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From 2012.igem.org

Contents 1 I. Finished the presentation powerpoint 2 II. Continued freeze-thaw selection pressure tests for MAGE including plating of solution samples 3 III. Measured approximate concentrations of DNA in PCR reactions used to transform cells the previous day to find out that dsDNA concentration in reaction 9+10 and 29+30 are very low 4 IV. Nevertheless, redid transformation of DHA alpha cells using the same three PCR reactions (5 microliters each) but as the previous day used LB instead of probably the more effective SOC 5 V. Plated the solutions on amp + kan plates 6 VI. Presented RiAFP topic and received feedback about future work

I. Finished the presentation powerpoint

II. Continued freeze-thaw selection pressure tests for MAGE including plating of solution samples

III. Measured approximate concentrations of DNA in PCR reactions used to transform cells the previous day to find out that dsDNA concentration in reaction 9+10 and 29+30 are very low

IV. Nevertheless, redid transformation of DHA alpha cells using the same three PCR reactions (5 microliters each) but as the previous day used LB instead of probably the more effective SOC

V. Plated the solutions on amp + kan plates

VI. Presented RiAFP topic and received feedback about future work