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From 2012.igem.org

Kanomycin Plate Preparation

• Autoclave two 500mL LB-Agar solutions
• Add 0.5mL of stock kanomycin (50mg/mL) in one 500mL LB-Agar solution (final conc. 50ug/mL)
• Add 0.2mL of stock kanomycin (50mg/mL) in other 500mL LB-Agar solution (final conc. 20ug/mL)
• Pour our solutions into 20 plates each
• Wait for them to cool, then flip and bag
• Store in deli fridge

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Lab Notes, August, 6, 2012

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Electroporation Preparation - Buffers

• Prepare electroporation and washing buffers for B.Subtilis
• Washing Buffer

• 0.25M Sucrose
• 1mM Hepes
• MgCl2
• (pH - 7.0)

• Electroporation Buffer

• 0.3M Sucrose
• 1mM CaCl2
• 1mM Sodium citrate
• (pH - 6.0)