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Team:Lyon-INSA/datapage
From 2012.igem.org
Interactive pattern of our construction : Toggle switch option
Our parts :
Data about our favorite new parts
1. Main Page: Dispersin generator for B. subtilis BBa_K802001 : This part associates the Bacillus subtilis constitutive promoter (Pveg) with the dispersin B gene (dspB). This part allows to efficiently scatter Staphylococci biofilms. 2. Main Page: Lysostaphin generator for B. subtilis BBa_K802000 : This part associates the Bacillus subtilis constitutive promoter (Pveg) with the lysostaphin gene. This part allows an efficient killing of S. aureus cells.
3. Main Page: Surfactin generator and biofilm repressor for B. subtilis BBa_K802009: This part can be used to induce surfactin production and to repress the biofilm formation in B. subtilis strains (COAT module).NEW
We've also characterized the following new parts
Main Page: Plac-RBS-GFP BBa_K802002 : This part has been designed to determine the behavior of the Plac promoter used to drive the STICK module (Part BBa_K802009)
Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802003 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a low copy number plasmid in B. subtilis and a high copy number plasmid in E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in B. subtilis by a transformation yield of 70 transformants/µg and a erythromycin resistance up to 900 µg/mL.
Main Page: Shuttle vector for E. coli and B. subtilis BBa_K802004 : This part is a shuttle vector of 6.5kb which is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high copy number plasmid in both B. subtilis and E. coli. It has a polylinker containing all 4 of the iGEM restriction sites. This vector is characterized in B. subtilis by a transformation yield of 80 transformants/µg and a erythromycin resistance to at least 1.5 mg/mL.
Main Page: Biofilm repressor for B. subtilis strains BBa_K802007 : This part can be used to impede biofilm formation in B. subtilis, more particularly if it is arbB-. This part was characterized in conjuction with parts K802006 and K802008.NEW
