From 2012.igem.org
Cyanolux & Bactomithril - Pontificia Universidad Católica de Chile, iGEM 2012
March 19 - March 25
Thursday
- Expression plasmid pPMQAK1 (received from Sweden in February) was left growing.
- Brick J33210 was transformed in a broad host plasmid.
- New batch of competent E. coli (E. cloni) was prepared.
- Mipreps pPMQAK1 and pSB4K5
- Primers in for C1 and others (primers designed in meeting 1)
- Synechocystis PCC6803 culture was examined under confocal microscopy. The culture was not axenic.
- A designed construct was wrong. Included a template that was not in the kit 2011 (KanR + Double terminator, part K292003)
- Part K292003 was built by standard assembly (B1003+B1004)
Sunday
- Biobrick assembly protocol according to Registry Protocol. Assembly: B1003+B1004 in pSB1C3.
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