How the Flip & Flop Works

1. Main Page - Bacteriophage Bxb1 gp35, DNA integrase(Bxb1_Int), BBa_K907000:
   A serine integrase family protein which recognizes specific DNA sequences and recombines dsDNA.


2. Main Page - Binary Signal Generator, RBS(reverse) - attB - Promoter - attP – RBS, BBa_K907002:
   Promotes transcription and translation of two different proteins one at a time when work with BBa_K907000 and BBa_K907001 to generate binary signal.


3. Main Page - Dual Phase Protein Generator (GFP default), BBa_K907004:
   Derivative of BBa_K907002 mRFP and GFP are attached to both ends of BBa_K907002 to generate binary signal via fluorescent proteins.

1. Experience – Highly engineered mutant of red fluorescent protein from Discosoma striata (coral), BBa_E1010 (Endy Lab, 2004):
   BBa_E1010 was successfully used to produce mRFP in E.coli strain MG1655 in LB or M9 minimal media under the control of BBa_K907005.


2. Experience – Green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Endy Lab, 2004):
   BBa_E0040 was successfully used to produce GFP in E.coli strain MG1655 in LB or M9 minimal media under the control of BBa_K907004.