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From 2012.igem.org

Preparation for Electroporation

• Inoculate cells into 10mL of LB media (scrape glycerol stock with metal loop and place into LB solution in culture tube)

• ADP1, EcNR2, BS168, BS PERM739, BS PY79, BS 1A833, XL1-Blue

• Place culture tubes in shaking incubator
• Transfer solution to 125mL E.flasks and add 15mL of LB media

• Inoculate cells onto appropriate LB plates for overnight growth

• same cells minus XL1-Blue