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Team:LMU-Munich/Bacillus BioBricks/Vectors
From 2012.igem.org
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
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Bacillus Vectors 
We have generated a suite of BioBrick-compatible vectors: three empty insertional backbones with different antibiotic resistances and integration loci, two reporter and two expression vectors. Here is a list of all the vectors we cloned and used.
| Vector Name | Resistance | Insertion | Description | Vector origin | ||
|---|---|---|---|---|---|---|
| BioBrick | Eco | Bsu | locus | Name | Reference | |
| pSBBs1C [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823023 (BBa_K823023)] | Amp | Cm | amyE | empty | pDG1662 | [http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury] |
| pSBBs4S [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823022 (BBa_K823022)] | Amp | Spec | thrC | empty | pDG1731 | [http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury] |
| pSBBs2E [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823027 (BBa_K823027)] | Amp | MLS | lacA | empty | pAX01 | [http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl] |
| pSBBs1C-lacZ [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823021 (BBa_K823021) ] | Amp | Cm | amyE | lacZ reporter | pAC6 | [http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke] |
| pSBBs3C-luxABCDE [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 (BBa_K823025)] | Amp | Cm | sacA | luxABCDE reporter | pAH328 | [http://www.ncbi.nlm.nih.gov/pubmed/20709900 Schmalisch] |
| pSBBs4S-PXyl [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823024 (BBa_K823024)] | Amp | Spec | thrC | Xylose-promoter | pXT | [http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré] |
| pSBBs0K-Pspac [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 (BBa_K823026)] | Amp | Kan | replicative | IPTG-promoter | pDG148 | [http://www.ncbi.nlm.nih.gov/pubmed/11728721 Joseph] |
| Sporovector [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823054 (BBa_K823054)] | Amp | Spec | thrC | to create Sporobeads | pSBBs4S | Sporovector |
For the use of our vectors please see our Protocols page. A general introduction to Bacillus subtilis and its integrative vectors can be found here. All vectors have ampicillin as Escherichia coli resistance and RFP in the multiple cloning site as selection marker.
The corresponding vector maps:
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The number in the vector's name codes for the insertion locus and the following letter for the Bacillus subtilis resistance gene according to the following table:
| Number | Insertion locus | Letter | Resistance |
|---|---|---|---|
| 0 | replicative | C | Chloramphenicol (Cm) |
| 1 | amyE (amylase) | E | MLS (Erythromycin + Lincomycin) |
| 2 | lacA (β-galactosidase) | K | Kanamycin (Kan) |
| 3 | sacA (sucrase) | S | Spectinomycin (Spec) |
| 4 | thrC (threonine synthase) |
The concentrations of the antibiotics for selection of B.subtilis transformation and tests for plasmid insertion can be found in our Protocol section.
See here to find out how to use B. subtilis vectors. In this overview, the integration mechanism of B. subtilis vectors is described.
The design and special use of our Sporovector can be found here.
For results, check out our Data page: 
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