"

From 2012.igem.org

Combined display of all available logs of 2012.igem.org. You can narrow down the view by selecting a log type, the user name (case-sensitive), or the affected page (also case-sensitive).

Logs

All public logs Block log Deletion log Import log Merge log Move log Patrol log Protection log Upload log User creation log User rights log User:  Title:   Search titles starting with this text

From year (and earlier): From month (and earlier): all January February March April May June July August September October November December

• 02:39, 27 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne Berlin conference.jpg" ‎ (June presenting our poster at a conference)
• 21:38, 26 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-09-15 RO-BB failed PCR.jpg" ‎ (This is a PCR of the readout with added BioBrick tails. The PCR has apparently failed.)
• 10:51, 26 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne pcDNA(-)-LovTAP 31.08.12.JPG" ‎ (Colony PCR gel)
• 09:12, 26 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-24-08 colony PCR rerun in CH.jpg" ‎ (Edited version of the gel.)
• 23:00, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne Team-EPF-Lausanne 2012.09.01 pGL-SEAP colony PCR.jpg" ‎ (Only one of these colonies actually has the correct plasmid.)
• 17:22, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-17 failed colony PCR.jpg" ‎ (In a nutshell: a colony PCR that has failed.)
• 17:03, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne presentation ssbe.pdf" ‎ (The 15-min presentation we gave at the SSBE conference on the 27th of August at EPFL.)
• 17:00, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne SSBE poster about our project.pdf" ‎ (The poster we presented at the SSBE conference on the 27th of August at EPFL.)
• 16:09, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-24 colony PCR rerun.jpg" ‎ (Gel from the CH lab to check the weird colony PCR results again.)
• 12:28, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-23 LovTAP readout gel extraction.jpg" ‎ (Gel of two different digestions of the LovTAP readout (BamHI/KpnI, XhoI/KpnI). For extraction.)
• 09:26, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-19 Enzyme testing.jpg" ‎ (Enzyme tests.)
• 08:56, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-20 test PCR in BM lab.jpg" ‎ (PCR on diverse LovTAP templates, and an eGFP control (one by Sowmya, one by Matt).)
• 07:30, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-14 Gel PCR products(debugging everything)+digestion of supposed LovTAP-pMP.jpg" ‎ (PCR products (comparing HF and GC buffers for all of our PCRs), pMP-LovTAP (turned out to be pMA-LovTAP) digestion with NotI and SpeI.)
• 07:07, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-12 supposed pMP-LovTAP digested with EcoRI-HF.jpg" ‎ (Restriction digestion check of ligation of LovTAP into pMP.)
• 06:57, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-11 supposed pGL-SEAP digested with NdeI.jpg" ‎ (Digestion of the pGL-SEAP ligation with NdeI.)
• 06:44, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-11 supposed pGL-TNFR digested with EcoNI.jpg" ‎ (Digestions of a ligation of pGL-TNFR with EcoNI, ran on a gel, failed. But then again, the enzyme might be old.)
• 01:08, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-09 gel purification LovTAP-pMA and pGL.jpg" ‎ (Digestion of the LovTAP-pMA plasmid (to excise LovTAP and to ligate it into pcDNA3.1(+)) and of the pGL plasmid (to use for ligations with Fussenegger readouts). On a gel (duh).)
• 01:01, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-09 supposed TNFR-pGL ligations.jpg" ‎ (Digestions of TNFR ligations to see if they actually ligated. They didn't.)
• 00:24, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-08 LovTAP for pMP+BB melanopsin ROs.jpg" ‎ (PCRs for LovTAP restriction site insertion from 2 different days, attempt to make a BioBricking PCR work)
• 00:11, 25 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-08 Digestions of supposed pGL-SEAP and pGL-GFP.jpg" ‎ (Miniprepped ligations that were digested and run on a gel to show that the ligations didn't work.)
• 23:19, 24 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-06 PCR products of melanopsin readouts.jpg" ‎ (A gel with PCR products of TNFR, eGFP and SEAP (readouts for the Fussenegger experiment).)
• 23:32, 23 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-07-23 western membrane.jpg" ‎ (A Western blot for the detection of LovTAP with an anti-VP16 antibody.)
• 00:52, 23 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne 2012-08-10 Restriction site+BB PCRs, TNFR+pGL ligation.jpg" ‎ (A gel of many PCRs, most of which had failed)
• 00:49, 18 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne-2012-09-16 RO BB.png" ‎ (Gel of the BioBricking LovTAP readout PCR. This time, it finally worked!)
• 00:39, 18 September 2012 Aiourano (Talk | contribs) moved Team:EPF-Lausanne/Modeling/lovtap to Team:EPF-Lausanne/Modeling/LovTAP ‎ (Capitals :))
• 00:49, 17 September 2012 Aiourano (Talk | contribs) uploaded a new version of "File:Team-EPF-Lausanne HEK Transfection excel protocol.xls" ‎ (Changed 2h post-transfection to 3h)
• 00:41, 17 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne HEK Transfection excel protocol.xls" ‎ (Excel protocol for the transfection of HEK cells. Fill in the concentrations and percentages that are in red, the file will calculate the required DNA volume for you. Print the sheet out and follow the provided protocol. You're welcome.)
• 00:12, 17 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne CHO Transfection excel protocol.xls" ‎ (Excel protocol for the transfection of CHO cells. Fill in the concentrations and percentages that are in red, the file will calculate the required DNA volume for you. Print the sheet out and follow the provided protocol. You're welcome.)
• 17:42, 13 September 2012 Aiourano (Talk | contribs) uploaded "File:Team-EPF-Lausanne Flash article about us.pdf" ‎ (Article (in French) about iGEM in general and our project in particular, printed in the EPFL internal journal (devoted to research and campus news), written by a team member and reviewed by the other ones.)
• 13:28, 13 September 2012 Aiourano (Talk | contribs) moved Team:EPF-Lausanne/Protocol/Manual Counting by Heamocytometer- Trypan Blue Method to Team:EPF-Lausanne/Protocol/TrypanBlue ‎
• 22:24, 12 September 2012 Aiourano (Talk | contribs) moved Team:EPF-Lausanne/Protocol/New Western Blot to Team:EPF-Lausanne/Protocol/Western Blot ‎ (Who the hell named it like that in the first place?)