Team:Washington/Protocols/sequencing
From 2012.igem.org
DNA Sequencing
- Add 10uL of miniprep DNA to an PCR Strip Tubes. The only labels on the Tube should be JS01 ... JSXX (depending on the number of samples)
- Go to Genewiz Customer Login
- Click Place and order --> Create sequencing order
- Click Same Day --> Online Form --> Pre-Defined --> Enter # of Samples and then Create New Form
- Enter your DNA Name (make sure it is not redundant and in order of wells you are submitting)
- DNA TYpe --> "Plasmid" (click the Type button to autofill the rest, same for all others)
- DNA Length --> "6-10kb"
- DNA Conc --> "50" (even if this is not true... thats ok)
- Primer Name --> "VF2_iGEM" or "VR_iGEM" (if you want forward or reverse, respectively)
- type "Primers are stored at GENEWIZ" in the comment area
- Click Save and Next
- Print out receipt (Send to an advisor and we can print it out) and place labeled samples (JS01 ... JSXX) and order sheet in a Plastic Bag and then DropBox
- When sequencing is completed you can view the results by logging in going to the appropriate link under "Recent Orders" on the lower right side of the screen