</h1><br />
"The Argentinian" <br><br />
Told us he worked at Cambridge. Although we've never seen him there.<br><br />
We send him desperate e-mails when things don't work (e.g. Gibson assembly).<br />
<br><br><br><br><br><br><br />
<br />
==Advisors ==<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
[[File: UC_Chile-Alejandro_Montenegro.jpg|left|160px]]<br />
<h1> Alejandro Montenegro </h1><br />
Biological sciences doctorate student<br><br />
Likes to eat pepperoni pizza<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC-Chile-Roberto_Munita.jpg|left|160px]]<br />
<h1> Roberto Munita </h1><br />
Biological sciences doctorate student<br><br />
Knows EVERYTHING about PCR<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:UC_Chile-Felipe_Andrés_Muñoz_Guzmán.jpg|left|160px]]<br />
<h1> Felipe Muñoz </h1><br />
Biological sciences doctorate student<br><br />
Wears white to go to weddings<br><br />
<br />
<br />
<br><br><br><br><br><br />
<br />
<br />
<br />
[[File:Dinka.jpg|left|160px]]<br />
<h1> Dinka </h1><br />
Biological sciences doctorate student<br><br />
Lady cyano. Helped us with Synechocystis<br><br />
<br />
<br />
<br />
<br><br><br><br><br><br><br />
<br />
[[File:danirestovic_uc_chile.jpg|left|160px]]<br />
<h1> Daniela Restovic </h1><br />
Psychology undergraduate student<br><br />
Prevents us from going insane<br><br />
<br><br><br><br><br><br><br><br />
<br />
<br />
<br />
[[File:juanovenegas_uc_chile.jpg|left|160px]]<br />
<h1> Juano Venegas </h1><br />
Electrical engineer undergraduate student<br><br />
Microcontroller and electronics backup<br><br />
<br><br><br><br><br><br><br><br />
<br />
==Collaborator==<br />
<br />
[[File:Rolando.jpg|left|160px|link=https://2012.igem.org/Team:UC_Chile/Team/Members/Rolando]]<br />
<h1> Rolando Moraga </h1><br />
Architecture Graduate Student<br />
<br><br><br><br><br><br><br><br><br />
<br />
==Acknowledgements ==<br />
<br />
We would like to thank the following professors for their feedback to our project: Francisco Melo (Bioinformatics), Eduardo Agosín (Metabolic Engineering), Loreto Valenzuela (Biomaterials) and Ignacio Vargas (Environmental Biotechnology). We would also like to give special thanks to Mónica Vásquez and her lab (Biological Sciences) for providing us with our beloved Synechocystis cells and advice!<br />
<br />
<br />
--------------------------------------------------------------------------------------------------------------------<br />
<br />
It is impossible to express the amount of gratitute that really all the people that were involved in the realization of the project deserve. Your counsel, moral support, financing, help in many different ways were fundamental for the realization of the project... Eternal thanks.<br />
<br />
<br />
<html><br />
<a href="https://2012.igem.org/Team:UC_Chile/Team/PSB_Lab"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Community_outreachTeam:UC Chile/Community outreach2012-10-27T01:51:01Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Synthetic Sociology</h1><br />
<html><img src = "https://static.igem.org/mediawiki/2012/a/ad/SYNSOC.2.jpg" width= "970" align="center" style ="margin:15px"></html><br />
Being the first iGEM team in Chile and also part of the only group of students that participated in the first SynBio course held in our country, we faced the challenge of spreading the word. As evangelists, we planned to do with the Chilean society something analog to what we did in our project, placing our desired outputs (getting people into SynBio) downstream the endogenous machinery (schools, fairs, faculties, etc): Welcome to Synthetic Sociology.<br />
<br />
<h2>Early phase constructs: Penta UC Kids</h2><br />
<br />
<html><img src = "https://static.igem.org/mediawiki/2012/4/44/Pentauc1_uc_chile.jpg" width= "300" align="right" style ="margin:15px"></html><br />
The Talents Development and Study Program at Catholic University (PENTA UC for its initials in spanish) aims to educate and form creative and proactive kids and to reinforce their passion for knowledge. At the same time the PENTA program helps to enrich their personal and social maturation process through the development of confidence in themselves and their capacities, together with an ethical and socially responsible attitude.<br />
<br />
Through a curriculum of multiple disciplines and practical workshops lead by UC professors and students the program gives kids from socially disadvantaged contexts the opportunity to develop their potential.<br />
<br />
One of the courses, called “What if Dr. Frankenstein lived today?” is designed to introduce 13 year old kids to the practical and theoretical fundamentals of genetic engineering while addressing ethical questions in order to understand how it is possible to work with genes and use them for the benefit of mankind.<br />
<br />
This course happened to be guided by Ariel Herrera, a graduate student who is pursuing his Ph.D in the lab next to ours. We agreed that it would be great to explain the Central Dogma of molecular biology through a practical demonstration with recombinant E.coli colonies expressing fluorescent proteins and pigments.<br />
<br />
After a brief and interesting introduction by B. Pollak about plasmids, cloning and colony isolation, each of us went on to show a group of 5 kids the picking and striking of bacterial colonies under sterile and safe conditions.<br />
<br />
<html><img src = "https://static.igem.org/mediawiki/2012/d/d2/Pentauc2_uc_chile.jpg" width= "300" align="left" style ="margin:15px"></html>While explaining in simple terms how we obtained purple, green and red bacteria with Violacein synthesis pathway, GFP and RFP genes respectively, the young fellows were given freedom to “paint” with these cells in LB plates (check the results below!)<br />
<br />
After that, we showed them how to visualize the colonies under UV light.<br />
Finally, they were able to identify the differences between green autotrophic bacteria (our beloved Synechocystis PCC6803) and a heterotrophic one (Top10 E.coli) and how that is related to the growing conditions and media.<br />
GFP recombinant chimaeric-corpse engineered H. sapiens<br />
As an iGEM team we felt the experience very important to us because we could share our passion for science with kids that do not have many opportunities to enjoy molecular biology. Also, we could finally leave our tasks for a while and see the reality that surrounds us. This is an eye opener and definitely will help us keep things in perspective. We are extremely happy and grateful for contributing at least for just a few hours to the formation of the future scientists of our country.<br />
<br />
<br />
<h2>Exponential phase constructs</h2> <br />
<br />
<h3>INGENIA fair</h3><br />
<br />
The Faculty of Engineering of our university throws this cool innovation + entrepreneurship fair every year for the last two years. No wait, just the last year. And this one. Okay, so it’s not tradition yet. But it will be. Trust me on this one.<br />
This fair, called INGENIA (a witty pun with Ingeniar, wich would be the verb form of engineering in Spanish) brings some really amazing guests to our yard (huh), including Google, Un techo para chile, Start-up Chile, our Deen and almost every teacher of the faculty who had something amazing to talk about.<br />
And us.<br />
<br />
<html><img src = "https://static.igem.org/mediawiki/2012/4/44/UC_Chile-Ingenia1.png" width= "600" align="right" style ="margin:15px"></html><br />
<br />
I talked to the people in our alumni center, and they were thrilled about the idea of having us there. You see, biotechnology and bioengineering in general don’t get much of a spotlight in our faculty. Most engineers hate biology to their guts, and chemistry kind of makes them barf. I think it’s the lab alcohol, reminding them what they did last summer. And well, every summer.<br />
Back to my point.<br />
When we got the chance of having a booth and a round table for talking about synthetic biology, we couldn’t be happier –and prouder! Making this study-machines (or party-machines, it depends) interested in something other than their specialization field is our own little mission impossible. So when we (I say we, but it was actually our own architect, Rolando, who had to design and design for hours while I sent him about a hundred of versions of the text) decided to make the most amazing posters and handouts, so that at least our stand could be a little flashy. I mean, guys. We’re competing with investigation teams that bring food and wine and beer to their stands. <br />
We had pipettes. I sincerely thought we were screwed.<br />
<br />
These beauties here are our handouts and poster, respectively. Pretty, huh?<br />
<br />
[[File:UC-Chile-Ingenia2.png|left|375 px]][[File:UC_Chile-Ingenia3.png|right|375 px]][[File:UC_Chile-Ingenia4.png|200 px]]<br />
<br />
<br />
<br />
Ok, so we had amazing material. That alone should be enough to make our little booth pretty amazing, right?<br />
Yeah. Not so much.<br />
We also needed PEOPLE.<br />
By 9:49 I was definitely panicking. I had talked to the amazing total of ONE person, and only because he was offering organic chips from his stand. Damn the people who can bring food to their booth. It’s not fair. We could offer bread with luminescent fungus if we wanted okay so just leave us alone. <br />
Anyway. The booth was empty and my nerves were definitely on the edge, but everything changed when the fire nation attacked the 9:50 recess came.<br />
You can always see people flooding out of their classrooms at that exact hour, but this time it was different. We were right in the middle of their trajectory. So they HAD to stop by. And luckily, they did!<br />
We were there for about six hours. We had a little list for people who wanted to receive information about the competition and our projects. Bets came and went through the team members, and the highest bidder said there was no way we could get past twenty people (our faculty is not that big, mind you).<br />
WE HAD 32. 32! AND THEY WERE ALL REAL PEOPLE, NO ANNITA BATHS OR ANYTHING OF SORTS ON THE LIST, I CHECKED.<br />
IT WAS AWESOME.<br />
AWESOME, I SAY.<br />
<br />
<br />
[[File:UC_Chile-Ingenia5.png|235 px]]<br />
[[File:UC_Chile-Ingenia6.png|235 px]]<br />
[[File:UC_Chile-Ingenia7.png|235 px]]<br />
[[File:UC_Chile-Ingenia8.png|235 px]]<br />
<br />
<br />
<br />
[[File:UC_Chile-Ingenia9.png|left|200 px]]<br />
<br />
Most of the people we talked to were engineers specializing in biotechnology, though we did get others interested in math, computer science and mechanical engineering. If they really make it to next year’s team I highly advise you to watch out for them. Awesome interdisciplinary team approaching!<br />
Some of them were really interested in going to the lab to see the results of the projects we talked so much about. If you can do that in your school too, it’s a sure way to interest indecisive people. After all, je pense donc je suis is much more like je vois donc il est, right? <br />
Look at this! We even had the second fullest booth of the fair at some point of the afternoon! We couldn’t beat Google. They had google pencils. I got like five. They were awesome.<br />
It was an amazing day, really.<br />
The next day, we had a round table session to discuss synthetic biology and its influence in real life situations, mostly applied to our life as students. We were filmed and all, so if you’re interested you can watch the footage here: http://twitcam.livestream.com/9pslw<br />
If you and your team have the chance to participate in a similar event, go for it! It’s a great way to get students to know about your projects, iGEM, and synthetic biology in general. Remember to have some nice handouts and a flashy poster. That, or get there dressed up as bacteria, I don’t know! Whatever floats your boat… and helps you get new recruits!<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h3>CONEIB (National reunion of Biotechnology Students)</h3><br />
<br />
<br />
We presented our project at the annual biotechnology students reunion in Antogasta, Chile from October 17 -19. All the people who attended loved our project and wanted to start their own iGEM teams at their universities.<br />
<br />
<br />
<html><br />
Here is<a href="http://www.congresobiotecnologia.cl/" target="_blank"> the official web page of the event!</a></html> (in Spanish).<br />
<br />
<h2>Maturity phase constructs</h2><br />
<br />
<h3> SynBio Workshop</h3><br />
<html><img src = "https://static.igem.org/mediawiki/2012/5/50/Workshop.jpg" width= "650" align="left" style ="margin:15px"><br />
<html><br />
<br />
<a href="https://2012.igem.org/Team:UC_Chile/Workshop"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
Check the next link for [https://2012.igem.org/Team:UC_Chile/Workshop more Workshop details!]<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Community_outreachTeam:UC Chile/Community outreach2012-10-27T01:50:28Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Synthetic Sociology</h1><br />
<html><img src = "https://static.igem.org/mediawiki/2012/a/ad/SYNSOC.2.jpg" width= "970" align="center" style ="margin:15px"></html><br />
Being the first iGEM team in Chile and also part of the only group of students that participated in the first SynBio course held in our country, we faced the challenge of spreading the word. As evangelists, we planned to do with the Chilean society something analog to what we did in our project, placing our desired outputs (getting people into SynBio) downstream the endogenous machinery (schools, fairs, faculties, etc): Welcome to Synthetic Sociology.<br />
<br />
<h2>Early phase constructs: Penta UC Kids</h2><br />
<br />
<html><img src = "https://static.igem.org/mediawiki/2012/4/44/Pentauc1_uc_chile.jpg" width= "300" align="right" style ="margin:15px"></html><br />
The Talents Development and Study Program at Catholic University (PENTA UC for its initials in spanish) aims to educate and form creative and proactive kids and to reinforce their passion for knowledge. At the same time the PENTA program helps to enrich their personal and social maturation process through the development of confidence in themselves and their capacities, together with an ethical and socially responsible attitude.<br />
<br />
Through a curriculum of multiple disciplines and practical workshops lead by UC professors and students the program gives kids from socially disadvantaged contexts the opportunity to develop their potential.<br />
<br />
One of the courses, called “What if Dr. Frankenstein lived today?” is designed to introduce 13 year old kids to the practical and theoretical fundamentals of genetic engineering while addressing ethical questions in order to understand how it is possible to work with genes and use them for the benefit of mankind.<br />
<br />
This course happened to be guided by Ariel Herrera, a graduate student who is pursuing his Ph.D in the lab next to ours. We agreed that it would be great to explain the Central Dogma of molecular biology through a practical demonstration with recombinant E.coli colonies expressing fluorescent proteins and pigments.<br />
<br />
After a brief and interesting introduction by B. Pollak about plasmids, cloning and colony isolation, each of us went on to show a group of 5 kids the picking and striking of bacterial colonies under sterile and safe conditions.<br />
<br />
<html><img src = "https://static.igem.org/mediawiki/2012/d/d2/Pentauc2_uc_chile.jpg" width= "300" align="left" style ="margin:15px"></html>While explaining in simple terms how we obtained purple, green and red bacteria with Violacein synthesis pathway, GFP and RFP genes respectively, the young fellows were given freedom to “paint” with these cells in LB plates (check the results below!)<br />
<br />
After that, we showed them how to visualize the colonies under UV light.<br />
Finally, they were able to identify the differences between green autotrophic bacteria (our beloved Synechocystis PCC6803) and a heterotrophic one (Top10 E.coli) and how that is related to the growing conditions and media.<br />
GFP recombinant chimaeric-corpse engineered H. sapiens<br />
As an iGEM team we felt the experience very important to us because we could share our passion for science with kids that do not have many opportunities to enjoy molecular biology. Also, we could finally leave our tasks for a while and see the reality that surrounds us. This is an eye opener and definitely will help us keep things in perspective. We are extremely happy and grateful for contributing at least for just a few hours to the formation of the future scientists of our country.<br />
<br />
<br />
<h2>Exponential phase constructs</h2> <br />
<br />
<h3>INGENIA fair</h3><br />
<br />
The Faculty of Engineering of our university throws this cool innovation + entrepreneurship fair every year for the last two years. No wait, just the last year. And this one. Okay, so it’s not tradition yet. But it will be. Trust me on this one.<br />
This fair, called INGENIA (a witty pun with Ingeniar, wich would be the verb form of engineering in Spanish) brings some really amazing guests to our yard (huh), including Google, Un techo para chile, Start-up Chile, our Deen and almost every teacher of the faculty who had something amazing to talk about.<br />
And us.<br />
<br />
<html><img src = "https://static.igem.org/mediawiki/2012/4/44/UC_Chile-Ingenia1.png" width= "600" align="right" style ="margin:15px"></html><br />
<br />
I talked to the people in our alumni center, and they were thrilled about the idea of having us there. You see, biotechnology and bioengineering in general don’t get much of a spotlight in our faculty. Most engineers hate biology to their guts, and chemistry kind of makes them barf. I think it’s the lab alcohol, reminding them what they did last summer. And well, every summer.<br />
Back to my point.<br />
When we got the chance of having a booth and a round table for talking about synthetic biology, we couldn’t be happier –and prouder! Making this study-machines (or party-machines, it depends) interested in something other than their specialization field is our own little mission impossible. So when we (I say we, but it was actually our own architect, Rolando, who had to design and design for hours while I sent him about a hundred of versions of the text) decided to make the most amazing posters and handouts, so that at least our stand could be a little flashy. I mean, guys. We’re competing with investigation teams that bring food and wine and beer to their stands. <br />
We had pipettes. I sincerely thought we were screwed.<br />
<br />
These beauties here are our handouts and poster, respectively. Pretty, huh?<br />
<br />
[[File:UC-Chile-Ingenia2.png|left|375 px]][[File:UC_Chile-Ingenia3.png|right|375 px]][[File:UC_Chile-Ingenia4.png|200 px]]<br />
<br />
<br />
<br />
Ok, so we had amazing material. That alone should be enough to make our little booth pretty amazing, right?<br />
Yeah. Not so much.<br />
We also needed PEOPLE.<br />
By 9:49 I was definitely panicking. I had talked to the amazing total of ONE person, and only because he was offering organic chips from his stand. Damn the people who can bring food to their booth. It’s not fair. We could offer bread with luminescent fungus if we wanted okay so just leave us alone. <br />
Anyway. The booth was empty and my nerves were definitely on the edge, but everything changed when the fire nation attacked the 9:50 recess came.<br />
You can always see people flooding out of their classrooms at that exact hour, but this time it was different. We were right in the middle of their trajectory. So they HAD to stop by. And luckily, they did!<br />
We were there for about six hours. We had a little list for people who wanted to receive information about the competition and our projects. Bets came and went through the team members, and the highest bidder said there was no way we could get past twenty people (our faculty is not that big, mind you).<br />
WE HAD 32. 32! AND THEY WERE ALL REAL PEOPLE, NO ANNITA BATHS OR ANYTHING OF SORTS ON THE LIST, I CHECKED.<br />
IT WAS AWESOME.<br />
AWESOME, I SAY.<br />
<br />
<br />
[[File:UC_Chile-Ingenia5.png|235 px]]<br />
[[File:UC_Chile-Ingenia6.png|235 px]]<br />
[[File:UC_Chile-Ingenia7.png|235 px]]<br />
[[File:UC_Chile-Ingenia8.png|235 px]]<br />
<br />
<br />
<br />
[[File:UC_Chile-Ingenia9.png|left|200 px]]<br />
<br />
Most of the people we talked to were engineers specializing in biotechnology, though we did get others interested in math, computer science and mechanical engineering. If they really make it to next year’s team I highly advise you to watch out for them. Awesome interdisciplinary team approaching!<br />
Some of them were really interested in going to the lab to see the results of the projects we talked so much about. If you can do that in your school too, it’s a sure way to interest indecisive people. After all, je pense donc je suis is much more like je vois donc il est, right? <br />
Look at this! We even had the second fullest booth of the fair at some point of the afternoon! We couldn’t beat Google. They had google pencils. I got like five. They were awesome.<br />
It was an amazing day, really.<br />
The next day, we had a round table session to discuss synthetic biology and its influence in real life situations, mostly applied to our life as students. We were filmed and all, so if you’re interested you can watch the footage here: http://twitcam.livestream.com/9pslw<br />
If you and your team have the chance to participate in a similar event, go for it! It’s a great way to get students to know about your projects, iGEM, and synthetic biology in general. Remember to have some nice handouts and a flashy poster. That, or get there dressed up as bacteria, I don’t know! Whatever floats your boat… and helps you get new recruits!<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h3>CONEIB (National reunion of Biotechnology Students)</h3><br />
<br />
<br />
We presented our project at the annual biotechnology students reunion in Antogasta, Chile from October 17 -19. All the people who attended loved our project and wanted to start their own iGEM teams at their universities.<br />
<br />
<br />
<html><br />
Here is<a href="http://www.congresobiotecnologia.cl/" target="_blank"> the official web page of the event!</a></html> (in Spanish).<br />
<br />
<h2>Maturity phase constructs</h2><br />
<br />
<h3> SynBio Workshop</h3><br />
<html><img src = "https://static.igem.org/mediawiki/2012/5/50/Workshop.jpg" width= "650" align="left" style ="margin:15px"><br />
<html><br />
<br />
<a href="https://2012.igem.org/Team:UC_Chile/Workshop"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
Check the next section for [https://2012.igem.org/Team:UC_Chile/Workshop more Workshop details!]<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Community_outreachTeam:UC Chile/Community outreach2012-10-27T01:48:03Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Synthetic Sociology</h1><br />
<html><img src = "https://static.igem.org/mediawiki/2012/a/ad/SYNSOC.2.jpg" width= "970" align="center" style ="margin:15px"></html><br />
Being the first iGEM team in Chile and also part of the only group of students that participated in the first SynBio course held in our country, we faced the challenge of spreading the word. As evangelists, we planned to do with the Chilean society something analog to what we did in our project, placing our desired outputs (getting people into SynBio) downstream the endogenous machinery (schools, fairs, faculties, etc): Welcome to Synthetic Sociology.<br />
<br />
<h2>Early phase constructs: Penta UC Kids</h2><br />
<br />
<html><img src = "https://static.igem.org/mediawiki/2012/4/44/Pentauc1_uc_chile.jpg" width= "300" align="right" style ="margin:15px"></html><br />
The Talents Development and Study Program at Catholic University (PENTA UC for its initials in spanish) aims to educate and form creative and proactive kids and to reinforce their passion for knowledge. At the same time the PENTA program helps to enrich their personal and social maturation process through the development of confidence in themselves and their capacities, together with an ethical and socially responsible attitude.<br />
<br />
Through a curriculum of multiple disciplines and practical workshops lead by UC professors and students the program gives kids from socially disadvantaged contexts the opportunity to develop their potential.<br />
<br />
One of the courses, called “What if Dr. Frankenstein lived today?” is designed to introduce 13 year old kids to the practical and theoretical fundamentals of genetic engineering while addressing ethical questions in order to understand how it is possible to work with genes and use them for the benefit of mankind.<br />
<br />
This course happened to be guided by Ariel Herrera, a graduate student who is pursuing his Ph.D in the lab next to ours. We agreed that it would be great to explain the Central Dogma of molecular biology through a practical demonstration with recombinant E.coli colonies expressing fluorescent proteins and pigments.<br />
<br />
After a brief and interesting introduction by B. Pollak about plasmids, cloning and colony isolation, each of us went on to show a group of 5 kids the picking and striking of bacterial colonies under sterile and safe conditions.<br />
<br />
<html><img src = "https://static.igem.org/mediawiki/2012/d/d2/Pentauc2_uc_chile.jpg" width= "300" align="left" style ="margin:15px"></html>While explaining in simple terms how we obtained purple, green and red bacteria with Violacein synthesis pathway, GFP and RFP genes respectively, the young fellows were given freedom to “paint” with these cells in LB plates (check the results below!)<br />
<br />
After that, we showed them how to visualize the colonies under UV light.<br />
Finally, they were able to identify the differences between green autotrophic bacteria (our beloved Synechocystis PCC6803) and a heterotrophic one (Top10 E.coli) and how that is related to the growing conditions and media.<br />
GFP recombinant chimaeric-corpse engineered H. sapiens<br />
As an iGEM team we felt the experience very important to us because we could share our passion for science with kids that do not have many opportunities to enjoy molecular biology. Also, we could finally leave our tasks for a while and see the reality that surrounds us. This is an eye opener and definitely will help us keep things in perspective. We are extremely happy and grateful for contributing at least for just a few hours to the formation of the future scientists of our country.<br />
<br />
<br />
<h2>Exponential phase constructs</h2> <br />
<br />
<h3>INGENIA fair</h3><br />
<br />
The Faculty of Engineering of our university throws this cool innovation + entrepreneurship fair every year for the last two years. No wait, just the last year. And this one. Okay, so it’s not tradition yet. But it will be. Trust me on this one.<br />
This fair, called INGENIA (a witty pun with Ingeniar, wich would be the verb form of engineering in Spanish) brings some really amazing guests to our yard (huh), including Google, Un techo para chile, Start-up Chile, our Deen and almost every teacher of the faculty who had something amazing to talk about.<br />
And us.<br />
<br />
<html><img src = "https://static.igem.org/mediawiki/2012/4/44/UC_Chile-Ingenia1.png" width= "600" align="right" style ="margin:15px"></html><br />
<br />
I talked to the people in our alumni center, and they were thrilled about the idea of having us there. You see, biotechnology and bioengineering in general don’t get much of a spotlight in our faculty. Most engineers hate biology to their guts, and chemistry kind of makes them barf. I think it’s the lab alcohol, reminding them what they did last summer. And well, every summer.<br />
Back to my point.<br />
When we got the chance of having a booth and a round table for talking about synthetic biology, we couldn’t be happier –and prouder! Making this study-machines (or party-machines, it depends) interested in something other than their specialization field is our own little mission impossible. So when we (I say we, but it was actually our own architect, Rolando, who had to design and design for hours while I sent him about a hundred of versions of the text) decided to make the most amazing posters and handouts, so that at least our stand could be a little flashy. I mean, guys. We’re competing with investigation teams that bring food and wine and beer to their stands. <br />
We had pipettes. I sincerely thought we were screwed.<br />
<br />
These beauties here are our handouts and poster, respectively. Pretty, huh?<br />
<br />
[[File:UC-Chile-Ingenia2.png|left|375 px]][[File:UC_Chile-Ingenia3.png|right|375 px]][[File:UC_Chile-Ingenia4.png|200 px]]<br />
<br />
<br />
<br />
Ok, so we had amazing material. That alone should be enough to make our little booth pretty amazing, right?<br />
Yeah. Not so much.<br />
We also needed PEOPLE.<br />
By 9:49 I was definitely panicking. I had talked to the amazing total of ONE person, and only because he was offering organic chips from his stand. Damn the people who can bring food to their booth. It’s not fair. We could offer bread with luminescent fungus if we wanted okay so just leave us alone. <br />
Anyway. The booth was empty and my nerves were definitely on the edge, but everything changed when the fire nation attacked the 9:50 recess came.<br />
You can always see people flooding out of their classrooms at that exact hour, but this time it was different. We were right in the middle of their trajectory. So they HAD to stop by. And luckily, they did!<br />
We were there for about six hours. We had a little list for people who wanted to receive information about the competition and our projects. Bets came and went through the team members, and the highest bidder said there was no way we could get past twenty people (our faculty is not that big, mind you).<br />
WE HAD 32. 32! AND THEY WERE ALL REAL PEOPLE, NO ANNITA BATHS OR ANYTHING OF SORTS ON THE LIST, I CHECKED.<br />
IT WAS AWESOME.<br />
AWESOME, I SAY.<br />
<br />
<br />
[[File:UC_Chile-Ingenia5.png|235 px]]<br />
[[File:UC_Chile-Ingenia6.png|235 px]]<br />
[[File:UC_Chile-Ingenia7.png|235 px]]<br />
[[File:UC_Chile-Ingenia8.png|235 px]]<br />
<br />
<br />
<br />
[[File:UC_Chile-Ingenia9.png|left|200 px]]<br />
<br />
Most of the people we talked to were engineers specializing in biotechnology, though we did get others interested in math, computer science and mechanical engineering. If they really make it to next year’s team I highly advise you to watch out for them. Awesome interdisciplinary team approaching!<br />
Some of them were really interested in going to the lab to see the results of the projects we talked so much about. If you can do that in your school too, it’s a sure way to interest indecisive people. After all, je pense donc je suis is much more like je vois donc il est, right? <br />
Look at this! We even had the second fullest booth of the fair at some point of the afternoon! We couldn’t beat Google. They had google pencils. I got like five. They were awesome.<br />
It was an amazing day, really.<br />
The next day, we had a round table session to discuss synthetic biology and its influence in real life situations, mostly applied to our life as students. We were filmed and all, so if you’re interested you can watch the footage here: http://twitcam.livestream.com/9pslw<br />
If you and your team have the chance to participate in a similar event, go for it! It’s a great way to get students to know about your projects, iGEM, and synthetic biology in general. Remember to have some nice handouts and a flashy poster. That, or get there dressed up as bacteria, I don’t know! Whatever floats your boat… and helps you get new recruits!<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h3>CONEIB (National reunion of Biotechnology Students)</h3><br />
<br />
<br />
We presented our project at the annual biotechnology students reunion in Antogasta, Chile from October 17 -19. All the people who attended loved our project and wanted to start their own iGEM teams at their universities.<br />
<br />
<br />
<html><br />
Here is<a href="http://www.congresobiotecnologia.cl/" target="_blank"> the official web page of the event!</a></html> (in Spanish).<br />
<br />
<h2>Maturity phase constructs</h2><br />
<br />
<h3> SynBio Workshop</h3><br />
<html><img src = "https://static.igem.org/mediawiki/2012/5/50/Workshop.jpg" width= "650" align="left" style ="margin:15px"><br />
<html><br />
<br />
<a href="https://2012.igem.org/Team:UC_Chile/Workshop"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
Check the next section for [https://2012.igem.org/Team:UC_Chile/Workshop more details!]<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Community_outreachTeam:UC Chile/Community outreach2012-10-27T01:47:26Z<p>Csvidal: </p>
<hr />
<div>{{UC_Chile4}}<br />
<br />
<h1>Synthetic Sociology</h1><br />
<html><img src = "https://static.igem.org/mediawiki/2012/a/ad/SYNSOC.2.jpg" width= "970" align="center" style ="margin:15px"></html><br />
Being the first iGEM team in Chile and also part of the only group of students that participated in the first SynBio course held in our country, we faced the challenge of spreading the word. As evangelists, we planned to do with the Chilean society something anallog to what we did in our project, placing our desired outputs (getting people into SynBio) downstream the endogenous machinery (schools, fairs, faculties, etc): Welcome to Synthetic Sociology.<br />
<br />
<h2>Early phase constructs: Penta UC Kids</h2><br />
<br />
<html><img src = "https://static.igem.org/mediawiki/2012/4/44/Pentauc1_uc_chile.jpg" width= "300" align="right" style ="margin:15px"></html><br />
The Talents Development and Study Program at Catholic University (PENTA UC for its initials in spanish) aims to educate and form creative and proactive kids and to reinforce their passion for knowledge. At the same time the PENTA program helps to enrich their personal and social maturation process through the development of confidence in themselves and their capacities, together with an ethical and socially responsible attitude.<br />
<br />
Through a curriculum of multiple disciplines and practical workshops lead by UC professors and students the program gives kids from socially disadvantaged contexts the opportunity to develop their potential.<br />
<br />
One of the courses, called “What if Dr. Frankenstein lived today?” is designed to introduce 13 year old kids to the practical and theoretical fundamentals of genetic engineering while addressing ethical questions in order to understand how it is possible to work with genes and use them for the benefit of mankind.<br />
<br />
This course happened to be guided by Ariel Herrera, a graduate student who is pursuing his Ph.D in the lab next to ours. We agreed that it would be great to explain the Central Dogma of molecular biology through a practical demonstration with recombinant E.coli colonies expressing fluorescent proteins and pigments.<br />
<br />
After a brief and interesting introduction by B. Pollak about plasmids, cloning and colony isolation, each of us went on to show a group of 5 kids the picking and striking of bacterial colonies under sterile and safe conditions.<br />
<br />
<html><img src = "https://static.igem.org/mediawiki/2012/d/d2/Pentauc2_uc_chile.jpg" width= "300" align="left" style ="margin:15px"></html>While explaining in simple terms how we obtained purple, green and red bacteria with Violacein synthesis pathway, GFP and RFP genes respectively, the young fellows were given freedom to “paint” with these cells in LB plates (check the results below!)<br />
<br />
After that, we showed them how to visualize the colonies under UV light.<br />
Finally, they were able to identify the differences between green autotrophic bacteria (our beloved Synechocystis PCC6803) and a heterotrophic one (Top10 E.coli) and how that is related to the growing conditions and media.<br />
GFP recombinant chimaeric-corpse engineered H. sapiens<br />
As an iGEM team we felt the experience very important to us because we could share our passion for science with kids that do not have many opportunities to enjoy molecular biology. Also, we could finally leave our tasks for a while and see the reality that surrounds us. This is an eye opener and definitely will help us keep things in perspective. We are extremely happy and grateful for contributing at least for just a few hours to the formation of the future scientists of our country.<br />
<br />
<br />
<h2>Exponential phase constructs</h2> <br />
<br />
<h3>INGENIA fair</h3><br />
<br />
The Faculty of Engineering of our university throws this cool innovation + entrepreneurship fair every year for the last two years. No wait, just the last year. And this one. Okay, so it’s not tradition yet. But it will be. Trust me on this one.<br />
This fair, called INGENIA (a witty pun with Ingeniar, wich would be the verb form of engineering in Spanish) brings some really amazing guests to our yard (huh), including Google, Un techo para chile, Start-up Chile, our Deen and almost every teacher of the faculty who had something amazing to talk about.<br />
And us.<br />
<br />
<html><img src = "https://static.igem.org/mediawiki/2012/4/44/UC_Chile-Ingenia1.png" width= "600" align="right" style ="margin:15px"></html><br />
<br />
I talked to the people in our alumni center, and they were thrilled about the idea of having us there. You see, biotechnology and bioengineering in general don’t get much of a spotlight in our faculty. Most engineers hate biology to their guts, and chemistry kind of makes them barf. I think it’s the lab alcohol, reminding them what they did last summer. And well, every summer.<br />
Back to my point.<br />
When we got the chance of having a booth and a round table for talking about synthetic biology, we couldn’t be happier –and prouder! Making this study-machines (or party-machines, it depends) interested in something other than their specialization field is our own little mission impossible. So when we (I say we, but it was actually our own architect, Rolando, who had to design and design for hours while I sent him about a hundred of versions of the text) decided to make the most amazing posters and handouts, so that at least our stand could be a little flashy. I mean, guys. We’re competing with investigation teams that bring food and wine and beer to their stands. <br />
We had pipettes. I sincerely thought we were screwed.<br />
<br />
These beauties here are our handouts and poster, respectively. Pretty, huh?<br />
<br />
[[File:UC-Chile-Ingenia2.png|left|375 px]][[File:UC_Chile-Ingenia3.png|right|375 px]][[File:UC_Chile-Ingenia4.png|200 px]]<br />
<br />
<br />
<br />
Ok, so we had amazing material. That alone should be enough to make our little booth pretty amazing, right?<br />
Yeah. Not so much.<br />
We also needed PEOPLE.<br />
By 9:49 I was definitely panicking. I had talked to the amazing total of ONE person, and only because he was offering organic chips from his stand. Damn the people who can bring food to their booth. It’s not fair. We could offer bread with luminescent fungus if we wanted okay so just leave us alone. <br />
Anyway. The booth was empty and my nerves were definitely on the edge, but everything changed when the fire nation attacked the 9:50 recess came.<br />
You can always see people flooding out of their classrooms at that exact hour, but this time it was different. We were right in the middle of their trajectory. So they HAD to stop by. And luckily, they did!<br />
We were there for about six hours. We had a little list for people who wanted to receive information about the competition and our projects. Bets came and went through the team members, and the highest bidder said there was no way we could get past twenty people (our faculty is not that big, mind you).<br />
WE HAD 32. 32! AND THEY WERE ALL REAL PEOPLE, NO ANNITA BATHS OR ANYTHING OF SORTS ON THE LIST, I CHECKED.<br />
IT WAS AWESOME.<br />
AWESOME, I SAY.<br />
<br />
<br />
[[File:UC_Chile-Ingenia5.png|235 px]]<br />
[[File:UC_Chile-Ingenia6.png|235 px]]<br />
[[File:UC_Chile-Ingenia7.png|235 px]]<br />
[[File:UC_Chile-Ingenia8.png|235 px]]<br />
<br />
<br />
<br />
[[File:UC_Chile-Ingenia9.png|left|200 px]]<br />
<br />
Most of the people we talked to were engineers specializing in biotechnology, though we did get others interested in math, computer science and mechanical engineering. If they really make it to next year’s team I highly advise you to watch out for them. Awesome interdisciplinary team approaching!<br />
Some of them were really interested in going to the lab to see the results of the projects we talked so much about. If you can do that in your school too, it’s a sure way to interest indecisive people. After all, je pense donc je suis is much more like je vois donc il est, right? <br />
Look at this! We even had the second fullest booth of the fair at some point of the afternoon! We couldn’t beat Google. They had google pencils. I got like five. They were awesome.<br />
It was an amazing day, really.<br />
The next day, we had a round table session to discuss synthetic biology and its influence in real life situations, mostly applied to our life as students. We were filmed and all, so if you’re interested you can watch the footage here: http://twitcam.livestream.com/9pslw<br />
If you and your team have the chance to participate in a similar event, go for it! It’s a great way to get students to know about your projects, iGEM, and synthetic biology in general. Remember to have some nice handouts and a flashy poster. That, or get there dressed up as bacteria, I don’t know! Whatever floats your boat… and helps you get new recruits!<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<h3>CONEIB (National reunion of Biotechnology Students)</h3><br />
<br />
<br />
We presented our project at the annual biotechnology students reunion in Antogasta, Chile from October 17 -19. All the people who attended loved our project and wanted to start their own iGEM teams at their universities.<br />
<br />
<br />
<html><br />
Here is<a href="http://www.congresobiotecnologia.cl/" target="_blank"> the official web page of the event!</a></html> (in Spanish).<br />
<br />
<h2>Maturity phase constructs</h2><br />
<br />
<h3> SynBio Workshop</h3><br />
<html><img src = "https://static.igem.org/mediawiki/2012/5/50/Workshop.jpg" width= "650" align="left" style ="margin:15px"><br />
<html><br />
<br />
<a href="https://2012.igem.org/Team:UC_Chile/Workshop"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"><br />
</html><br />
Check the next section for [https://2012.igem.org/Team:UC_Chile/Workshop more details!]<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_ChileTeam:UC Chile2012-10-26T22:56:57Z<p>Csvidal: </p>
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<div>{{UC_Chile4}}<br />
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<div class="slider-wrapper theme-default"><br />
<div class="ribbon"></div><br />
<div id="slider" class="nivoSlider"><br />
<img src="https://static.igem.org/mediawiki/2012/7/7a/UC_Chile-Bioluminescence.jpg" alt="" title="#htmlcaption1"/><br />
<img src="https://static.igem.org/mediawiki/2012/5/5a/UC_Chile-Syne.jpg" alt="" title="#htmlcaption2"/><br />
<img src="https://static.igem.org/mediawiki/2012/f/fa/UC-Chile-Constructs.jpg" alt="" title="#htmlcaption4"/><br />
<img src="https://static.igem.org/mediawiki/2012/a/a1/UC_Chile-Transformation2.png" alt="" title="#htmlcaption5"/><br />
<img src="https://static.igem.org/mediawiki/2012/2/29/Al_Bio.jpg" alt="" title="#htmlcaption" /><br />
<img src="https://static.igem.org/mediawiki/2012/b/bc/Teamchilepenta_uc_chile.jpg" alt="" title="#htmlcaption4"/><br />
<img src="https://static.igem.org/mediawiki/2012/c/c0/UC_Chile-Brainstorming.jpg" alt="" /><br />
<img src="https://static.igem.org/mediawiki/2012/b/ba/Growthcurve_uc_chile.jpg" alt="" /><br />
<img src="https://static.igem.org/mediawiki/2012/1/1d/Syne_Transformation.JPG" alt="" /><br />
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<div id="htmlcaption1" class="nivo-html-caption"><br />
<center style="font-family:'Vollkorn', serif; color: white;">Luxilla Biolamp takes advantage of one the most fascinating natural phenomena: bioluminescence. </center></div><br />
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<div id="htmlcaption2" class="nivo-html-caption"><br />
<center style="font-family:'Vollkorn', serif; color: white;">We chose for the chassis the cyanobacteria Synechocystis PCC 6803, because it is a photoautotroph and is regulated by circadian rhythms.</center></div><br />
<br />
<div id="htmlcaption4" class="nivo-html-caption"><br />
<center style="font-family:'Vollkorn', serif; color: white;">Using the lux operon and circadian promoters, we designed two plasmids: the Luciferase generator is to produce bioluminiscence, and the substrate generator recovers substainably the substrates necessary for the reactions.</center></div><br />
<br />
<div id="htmlcaption5" class="nivo-html-caption"><br />
<center style="font-family:'Vollkorn', serif; color: white;">We achieved to transform Synechocystis with the luciferase generator plasmid, and verificated light emission in a luminometer.</center></div><br />
<br />
<br />
<div id="htmlcaption" class="nivo-html-caption"><br />
<center style="font-family:'Vollkorn', serif; color: white;">Al being lit by bioluminescent bacteria! </center></div><br />
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<div id="Cyano"><br />
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<td><a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Project"><img src="https://static.igem.org/mediawiki/2012/f/f9/UC_Chile-Animated-abstract-final.gif" align="left"><br />
<img src="https://static.igem.org/mediawiki/2012/5/5f/UC_ChileSide-projects3_-_title.jpg" align="right"><br />
<a href="https://2012.igem.org/Team:UC_Chile/Bactomithril"><img src="https://static.igem.org/mediawiki/2012/d/d6/UC_ChileSide-projects3_-_bacto.jpg" align="right"><br />
<a href="https://2012.igem.org/Team:UC_Chile/Results/Gibson"><img src="https://static.igem.org/mediawiki/2012/0/02/UC_ChileSide-projects3_-_gibson.jpg" align="right"><br />
<a href="https://2012.igem.org/Team:UC_Chile/Results/LuxBrick"><img src="https://static.igem.org/mediawiki/2012/4/4e/UC_ChileSide-projects3_-_lux.jpg" align="right"><br />
<a href="https://2012.igem.org/Team:UC_Chile/Results/Growthcurve"><img src="https://static.igem.org/mediawiki/2012/a/a8/UC_CHileSide-projects3_-_growth.jpg" align="right"><a href="https://2012.igem.org/Team:UC_Chile/Results/Int_C"><img src="https://static.igem.org/mediawiki/2012/f/f1/UC_ChileSide-projects3_-_debugging.jpg" align="right"><a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Future"><img src="https://static.igem.org/mediawiki/2012/e/e9/UC_ChileSide-projects3_-_gfp.jpg" align="right"></td><br />
</tr><br />
</table><br />
<center><br />
</html><br />
[[File:Results_jamboree_igem_uc_chile2.jpg]]<br />
<br><br><br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Labook/marchTeam:UC Chile/Labook/march2012-10-26T19:17:01Z<p>Csvidal: </p>
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<div id="navbar3"><br />
<ul>Lab book:<br> <br />
<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/october">October</a></li><br />
</ul><br />
</div><br />
</html><br />
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<br />
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<br />
<p><font size= "5">March</font></p><br />
<br><br><br />
<font size="4">Monthly Summary:</font><br />
<br />
<br />
We started with the design of constructs to express RFP under circadian promoters (psbAB and psBA2) and their respective primers. The amplification of the parts needed for the constructs are obtained by PCR. There have been several problems related to the amplification of Kanr. The PCR has been repeated with high frequency with no significant results.<br />
<br />
As we decided to do two different projects, the team was divided into subgroups and each person has specific tasks. These are mainly related to dry and wet lab areas.<br />
<br />
<br />
<font size="4">Constructs and primers design</font> <br />
<br />
<br />
First constructs. Check: https://2012.igem.org/Team:UC_Chile2/Cyanolux/Biobricks <br />
<br />
Strategy for integration construct: <br />
Part K292003 is not in the kit. It will be assembled from P1003+B0012+B0014 <br />
<br />
<br />
<font size="4">Getting parts ready</font><br />
<br />
<br />
Plates prepared<br />
Broad host plasmid transformation <br />
Batch of new competent bacteria ready<br />
Primer alicuoting (primers asked for in late January). <br />
<br />
Amplification of parts from 2011 kit: P1003, B0014 and others (specify) are needed for constructs.<br><br />
Standard Biobrick assembly.<br> <br />
<br><br />
Transformation.<br><br />
Plated, only one surviving colony.<br><br />
Conclusion: psB1C3 was already CUT<br><br />
<br><br />
PCR’s. By electrophoresis we have:<br><br />
Unsuccessful attempts: psbAB,psbAB2, Kanr, RS1.<br><br />
Success: RFP, RS2, backbone.<br><br />
Comment: seems as primers for expression plasmid are problematic.<br><br />
Kanr new PCR attempts. Unsuccessful results.<br><br />
PCR for pPMQAK1, psbAB, RFP.<br> <br />
<br />
<br />
<font size="4">Work plan</font><br />
<br />
<br />
Labour division: Bactomithril {Max, Emilia,Ulises, Claudia, Bryon}<br><br />
Cyano {Simón, Carla, Tamara, Isaac, Sebastián}<br />
<br />
<br />
Meetings with Dr. Gutiérrez every two weeks to check work done. <br />
<br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyano/Labook/aprilTeam:UC Chile/Cyano/Labook/april2012-10-26T19:16:19Z<p>Csvidal: </p>
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<br><br />
<div id="navbar3"><br />
<ul>Lab book:<br> <br />
<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/october">October</a></li><br />
</ul><br />
</div><br />
</html><br />
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<br />
<br />
<p><font size= "5">April</font></p><br />
<br />
<br />
<font size="4">Monthly Summary</font><br />
<br><br><br />
PCR runs have been defective so we haven’t been able to obtain all the parts needed to assemble our constructs. Nonetheless, parts for C1.1 and C2.1 amplified and a Gibson assembly was attempted to join them. The assembly was incorrect according to a restriction enzyme digestion protocol.<br />
<br />
Synechocytis are growing ok.<br />
<br />
<br />
<br />
<font size="4"><i>Synechocystis PCC6803</i></font><br />
<br><br><br />
Attempted synechocytis DNA extraction according to protocol. There was no pellet and no DNA.<br />
<br />
Synechocystis cultures were observed under acridine dye and turned out to be axenic. (upload photos). Cultures were reinoculated in fresh BG11.<br />
<br />
Started a growth curve for Synechocystis (that was never finished).<br />
<br />
<br />
<br />
<font size="4">PCR's</font><br />
<br><br />
<br><br />
* 17 PCR’s were run. Some parts amplified correctly and some did not.<br />
Comments: DNA from synechocystis as template is not good enough. Primers form secondary images of these structures)<br />
<br />
<br />
<br />
<font size="4">Gibson Assembly</font><br />
<br />
<br />
Constructs whose all parts could be obtained by PCR: C1.1 and C2.1. The Gibson assembly technique was used to build the constructs. Transformation followed. <br />
<br />
Results: C1.1≈ 7 colonies<br><br />
C2.1≈ 5 colonies<br><br />
B1 ≈ 1 colony (bacto construct)<br><br />
sfGFP (control) ≈ 15, all red (contamination?)<br><br />
<br />
A DNA extraction and a restriction enzyme protocols were done in order to verify constructs.<br />
<br />
Cut regions: C1.1 -> psbAB-RFP<br><br />
C 2.1 -> psbA2-RFP<br />
<br />
Results: ?? (I believe size was wrong)<br />
<br />
<br />
<br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyano/Labook/mayTeam:UC Chile/Cyano/Labook/may2012-10-26T19:15:32Z<p>Csvidal: </p>
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<br><br />
<div id="navbar3"><br />
<ul>Lab book:<br> <br />
<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/october">October</a></li><br />
</ul><br />
</div><br />
</html><br />
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<br />
<br />
<p><font size= "5">May</font></p><br />
<br />
<br />
<font size="4">Monthly Summary</font><br />
<br><br />
<br><br />
As Gibson assemblies are not working properly, constructs are being prepared in parallel by Standard Biobrick Assembly. After many attempts we concluded that the enzymes in the master mix are defective and new ones were ordered. There will be no Gibson attempts until arrival.<br />
<br />
<br />
<font size="4">Digestion and Ligation attempts</font><br />
<br />
<br />
pPMQAK1 (expression plasmid) was miniprepped and transformed.<br />
<br />
The plan was to get ccab toxin out of the plasmid for further use. <br />
<br />
1st attempt: negative control (pPMQAK1 with toxin) was positive. <br />
<br />
Comments: possible reasons? Contamination, other resistance, toxin is not toxic. Plasmid sent is not pPMQAK1. <br />
<br />
pPMQAK1 was digested.<br />
<br />
Colony PCR for C1/2, C1/2 -, C1<br />
<br />
New primers arrived:<br />
<br />
New PCR attempts and new transformations. <br />
<br />
Started to assemble constructs containing Lux, GFP and RFP. <br />
<br />
Standard biobrick assembly was attempted (x2 times). There were no colonies in transformation. <br />
<br />
<br />
<font size="4">Gibson Assembly Attempts</font><br />
<br><br />
<br><br />
<br />
PCR for Kanr was repeated.<br />
<br />
F. Federici suggested a way to assemble constructs using psB4K5 (steps must be detailed).<br />
<br />
Digestion of double terminator (P1003+B0012+B0014) was done to verify assembly.<br />
<br />
Transformation according to Federici’s suggestion: there were no colonies in negative control.<br />
<br />
One last big PCR purification to attempt assembly of C1 and C1/2. Gibson assembly and transformation. <br />
<br />
Results: no colonies.<br />
<br />
A Gibson assembly with sfGFP and December’s master mix (we know it works) was done in order to check the efficiency of the method. After transformation no colonies were observed.<br />
<br />
Comments: Gibson current master mix could be wrong.<br />
<br />
Restriction and analytic digestion of P1003, B0014 and psB1C3 revealed correct parts size (these will be used for a new Gibson). New Gibson Master Mix prepared. Bacteria with new Master Mix: no colonies.<br />
<br />
Another possibly reason for the unsuccess of Gibson assembly could be that the T5 enzyme chews back an T5 enzyme chews back an important amount of base pairs and would be detrimental for the parts with small length.<br />
<br />
An experiment trying Gibson assembly with different T5 concentrations was done. Best yield at: +10 ^ (1/2)x<br />
<br />
Cell competence was measured: 2.5x10^5 (low)<br />
<br />
Attempted a Gibson with all possible parts. No colonies and no colonies in positive control were observed after transformation.<br />
Comments: we believe that the enzymes for the reaction are defective. New enzymes were ordered. No new Gibson assemblies will be attempted until the arrival.<br />
<br />
<br />
<br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyano/Labook/juneTeam:UC Chile/Cyano/Labook/june2012-10-26T19:15:01Z<p>Csvidal: </p>
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<div id="navbar3"><br />
<ul>Lab book:<br> <br />
<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/october">October</a></li><br />
</ul><br />
</div><br />
</html><br />
<br />
<br />
<p><font size= "5">June</font></p><br />
<br />
<br />
<font size="4">Monthly Summary</font><br />
<br />
<br />
We are waiting for Gibson enzymes to arrive. So there is no much wet lab work this month. <br />
<br />
<br />
<font size="4">PCR runs</font><br />
<br />
<br />
While waiting for enzymes, all parts for future assemblies are being prepared.<br />
<br />
Problematic parts: Kanr, P1003, B0014.<br />
<br />
Tried with different DMSO concentrations. Best result at 5%.<br />
<br />
Comments: we realized that our current primers work for P1003+B0015. Right primers were now ordered.<br />
<br />
After arrival, no amplification for Kanr. Tried once more with an old Kanr purification: success.<br />
<br />
New batch of competent cells prepared: Competence: 3.5x10^7 (not quite sure)<br />
<br />
Gibson enzymes arrived late June.<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyano/Labook/julyTeam:UC Chile/Cyano/Labook/july2012-10-26T19:14:23Z<p>Csvidal: </p>
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<ul>Lab book:<br> <br />
<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/october">October</a></li><br />
</ul><br />
</div><br />
</html><br />
<br />
<br />
<p><font size= "5">July</font></p><br />
<br />
<br />
- The following digestions were done:<br><br><br />
RS1 + Kanr + psB1C3 <br><br />
RS1 + Kanr <br><br />
B0014 + RS2 + psB1C3<br><br />
B0014 + RS2 <br><br />
<br />
There were colonies in all the plates. They were cultured.<br><br />
<br />
- Did a PCR run of LuxCDEG. Gel from electrophoresis showed: nothing.<br><br />
- Gel with LuxCDEG (cut wit X+P and uncut): nothing. Ligations must be incorrect.<br><br />
- Prepared all the material to send to Fernán at Cambridge.<br><br />
<br />
The following ligations were done.<br><br />
<br />
(Lux CDEG1 + E + S) digestion + (Lux CDEG2 + X + P) digestion + (psB1C3 + E+ P) digestion.<br><br />
<br />
Colonies did not grow on Kanamycin plates. Negative colony PCR.<br><br />
New digestion: LuxCD, LuxEG + terminator.<br><br />
Negative colony PCR for RS2 + B0014 + psB4K5. <br><br />
<br />
We could not identify LuxCD and LuxEG from VB. Anyway, they were ligated and transformed.<br><br />
<br />
Synechocystis transformation:<br><br />
<br />
psB1C3_IntC and RFP<br><br />
For the psbAB + GFP transformation the synechocystis transformation protocol was followed until plating.<br><br />
Colony PCR for LuxCEDG, B0014 + RS2 + KanR and psB4K5 + sfGFP: new failed attempt. New strategy: purification of RS2 and B0014 and B0015.<br><br />
<br />
As B0014 is problematic the part was switched for B0015. The part was digested.<br><br />
Also digested RS2 with (E + P) and ligated with B0015. Transformation and plating followed.<br><br />
<br />
Another ligation to assemble: (RS1 + Kan) (E + S) + (RS2)(X + P)... LuxCD + LuxEG to intC. <br><br />
PCR for LuxCDEG plasmid.<br><br />
<br />
Parts purified (RS1 + Kan) , RS2, B0014 and B0015.<br><br />
<br />
Ligations: (RS1 + Kan) + RS2 in psB1A2<br><br />
(RS2 + B0014) in psB1C3<br><br />
(RS2 + B0015) in psB1C3<br><br />
LuxCD + LuxEG in Int_C<br><br />
<br />
New PCR for LuxCD and LuxEG with VF (suffix R_digest) and VR (prefix F_digest)<br><br />
LuxCD looks faint, LuxEG is good. Band LuxEG was extracted.<br><br />
<br />
Gel with colony PCR RS2 cut (no restriction site) and LuxCDEG.<br><br />
The bands with normal size were ligated to B0014 and B0015.<br><br />
<br />
PCR runs for psB1C3, psB1T3, psB1K3, psB1K5 and LuxCD.<br><br />
<br />
Colony PCR for ligations (included RS1 + RS2) All had correct size except LUX CD + EG)<br><br />
Finally digested LuxCD from Brick and Lux EG from PCR. LuxCD: size was correct, ligated back and transformed.<br><br />
Minipreps for colony PCR. DNA was extracted, parts were digested and ligated.<br><br />
<br />
RS1 + Kan + B0014 + RS2 in psB1C3<br><br />
RS1 + Kan + B0015 + RS2 in psB1C3<br><br />
<br />
Will be used to insert parts between RS1 and Kan by Gibson.<br><br />
<br />
Different PCR's to obtain: VB_pBAD, RS1 + RS2, Lux CD, psB1A3, pSb1t3. Low yield (except VB_pBAD and RS1 and RS2). PCR was done with this parts as template to amplify back.<br><br />
<br />
Colony PCR for Lux ligations, RS1 + Kan + B0014 + RS2 in psB1C3 and RS1 + Kan + B0015 + RS2 in psB1C3.<br><br />
Right size: RS1 + Kan + B0014 + RS2 + psB1C3<br><br />
RS2 + Kan + B0015 + RS2 + psB1C3<br><br />
<br />
Lux CDEG will be miniprepped and size will be checked.<br><br />
<br />
New PCR for parts: ADF-3, psB1C3, LuxAB<br><br />
<br />
minipreps: LuxCD, LuxCDEG, RS1 Kan r + B0015 + RS2, RS1 + Kanr + B0015 +RS2, RS1 + Kanr + B0014 + RS2.<br><br />
<br />
By verification digest (E + P):<br><br />
LUXCDEG wrong size, B0015 wrong size, B0015 right size (colony 1).<br><br />
C4 is ready!<br><br />
<br />
So now we have 3 basic problems.<br><br />
- Not sure if primers amplified our pieces (if done with preffix, suffix, no restriction enzyme will join).<br><br />
- When digest CD and EG lots of pieces are loose. As ligase also nicks blunt ends, there are lots of false positives<br><br />
- CD, EG and psB1C3 have the same size so they can't be told apart by electrophoresis. New ways to ligate LuxCDEG:<br><br><br />
1. amplify CD and EG (VF2 and VR)<br><br />
2. digest LuxCD (E+S), LuxEG (X+P)<br><br />
3. Electrophoresis (now parts can be distinguished)<br><br />
and/or<br><br />
4. ligate digest LuxCD from plasmid (S+P)<br><br />
5. ligate with LuxEG<br><br><br />
<br />
PCR for: translado promoter, Pcaa3, LuxAB, VB (psB1C3, psB1K3, psB1A2, pSB1T3)<br><br />
So, PCR and electrophoresis for LuxCD and EG (right size).<br><br />
New batch of competent cells.<br><br />
Ligation LuxCD + LuxEG + psB4K5<br><br />
LuxCD + LuxEG + psB1K3<br><br />
<br />
New Gibson attempt. sfGFP in psB4K5 (just a try out for new competent cells)<br><br />
Transformation in pUC 19<br><br />
PUC had colonies. Ligations with colonies. Positive control for Gibson (sfGFP in psB4K5) turn out to be positive :)<br />
So the problem was: E. cloni instead of TOP 10.<br><br />
Colony PCR for LuxCDEG in psB4K5. Had red colonies in it.<br><br />
PCR for RS1_Kanr_B0015_RS2<br><br />
As primers form dimers the PCR was done at hgher Tm<br><br />
amplification of RS1+ Kan + B001 + RS2 --> right size<br><br />
<br />
<br />
<br />
<br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyano/Labook/augustTeam:UC Chile/Cyano/Labook/august2012-10-26T19:13:51Z<p>Csvidal: </p>
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<ul>Lab book:<br> <br />
<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/october">October</a></li><br />
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<br />
<p><font size= "5">August</font></p><br />
<br />
During the last days of July we assembled and got PCR confirmation of BBa_K743006 (RS1-KanR-B0015-RS2). The construct was purified and now is part of our BioBrick collection.<br />
<br />
Parts C4 construct, psbAB (Synechocystis promoter) and RFP marker were assembled by Gibson and subsequently transformed. In this way we obtained BBa_K743004 (RS1-psbAB-mRFP-B0015-KanR-B0015-RS2). Confirmation was positive by PCR and digestion.<br />
<br />
Device (BBa_K743004) was transformed into cells and then purified. Later it was used to amplify its vector backbone from RS2 to mRFP. By doing this we wanted to exchange part KanR for KanRi (KanR inverse).<br />
The new amplicon was purified (VB to mRFP) and was assembled to KanRi part by Gibson, obtaining the device BBa_K743009 (RS1-psbAB-mRFP-B0015-KanRi-B0015-RS2). This new construct was transformed into cells and subsequently results were confirmed by PCR and digestion.<br />
<br />
In addition, we obtained circadian promoters pta, pcaa3 and psigE by PCR from Synechocystis DNA. LuxAB from Xenorhabdus luminescens was amplified. By assembly of the parts pta, LuxAB (from Xenorhabdus luminescens) and BBa_K743009 VB (amplified from KanRi to RS1) we obtained the device BBa_K743014 (RS1-pta- LuxAB -KanRi-B0015-RS2). Result was confirmed by digestion.<br />
<br />
Afterwards, we amplified BBa_K743014 (from KanRi to pta) in order to exchange LuxAB from Xenorhabdus luminescens for LuxAB from Vibrio fischeri. This was accomplished by Gibson assembly obtaining BBa_K743015. Result was confirmed by digestion.<br />
<br />
The parts circadian promoter pcaa3 and psb1C3 vector backbone were amplified and further assembled by Gibson. Subsequently, cells were transformed to obtain promoter pcaa3 in BioBrick format (part: BBa_K743002). Result was confirmed by digestion.<br />
<br />
The following constructs were confirmed by sequencing:<br />
<br />
BBa_K743009<br><br />
BBa_K743004<br><br />
BBa_K743002<br><br />
BBa_K743016<br><br />
BBa_K743014<br><br />
<br />
First Synechocystis transformations were attempted using:<br />
<br />
Int_C<br><br />
BBa_K743004<br><br />
BBa_K743014 <br><br />
BBa_K743016 <br><br />
psbAB+GFP+pPMQAK1<br><br />
<br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyano/Labook/octoberTeam:UC Chile/Cyano/Labook/october2012-10-26T19:13:24Z<p>Csvidal: </p>
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<ul>Lab book:<br> <br />
<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">October</a></li><br />
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<br />
<br />
<p><font size= "5">October</font></p><br />
<br />
<br />
Synechocystis PCC 6803 cells were transformed with BBa_K743018 and BBa_K73010. Positive colonies appeared in both plates.<br><br />
<br />
A Gibson assembly and a transformation were done to modify construct BBa_K743015. The promoter pta (short) was exchanged for pta (length= 1 kb). This construct was validated by PCR and digestion.<br><br />
<br />
A Gibson assembly and a transformation were done to modify construct BBa_K743018. The promoter pta (short) was exchanged for pta (length= 1 kb). This construct was validated by PCR and digestion.<br><br />
<br />
Bioluminiscence of Synechocystis cells transformed with BBa_K743014 and BBa_K743015 was measured in a High-sensitive camera.<br />
<br />
<br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyano/Labook/octoberTeam:UC Chile/Cyano/Labook/october2012-10-26T19:13:05Z<p>Csvidal: </p>
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<ul>Lab book:<br> <br />
<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">October</a></li><br />
</ul><br />
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<br />
<br />
<p><font size= "5">October</font></p><br />
<br />
<br />
Synechocystis PCC 6803 cells were transformed with BBa_K743018 and BBa_K73010. Positive colonies appeared in both plates.<br><br><br />
<br />
A Gibson assembly and a transformation were done to modify construct BBa_K743015. The promoter pta (short) was exchanged for pta (length= 1 kb). This construct was validated by PCR and digestion.<br><br><br />
<br />
A Gibson assembly and a transformation were done to modify construct BBa_K743018. The promoter pta (short) was exchanged for pta (length= 1 kb). This construct was validated by PCR and digestion.<br><br><br />
<br />
Bioluminiscence of Synechocystis cells transformed with BBa_K743014 and BBa_K743015 was measured in a High-sensitive camera.<br />
<br />
<br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyano/Labook/septemberTeam:UC Chile/Cyano/Labook/september2012-10-26T19:12:32Z<p>Csvidal: </p>
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<ul>Lab book:<br> <br />
<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/october">October</a></li><br />
</ul><br />
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<br />
<br />
<p><font size= "5">September</font></p><br />
<br />
<br />
BBa_K743015 assembled during the last days of August was verified by sequencing.<br />
<br />
Also during the last days of August, Synechocystis PCC 6803 was transformed with:<br />
<br />
Int_C<br />
BBa_K743004<br />
BBa_K743014<br />
BBa_K743016<br />
psbAB+GFP+pPMQAK1 <br />
<br />
Only Int_C could not be transformed into cells. A colony PCR was run to amplify different sequences of BBa_K743004 and psbAB+GFP+pPMQAK1. Positive results for only for para BBa_K743004.<br />
<br />
BBa_K743014 was inoculated in a 500 mL flask to test induction by decanal. The inoculum grew in Kanamycin but did not produce bioluminescence when induced by decanal.<br />
<br />
BBa_K743004 and psbAB+GFP+pPMQAK1 was replated in Kanamycin plates but there was no growth. Construct psbAB+GFP+pPMQAK1 was replated in Kanamycin three times, but never grew. We believe it could not be transformed.<br />
<br />
We transformed Synechosystis pcc 6803 again with the following parts and now cells are being incubated at optimal conditions (continuous light and 30 °C).<br />
<br />
BBa_K743004 <br />
BBa_K743009<br />
BBa_K743016<br />
BBa_K743006<br />
BBa_K743014<br />
BBa_K743015<br />
IntC<br />
Control – (KAN)<br />
Control – (CLO)<br />
<br />
IntC could not be transformed again. The rest of the transformations seem ok.<br />
<br />
BBa_K743014 and BBa_K743016 were plated in kanamycin plates from transformation plates and a colony PCR was run for BBa_K743014 amplifying LuxAB and LuxAB with promoter.<br />
<br />
A new round of Synechocystis was run under the same conditions and constructs that the last one. <br />
<br />
The last two transformations resulted in negative controls and individual colonies for most constrcuts except for IntC (did not transform). Colonies were picked from all transformations and were replated in kanamycin plates. Our cells grew in kanamycin, which indicates they are transformed. Also, the plates were inoculated I nliquid media with kanamycin and they also grew.<br />
<br />
After multiple replatings in Kanamycin, a massive colony PCR was run of the transformed cells from the last two rounds of transformation. BBa_K743015 and BBa_K743016 transformations were verified.<br />
<br />
Due to the important amount of failed attempts to transform IntC plasmid from Utah, this was digested resulting in bands of wrong size. We sent it to sequencing.<br />
<br />
Characterization of LuxBrick was done.<br />
<br />
A growth curve was characterized for Synechosystis PCC 6803 from an OD730:0.4800 inoculum.<br />
<br />
Inoculums:<br />
3 flasks with 1 mL in 150 mL of normal BG11.<br />
1 control with 1 mL in 150 mL of BG110.<br />
*Shaker at 90 RPM.<br />
<br />
Experiment for characterization of Gibson for small parts was run.<br />
<br />
We tried to see LuxBrick induced under microscope. We concluded bioluminescence can’t be seen under microscope.<br />
<br />
We amplified parts and run a Gibson to assemble BBa_K743017 and BBa_K743018. Transformation and verification by digestion and PCR was done afterwards. Constructs were sent to sequence.<br />
<br />
Biolumiscence of different constructs with luciferase was measured when induced with different aldehides (decanal and dodecanal) using an Illuminometer.<br />
<br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyano/Labook/augustTeam:UC Chile/Cyano/Labook/august2012-10-26T19:11:53Z<p>Csvidal: </p>
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<ul>Lab book:<br> <br />
<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">October</a></li><br />
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<br />
<br />
<p><font size= "5">August</font></p><br />
<br />
During the last days of July we assembled and got PCR confirmation of BBa_K743006 (RS1-KanR-B0015-RS2). The construct was purified and now is part of our BioBrick collection.<br />
<br />
Parts C4 construct, psbAB (Synechocystis promoter) and RFP marker were assembled by Gibson and subsequently transformed. In this way we obtained BBa_K743004 (RS1-psbAB-mRFP-B0015-KanR-B0015-RS2). Confirmation was positive by PCR and digestion.<br />
<br />
Device (BBa_K743004) was transformed into cells and then purified. Later it was used to amplify its vector backbone from RS2 to mRFP. By doing this we wanted to exchange part KanR for KanRi (KanR inverse).<br />
The new amplicon was purified (VB to mRFP) and was assembled to KanRi part by Gibson, obtaining the device BBa_K743009 (RS1-psbAB-mRFP-B0015-KanRi-B0015-RS2). This new construct was transformed into cells and subsequently results were confirmed by PCR and digestion.<br />
<br />
In addition, we obtained circadian promoters pta, pcaa3 and psigE by PCR from Synechocystis DNA. LuxAB from Xenorhabdus luminescens was amplified. By assembly of the parts pta, LuxAB (from Xenorhabdus luminescens) and BBa_K743009 VB (amplified from KanRi to RS1) we obtained the device BBa_K743014 (RS1-pta- LuxAB -KanRi-B0015-RS2). Result was confirmed by digestion.<br />
<br />
Afterwards, we amplified BBa_K743014 (from KanRi to pta) in order to exchange LuxAB from Xenorhabdus luminescens for LuxAB from Vibrio fischeri. This was accomplished by Gibson assembly obtaining BBa_K743015. Result was confirmed by digestion.<br />
<br />
The parts circadian promoter pcaa3 and psb1C3 vector backbone were amplified and further assembled by Gibson. Subsequently, cells were transformed to obtain promoter pcaa3 in BioBrick format (part: BBa_K743002). Result was confirmed by digestion.<br />
<br />
The following constructs were confirmed by sequencing:<br />
<br />
BBa_K743009<br><br />
BBa_K743004<br><br />
BBa_K743002<br><br />
BBa_K743016<br><br />
BBa_K743014<br><br />
<br />
First Synechocystis transformations were attempted using:<br />
<br />
Int_C<br><br />
BBa_K743004<br><br />
BBa_K743014 <br><br />
BBa_K743016 <br><br />
psbAB+GFP+pPMQAK1<br><br />
<br />
<br />
<br />
{{UC_Chilefooter}}</div>Csvidalhttp://2012.igem.org/Team:UC_Chile/Cyano/Labook/septemberTeam:UC Chile/Cyano/Labook/september2012-10-26T19:11:27Z<p>Csvidal: </p>
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<ul>Lab book:<br> <br />
<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">October</a></li><br />
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<p><font size= "5">September</font></p><br />
<br />
<br />
BBa_K743015 assembled during the last days of August was verified by sequencing.<br />
<br />
Also during the last days of August, Synechocystis PCC 6803 was transformed with:<br />
<br />
Int_C<br />
BBa_K743004<br />
BBa_K743014<br />
BBa_K743016<br />
psbAB+GFP+pPMQAK1 <br />
<br />
Only Int_C could not be transformed into cells. A colony PCR was run to amplify different sequences of BBa_K743004 and psbAB+GFP+pPMQAK1. Positive results for only for para BBa_K743004.<br />
<br />
BBa_K743014 was inoculated in a 500 mL flask to test induction by decanal. The inoculum grew in Kanamycin but did not produce bioluminescence when induced by decanal.<br />
<br />
BBa_K743004 and psbAB+GFP+pPMQAK1 was replated in Kanamycin plates but there was no growth. Construct psbAB+GFP+pPMQAK1 was replated in Kanamycin three times, but never grew. We believe it could not be transformed.<br />
<br />
We transformed Synechosystis pcc 6803 again with the following parts and now cells are being incubated at optimal conditions (continuous light and 30 °C).<br />
<br />
BBa_K743004 <br />
BBa_K743009<br />
BBa_K743016<br />
BBa_K743006<br />
BBa_K743014<br />
BBa_K743015<br />
IntC<br />
Control – (KAN)<br />
Control – (CLO)<br />
<br />
IntC could not be transformed again. The rest of the transformations seem ok.<br />
<br />
BBa_K743014 and BBa_K743016 were plated in kanamycin plates from transformation plates and a colony PCR was run for BBa_K743014 amplifying LuxAB and LuxAB with promoter.<br />
<br />
A new round of Synechocystis was run under the same conditions and constructs that the last one. <br />
<br />
The last two transformations resulted in negative controls and individual colonies for most constrcuts except for IntC (did not transform). Colonies were picked from all transformations and were replated in kanamycin plates. Our cells grew in kanamycin, which indicates they are transformed. Also, the plates were inoculated I nliquid media with kanamycin and they also grew.<br />
<br />
After multiple replatings in Kanamycin, a massive colony PCR was run of the transformed cells from the last two rounds of transformation. BBa_K743015 and BBa_K743016 transformations were verified.<br />
<br />
Due to the important amount of failed attempts to transform IntC plasmid from Utah, this was digested resulting in bands of wrong size. We sent it to sequencing.<br />
<br />
Characterization of LuxBrick was done.<br />
<br />
A growth curve was characterized for Synechosystis PCC 6803 from an OD730:0.4800 inoculum.<br />
<br />
Inoculums:<br />
3 flasks with 1 mL in 150 mL of normal BG11.<br />
1 control with 1 mL in 150 mL of BG110.<br />
*Shaker at 90 RPM.<br />
<br />
Experiment for characterization of Gibson for small parts was run.<br />
<br />
We tried to see LuxBrick induced under microscope. We concluded bioluminescence can’t be seen under microscope.<br />
<br />
We amplified parts and run a Gibson to assemble BBa_K743017 and BBa_K743018. Transformation and verification by digestion and PCR was done afterwards. Constructs were sent to sequence.<br />
<br />
Biolumiscence of different constructs with luciferase was measured when induced with different aldehides (decanal and dodecanal) using an Illuminometer.<br />
<br />
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{{UC_Chilefooter}}</div>Csvidal