"

From 2012.igem.org

Note: Professor Isaacs brought samples of B. subtilis 1A40 from the Breaker Lab. There was 1 plate and 1 liquid culture

I: Make 15% Glycerol Stocks (4) from B. subtilis 1A40 Liquid Culture from Breaker Lab (Keith)

• Chill an aliquot of sterile 60% glycerol on ice
• Transfer 375 uL of liquid culture to each microcentrifuge tube (x4)
• Add 125 uL of 60% glycerol to each tube (x4)
• Vortex to mix thoroughly
• Store tubes (purple) in the -80 ^oC freezer
• Store plate and remaining liquid culture in the 4 ^oC deli refrigerator