"
Team:LMU-Munich/Spore Coat Proteins
From 2012.igem.org
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Sporobeads - What Protein Do You Want to Display?
Sporobeads are the first generation of Bacillus subtilis endospores displaying a protein of our choice on their outermost layer, the spore crust. In future Sporobeads could serve as a platform for protein display and thus be used for numerous versatile applications. Our goal was to show that B. subtilis spores have the ability to do so. As a proof of principle we successfully fused GFP to the spore crust and obtained fluorescence with microscopy.
Scientific Background
Introduction to B. subtilis spores and their use in our project |  |
| |
|---|---|
Cloning Strategy
Cloning strategy to create different variants of our Sporobeads | 
|
|
| |
|---|---|
GFP as a Proof of Principle
Main results of the various constructs that were created to find the best one! |  |
|
| |
|---|---|
Laccases as functional enzymes
Creation of functional Laccase- Sporobeads |
|
|
| |
|---|---|
Purification Methods
Description of the different purification methods of the spores |  |
|
| |
|---|---|
| 
|
| 
|
| Bacillus Intro | Bacillus BioBrickBox | Sporobeads | Germination STOP |
